[Federal Register Volume 91, Number 157 (Monday, August 17, 2026)]
[Rules and Regulations]
[Pages 53184-53191]
From the Federal Register Online via the Government Publishing Office [www.gpo.gov]
[FR Doc No: 2026-16727]


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DEPARTMENT OF HEALTH AND HUMAN SERVICES

Food and Drug Administration

21 CFR Part 864

[Docket No. FDA-2025-N-1243]


Hematology and Pathology Devices; Reclassification of In Situ 
Hybridization Test Systems for Use With a Corresponding Approved 
Oncology Therapeutic Product

AGENCY: Food and Drug Administration, HHS.

ACTION: Final amendment; final order.

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SUMMARY: The Food and Drug Administration (FDA, the Agency, or we) is 
issuing a final order reclassifying in situ hybridization (ISH) test 
systems indicated for use with a corresponding approved oncology 
therapeutic product (product codes NYQ, MVD, OWE, and PNK), all 
postamendments class III (premarket approval) devices, into class II 
(special controls), subject to premarket notification. FDA is also 
establishing a new device classification regulation, along with the 
special controls that are necessary to provide a reasonable assurance 
of safety and effectiveness of this device type.

DATES: This order is effective September 16, 2026. See further 
discussion in section IV, ``Implementation Strategy.''

FOR FURTHER INFORMATION CONTACT: Soma Ghosh, Center for Devices and 
Radiological Health, Food and Drug Administration, 10903 New Hampshire 
Ave., Bldg. 66, Rm. 3316, Silver Spring, MD 20993, 240-402-5333, 
[email protected].

SUPPLEMENTARY INFORMATION:

I. Background--Regulatory Authorities

    The Federal Food, Drug, and Cosmetic Act (FD&C Act), as amended, 
establishes a comprehensive system for the regulation of medical 
devices intended for human use. Section 513 of the FD&C Act (21 U.S.C. 
360c) established three classes of devices, reflecting the regulatory 
controls needed to provide reasonable assurance of their safety and 
effectiveness. The three classes of devices are class I (general 
controls), class II (special controls), and class III (premarket 
approval).
    Devices that were not introduced or delivered for introduction into 
interstate commerce for commercial distribution prior to May 28, 1976 
(generally referred to as postamendments devices) are automatically 
classified by section 513(f)(1) of the FD&C Act into class III without 
any FDA rulemaking process. Those devices remain in class III and 
require approval of a premarket approval application (PMA), unless and 
until: (1) the Food and Drug Administration (FDA) reclassifies the 
device into class I or class II; or (2) FDA issues an order finding the 
device to be substantially equivalent, in accordance with section 
513(i) of the FD&C Act, to a predicate device that does not require 
premarket approval. FDA determines whether new devices are 
substantially equivalent to predicate devices by means of the 
procedures in section 510(k) of the FD&C Act (21 U.S.C. 360(k)) and our 
implementing regulations (part 807, subpart E (21 CFR part 807, subpart 
E)).
    A postamendments device that has been initially classified into 
class III under section 513(f)(1) of the FD&C Act may be reclassified 
into class I or class II under section 513(f)(3) of the FD&C Act. 
Section 513(f)(3) of the FD&C Act provides that FDA, acting by 
administrative order, can reclassify the device into class I or class 
II on its own initiative, or in response to a petition from the 
manufacturer or importer of the device. To change the classification of 
the device, the new class must have sufficient regulatory controls to 
provide reasonable assurance of the safety and effectiveness of the 
device for its intended use.\1\
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    \1\ See section 513 of the FD&C Act.
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    FDA relies upon ``valid scientific evidence,'' as stated in section 
513(a)(3) of the FD&C Act and defined in 21 CFR

[[Page 53185]]

