[Federal Register Volume 90, Number 114 (Monday, June 16, 2025)]
[Rules and Regulations]
[Pages 25123-25134]
From the Federal Register Online via the Government Publishing Office [www.gpo.gov]
[FR Doc No: 2025-10797]



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Rules and Regulations
                                                Federal Register
________________________________________________________________________

This section of the FEDERAL REGISTER contains regulatory documents 
having general applicability and legal effect, most of which are keyed 
to and codified in the Code of Federal Regulations, which is published 
under 50 titles pursuant to 44 U.S.C. 1510.

The Code of Federal Regulations is sold by the Superintendent of Documents. 

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Federal Register / Vol. 90, No. 114 / Monday, June 16, 2025 / Rules 
and Regulations

[[Page 25123]]



DEPARTMENT OF AGRICULTURE

Animal and Plant Health Inspection Service

7 CFR Parts 330, 340, and 372

[Docket No. APHIS-2018-0034]
RIN 0579-AE47


Movement of Certain Genetically Engineered Organisms

AGENCY: Animal and Plant Health Inspection Service, Department of 
Agriculture (USDA).

ACTION: Final rule; technical amendments.

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SUMMARY: On December 2, 2024, a Federal court vacated the Animal and 
Plant Health Inspection Service (APHIS) final rule issued on May 18, 
2020 (the May 2020 final rule) that revised APHIS' regulations 
governing the movement of certain genetically modified organisms and 
was issued under the authority of the Plant Protection Act. APHIS is 
therefore amending the CFR to conform the CFR to the Federal Court's 
vacatur of that rule. The Court's vacatur was effective December 2, 
2024, and had the legal effect of vacating the May 2020 final rule in 
its entirety and restoring the legal effect of the pre-May 2020 
regulations. These technical conforming amendments revise the CFR to 
reflect the court's vacatur of the May 2020 final rule.

DATES: This rule is effective June 16, 2025. The Federal Court's 
vacatur of the rule amendments published on May 18, 2020, at 85 FR 
29790, was applicable as of December 2, 2024.

FOR FURTHER INFORMATION CONTACT: Alan Pearson, Biotechnology Regulatory 
Services, APHIS, USDA, 4700 River Road, Unit 78, Riverdale, MD 20737-
1236; (301) 851-3944; email: [email protected].

SUPPLEMENTARY INFORMATION: The Animal and Plant Health Inspection 
Service is adopting technical amendments to the following regulations: 
7 CFR 330.200(b) and (d), 7 CFR part 340, and 7 CFR 372.5(b)(7) and 
(c)(3) and (4).

Background

    The regulations at 7 CFR part 340 govern the introduction 
(importation, interstate movement, or release into the environment) of 
certain genetically engineered (GE) organisms. APHIS first issued these 
regulations in 1987 under the authority of the Federal Plant Pest Act 
of 1957 and the Plant Quarantine Act of 1912, two acts that were later 
subsumed into the Plant Protection Act (PPA, 7 U.S.C. 7701 et seq.) in 
2000, along with other provisions. Since 1987 and prior to 2020, APHIS 
amended the regulations six times, in 1988, 1990, 1993, 1994, 1997, and 
2005, to institute exemptions from the requirement for permits to 
conduct activities for certain microorganisms and Arabidopsis, to 
institute a notification process and petition procedure, and to exclude 
plants engineered to produce industrial compounds from the notification 
process. On May 18, 2020, APHIS issued a final rule revising 7 CFR part 
340 in its entirety (85 FR 29790-29838, Docket No. APHIS-2018-0034, 
referred to below as the May 2020 final rule). The rule also made 
harmonizing changes to 7 CFR part 330, which governs the movement 
(importation, interstate movement, and environmental release) of plant 
pests, and 7 CFR part 372, which contains APHIS' National Environmental 
Policy Act (43 U.S.C. 4321 et seq.) implementing regulations.

Litigation

    On July 26, 2021, the National Family Farm Coalition (``NFFC''), 
Center for Food Safety, and Pesticide Action Network North America 
Diversity (collectively, Plaintiffs) on behalf of themselves and their 
members filed a complaint in the United States District Court for the 
Northern District of California, challenging the May 2020 final rule. 
The complaint alleged that the May 2020 final rule violated the 
Administrative Procedure Act (5 U.S.C. 551-559, APA), among other 
statutes. The parties subsequently completed briefings on cross motions 
for summary judgment.

Vacatur

    On December 2, 2024, the United States District Court of the 
Northern District of California issued its decision. The Court held 
that APHIS had violated the APA in issuing the May 2020 final rule. In 
its order, the Court vacated the May 2020 final rule in its entirety, 
including revisions to 7 CFR parts 330, 340, and 372. (The vacatur is 
prospective, which means that Agency actions taken under the May 2020 
final rule, such as Regulatory Status Review responses, Confirmation 
Request responses, and active permits that USDA issued prior to 
December 2, 2024, remain valid.) As a result, pursuant to the Court's 
order, the pre-May 2020 regulations again became operative and took 
legal effect on December 2, 2024. APHIS provided notice to the 
regulated parties of this development on December 4, 2024. See APHIS 
Stakeholder Registry Email of December 4, 2024. The Court's decision is 
now final. Accordingly, this technical amendment conforms the CFR with 
the Court's vacatur by replacing the regulations in the now-vacated May 
2020 final rule with the text of the regulations that were in effect 
prior to the May 2020 final rule, and which are now operative and in 
legal effect.

Good Cause

    The APA generally requires an agency to publish notice of a 
proposed rulemaking in Federal Register and provide an opportunity for 
public comment. This requirement does not apply, however, if the agency 
``for good cause finds . . . that notice and public procedure are 
impracticable, unnecessary, or contrary to the public interest.'' (5 
U.S.C. 553(b)(B)) The technical amendments we are making to the CFR 
merely reflect the Court's vacatur of the May 2020 final rule and 
neither impose nor relieve regulatory requirements on any person. 
Rather, those regulatory requirements are imposed because of the 
Court's vacatur of the May 2020 final rule and the return to the pre-
May 2020 regulations that occurred by operation of law. See Paulsen v. 
Daniels, 413 F.3d 999, 1008 (9th Cir. 2005) (``The effect of 
invalidating an agency rule is to reinstate the rule previously in 
force.''); Nat'l Fam. Farm Coal. v. Vilsack, 758 F. Supp. 3d 1060, 1081 
(N.D. Cal. 2024) (``this . . . vacatur suffices to return the industry 
and GE-crop regulation to the status quo ante''). Insofar as public 
comment has no material bearing on the

[[Page 25124]]

reality of the vacatur, APHIS finds good cause that notice and public 
comment are unnecessary.
    For similar reasons, although the APA generally requires 
publication of a rule at least 30 days before its effective date, APHIS 
finds there to be good cause for the amendments to take effect upon 
publication of this document in the Federal Register.

List of Subjects

7 CFR Part 330

    Customs duties and inspection, Plant diseases and pests, 
Quarantine, Reporting and recordkeeping requirements, Transportation.

7 CFR Part 340

    Administrative practice and procedure, Packaging and containers, 
Plant diseases and pests, Reporting and recordkeeping requirements, 
Transportation.

7 CFR Part 372

    Environmental impact statements.

    Accordingly, we are amending 7 CFR parts 330, 340, and 372 as 
follows:

PART 330--FEDERAL PLANT PEST REGULATIONS; GENERAL; PLANT PESTS, 
BIOLOGICAL CONTROL ORGANISMS, AND ASSOCIATED ARTICLES; GARBAGE

0
1. The authority citation for part 330 continues to read as follows:

    Authority: 7 U.S.C. 1633, 7701-7772, 7781-7786, and 8301-8317; 
21 U.S.C. 136 and 136a; 31 U.S.C. 9701; 7 CFR 2.22, 2.80, and 371.3.


0
2. In Sec.  330.200, paragraphs (b) and (d) are revised to read as 
follows:


Sec.  330.200  Scope and general restrictions.

* * * * *
    (b) Plant pests regulated by this subpart. For the purposes of this 
subpart, APHIS will consider an organism to be a plant pest if the 
organism directly or indirectly injures, causes damage to, or causes 
disease in a plant or plant product, or if the organism is an unknown 
risk to plants or plant products, but is similar to an organism known 
to directly or indirectly injure, cause damage to, or cause disease in 
a plant or plant product.
* * * * *
    (d) Biological control organisms not regulated by this subpart. 
Paragraph (c) of this section notwithstanding, biological control 
organism-containing products that are currently under an EPA 
experimental use permit, a Federal Insecticide Fungicide and 
Rodenticide Act (FIFRA) section 18 emergency exemption, or that are 
currently registered with EPA as a microbial pesticide product, are not 
regulated under this subpart. Additionally, biological control 
organisms that are pesticides that are not registered with EPA, but are 
being transferred, sold, or distributed in accordance with EPA's 
regulations in 40 CFR 152.30, are not regulated under this subpart for 
their interstate movement or importation. However, an importer desiring 
to import a shipment of biological control organisms subject to FIFRA 
must submit to the EPA Administrator a Notice of Arrival of Pesticides 
and Devices as required by CBP regulations at 19 CFR 12.112. The 
Administrator will provide notification to the importer indicating the 
disposition to be made of shipment upon its entry into the customs 
territory of the United States.


0
3. Part 340 is revised to read as follows:

PART 340--INTRODUCTION OF ORGANISMS AND PRODUCTS ALTERED OR 
PRODUCED THROUGH GENETIC ENGINEERING WHICH ARE PLANT PESTS OR WHICH 
THERE IS REASON TO BELIEVE ARE PLANT PESTS

Sec.
340.0 Restrictions on the introduction of regulated articles.
340.1 Definitions.
340.2 Groups of organisms which are or contain plant pests and 
exemptions.
340.3 Notification for the introduction of certain regulated 
articles.
340.4 Permits for the introduction of a regulated article.
340.5 Petition to amend the list of organisms.
340.6 Petition for determination of nonregulated status.
340.7 Marking and identity.
340.8 Container requirements for the movement of regulated articles.
340.9 Costs and charges.

    Authority: 7 U.S.C. 7701-7772 and 7781-7786; 31 U.S.C. 9701; 7 
CFR 2.22, 2.80, and 371.3.


Sec.  340.0  Restrictions on the introduction of regulated articles.

    (a) No person shall introduce any regulated article unless the 
Administrator is:
    (1) Notified of the introduction in accordance with Sec.  340.3, or 
such introduction is authorized in accordance with Sec.  340.4, or such 
introduction is conditionally exempt from permit requirements under 
Sec.  340.2(b); and
    (2) Such introduction is in conformity with all other applicable 
restrictions in this part.\1\
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    \1\ Part 340 regulates, among other things, the introduction of 
organisms and products altered or produced through genetic 
engineering that are plant pests or are believed to be plant pests. 
The introduction into the United States of such articles may also be 
subject to other regulations promulgated under the Plant Protection 
Act (7 U.S.C. 7701-7772) and found in 7 CFR part 319, 330, and 360. 
For example, under regulations promulgated in ``Subpart H--Plants 
for Planting'' (7 CFR 319.37-5 of this chapter), a permit is 
required for the importation of certain classes of plants for 
planting whether such plants are genetically engineered or not. 
Accordingly, individuals should refer to those regulations before 
importing any plants for planting.
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    (b) Any regulated article introduced not in compliance with the 
requirements of this part shall be subject to the immediate application 
of such remedial measures or safeguards as an inspector determines 
necessary to prevent the introduction of such plant pests.\2\
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    \2\ An inspector may hold, seize, quarantine, treat, apply other 
remedial measures to, destroy, or otherwise dispose of plants, plant 
pests, or other articles in accordance with sections 411, 412, 421, 
and 434 of the Plant Protection Act (7 U.S.C. 7711, 7712, 7731, and 
7754).
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Sec.  340.1  Definitions.

    Terms used in the singular form in this part shall be construed as 
the plural, and vice versa, as the case may demand. The following terms 
shall be construed, respectively, to mean:
    Administrator. The Administrator of the Animal and Plant Health 
Inspection Service (APHIS) or any other employee of APHIS to whom 
authority has been or may be delegated to act in the Administrator's 
stead.
    Animal and Plant Health Inspection Service (APHIS). An agency of 
the United States Department of Agriculture.
    Antecedent organism. An organism that has already been the subject 
of a determination of nonregulated status by APHIS under Sec.  340.6, 
and that is used as a reference for comparison to the regulated article 
under consideration under these regulations.
    Courtesy permit. A written permit issued by the Administrator, in 
accordance with Sec.  340.4(h).
    Donor organism. The organism from which genetic material is 
obtained for transfer to the recipient organism.
    Environment. All the land, air, and water; and all living organisms 
in association with land, air and water.
    Expression vector. A cloning vector designed so that a coding 
sequence inserted at a particular site will be transcribed and 
translated into protein.
    Genetic engineering. The genetic modification of organisms by 
recombinant DNA techniques.

[[Page 25125]]

    Inspector. Any employee of the Animal and Plant Health Inspection 
Service, U.S. Department of Agriculture, or other person, authorized by 
the Administrator, in accordance with law to enforce the provisions of 
this part.
    Interstate. From any State into or through any other State.
    Introduce or introduction. To move into or through the United 
States, to release into the environment, to move interstate, or any 
attempt thereat.
    Move (moving, movement). To ship, offer for shipment, offer for 
entry, import, receive for transportation, carry, or otherwise 
transport or move, or allow to be moved into, through, or within the 
United States.
    Organism. Any active, infective, or dormant stage or life form of 
an entity characterized as living, including vertebrate and 
invertebrate animals, plants, bacteria, fungi, mycoplasmas, mycoplasma-
like organisms, as well as entities such as viroids, viruses, or any 
entity characterized as living, related to the foregoing.
    Permit. A written permit issued by the Administrator, for the 
introduction of a regulated article under conditions determined by the 
Administrator, not to present a risk of plant pest introduction.
    Person. Any individual, partnership, corporation, company, society, 
association, or other organized group.
    Plant. Any living stage or form of any member of the plant kingdom 
\3\ including, but not limited to, eukaryotic algae, mosses, club 
mosses, ferns, angiosperms, gymnosperms, and lichens (which contain 
algae) including any parts (e.g., pollen, seeds, cells, tubers, stems) 
thereof, and any cellular components (e.g., plasmids, ribosomes, etc.) 
thereof.
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    \3\ The taxonomic scheme for the plant kingdom is that found in 
Synopsis and Classification of Living Organisms by S.P. Parker, 
McGraw Hill (1984).
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    Plant pest. Any living stage (including active and dormant forms) 
of insects, mites, nematodes, slugs, snails, protozoa, or other 
invertebrate animals, bacteria, fungi, other parasitic plants or 
reproductive parts thereof; viruses; or any organisms similar to or 
allied with any of the foregoing; or any infectious agents or 
substances, which can directly or indirectly injure or cause disease or 
damage in or to any plants or parts thereof, or any processed, 
manufactured, or other products of plants.
    Product. Anything made by or from, or derived from an organism, 
living or dead.
    Recipient organism. The organism which receives genetic material 
from a donor organism.
    Regulated article. Any organism which has been altered or produced 
through genetic engineering, if the donor organism, recipient organism, 
or vector or vector agent belongs to any genera or taxa designated in 
Sec.  340.2 and meets the definition of plant pest, or is an 
unclassified organism and/or an organism whose classification is 
unknown, or any product which contains such an organism, or any other 
organism or product altered or produced through genetic engineering 
which the Administrator, determines is a plant pest or has reason to 
believe is a plant pest. Excluded are recipient microorganisms which 
are not plant pests and which have resulted from the addition of 
genetic material from a donor organism where the material is well 
characterized and contains only non-coding regulatory regions.
    Release into the environment. The use of a regulated article 
outside the constraints of physical confinement that are found in a 
laboratory, contained greenhouse, or a fermenter or other contained 
structure.
    Responsible person. The person who has control and will maintain 
control over the introduction of the regulated article and assure that 
all conditions contained in the permit and requirements in this part 
are complied with. A responsible person shall be a resident of the 
United States or designate an agent who is a resident of the United 
States.
    Secretary. The Secretary of Agriculture, or any other officer or 
employee of the Department of Agriculture to whom authority to act in 
his/her stead has been or may hereafter be delegated.
    Stably integrated. The cloned genetic material is contiguous with 
elements of the recipient genome and is replicated exclusively by 
mechanisms used by recipient genomic DNA.
    State. Any State, the District of Columbia, American Samoa, Guam, 
Northern Mariana Islands, Puerto Rico, the Virgin Islands of the United 
States, and any other Territories or Districts of the United States.
    State regulatory official. State official with responsibilities for 
plant health, or any other duly designated State official, in the State 
where the introduction is to take place.
    United States. All of the States.
    Vector or vector agent. Organisms or objects used to transfer 
genetic material from the donor organism to the recipient organism.
    Well-characterized and contains only non-coding regulatory regions 
(e.g., operators, promoters, origins of replication, terminators, and 
ribosome binding regions). The genetic material added to a 
microorganism in which the following can be documented about such 
genetic material: (a) The exact nucleotide base sequence of the 
regulatory region and any inserted flanking nucleotides; (b) The 
regulatory region and any inserted flanking nucleotides do not code for 
protein or peptide; and (c) The regulatory region solely controls the 
activity of other sequences that code for protein or peptide molecules 
or act as recognition sites for the initiation of nucleic acid or 
protein synthesis.