860.7(c)(2), in the classification process to determine the level of 
regulation for devices. To be considered in the reclassification 
process, the ``valid scientific evidence'' upon which the Agency relies 
generally must be publicly available. Publicly available information 
excludes trade secret and/or confidential commercial information, e.g., 
the contents of a pending PMA (see section 520(c) of the FD&C Act (21 
U.S.C. 360j(c))). Section 520(h)(4) of the FD&C Act (21 U.S.C. 
360j(h)(4)) provides that FDA may use, for reclassification of a 
device, certain information in a PMA 6 years after the application has 
been approved. This includes information from clinical and preclinical 
tests or studies that demonstrate the safety and effectiveness of the 
device, but it does not include the descriptions of methods of 
manufacture and product composition and other trade secrets.\2\
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    \2\ Since the publication of the proposed order, data from one 
additional PMA and several PMA supplements have become available for 
consideration by FDA in accordance with section 520(h)(4) of the 
FD&C Act. FDA has determined that the data from the additional PMA 
and PMA supplements are cumulative of, and consistent with, the 
information addressed in the proposed order and do not raise any new 
or different questions regarding the safety and effectiveness of 
oncology therapeutic ISH-based test systems.
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    Section 510(m) of the FD&C Act provides that FDA may exempt a class 
II device from the requirements under section 510(k) of the FD&C Act if 
FDA determines that a premarket notification (510(k)) is not necessary 
to provide reasonable assurance of the safety and effectiveness of the 
device type.
    On June 11, 2025, FDA published a proposed order \3\ in the Federal 
Register (90 FR 24540) (``proposed order'') to reclassify in situ 
hybridization (ISH) test systems indicated for use with a corresponding 
approved oncology therapeutic product (product codes NYQ, MVD, OWE, and 
PNK) \4\ (hereinafter referred to as oncology therapeutic ISH-based 
test systems) from class III to class II. FDA has considered the 
information available to the Agency, as described in the June 11, 2025, 
proposed order, and considered comments received from the public docket 
on the proposed order (as discussed in section II of this document), to 
determine that there is sufficient information to establish special 
controls to effectively mitigate the risks to health (updated as 
discussed in section II of this document). FDA has also determined 
based on this information that the special controls, together with 
general controls, provide a reasonable assurance of safety and 
effectiveness when applied to these devices.
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    \3\ FDA notes that the ``ACTION'' caption for the proposed order 
was styled as ``Proposed amendment; proposed order; request for 
comments,'' rather than ``Proposed order.'' Beginning in December 
2019, this editorial change was made to indicate that the document 
``amends'' the Code of Federal Regulations. The change was made in 
accordance with the Office of Federal Register's (OFR) 
interpretations of the Federal Register Act (44 U.S.C. chapter 15), 
its implementing regulations (1 CFR 5.9 and parts 21 and 22), and 
the Document Drafting Handbook.
    \4\ FDA's Center for Devices and Radiological Health (CDRH) uses 
product codes to assist in accurate identification and tracking of 
current medical devices and to allow for tracking of and easy 
reference to predicate device types. A medical device product code 
consists of a three-letter combination which associates a device's 
type with a product classification designated for the application. 
The three-digit classification product codes in CDRH's Product 
Classification Database carry no other significance. See FDA 
guidance titled ``Medical Device Classification Product Codes'' 
available at https://www.fda.gov/regulatory-information/search-fda-guidance-documents/medical-device-classification-product-codes-guidance-industry-and-food-and-drug-administration-staff.
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    Therefore, in accordance with section 513(f)(3) of the FD&C Act, 
FDA, on its own initiative, is issuing this final order to reclassify 
oncology therapeutic ISH-based test systems from class III to class II 
(special controls).\5\ Absent the special controls identified in this 
final order, general controls applicable to the device type are 
insufficient to provide a reasonable assurance of safety and 
effectiveness. Specifically, general controls are insufficient to 
effectively mitigate the risks identified for this device type, such as 
the risk of false test results (i.e., false positive and false negative 
test results), which may negatively influence treatment decisions for 
cancer patients--for example, by delaying access to an available and 
appropriate alternative therapy. FDA expects that the reclassification 
of these devices will enable more manufacturers to develop this type of 
device such that patients will benefit from increased access to 
oncology therapeutic ISH-based test systems for which there is a 
reasonable assurance of safety and effectiveness.
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    \5\ FDA notes that the ``ACTION'' caption for this final order 
is styled as ``Final amendment; final order,'' rather than ``Final 
order.'' Beginning in December 2019, this editorial change was made 
to indicate that the document ``amends'' the Code of Federal 
Regulations. The change was made in accordance with the Office of 
Federal Register's (OFR) interpretations of the Federal Register Act 
(44 U.S.C. chapter 15), its implementing regulations (1 CFR 5.9 and 
parts 21 and 22), and the Document Drafting Handbook.
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    After the effective date of this order, for these class II devices, 
manufacturers may submit a premarket notification and obtain FDA 
clearance of the devices before marketing them, as opposed to having to 
submit a PMA and receive approval. This action will decrease regulatory 
burden on industry, as manufacturers will no longer have to submit a 
PMA for this type of device but can instead submit a 510(k) to the 
Agency for review prior to marketing their device. A 510(k) typically 
results in a shorter premarket review timeline compared to a PMA, which 
ultimately provides patients with more timely access to this type of 
device.

II. Comments on the Proposed Order and FDA Responses

A. Introduction

    FDA received comments from fewer than 5 commenters on the proposed 
order published in the Federal Register on June 11, 2025. The comment 
period on the proposed order closed on August 11, 2025. The majority of 
the comments received by the close of the comment period came from 
members of the medical device industry. Some commenters provided 
multiple comments on one or more issues. All commenters provided 
support for the proposed reclassification with some comments also 
providing recommendations or proposed modifications for clarity.
    We describe and respond to the comments in section II.B of this 
document. The order of the comments and our response to them is purely 
for organizational purposes and does not signify the comment's value or 
importance nor the order in which comments were received. Certain 
comments are grouped together under a single number because the subject 
matter is similar. Please note that in some cases we separated 
different issues discussed by the same commenter and designate them as 
distinct comments for purposes of our responses.

B. Description of Comments and FDA Response

    (Comment 1) FDA received numerous comments supporting the proposed 
reclassification of oncology therapeutic ISH-based test systems, from 
class III to class II, subject to special controls. Citing, among other 
things, ISH as a long-established technology, commenters stated they 
believe that special controls could be established to provide 
reasonable assurance of the safety and effectiveness of these devices. 
In addition, commenters noted that the decreased regulatory burden 
resulting from the reclassification could increase development and 
availability of these tests, thus offering better diagnostic options 
for clinicians and improving patient access to diagnostics and 
therapies.

[[Page 53186]]