Sec.  340.2  Groups of organisms which are or contain plant pests and 
exemptions.

    (a) Groups of organisms which are or contain plant pests. The 
organisms that are or contain plant pests are included in the taxa or 
group of organisms contained in the following list. Within any 
taxonomic series included on the list, the lowest unit of 
classification actually listed is the taxon or group which may contain 
organisms which are regulated. Organisms belonging to all lower taxa 
contained within the group listed are included as organisms that may be 
or may contain plant pests, and are regulated if they meet the 
definition of plant pest in Sec.  340.1.\4\
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    \4\ Any organism belonging to any taxa contained within any 
listed genera or taxa is only considered to be a plant pest if the 
organism ``can directly or indirectly injure, or cause disease, or 
damage in any plants or parts thereof, or any processed, 
manufactured, or other products of plants.'' Thus a particular 
unlisted species within a listed genus would be deemed a plant pest 
for purposes of Sec.  340.2, if the scientific literature refers to 
the organism as a cause of direct or indirect injury, disease, or 
damage to any plants, plant parts or products of plants. (If there 
is any question concerning the plant pest status of an organism 
belonging to any listed genera or taxa, the person proposing to 
introduce the organism in question should consult with APHIS to 
determine if the organism is subject to regulation.)

    Note: Any genetically engineered organism composed of DNA or RNA 
sequences, organelles, plasmids, parts, copies, and/or analogs, of 
or from any of the groups of organisms listed below shall be deemed 
a regulated article if it also meets the definition of plant pest in 
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Sec.  340.1.

GROUP
VIROIDS

Superkingdom Prokaryotae

Kingdom Virus

All members of groups containing plant viruses, and all other plant and 
insect viruses

Kingdom Monera

DIVISION BACTERIA
Family Pseudomonadaceae
    Genus Pseudomonas

[[Page 25126]]

    Genus Xanthomonas
Family Rhizobiaceae
    Genus Rhizobium
    Genus Bradyrhizobium
    Genus Agrobacterium
    Genus Phyllobacterium
Family Enterobacteriaceae
    Genus Erwinia
Family Streptomycetaceae
    Genus Streptomyces
Family Actinomycetacease
    Genus Actinomyces
Coryneform Group
    Genus Clavibacter
    Genus Arthrobacter
    Genus Curtobacterium
    Genus Corynebacteria
Gram-negative phloem-limited bacteria associated with plant diseases
Gram-negative xylem-limited bacteria associated with plant diseases
And all other bacteria associated with plant or insect diseases
Rickettsiaceae
    Rickettgial-like organisms associated with insect diseases
Class Mollicutes
Order Mycoplasmatales
Family Spiroplasmataceae
    Genus Spiroplasma
Mycoplasma-like organisms associated with plant diseases
Mycoplasma-like organisms associated with insect diseases

Superkingdom Eukaryotae

Kingdom Plantae

Subkingdom Thallobionta

DIVISION CHLOROPHYTA
    Genus Cephaleuros
    Genus Rhodochytrium
    Genus Phyllosiphon
DIVISION MYXOMYCOTA
Class Plasmodiophoromycetes
DIVISION EUMYCOTA
Class Chytridiomycetes
Order Chytridiales
Class Oomycetes
Order Lagenidiales
Family Lagenidiaceae
Family Olpidiopsidaceae
Order Peronosporales
Family Albuginaceae
Family Peronosporaceae
Family Pythiaceae
Order Saprolegniales
Family Saprolegniaceae
Family Leptolegniellaceae
Class Zygomycetes
Order Mucorales
Family Choanephoraceae
Family Mucoraceae
Family Entomophthoraceae
Class Hemiascomycetes
Family Protomycetaceae
Family Taphrinaceae
Class Loculoascomycetes
Order Myriangiales
Family Elsinoeaceae
Family Myriangiaceae
Order Asterinales
Order Dothideales
Order Chaetothyriales
Order Hysteriales
Family Parmulariaceae
Family Phillipsiellaceae
Family Hysteriaceae
Order Pleosporales
Order Melanommatales
Class Plectomycetes
Order Eurotiales
Family Ophiostomataceae
Order Ascophaerales
Class Pyrenomycetes
Order Erysiphales
Order Meliolales
Order Xylariales
Order Diaporthales
Order Hypocreales
Order Clavicipitales
Class Discomycetes
Order Phacidiales
Order Helotiales
Family Ascocorticiceae
Family Hemiphacidiaceae
Family Dermataceae
Family Sclerotiniaceae
Order Cytarriales
Order Medeolariales
Order Pezziales
Family Sarcosomataceae
Family Sarcoscyphaceae
Class Teliomycetes
Class Phragmobasidiomycetes
Family Auriculariaceae
Family Ceratobasidiaceae
Class Hymenomycetes
Order Exobasidiales
Order Agaricales
Family Corticiaceae
Family Hymenochaetaceae
Family Echinodontiaceae
Family Fistulinaceae
Family Clavariaceae
Family Polyporaceae
Family Tricholomataceae
Class Hyphomycetes
Class Coelomycetes
And all other fungi associated with plant or insect diseases

Subkingdom Embryobionta

    Note: Organisms listed in the Code of Federal Regulations as 
noxious weeds are regulated under the Federal Noxious Weed Act.

DIVISION MAGNOLIOPHYTA
Family Balanophoraceae--parasitic species
Family Cuscutaceae--parasitic species
Family Hydnoraceae--parasitic species
Family Krameriaceae--parasitic species
Family Lauraceae--parasitic species
    Genus Cassytha
Family Lennoaceae--parasitic species
Family Loranthaceae--parasitic species
Family Myzodendraceae--parasitic species
Family Olacaceae--parasitic species
Family Orobanchaceae--parasitic species
Family Rafflesiaceae--parasitic species
Family Santalaceae--parasitic species
Family Scrophulariaceae--parasitic species
    Genus Alectra
    Genus Bartsia
    Genus Buchnera
    Genus Buttonia
    Genus Castilleja
    Genus Centranthera
    Genus Cordylanthus
    Genus Dasistoma
    Genus Euphrasia
    Genus Gerardia
    Genus Harveya
    Genus Hyobanche
    Genus Lathraea
    Genus Melampyrum
    Genus Melasma
    Genus Orthantha
    Genus Orthocarpus
    Genus Pedicularis
    Genus Rhamphicarpa
    Genus Rhinanthus
    Genus Schwalbea
    Genus Seymeria
    Genus Siphonostegia
    Genus Sopubia
    Genus Striga
    Genus Tozzia
Family Viscaceae--parasitic species

Kingdom Animalia

Subkingdom Protozoa

    Genus Phytomonas
And all Protozoa associated with insect diseases

Subkingdom Eumetazoa

PHYLUM NEMATA
Class Secernentea
Order Tylenchida
Family Anguinidae
Family Belonolaimidae
Family Caloosiidae
Family Criconematidae
Family Dolichodoridae
Family Fergusobiidae
Family Hemicycliophoridae

[[Page 25127]]

Family Heteroderidae
Family Hoplolaimidae
Family Meloidogynidae
Family Nacobbidae
Family Neotylenchidae
Family Nothotylenchidae
Family Paratylenchidae
Family Pratylenchidae
Family Tylenchidae
Family Tylenchulidae
Order Aphelenchida
Family Aphelenchoididae
Class Adenophorea
Order Dorylaimida
Family Longidoridae
Family Trichodoridae
PHYLUM MOLLUSCA
Class Gastropoda
Subclass Pulmonata
Order Basommatophora
    Superfamily Planorbacea
Order Stylommatophora
    Subfamily Strophocheilacea
Family Succineidae
Superfamily Achatinacae
Superfamily Arionacae
Superfamily Limacacea
Superfamily Helicacea
Order Systellommatophora
    Superfamily Veronicellacea
PHYLUM ARTHROPODA
Class Arachnida
Order Parasitiformes
    Suborder Mesostigmata
    Superfamily Ascoidea
    Superfamily Dermanyssoidea
Order Acariformes
    Suborder Prostigmata
    Superfamily Eriophyoidea
    Superfamily Tetranychoidea
    Superfamily Eupodoidea
    Superfamily Tydeoidea
    Superfamily Erythraenoidea
    Superfamily Trombidioidea
    Superfamily Hydryphantoidea
    Superfamily Tarsonemoidea
    Superfamily Pyemotoidea
Suborder Astigmata
    Superfamily Hemisarcoptoidea
    Superfamily Acaroidea
Class Diplopoda
Order Polydesmida
Class Insecta
Order Collembola
Family Sminthoridae
Order Isoptera
Order Thysanoptera
Order Orthoptera
Family Acrididae
Family Gryllidae
Family Gryllacrididae
Family Gryllotalpidae
Family Phasmatidae
Family Ronaleidae
Family Tettigoniidae
Family Tetrigidae
Order Hemiptera
Family Thaumastocoridae
Family Aradidae
    Superfamily Piesmatoidea
    Superfamily Lygaeoidea
    Superfamily Idiostoloidea
    Superfamily Coreoidea
    Superfamily Pentatomoidea
    Superfamily Pyrrhocoroidea
    Superfamily Tingoidea
    Superfamily Miroidea
Order Homoptera
Order Coleoptera
Family Anobiidae
Family Apionidae
Family Anthribidae
Family Bostrichidae
Family Brentidae
Family Bruchidae
Family Buprestidae
Family Byturidae
Family Cantharidae
Family Carabidae
Family Cerambycidae
Family Chrysomelidae
Family Coccinellidae
    Subfamily Epilachninae
Family Curculionidae
Family Dermestidae
Family Elateridae
Family Hydrophilidae
    Genus Helophorus
Family Lyctidae
Family Meloidae
Family Mordellidae
Family Platypodidae
Family Scarabaeidae
    Subfamily Melolonthinae
    Subfamily Rutelinae
    Subfamily Cetoniinae
    Subfamily Dynastinae
Family Scolytidae
Family Selbytidae
Family Tenebrionidae
Order Lepidoptera
Order Diptera
Family Agromyzidae
Family Anthomyiidae
Family Cecidomyiidae
Family Chloropidae
Family Ephydridae
Family Lonchaeidae
Family Muscidae
    Genus Atherigona
Family Otitidae
    Genus Euxeta
Family Syrphidae
Family Tephritidae
Family Tipulidae
Order Hymenoptera
Family Apidae
Family Caphidae
Family Chalcidae
Family Cynipidae
Family Eurytomidae
Family Formicidae
Family Psilidae
Family Siricidae
Family Tenthredinidae
Family Torymidae
Family Xylocopidae
Unclassified organisms and/or organisms whose classification is 
unknown.

    (b) Exemptions. (1) A limited permit for interstate movement shall 
not be required for genetic material from any plant pest contained in 
Escherichia coli genotype K-12 (strain K-12 and its derivatives), 
sterile strains of Saccharomyces cerevisiae, or asporogenic strains of 
Bacillus subtilis, provided that all the following conditions are met:
    (i) The microorganisms are shipped in a container that meets the 
requirements of Sec.  340.8(b)(3);
    (ii) The cloned genetic material is maintained on a nonconjugation 
proficient plasmid and the host does not contain other conjugation 
proficient plasmids or generalized transducing phages;
    (iii) The cloned material does not include the complete infectious 
genome of a known plant pest;
    (iv) The cloned genes are not carried on an expression vector if 
the cloned genes code for:
    (A) A toxin to plants or plant products, or a toxin to organisms 
beneficial to plants; or
    (B) Other factors directly involved in eliciting plant disease 
(i.e., cell wall degrading enzymes); or
    (C) Substances acting as, or inhibitory to, plant growth 
regulators.
    (2) A limited permit for interstate movement is not required for 
genetic material from any plant pest contained in the genome of the 
plant Arabiodopsis thaliana, provided that all of the following 
conditions are met:
    (i) The plants or plant materials are shipped in a container that 
meets the requirements of Sec.  340.8(b)(1), (2), and (3);
    (ii) The cloned genetic material is stably integrated into the 
plant genome;
    (iii) The cloned material does not include the complete infectious 
genome of a known plant pest.


Sec.  340.3  Notification for the introduction of certain regulated 
articles.\5\
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    \5\ APHIS may issue guidelines regarding scientific procedures, 
practices, or protocols which it has found acceptable in making 
various determinations under the regulations. A person may follow an 
APHIS guideline or follow different procedures, practices, or 
protocols. When different procedures, practices, or protocols are 
followed, a person may, but is not required to, discuss the matter 
in advance with APHIS to help ensure that the procedures, practices, 
or protocols to be followed will be acceptable to APHIS.
---------------------------------------------------------------------------

    (a) General. Certain regulated articles may be introduced without a 
permit,

[[Page 25128]]