    (Response 1) FDA agrees with the comments supporting this 
reclassification. Based on the information the Agency considered and 
analyzed in proposing to reclassify these devices, as well as comments 
received in response to the proposed order, FDA has determined that 
reclassifying oncology therapeutic ISH-based test systems from class 
III (premarket approval) into class II (special controls) is 
appropriate. Specifically, based on the totality of information 
available, FDA has determined that general controls are insufficient to 
provide a reasonable assurance of safety and effectiveness for these 
devices and there is sufficient information to establish special 
controls for these devices that together with general controls will 
provide a reasonable assurance of safety and effectiveness. In 
addition, FDA also expects that the reclassification of these devices 
will enable more manufacturers to develop this type of device such that 
health care providers and patients will benefit from increased access 
to appropriately safe and effective tests.
    (Comment 2) One commenter requested the Agency's guidance and 
further deliberation on these matters, either preceding or during a 
reclassification panel meeting.
    (Response 2) FDA has determined that a classification panel is 
unnecessary to reclassify oncology therapeutic ISH-based test systems 
from class III to class II. Section 513(f)(3) of the FD&C Act provides 
that FDA may ask an appropriate panel to review information and make a 
recommendation before issuing an order reclassifying a postamendments 
device (see also 21 CFR 860.134(c)(2)). FDA is reclassifying these 
postamendments class III devices on its own initiative and does not 
believe a panel recommendation is needed to help determine whether 
these devices should be reclassified from class III to class II nor to 
identify appropriate special controls. FDA has determined, based on the 
information discussed in the preamble to the proposed order and FDA's 
consideration of public comments, that the standard in section 
513(a)(1)(B) of the FD&C Act is met. Specifically, FDA has determined 
that general controls are insufficient to provide a reasonable 
assurance of safety and effectiveness, and there is sufficient 
information to establish special controls, which with general controls 
will provide a reasonable assurance of the safety and effectiveness 
when applied to these devices.
    (Comment 3) One commenter expressed appreciation for the Agency's 
use of the least burdensome approach through discussion of 
predetermined change control plans (PCCPs) in the preamble to the 
proposed order. The commenter requested the Agency allow applicants to 
seek FDA's alignment on device-specific PCCPs via pre-submission rather 
than a pre-market notification and further requested that the Agency 
consider permitting PCCPs for a planned modification to already PMA-
approved devices to include artificial intelligence (AI)/machine 
learning (ML) driven digital pathology (DP) algorithms intended to aid 
the end user or seek aid from the end user for end result generation, 
via PCCPs.
    (Response 3) The new classification regulation at Sec.  864.1890 
(21 CFR 864.1890) applies to previously approved oncology therapeutic 
ISH-based test systems and new devices that are determined to be 
substantially equivalent. We note that, at the time of publication of 
this final order, FDA has not classified, cleared, approved, or granted 
authorization for any oncology therapeutic ISH-based test systems that 
incorporate DP devices, including AI/ML algorithm-assisted DP devices.
    As described in the preamble of the proposed order, manufacturers 
may wish to use PCCPs as a way to implement future modifications to 
their devices without needing to submit a new 510(k) for each 
significant change or modification \6\ while continuing to provide 
reasonable assurance of device safety and effectiveness. FDA reviews a 
PCCP as part of a marketing submission for a device to ensure the 
continued safety and effectiveness of the device without necessitating 
additional marketing submissions for implementing each modification 
described in the PCCP (see section 515C of the FD&C Act (21 U.S.C. 
360e-4)). Thus, FDA's consideration of a device-specific PCCP, and the 
determination of whether the inclusion of a device modification, to 
include a modification to an AI/ML-enabled device via a PCCP is 
appropriate, will be based on the Agency's review and consideration of 
the information submitted at the time of a premarket submission. FDA 
encourages manufacturers to leverage the Q-Submission program to obtain 
FDA feedback on their approach to using a PCCP for a device prior to 
submitting a marketing submission. Additional information regarding the 
Q-Submission program can be found in FDA's final guidance document 
titled ``Requests for Feedback and Meetings for Medical Device 
Submissions: The Q-Submission Program'' (Ref. 1). For additional 
information regarding marketing submissions that include a PCCP for AI/
ML-enabled devices, see FDA's guidance, ``Marketing Submission 
Recommendations for a Predetermined Change Control Plan for Artificial 
Intelligence-Enabled Device Software Functions'' (Ref. 2).
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    \6\ For the purpose of this final order reference to 
``modification'' means a significant change or modification that 
would generally require a new premarket notification under Sec.  
807.81(a)(3).
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    (Comment 4) One comment recommended the Agency reference and 
``include the principles'' of FDA's guidance, ``Replacement Reagent and 
Instrument Family Policy for In Vitro Diagnostic Devices'' in this 
final order.
    (Response 4) FDA appreciates this comment and acknowledges that the 
guidance ``Replacement Reagent and Instrument Family Policy for In 
Vitro Diagnostic Devices'' may be applicable under certain 
circumstances to oncology therapeutic ISH-based test systems (Ref. 3). 
When evaluating the applicability of this guidance to specific 
modifications to a particular test system, interested parties should 
follow the logic scheme and recommendations contained in the guidance.
    (Comment 5) One commenter requested several changes to the proposed 
classification regulation. The commenter recommended revising the 
proposed codified text for Sec.  864.1890 by adding a new paragraph 
titled ``Device Description and Principle of Operation,'' as Sec.  
864.1890(b). The suggested language would require, presumably in a 
submission to FDA and/or in the device labeling, detailed elements of a 
device's intended use/indication for use including specifications for 
the intended use population(s), biomarker lists, specimen type(s), 
system components, biomarker definitions as used in the therapeutic 
product trials and as detected by the test, along with the 
corresponding therapeutic product(s) or therapeutic product group, and 
a description of whether the device is qualitative, semi-quantitative, 
or quantitative. The commenter also proposed renumbering the 
identification provision as paragraph (a) and classification provision 
as paragraph (c).
    The commenter also recommended adding a new labeling special 
control, to be incorporated as Sec.  864.1890(c)(2)(i), which would 
require Sec.  809.10 (21 CFR 809.10) compliant labeling and any product 
information and test output generated to include the intended use 
statement as outlined in the commenter's proposed Sec.  864.1890(b).
    (Response 5) While FDA agrees that labeling special controls, in 
addition to general controls, are needed to assure the safety and 
effectiveness of oncology therapeutic ISH-based test systems, FDA

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disagrees that the commenter's proposed edits to Sec.  864.1890 are 
necessary. FDA believes the labeling special controls proposed by the 
Agency, in combination with the applicable general controls, which 
include general in vitro diagnostic (IVD) product labeling requirements 
under Sec.  809.10, are sufficient for the labeling of this device 
type.
    FDA declines to adopt the proposed revisions at Sec.  864.1890(b) 
to the codified text. Much of this information is already required to 
be submitted to FDA under existing general controls. For example, Sec.  
809.10(b)(2) requires the type of procedure to be included in labeling 
(e.g., qualitative or quantitative) and Sec.  807.87(e) requires 
submission of the device's proposed labeling as part of a 510(k). FDA 
does not believe that an additional requirement mandating submission of 
all of this information is necessary for all devices in this device 
type. Moreover, and as discussed further below, FDA does not believe 
all of this information needs to be included in the device's intended 
use/indications for use statement found in the device's labeling to 
provide reasonable assurance of the safety and effectiveness of these 
devices. Accordingly, FDA is not adding the proposed paragraph to Sec.  
864.1890, nor is FDA renumbering the identification and classification 
paragraphs as proposed.
    FDA also declines to incorporate the commenter's proposed edits to 
the labeling special control. The suggested revisions would require 
labeling to include an intended use statement referencing the 
commenter's proposed Sec.  864.1890(b), which FDA is not adopting for 
the reasons described in the previous paragraph. Moreover, the 
commenter's suggested requirements duplicate or overlap with existing 
regulatory requirements under Sec.  809.10 and part 801 (21 CFR part 
801), as well as the labeling elements already encompassed by the 
Agency's proposed special controls. The Agency's proposed special 
controls, along with applicable statutory and regulatory requirements, 
address all of the necessary intended use statements and operational 
descriptions (e.g., summary and explanation of the test) that are to be 
included in labeling, without the need for the additional codified 
requirements. FDA believes the special controls, as finalized, along 
with general controls, will ensure the device's risks to health are 
appropriately mitigated and provide a reasonable assurance of the 
safety and effectiveness of oncology therapeutic ISH-based test 
systems.
    (Comment 6) One comment acknowledged the suitability of the 
analytical performance tests outlined in the proposed order but 
recommended that FDA consider incorporating surrogate samples for use 
in the precision/reproducibility data generation, where the sample type 
can be supported as representative. This would apply to situations 
where sufficient clinical specimens for the intended use specimen 
type(s) are unavailable for analytical validation testing. The comment 
stated that such an inclusion would facilitate manufacturers' ability 
to conduct adequate verification testing by using surrogate samples 
that are morphologically comparable to the intended-use clinical 
specimens.
    (Response 6) Based on the information FDA considered and analyzed 
in proposing to reclassify these devices, as well as comments received 
in response to the proposed order, FDA acknowledges that certain 
exceptional clinical circumstances may warrant consideration of 
alternative approaches to the use of clinical specimens for the purpose 
of analytical validation testing (e.g., rare cancer(s) or tumor type(s) 
where obtaining sufficient clinical specimens presents a significant 
challenge). However, FDA maintains that precision studies represent a 
fundamental device performance requirement that is essential for 
demonstrating analytical performance and ensuring consistent and 
reliable device function. Upon further consideration, FDA has decided 
to modify the special control related to this comment (see Sec.  
864.1890(b)(1)(v)) to consider surrogate samples that adequately 
represent the intended use specimen type(s) and intended use 
biomarker(s), as appropriate, as determined by FDA, to supplement 
clinical specimens. For example, FDA will determine the appropriateness 
of surrogate samples based on clinical and scientific data and/or 
justification available to the Agency.
    In addition to the above modification, FDA, based in part on the 
comments received, has also removed the special control requiring 
device performance data demonstrating appropriate reagent stability. 
Upon further review of the information the Agency considered and 
analyzed in proposing to reclassify these devices, such as postmarket 
safety data from medical device reports and recalls, as well as 
comments received on the proposed special controls, FDA believes that a 
special control requiring reagent stability data is not necessary and 
that general controls, such as premarket notification (510(k)) 
requirements and quality system requirements set forth under part 820 
(21 CFR part 820) are sufficient to ensure that reagents are 
appropriately assessed and labeled such that this special control is 
not necessary. As such, the Agency is removing proposed Sec.  
864.1890(b)(1)(viii).
    (Comment 7) One comment recommended FDA revise the 
``Identification'' language of proposed Sec.  864.1890 to include the 
term ``companion.''
    (Response 7) FDA disagrees with the recommended edit and is 
finalizing the identification language in this final order without 
change. The comment did not provide context to support the suggested 
revision, and FDA continues to believe that the identification, as 
proposed, provides an appropriate level of clarity regarding the type 
of device intended to fall within the scope of Sec.  864.1890.
    (Comment 8) One comment expressed a desire to work with the Agency 
to address nuanced clinical performance adequacy scenarios as 
potentially applicable to future devices either via a pre-submission 
route or alternative mechanism.
    (Response 8) FDA agrees that questions of this nature may be 
appropriate topics to discuss with the Agency through the Q-Submission 
program. Prior to submission, a sponsor may seek FDA input on specific 
questions regarding review topics relevant to a planned marketing 
submission by utilizing our Q-Submission program. Through the Q-
Submission program FDA may provide input on device-specific 
requirements and recommendations intended to support a marketing 
submission. Additional information regarding the Q-Submission program 
can be found in FDA's final guidance document titled ``Requests for 
Feedback and Meetings for Medical Device Submissions: The Q-Submission 
Program'' (Ref. 1).
    Sponsors may also review information on FDA's Center for Devices 
and Radiological Health's (CDRH) website regarding previously approved 
oncology therapeutic ISH-based test systems, such as Summary of Safety 
and Effectiveness Data (SSED) documents available in FDA's Premarket 
Approval Database,\7\ which detail the clinical evidence, risks, and 
benefits for medical devices. Additionally, sponsors may consider 
reviewing CDRH's 510(k) Decision Summaries, found in FDA's 510(k) 
Premarket Notification Database,\8\ which summarize the information 
that informed the Agency's substantial