provided that the introduction is in compliance with the requirements 
of this section. Any other introduction of regulated articles require a 
permit under Sec.  340.4, with the exception of introductions that are 
conditionally exempt from permit requirements under Sec.  340.2(b) of 
this part.
    (b) Regulated articles eligible for introduction under the 
notification procedure. Regulated articles which meet all of the 
following six requirements and the performance standards set forth in 
paragraph (c) of this section are eligible for introduction under the 
notification procedure.
    (1) The regulated article is any plant species that is not listed 
as a noxious weed in regulations at 7 CFR part 360 under the Plant 
Protection Act (7 U.S.C. 7712), and, when being considered for release 
into the environment, the regulated article is not considered by the 
Administrator to be a weed in the area of release into the environment.
    (2) The introduced genetic material is ``stably integrated'' in the 
plant genome, as defined in Sec.  340.1.
    (3) The function of the introduced genetic material is known and 
its expression in the regulated article does not result in plant 
disease.
    (4) The introduced genetic material does not:
    (i) Cause the production of an infectious entity, or
    (ii) Encode substances that are known or likely to be toxic to 
nontarget organisms known or likely to feed or live on the plant 
species, or
    (iii) Encode products intended for pharmaceutical or industrial 
use.
    (5) To ensure that the introduced genetic sequences do not pose a 
significant risk of the creation of any new plant virus, plant virus-
derived sequences must be:
    (i) Noncoding regulatory sequences of known function, or
    (ii) Sense or antisense genetic constructs derived from viral genes 
from plant viruses that are prevalent and endemic in the area where the 
introduction will occur and that infect plants of the same host 
species, and that do not encode a functional noncapsid gene product 
responsible for cell-to-cell movement of the virus.
    (6) The plant has not been modified to contain the following 
genetic material from animal or human pathogens:
    (i) Any nucleic acid sequence derived from an animal or human 
virus, or
    (ii) Coding sequences whose products are known or likely causal 
agents of disease in animals or humans.
    (c) Performance standards for introductions under the notification 
procedure. The following performance standards must be met for any 
introductions under the notification procedure.
    (1) If the plants or plant materials are shipped, they must be 
shipped in such a way that the viable plant material is unlikely to be 
disseminated while in transit and must be maintained at the destination 
facility in such a way that there is no release into the environment.
    (2) When the introduction is an environmental release, the 
regulated article must be planted in such a way that they are not 
inadvertently mixed with non-regulated plant materials of any species 
which are not part of the environmental release.
    (3) The plants and plant parts must be maintained in such a way 
that the identity of all material is known while it is in use, and the 
plant parts must be contained or devitalized when no longer in use.
    (4) There must be no viable vector agent associated with the 
regulated article.
    (5) The field trial must be conducted such that:
    (i) The regulated article will not persist in the environment, and
    (ii) No offspring can be produced that could persist in the 
environment.
    (6) Upon termination of the field test:
    (i) No viable material shall remain which is likely to volunteer in 
subsequent seasons, or
    (ii) Volunteers shall be managed to prevent persistence in the 
environment.
    (d) Procedural requirements for notifying APHIS. The following 
procedures shall be followed for any introductions under the 
notification procedure:
    (1) Notification should be directed to the Animal and Plant Health 
Inspection Service, Plant Protection and Quarantine, Biotechnology and 
Scientific Services, Biotechnology Permits, 4700 River Road, Unit 147, 
Riverdale, Maryland 20737-1237.
    (2) The notification shall include the following:
    (i) Name, title, address, telephone number, and signature of the 
responsible person;
    (ii) Information necessary to identify the regulated article(s), 
including:
    (A) The scientific, common, or trade names, and phenotype of 
regulated article,
    (B) The designations for the genetic loci, the encoded proteins or 
functions, and donor organisms for all genes from which introduced 
genetic material was derived, and
    (C) The method by which the recipient was transformed;
    (iii) The names and locations of the origination and destination 
facilities for movement or the field site location for the 
environmental release; and the size of the introduction,
    (iv) The date and, in the case of environmental release, the 
expected duration of the introduction (release); and
    (v) A statement that certifies that introduction of the regulated 
article will be in accordance with the provisions of this section.
    (3) Notification must be submitted to APHIS:
    (i) At least 10 days prior to the day of introduction, if the 
introduction is interstate movement.
    (ii) At least 30 days prior to the day of introduction, if the 
introduction is an importation.
    (iii) At least 30 days prior to the day of introduction, if the 
introduction is an environmental release.
    (4) Field test reports must be submitted to APHIS within 6 months 
after termination of the field test. Field test reports shall include 
the APHIS reference number, methods of observation, resulting data, and 
analysis regarding all deleterious effects on plants, nontarget 
organisms, or the environment.
    (5) The Administrator, shall be notified of any unusual occurrence 
within the time periods and in the manner specified in Sec.  
340.4(f)(10).
    (6) Access shall be allowed for APHIS and State regulatory 
officials to inspect facilities and/or the field test site and any 
records necessary to evaluate compliance with the provisions of 
paragraphs (b) and (c) of this section.
    (e) Administrative action in response to notification. (1) APHIS 
will provide copies of all notifications to appropriate State 
regulatory official(s) for review within 5 business days of receipt. 
Comments to APHIS from appropriate State regulatory officials in 
response to notifications for interstate movement of regulated articles 
will not be required by APHIS prior to acknowledgment, although States 
may provide their reviews to APHIS at their discretion.
    (2) The Administrator, will provide acknowledgement within 10 days 
of receipt that the interstate movement is appropriate under 
notification.
    (3) The Administrator, will provide acknowledgement within 30 days 
of receipt that the importation is appropriate under notification.
    (4) APHIS will provide acknowledgment within 30 days of

[[Page 25129]]

receipt that the environmental release is appropriate under 
notification. Such acknowledgment will apply to field testing for 1 
year from the date of introduction, and may be renewed annually by 
submission of an additional notification to APHIS.
    (5) A person denied permission for introduction of a regulated 
article under notification may apply for a permit for introduction of 
that regulated article without prejudice.


Sec.  340.4  Permits for the introduction of a regulated 
article.6
---------------------------------------------------------------------------

    \6\ See footnote 5 in Sec.  340.3.
---------------------------------------------------------------------------

    (a) Application for permit. Two copies of a written application for 
a permit to introduce a regulated article, which may be obtained from 
APHIS, shall be submitted by the responsible person to the Animal and 
Plant Health Inspection Service, Plant Protection and Quarantine, 
Biotechnology and Scientific Services, Biotechnology Permits, 4700 
River Road, Unit 147, Riverdale, Maryland 20737-1237. If there are 
portions of the application deemed to contain trade secret or 
confidential business information (CBI), each page of the application 
containing such information should be marked ``CBI Copy''. In addition, 
those portions of the application which are deemed ``CBI'' shall be so 
designated. The second copy shall have all such CBI deleted and shall 
be marked on each page of the application where CBI was deleted, ``CBI 
Deleted''. If an application does not contain CBI then the first page 
of both copies shall be marked ``No CBI''.
    (b) Permit for release into the environment. An application for the 
release into the environment of a regulated article shall be submitted 
at least 120 days in advance of the proposed release into the 
environment. An initial review shall be completed by APHIS within 30 
days of the receipt of the application. If the application is complete, 
the responsible individual shall be notified of the date of receipt of 
the application for purposes of advising the applicant when the 120 day 
review period commenced.\7\ If the application is not complete, the 
responsible individual will be advised what additional information must 
be submitted. APHIS shall commence the 120 day review period upon 
receipt of the additional information, assuming the additional 
information submitted is adequate. When it is determined that an 
application is complete, APHIS shall submit to the State department of 
agriculture of the State where the release is planned, a copy of the 
initial review and a copy of the application marked, ``CBI Deleted'', 
or ``No CBI'' for State notification and review. The application shall 
include the following information: \8\
---------------------------------------------------------------------------

    \7\ The 120 day review period would be extended if preparation 
of an environmental impact statement in addition to an environmental 
assessment was necessary.
    \8\ Application forms are available without charge from the 
Animal and Plant Health Inspection Service, Plant Protection and 
Quarantine, Biotechnology and Scientific Services, Biotechnology 
Permits, 4700 River Road, Unit 147, Riverdale, Maryland 20737-1237, 
or from local offices which are listed in telephone directories. A 
person should specify in requesting the application that the permit 
is for the introduction of a regulated article subject to regulation 
under part 340.
---------------------------------------------------------------------------

    (1) Name, title, address, telephone number, signature of the 
responsible person and type of permit requested (for importation, 
interstate movement, or release into the environment);
    (2) All scientific, common, and trade names, and all designations 
necessary to identify the: Donor organism(s); recipient organism(s); 
vector or vector agent(s); constituent of each regulated article which 
is a product; and, regulated article;
    (3) Names, addresses, and telephone numbers of the persons who 
developed and/or supplied the regulated article;
    (4) A description of the means of movement (e.g., mail, common 
carrier, baggage, or handcarried (and by whom));
    (5) A description of the anticipated or actual expression of the 
altered genetic material in the regulated article and how that 
expression differs from the expression in the non-modified parental 
organism (e.g., morphological or structural characteristics, 
physiological activities and processes, number of copies of inserted 
genetic material and the physical state of this material inside the 
recipient organism (integrated or extrachromosomal), products and 
secretions, growth characteristics);
    (6) A detailed description of the molecular biology of the system 
(e.g., donor-recipient-vector) which is or will be used to produce the 
regulated article;
    (7) Country and locality where the donor organism, recipient 
organism, vector or vector agent, and regulated article were collected, 
developed, and produced;
    (8) A detailed description of the purpose for the introduction of 
the regulated article including a detailed description of the proposed 
experimental and/or production design;
    (9) The quantity of the regulated article to be introduced and 
proposed schedule and number of introductions;
    (10) A detailed description of the processes, procedures, and 
safeguards which have been used or will be used in the country of 
origin and in the United States to prevent contamination, release, and 
dissemination in the production of the: Donor organism; recipient 
organism; vector or vector agent; constituent of each regulated article 
which is a product; and regulated article;
    (11) A detailed description of the intended destination (including 
final and all intermediate destinations), uses, and/or distribution of 
the regulated article (e.g., greenhouses, laboratory, or growth chamber 
location; field trial location; pilot project location; production, 
propagation, and manufacture location; proposed sale and distribution 
location);
    (12) A detailed description of the proposed procedures, processes, 
and safeguards which will be used to prevent escape and dissemination 
of the regulated article at each of the intended destinations;
    (13) A detailed description of any biological material (e.g., 
culture medium, or host material) accompanying the regulated article 
during movement; and
    (14) A detailed description of the proposed method of final 
disposition of the regulated article.
    (c) Limited permits for interstate movement or importation of a 
regulated article. An application for the interstate movement or 
importation of a regulated article shall be submitted at least 60 days 
in advance of the first proposed interstate movement and at least 60 
days prior to each importation. An initial review shall be completed by 
APHIS within 15 days of the receipt of the application. If the 
application is complete, the responsible person shall be notified of 
the date of receipt of the application for purposes of advising the 
applicant when the 60 day review period commenced. If the application 
is not complete, the responsible person will be advised what additional 
information must be submitted. APHIS shall commence the 60 day review 
period upon receipt of the additional information, assuming the 
additional information submitted is adequate. When it is determined 
that an application is complete, APHIS shall submit to the State 
department of agriculture of the State of destination of the regulated 
article a copy of the initial review and the application marked, ``CBI 
Deleted'', or ``No CBI'' for State notification and review.
    (1) Limited permit for interstate movement. The responsible person 
may apply for a single limited permit for the interstate movement of 
multiple regulated articles in lieu of submitting an application for 
each individual