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equivalence decision and that may help inform interested parties 
regarding FDA's thinking on the types of performance the Agency might 
expect for this type of device. Sponsors also should refer to the 
special controls codified at Sec.  864.1890, established as part of 
this final order, which set forth requirements that are necessary to 
provide a reasonable assurance of safety and effectiveness for these 
devices. Comments related to a sponsor's study- or device-specific 
questions are outside the scope of this final order.
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    \7\ https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfpma/pma.cfm.
    \8\ https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfpmn/pmn.cfm.
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    (Comment 9) One comment requested that FDA evaluate other devices 
and therapies with comparable risk profiles to determine whether 
reclassification may be appropriate, with the goal of broadening 
availability of such devices. The comment also requested that FDA 
review and update the special controls for oncology therapeutic ISH-
based test systems on a regular basis and ensure that clinical data 
supporting the development and implementation of the special controls 
is transparent and readily available to interested parties.
    (Response 9) The FDA periodically reviews the classification of 
devices to ensure they are being regulated in the appropriate class 
(class III, II, I) with the necessary level of regulatory controls. 
CDRH has previously undertaken reclassification efforts as part of the 
Center's systematic approach, including the 2014-2015 Strategic 
Priorities,\9\ and as part of CDRH's regular due diligence in 
considering the specific classification for a particular device type. 
Additionally, in 2024, CDRH announced its intent to initiate the 
reclassification process for most high risk IVD devices, reflecting 
this ongoing, systematic approach to ensuring appropriate 
classification.\10\ FDA intends to continue evaluating the 
classification of devices to ensure they are subject to the appropriate 
level of regulatory controls.
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    \9\ See 81 FR 52445.
    \10\ See https://www.fda.gov/medical-devices/medical-devices-news-and-events/cdrh-announces-intent-initiate-reclassification-process-most-high-risk-ivds.
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    Further, FDA acknowledges the comment's request that the Agency 
regularly review and update the special controls and ensure 
transparency. For class II devices, special controls are established to 
provide reasonable assurance of safety and effectiveness for the device 
type and are developed based on the totality of available scientific 
evidence at the time of classification or reclassification. As new 
information becomes available, including advances in scientific 
knowledge and changes in device technology, FDA may evaluate whether 
such developments and device modifications give rise to new benefit-
risk considerations such that new or different special controls, in 
addition to general controls, are needed to assure the safety and 
effectiveness for the device type.
    With respect to transparency, FDA strives to make publicly 
available the scientific basis for its regulatory decisions, consistent 
with applicable statutes and regulations. In general, the clinical and 
scientific information that forms the basis of the special controls is 
included in the public docket associated with the classification or 
reclassification action, to the extent permitted by law. FDA, 
therefore, believes that the current regulatory framework provides an 
appropriate mechanism for ensuring the relevance of special controls 
and the availability of supporting information. No changes to the final 
order have been made in response to this comment.
    (Comment 10) One comment proposed edits to Table 1. Risks to Health 
and Mitigation Measures in the proposed order. The comment proposed 
additional mitigation measures to each of the three identified risks to 
health to include, for example, the addition of ``Device Description 
and Principle of Operation'' and ``Implementation of Controls, 
procedures and user training requirements.''
    (Response 10) As discussed in response to comment 5, FDA disagrees 
that such line edits or clarifications are needed to the mitigations 
identified in the proposed order. The mitigation measures proposed to 
be added to table 1 correspond to this commenter's proposed additions 
to the codified, as described in comment 5. As noted in response to 
comment 5, the language proposed by the commenter is generally already 
covered for oncology therapeutic ISH-based test systems by the 
regulatory requirements for IVDs under part 801 and Sec.  809.10, as 
well as the Agency's proposed special controls. FDA believes that the 
special controls set forth in the proposed order with the changes 
identified in this final order (see comment 5 and the Agency's 
response), together with general controls, are sufficient to 
effectively mitigate the risks to health identified in section V of the 
proposed order and are necessary to provide a reasonable assurance of 
the safety and effectiveness of oncology therapeutic ISH-based test 
systems. For these reasons, we decline to incorporate the proposed 
edits.
    With regards to the commenter's recommendation to include 
``Implementation of Controls, procedures and user training 
requirements'' in table 1 of the proposed order, FDA also disagrees 
that this is necessary. FDA believes that the commenter's proposed 
requirement is already encompassed within the Agency's proposed special 
controls. Specifically, this proposed recommendation falls within 
certain design verification and validation activities required by the 
special controls under Sec.  864.1890(b)(1)(i) which indicates that 
``[s]pecification for risk mitigation elements intended to mitigate 
risks associated with testing and results interpretation, including 
controls, procedures, and user training requirements, as appropriate.''
    (Comment 11) One comment requested clarification on how FDA's 
determination that devices under the relevant oncology therapeutic ISH-
based test system product codes share similar purposes, designs, 
functions, and risk profiles would affect their use as predicate 
devices under the 510(k) pathway. The commenter also sought general 
guidance on how substantial equivalence would be evaluated in cases 
where a device's intended use or labeling is expanded, such as to 
include a new oncology therapeutic product, clinical indication, or 
clinical cut-off.
    (Response 11) FDA acknowledges the commenter's request for 
clarification regarding the implications of this reclassification on 
the use of these devices as predicates under the 510(k) pathway. While 
the Agency has determined that devices within the relevant product 
codes share similar purposes, designs, functions, and overall risk 
profiles for purposes of reclassification, this determination does not 
alter the statutory and regulatory requirements applicable to 
demonstrating substantial equivalence. For a new device to be 
considered substantially equivalent to a predicate device, the new 
device must have the same intended use as the predicate device and the 
same technological characteristics or different technological 
characteristics that do not raise different questions of safety and 
effectiveness than the predicate device. Additional information can be 
found in FDA's final guidance ``The 510(k) Program: Evaluating 
Substantial Equivalence in Premarket Notifications [510(k)]'' (Ref. 4).
    In accordance with section 513(i) of the FD&C Act and part 807, 
substantial equivalence determinations are made on a case-by-case basis 
and depend on the specific intended use and technological 
characteristics of the device under review. Any oncology therapeutic 
ISH-based test system with a new intended use or technological 
characteristics that raise different questions of safety and

[[Page 53189]]

effectiveness compared to legally marketed devices would generally not 
be found to be substantially equivalent. However, devices could be used 
as predicate devices to support a substantial equivalence determination 
when a device has a new clinical cut-off or a new indication for use 
that includes a new therapeutic product if adequate data and 
information are provided to demonstrate the new indication(s) for use 
fall within the intended use of the predicate device and any 
technological differences do not raise different questions of safety 
and effectiveness. If a manufacturer has a question regarding a 
specific device for which they intend to seek marketing authorization, 
the manufacturer may choose to utilize the Q-Submission Program to seek 
feedback on the appropriate regulatory pathway for modified devices. 
Additional information regarding the Q-Submission program can be found 
in FDA's final guidance document titled ``Requests for Feedback and 
Meetings for Medical Device Submissions: The Q-Submission Program'' 
(Ref. 1).
    (Comment 12) One comment requested that the Agency clarify that 
devices already on the market would be automatically reclassified into 
class II without the need for any additional premarket notification or 
submission, and that only future devices, or currently marketed devices 
with significant modifications, would be required to submit a premarket 
notification.
    (Response 12) FDA appreciates the need to provide clarification on 
the implementation of this final order. To provide such clarification 
and assist in the efficient implementation of this final order, the 
Agency has added an implementation strategy in section IV of this final 
order. Among other things, section IV clarifies that upon its effective 
date the final order reclassifies devices that are oncology therapeutic 
ISH-based test systems as described within the scope of the proposed 
order and adopted as part of this final order, from class III 
(premarket approval) into class II (special controls). Oncology 
therapeutic ISH-based test systems with prior PMA approval may continue 
to be marketed per the previous marketing authorization and would not 
require an additional marketing application. For changes or 
modifications that could significantly affect the safety or 
effectiveness of such devices or a major change or modification in the 
intended use of such devices (see Sec.  807.81(a)(3)), FDA expects that 
manufacturers will submit a 510(k) for the modified device.
    (Comment 13) One comment encouraged FDA to issue a detailed 
guidance regarding the special controls described in the proposed order 
at the same time as the publication of this final order in the Federal 
Register.
    (Response 13) At this time, FDA does not intend to issue a guidance 
document regarding compliance with the special controls identified in 
this final order. For the reasons discussed in the proposed order, the 
Agency believes that the special controls, as stated in Sec.  
864.1890(b), provide sufficiently clear and appropriate requirements, 
at a level of detail necessary to reasonably assure the safety and 
effectiveness of this device type. Should FDA determine in the future 
that further information is warranted, the Agency may consider issuing 
guidance. The Agency also believes information available to sponsors 
through other resources will be helpful in preparing 510(k)s for 
submission and complying with special controls. For example, sponsors 
may review information on CDRH's website regarding previously approved 
oncology therapeutic ISH-based test systems such as SSED documents 
available in FDA's Premarket Approval Database, which detail the 
clinical evidence, risks, and benefits for approved devices. In 
addition, the performance data and related valid scientific evidence 
included in 510(k)s reviewed by FDA may be a helpful resource. Once 
available, sponsors may consider reviewing the 510(k) Decision 
Summaries in FDA's 510(k) Premarket Notification Database to inform the 
types of performance the Agency expects for this type of device.

III. The Final Order

    In this final order, FDA is adopting relevant findings, including 
the reasoning that supports those findings, from the June 11, 2025, 
proposed order. FDA has made revisions in this final order based, in 
part, on the comments received (see section II). FDA is issuing this 
final order to reclassify oncology therapeutic ISH-based test systems 
from class III into class II under a new device classification 
regulation with the name In Situ Hybridization Test Systems for Use 
with a Corresponding Approved Oncology Therapeutic Product, and to 
establish special controls by revising 21 CFR part 864 (adding Sec.  
864.1890).
    Further, in this final order, FDA has identified the special 
controls under section 513(a)(1)(B) of the FD&C Act that, along with 
general controls, provide a reasonable assurance of the safety and 
effectiveness for oncology therapeutic ISH-based test systems. As 
described in section II of this document, FDA has made revisions to the 
special controls as previously described in the proposed order. Based, 
in part, on comments regarding the proposed order, FDA has revised the 
design verification and validation special controls to add to the 
existing proposed Sec.  864.1890(b)(1)(v) requirement the consideration 
of surrogate samples that adequately represent the intended use 
specimen type(s) and intended use biomarker(s), as appropriate, as 
determined by FDA, to supplement clinical specimens. Additionally, FDA 
has removed the proposed Sec.  864.1890(b)(1)(viii) requiring device 
performance data demonstrating reagent stability.
    Based on the information discussed in the preambles to the proposed 
order and this final order, including the comments received for the 
proposed order, FDA concludes that special controls, in addition to 
general controls, provide a reasonable assurance of the safety and 
effectiveness of oncology therapeutic ISH-based test systems. In this 
final order, the Agency has identified the special controls under 
section 513(a)(1)(B) of the FD&C Act that, along with general controls, 
provide a reasonable assurance of the safety and effectiveness of these 
devices. In addition, in this final order, to provide additional 
clarification and to efficiently implement this order, the Agency has 
added an implementation strategy in section IV.
    Under the FD&C Act, 510(k) submissions are required to reasonably 
assure the safety and effectiveness of class II devices unless FDA 
determines that the device type should be exempt under section 
510(m).\11\ FDA has not made this determination for oncology 
therapeutic ISH-based test systems and, therefore, this class II device 
type is not exempt from 510(k) requirements. Thus, under sections 
510(k) and 513(f) of the FD&C Act, persons who intend to market this 
device type must submit a 510(k) containing information on the