[[Page 25130]]

interstate movement. Each limited permit issued shall be numbered and 
shall be valid for one year from the date of issuance. If a permit is 
sought for multiple interstate movements between contained facilities 
the responsible individual shall specify in the permit application all 
the regulated articles to be moved interstate; the origins and 
destinations of all proposed shipments; a detailed description of all 
the contained facilities where regulated articles will be utilized at 
destination; and a description of the containers that will be used to 
transport the regulated articles. A limited permit for interstate 
movement of a regulated article shall only be valid for the movement of 
those regulated articles moving between those locations specified in 
the application. If a person seeks to move regulated articles other 
than those specified in the application, or to a location other than 
those listed in the application, a supplemental application shall be 
submitted to APHIS. No person shall move a regulated article interstate 
unless the number of the limited permit appears on the outside of the 
shipping container. The responsible person shipping a regulated article 
interstate shall keep records for one year demonstrating that the 
regulated article arrived at its intended destination. The responsible 
person seeking a limited permit for interstate movement shall submit on 
an application form obtained from APHIS, the data required by 
paragraphs (b)(1), (2), (4), (6), (7), (9), and (11) through (14) of 
this section.
    (2) Limited permit for importation. The responsible person seeking 
a permit for the importation of a regulated article shall submit an 
application for a permit prior to the importation of each shipment of 
regulated articles. The responsible person importing a regulated 
article shall keep records for one year demonstrating that the 
regulated article arrived at its intended destination. The responsible 
person seeking a limited permit for importation shall submit on an 
application form obtained from APHIS data required by paragraphs 
(b)(1), (2), (4), (6), (7), (9), and (11) through (14) of this 
section.\9\
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    \9\ Renewals may receive shorter review. In the case of a 
renewal for a limited permit for importation that has been issued 
less than one year earlier, APHIS will notify the responsible person 
within 15 days that either: (1) The renewal permit is approved or 
(2) that a 60 day review period is necessary because the conditions 
of the original permit have changed.
---------------------------------------------------------------------------

    (d) Premises inspection. An inspector may inspect the site or 
facility where regulated articles are proposed, pursuant to a permit, 
to be released into the environment or contained after their interstate 
movement or importation. Failure to allow the inspection of a premises 
prior to the issuance of a permit or limited permit shall be grounds 
for the denial of the permit.
    (e) Administrative action on applications. After receipt and review 
by APHIS of the application and the data submitted pursuant to 
paragraph (a) of this section, including any additional information 
requested by APHIS, a permit shall be granted or denied. If a permit is 
denied, the applicant shall be promptly informed of the reasons why the 
permit was denied and given the opportunity to appeal the denial in 
accordance with the provisions of paragraph (g) of this section. If a 
permit is granted, the permit will specify the applicable conditions 
for introduction of the regulated article under this part.
    (f) Permit conditions. A person who is issued a permit and his/her 
employees or agents shall comply with the following conditions, and any 
supplemental conditions which shall be listed on the permit, as deemed 
by the Administrator to be necessary to prevent the dissemination and 
establishment of plant pests:
    (1) The regulated article shall be maintained and disposed of (when 
necessary) in a manner so as to prevent the dissemination and 
establishment of plant pests.
    (2) All packing material, shipping containers, and any other 
material accompanying the regulated article shall be treated or 
disposed of in such a manner so as to prevent the dissemination and 
establishment of plant pests.
    (3) The regulated article shall be kept separate from other 
organisms, except as specifically allowed in the permit;
    (4) The regulated article shall be maintained only in areas and 
premises specified in the permit;
    (5) An inspector shall be allowed access, during regular business 
hours, to the place where the regulated article is located and to any 
records relating to the introduction of a regulated article;
    (6) The regulated article shall, when possible, be kept identified 
with a label showing the name of the regulated article, and the date of 
importation;
    (7) The regulated article shall be subject to the application of 
measures determined by the Administrator to be necessary to prevent the 
accidental or unauthorized release of the regulated article;
    (8) The regulated article shall be subject to the application of 
remedial measures (including disposal) determined by the Administrator 
to be necessary to prevent the spread of plant pests;
    (9) A person who has been issued a permit shall submit to APHIS a 
field test report within 6 months after the termination of the field 
test. A field test report shall include the APHIS reference number, 
methods of observation, resulting data, and analysis regarding all 
deleterious effects on plants, nontarget organisms, or the environment.
    (10) APHIS shall be notified within the time periods and manner 
specified below, in the event of the following occurrences:
    (i) Orally notified immediately upon discovery and notify in 
writing within 24 hours in the event of any accidental or unauthorized 
release of the regulated article;
    (ii) In writing as soon as possible but not later than within 5 
working days if the regulated article or associated host organism is 
found to have characteristics substantially different from those listed 
in the application for a permit or suffers any unusual occurrence 
(excessive mortality or morbidity, or unanticipated effect on non-
target organisms);
    (11) A permittee or his/her agent and any person who seeks to 
import a regulated article into the United States shall:
    (i) Import or offer the regulated article for entry only through 
any USDA plant inspection station listed in accordance with Sec.  
319.37-8(a) of this chapter;
    (ii) Notify APHIS promptly upon arrival of any regulated article at 
a port of entry, of its arrival by such means as a manifest, customs 
entry document, commercial invoice, waybill, a broker's document, or a 
notice form provided for such purpose; and
    (iii) Mark and identify the regulated article in accordance with 
Sec.  340.5 of this part.
    (g) Withdrawal or denial of a permit. Any permit which has been 
issued may be withdrawn by an inspector or the Administrator if he/she 
determines that the holder thereof has not complied with one or more of 
the conditions listed on the permit. APHIS will confirm the reasons for 
the withdrawal of the permit in writing within ten (10) days. Any 
person whose permit has been withdrawn or any person who has been 
denied a permit may appeal the decision in writing to the Administrator 
within ten (10) days after receiving the written notification of the 
withdrawal or denial. The appeal shall state all of the facts and 
reasons upon which the person relies to show that the permit was 
wrongfully withdrawn or denied. The Administrator shall grant or deny 
the appeal, in writing, stating the

[[Page 25131]]

reasons for the decision as promptly as circumstances allow. If there 
is a conflict as to any material fact, a hearing shall be held to 
resolve such conflict. Rules of practice concerning such a hearing will 
be adopted by the Administrator.
    (h) Courtesy permit--(1) Issuance. The Administrator may issue a 
courtesy permit for the introduction of organisms modified through 
genetic engineering which are not subject to regulation under this part 
to facilitate movement when the movement might otherwise be impeded 
because of the similarity of the organism to other organisms regulated 
under this part.
    (2) Application. A person seeking a courtesy permit shall submit on 
an application form obtained from APHIS data required by paragraphs 
(b)(1), (2), and (5) of this section and shall indicate such data is 
being submitted as a request for a courtesy permit. A person should 
also include a statement explaining why he or she believes the organism 
or product does not come within the definition of a regulated article. 
The application shall be submitted at least 60 days prior to the time 
the courtesy permit is sought.
    (3) Administrative action. APHIS shall complete an initial review 
within 15 days of the date of receipt of the application. If the 
application is complete, the responsible individual shall be notified 
of the date of receipt of the application for purposes of advising the 
applicant when the 60 day review period commenced. If the application 
is not complete, the responsible individual will be advised what 
additional information must be submitted, and shall commence the 60 day 
review period upon receipt of the additional information, assuming the 
additional information submitted is adequate. Within 60 days from the 
date of receipt of a complete application, APHIS will either issue a 
courtesy permit or advise the responsible individual that a permit is 
required under paragraph (b) or (c) of this section.


(Approved by the Office of Management and Budget under control number 
0579-0216)


Sec.  340.5  Petition to amend the list of organisms.10
---------------------------------------------------------------------------

    \10\ See footnote 5 in Sec.  340.3.
---------------------------------------------------------------------------

    (a) General. Any person may submit to the Administrator a petition 
to amend the list of organisms in Sec.  340.2 of this part by adding or 
deleting any genus, species, or subspecies. A petitioner may 
supplement, amend, or withdraw a petition in writing without prior 
approval of the Administrator and without prejudice to resubmission at 
any time until the Administrator rules on the petition. A petition to 
amend the list of organisms shall be submitted in accordance with the 
procedures and format specified by this section.
    (b) Submission procedures and format. A person shall submit two 
copies of a petition to the Animal and Plant Health Inspection Service, 
Biotechnology and Scientific Services, PPQ, Biotechnology Permits, 4700 
River Road, Unit 147, Riverdale, Maryland 20737-1237. The petition 
should be dated, and structured as follows:

Petition To Amend 7 CFR 340.2

    The undersigned submits this petition under 7 CFR 340.4 to request 
that the Administrator [add the following genus, species, or subspecies 
to the list of organisms in 7 CFR 340.2] or [to remove the following 
genus, species, or subspecies from the list of organisms in Sec.  
340.2].

A. Statement of Grounds

    (A person must present a full statement explaining the factual 
grounds why the genus, species, or subspecies to be added to Sec.  
340.2 of this part is a plant pest or why there is reason to believe 
the genus, species, or subspecies is a plant pest or why the genus, 
species, or subspecies sought to be removed is not a plant pest or why 
there is reason to believe the genus, species, or subspecies is not a 
plant pest. The petition should include copies of scientific literature 
which the petitioner is relying upon, copies of unpublished studies, or 
data from tests performed. The petition should not include trade secret 
or confidential business information.
    A person should also include representative information known to 
the petitioner which would be unfavorable to a petition for listing or 
delisting. (If a person is not aware of any unfavorable information the 
petition should state, Unfavorable Information: NONE).

B. Certification

    The undersigned certifies, that to the best knowledge and belief of 
the undersigned, this petition includes all information and views on 
which the petitioner relies, and that it includes representative data 
and information known to the petitioner which are unfavorable to the 
petition.


(Signature) ____________

(Name of petitioner) ________

(Mailing address) _________

(Telephone number) ________

    (c) Administrative action on a petition. (1) A petition to amend 
the list of organisms which meets the requirements of paragraph (b) of 
this section will be filed by the APHIS, stamped with the date of 
filing, and assigned a docket number. The docket number shall identify 
the file established for all submissions relating to the petition. 
APHIS, will promptly notify the petitioner in writing of the filing and 
docket number of a petition. If a petition does not meet the 
requirements of paragraph (b) of this section, the petitioner shall be 
sent a notice indicating how the petition is deficient.
    (2) After the filing of a petition to amend the list of organisms 
USDA shall publish a proposal in the Federal Register to amend Sec.  
340.2 and solicit comments thereon from the public. An interested 
person may submit written comments to the APHIS on a filed petition, 
which shall become part of the docket file.
    (3) The Administrator shall furnish a response to each petitioner 
within 180 days of receipt of the petition. The response will either:
    (i) Approve the petition in whole or in part in which case the 
Administrator shall concurrently take appropriate action (publication 
of a document in the Federal Register amending Sec.  340.2 of this 
part); or
    (ii) deny the petition in whole or in part. The petitioner shall be 
notified in writing of the Administrator's decision. The decision shall 
be placed in the public docket file in the offices of APHIS, and in the 
form of a notice published in the Federal Register.


Sec.  340.6  Petition for determination of nonregulated status.\11\
---------------------------------------------------------------------------

    \11\ See footnote 5 in Sec.  340.3.
---------------------------------------------------------------------------

    (a) General. Any person may submit to the Administrator, a petition 
to seek a determination that an article should not be regulated under 
this part. A petitioner may supplement, amend, or withdraw a petition 
in writing without prior approval of the Administrator, and without 
affecting resubmission at any time until the Administrator, rules on 
the petition. A petition for determination of nonregulated status shall 
be submitted in accordance with the procedure and format specified in 
this section.
    (b) Submission procedures and format. A person shall submit two 
copies of a petition to the Animal and Plant Health Inspection Service, 
Plant Protection and Quarantine, Biotechnology and Scientific Services, 
Biotechnology Coordination and Technical Assistance, 4700 River Road, 
Unit 146, Riverdale, Maryland 20737-

[[Page 25132]]

1237. The petition shall be dated and structured as follows:

Petition for Determination of Nonregulated Status

    The undersigned submits this petition under 7 CFR 340.6 to request 
that the Administrator, make a determination that the article should 
not be regulated under 7 CFR part 340.


Signature _____________

A. Statement of Grounds

    A person must present a full statement explaining the factual 
grounds why the organism should not be regulated under 7 CFR part 340. 
The petitioner shall include copies of scientific literature, copies of 
unpublished studies, when available, and data from tests performed upon 
which to base a determination. The petition shall include all 
information set forth in paragraph (c) of 7 CFR 340.6. If there are 
portions of the petition deemed to contain trade secret or confidential 
business information (CBI), each page of the petition containing such 
information should be marked ``CBI Copy''. In addition, those portions 
of the petition which are deemed ``CBI'' shall be so designated. The 
second copy shall have all such CBI deleted and shall have marked on 
each page where the CBI was deleted: ``CBI Deleted.'' If a petition 
does not contain CBI, the first page of both copies shall be marked: 
``No CBI.''
    A person shall also include information known to the petitioner 
which would be unfavorable to a petition. If a person is not aware of 
any unfavorable information, the petition should state, ``Unfavorable 
information: NONE.''

B. Certification

    The undersigned certifies, that to the best knowledge and belief of 
the undersigned, this petition includes all information and views on 
which to base a determination, and that it includes relevant data and 
information known to the petitioner which are unfavorable to the 
petition.