[[Page 53190]]

oncology therapeutic ISH-based test system that they intend to market 
and must obtain FDA clearance of the device prior to marketing it.
---------------------------------------------------------------------------

    \11\ In considering whether to exempt class II devices from 
premarket notification, FDA considers whether premarket notification 
for the type of device is necessary to provide reasonable assurance 
of safety and effectiveness of the device. FDA generally considers 
the factors initially identified in 63 FR 3142 (January 21, 1998) 
and further explained in FDA's guidance ``Procedures for Class II 
Device Exemptions from Premarket Notification, Guidance for Industry 
and CDRH Staff,'' available at https://www.fda.gov/regulatory-information/search-fda-guidance-documents/procedures-class-ii-device-exemptions-premarket-notification-guidance-industry-and-cdrh-staff, to determine whether premarket notification is necessary for 
class II devices. FDA also considers that even when exempting 
devices from the 510(k) requirements, these devices would still be 
subject to certain limitations on exemptions, for example, the 
general limitations set forth in 21 CFR 864.9.
---------------------------------------------------------------------------

    Under this final order, oncology therapeutic ISH-based test systems 
are prescription use IVD devices and as such, these tests must satisfy 
prescription labeling requirements for IVD products (see Sec.  
809.10(a)(4) and (b)(5)(ii)). This device type is subject to the 
submission and device clearance requirements of sections 510(k) and 513 
of the FD&C Act and of part 807, subpart E, of FDA's regulations.

IV. Implementation Strategy

    This final order is effective 30 days after the date of its 
publication in the Federal Register.
    For oncology therapeutic ISH-based test systems that have not been 
offered for sale prior to the effective date of the final order, 
manufacturers must obtain 510(k) clearance before marketing the device 
(and for subsequent modifications to the device, as appropriate).
    For oncology therapeutic ISH-based test systems that have been 
offered for sale prior to the effective date of the final order and 
have prior PMA approval, such devices may continue to be marketed under 
the previously issued PMA approval and do not require an additional 
marketing authorization.
    For devices that have been legally marketed via PMA before the 
effective date of this final order, FDA expects that manufacturers will 
submit a 510(k) premarket notification when making a change or 
modification that could significantly affect the safety or 
effectiveness of the device or a major change or modification in the 
intended use of the device. See Sec.  807.81(a)(3).

V. Analysis of Environmental Impact

    The Agency has determined under 21 CFR 25.34(b) that this action is 
of a type that does not normally have a significant effect on the human 
environment. Therefore, neither an environmental assessment nor an 
environmental impact statement is required.

VI. Paperwork Reduction Act of 1995

    This final administrative order refers to previously approved 
collections of information found in FDA regulations. The previously 
approved collections of information are subject to review by the Office 
of Management and Budget (OMB) under the Paperwork Reduction Act of 
1995 (44 U.S.C. 3501-3521). The collections of information in part 820 
(Quality Management System Regulation) have been approved under OMB 
control number 0910-0073; the collections of information in 21 CFR part 
812 (Investigational Device Exemptions) have been approved under OMB 
control number 0910-0078; the collections of information in 21 CFR part 
814, subparts A through E (Premarket Approval of Medical Devices) have 
been approved under OMB control number 0910-0231; the collections of 
information in part 807, subpart E (Premarket Notification Procedures) 
have been approved under OMB control number 0910-0120; and the 
collections of information in parts 801 and 809 (Device Labeling) have 
been approved under OMB control number 0910-0485.

VII. Codification of Orders

    Under section 513(f)(3) of the FD&C Act, FDA may issue final orders 
to reclassify devices. FDA will continue to codify classifications and 
reclassifications in the Code of Federal Regulations (CFR). Changes 
resulting from final orders will appear in the CFR as newly codified 
orders. Therefore, under section 513(f)(3) of the FD&C Act, we are 
codifying in this final order the classification of In Situ 
Hybridization Test Systems for Use with a Corresponding Approved 
Oncology Therapeutic Product in the new Sec.  864.1890, under which 
these oncology therapeutic ISH-based test systems are reclassified from 
class III into class II.

VIII. References

    The following references are on display at the Dockets Management 
Staff (HFA-305), Food and Drug Administration, 5630 Fishers Lane, Rm. 
1061, Rockville, MD 20852, 240-402-7500, and are available for viewing 
by interested persons between 9 a.m. and 4 p.m., Monday through Friday; 
they are also available electronically at https://www.regulations.gov. 
Although FDA verified the website addresses in this document, please 
note that websites are subject to change over time.

1. FDA, ``Requests for Feedback and Meetings for Medical Device 
Submissions: The Q-Submission Program; Guidance for Industry and 
Food and Drug Administration Staff,'' May 29, 2025. (Available at 
https://www.fda.gov/regulatory-information/search-fda-guidance-documents/requests-feedback-and-meetings-medical-device-submissions-q-submission-program.)
2. FDA, ``Marketing Submission Recommendations for a Predetermined 
Change Control Plan for Artificial Intelligence-Enabled Device 
Software Functions; Guidance for Industry and Food and Drug 
Administration Staff,'' August 18, 2025. (Available at https://www.fda.gov/regulatory-information/search-fda-guidance-documents/marketing-submission-recommendations-predetermined-change-control-plan-artificial-intelligence.)
3. FDA, ``Replacement Reagent and Instrument Family Policy for In 
Vitro Diagnostic Devices; Guidance for Industry and Food and Drug 
Administration Staff,'' August 17, 2022. (Available at https://www.fda.gov/regulatory-information/search-fda-guidance-documents/replacement-reagent-and-instrument-family-policy-in-vitro-diagnostic-devices.)
4. FDA, ``The 510(k) Program: Evaluating Substantial Equivalence in 
Premarket Notifications [510(k)]; Guidance for Industry and Food and 
Drug Administration Staff,'' July 28, 2014. (Available at https://www.fda.gov/regulatory-information/search-fda-guidance-documents/510k-program-evaluating-substantial-equivalence-premarket-notifications-510k.)

List of Subjects in 21 CFR Part 864

    Blood, Medical devices, Packaging and containers.

    Therefore, under the Federal Food, Drug, and Cosmetic Act (21 
U.S.C. 321 et seq., as amended), and under authority delegated to the 
Commissioner of Food and Drugs, 21 CFR part 864 is amended as follows:

PART 864--HEMATOLOGY AND PATHOLOGY DEVICES

0
1. The authority citation for part 864 continues to read as follows:

    Authority:  21 U.S.C. 351, 360, 360c, 360e, 360j, 360l, 371.


0
2. Add Sec.  864.1890 to subpart B to read as follows:


Sec.  864.1890  In situ hybridization test systems for use with a 
corresponding approved oncology therapeutic product.

    (a) Identification. In situ hybridization (ISH) test systems 
indicated for use with a corresponding approved oncology therapeutic 
product are identified as prescription in vitro diagnostic devices 
consisting of nucleic acid probes intended for the qualitative or 
quantitative detection of specific nucleic acid sequences in human 
clinical specimens to provide information related to the use of a 
corresponding approved oncology therapeutic product as described in the 
corresponding approved oncology therapeutic product labeling.
    (b) Classification. Class II (special controls). The special 
controls for this device are:
    (1) Design verification and validation must include:
    (i) Specification for risk mitigation elements intended to mitigate 
risks

[[Page 53191]]

associated with testing and results interpretation, including controls, 
procedures, and user training requirements, as appropriate.
    (ii) Specification of the criteria for test result interpretation 
and reporting, including device cut-off(s) (i.e., clinical threshold(s) 
or the medical decision point(s) between positive and negative results) 
or other relevant criteria that distinguishes positive and negative or 
quantitative results. This information must include the rationale for 
the chosen cut-off(s) to include the upper reference of normal, or 
other relevant criteria and results supporting validation of the cut-
off(s) evaluating borderline samples around the clinical threshold(s). 
Scoring criteria for all applicable signals must be included.
    (iii) Device performance data demonstrating appropriate analytical 
sensitivity from studies using interphase nuclei from intended use 
specimen type(s) that are considered karyotypically normal, or through 
an alternative approach, as determined to be appropriate by FDA (e.g., 
probe sensitivity and probe limits).
    (iv) Device performance data demonstrating appropriate analytical 
specificity of the device for the intended use specimen type(s), as 
determined to be appropriate by FDA (e.g., probe specificity, 
interference study, cross-reactivity and cross contamination testing).
    (v) Device performance data demonstrating appropriate precision and 
reproducibility of the device using clinical specimens representing the 
intended use specimen type(s) and intended use biomarker(s) from the 
intended use population and investigating major sources of variability 
(e.g., multiple reagent lots, operators, instruments over multiple 
days, and inter- and intra-reader precision). Surrogate samples that 
adequately represent the intended use specimen type(s) and intended use 
biomarker(s) may be appropriate, as determined by FDA, to supplement 
clinical specimens. If the device will be used at more than one site, 
data must demonstrate adequate reproducibility across multiple intended 
use sites. Additionally, precision and reproducibility of the device 
must be evaluated with specimens near the clinical decision 
threshold(s) and near the limits of reportable range. Additionally, 
device performance data demonstrating appropriate precision must be 
included from studies evaluating the different signals and associated 
cut-offs and controls, as determined to be appropriate by FDA. 
Furthermore, precision of the device must be evaluated per specimen and 
in aggregate.
    (vi) Device performance data demonstrating appropriate device 
robustness, as determined to be appropriate by FDA. The study must 
assess the tolerance ranges for various critical test and specimen 
parameters, as applicable.
    (vii) Device performance data demonstrating linearity of 
quantitative results using samples covering the device measuring range, 
as applicable.
    (viii) Device performance data demonstrating appropriate specimen 
stability based on the intended use specimen type(s) of the device, as 
applicable.
    (ix) Clinical data generated using well-characterized clinical 
specimens representative of the intended use population demonstrating 
appropriate clinical performance of the device for its intended use, as 
determined to be appropriate by FDA.
    (2) Labeling must include:
    (i) An appropriate summary, as determined by FDA, of the 
performance studies performed and the results of those studies, 
including those that relate to all design verification and validation 
special controls.
    (ii) A limiting statement, as appropriate, that explains that the 
test results are intended to be interpreted by a qualified or 
appropriately trained reader in conjunction with other diagnostic 
laboratory test results and/or pathology test results, relevant 
clinical information, and proper controls.
    (iii) Language indicating that the test system is indicated for use 
with a corresponding FDA-approved oncology therapeutic product and 
device labeling must be consistent with the information set forth in 
the corresponding FDA-approved oncology therapeutic product labeling.

Grace R. Graham,
Deputy Commissioner for Policy, Legislation, and International Affairs.
[FR Doc. 2026-16727 Filed 8-14-26; 8:45 am]
BILLING CODE 4164-01-P