(Signature) ____________

(Name of petitioner) ________

(Mailing address) _________

(Telephone number) ________

    (c) Required data and information. The petition shall include the 
following information:
    (1) Description of the biology of the nonmodified recipient plant 
and information necessary to identify the recipient plant in the 
narrowest taxonomic grouping applicable.
    (2) Relevant experimental data and publications.
    (3) A detailed description of the differences in genotype between 
the regulated article and the nonmodified recipient organism. Include 
all scientific, common, or trade names, and all designations necessary 
to identify: the donor organism(s), the nature of the transformation 
system (vector or vector agent(s)), the inserted genetic material and 
its product(s), and the regulated article. Include country and locality 
where the donor, the recipient, and the vector organisms and the 
regulated articles are collected, developed, and produced.
    (4) A detailed description of the phenotype of the regulated 
article. Describe known and potential differences from the unmodified 
recipient organism that would substantiate that the regulated article 
is unlikely to pose a greater plant pest risk than the unmodified 
organism from which it was derived, including but not limited to: Plant 
pest risk characteristics, disease and pest susceptibilities, 
expression of the gene product, new enzymes, or changes to plant 
metabolism, weediness of the regulated article, impact on the weediness 
of any other plant with which it can interbreed, agricultural or 
cultivation practices, effects of the regulated article on nontarget 
organisms, indirect plant pest effects on other agricultural products, 
transfer of genetic information to organisms with which it cannot 
interbreed, and any other information which the Administrator believes 
to be relevant to a determination. Any information known to the 
petitioner that indicates that a regulated article may pose a greater 
plant pest risk than the unmodified recipient organism shall also be 
included.
    (5) Field test reports for all trials conducted under permit or 
notification procedures, involving the regulated article, that were 
submitted prior to submission of a petition for determination of 
nonregulated status or prior to submission of a request for extension 
of a determination of nonregulated status under paragraph (e) of this 
part. Field test reports shall include the APHIS reference number, 
methods of observation, resulting data, and analysis regarding all 
deleterious effects on plants, nontarget organisms, or the environment.
    (d) Administrative action on a petition. (1) A petition for 
determination of nonregulated status under this part which meets the 
requirements of paragraphs (b) and (c) of this section will be filed by 
the Administrator, stamped with the date of filing, and assigned a 
petition number. The petition number shall identify the file 
established for all submissions relating to the petition. APHIS will 
promptly notify the petitioner in writing of the filing and the 
assigned petition number. If a petition does not meet the requirements 
specified in this section, the petitioner shall be sent a notice 
indicating how the petition is deficient.
    (2) After the filing of a completed petition, APHIS shall publish a 
notice in the Federal Register. This notice shall specify that comments 
will be accepted from the public on the filed petition during a 60 day 
period commencing with the date of the notice. During the comment 
period, any interested person may submit to the Administrator, written 
comments, regarding the filed petition, which shall become part of the 
petition file.
    (3) The Administrator shall, based upon available information, 
furnish a response to each petitioner within 180 days of receipt of a 
completed petition. The response will either:
    (i) Approve the petition in whole or in part; or
    (ii) deny the petition.
    The petitioner shall be notified in writing of the Administrator's 
decision. The decision shall be placed in the public petition file in 
the offices of APHIS and notice of availability published in the 
Federal Register.
    (e) Extensions to determinations of nonregulated status. (1) The 
Administrator may determine that a regulated article does not pose a 
potential for plant pest risk, and should therefore not be regulated 
under this part, based on the similarity of that organism to an 
antecedent organism.
    (2) A person may request that APHIS extend a determination of 
nonregulated status to other organisms. Such a request shall include 
information to establish the similarity of the antecedent organism and 
the regulated articles in question.
    (3) APHIS will announce in the Federal Register all preliminary 
decisions to extend determinations of nonregulated status 30 days 
before the decisions become final and effective. If additional 
information becomes available that APHIS believes justifies changing 
its decision, it will issue a revised decision.
    (4) If a request to APHIS to extend a determination of nonregulated 
status under this part is denied, APHIS will inform the submitter of 
that request of the reasons for denial. The submitter may submit a 
modified request or a separate petition for determination of 
nonregulated status without prejudice.

[[Page 25133]]

    (f) Denial of a petition; appeal. (1) The Administrator's written 
notification of denial of a petition shall briefly set forth the reason 
for such denial. The written notification shall be sent by certified 
mail. Any person whose petition has been denied may appeal the 
determination in writing to the Administrator within 10 days from 
receipt of the written notification of denial.
    (2) The appeal shall state all of the facts and reasons upon which 
the person relies, including any new information, to show that the 
petition was wrongfully denied. The Administrator shall grant or deny 
the appeal, in writing, stating the reasons for the decision as 
promptly as circumstances allow. An informal hearing may be held by the 
Administrator if there is a dispute of a material fact. Rules of 
Practice concerning such a hearing will be adopted by the 
Administrator.


Sec.  340.7  Marking and identity.

    (a) Any regulated article to be imported other than by mail, shall, 
at the time of importation into the United States, plainly and 
correctly bear on the outer container the following information:
    (1) General nature and quantity of the contents;
    (2) Country and locality where collected, developed, manufactured, 
reared, cultivated or cultured;
    (3) Name and address of shipper, owner, or person shipping or 
forwarding the organism;
    (4) Name, address, and telephone number of consignee;
    (5) Identifying shipper's mark and number; and
    (6) Number of written permit authorizing the importation.
    (b) Any regulated article imported by mail, shall be plainly and 
correctly addressed and mailed to APHIS through any USDA plant 
inspection station listed in accordance with Sec.  319.37-8(a) of this 
chapter and shall be accompanied by a separate sheet of paper within 
the package plainly and correctly bearing the name, address, and 
telephone number of the intended recipient, and shall plainly and 
correctly bear on the outer container the following information:
    (1) General nature and quantity of the contents;
    (2) Country and locality where collected, developed, manufactured, 
reared, cultivated, or cured;
    (3) Name and address of shipper, owner, or person shipping or 
forwarding the regulated article; and
    (4) Number of permit authorizing the importation.
    (c) Any regulated article imported into the United States by mail 
or otherwise shall, at the time of importation or offer for importation 
into the United States, be accompanied by an invoice or packing list 
indicating the contents of the shipment.


Sec.  340.8  Container requirements for the movement of regulated 
articles.

    (a) General requirements. A regulated article shall not be moved 
unless it complies with the provisions of paragraph (b) of this 
section, unless a variance has been granted in accordance with the 
provisions of paragraph (c) of this section.\12\
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    \12\ The requirements of this section are in addition to and not 
in lieu of any other packing requirements such as those for the 
transportation of etiologic agents prescribed by the Department of 
Transportation in Title 49 CFR or any other agency of the Federal 
government.
---------------------------------------------------------------------------

    (b) Container requirements--(1) Plants and plant parts. All plants 
or plant parts, except seeds, cells, and subcellular elements, shall be 
packed in a sealed plastic bag of at least 5 mil thickness, inside a 
sturdy, sealed, leak-proof, outer shipping container constructed of 
corrugated fiberboard, corrogated cardboard, wood, or other material of 
equivalent strength.
    (2) Seeds. All seeds shall be transported in a sealed plastic bag 
of at least 5 mil thickness, inside a sealed metal container, which 
shall be placed inside a second sealed metal container. Shock absorbing 
cushioning material shall be placed between the inner and outer metal 
containers. Each metal container shall be independently capable of 
protecting the seeds and preventing spillage or escape. Each set of 
metal containers shall then be enclosed in a sturdy outer shipping 
container constructed of corrugated fiberboard, corrugated cardboard, 
wood, or other material of equivalent strength.
    (3) Live microorganisms and/or etiologic agents, cells, or 
subcellular elements. All regulated articles which are live (non-
inactivated) microorganisms, or etiologic agents, cells, or subcellular 
elements shall be packed as specified below:
    (i) Volume not exceeding 50 ml. Regulated articles not exceeding 50 
ml shall be placed in a securely closed, watertight container (primary 
container, test tube, vial, etc.) which shall be enclosed in a second, 
durable watertight container (secondary container). Several primary 
containers may be enclosed in a single secondary container, if the 
total volume of all the primary containers so enclosed does not exceed 
50 ml. The space at the top, bottom, and sides between the primary and 
secondary containers shall contain sufficient nonparticulate absorbent 
material (e.g., paper towel) to absorb the entire contents of the 
primary container(s) in case of breakage or leakage. Each set of 
primary and secondary containers shall then be enclosed in an outer 
shipping container constructed of corrugated fiberboard, corrugated 
cardboard, wood, or other material of equivalent strength.
    (ii) Volume greater than 50 ml. Regulated articles which exceed a 
volume of 50 ml. shall comply with requirements specified in paragraph 
(b)(3)(i) of this section. In addition, a shock absorbing material, in 
volume at least equal to that of the absorbent material between the 
primary and secondary containers, shall be placed at the top, bottom, 
and sides between the secondary container and the outer shipping 
container. Single primary containers shall not contain more than 1,000 
ml. of material. However, two or more primary containers whose combined 
volumes do not exceed 1,000 ml. may be placed in a single, secondary 
container. The maximum amount of micro-organisms or etiologic agents, 
cells, or subcellular elements which may be enclosed within a single 
outer shipping container shall not exceed 4,000 ml.
    (iii) Dry ice. If dry ice is used as a refrigerant, it shall be 
placed outside the secondary container(s). If dry ice is used between 
the secondary container and the outer shipping container, the shock 
absorbing material shall be placed so that the secondary container does 
not become loose inside the outer shipping container as the dry ice 
sublimates.
    (4) Insects, mites, and related organisms. Insects, mites, and 
other small arthropods shall be packed for shipment as specified in 
this paragraph or in paragraph (b)(3) of this section. Insects (any 
life stage) shall be placed in an escape-proof primary shipping 
container (insulated vacuum container, glass, metal, plastic, etc.) and 
sealed to prevent escape. Such primary container shall be placed 
securely within a secondary shipping container of crushproof styrofoam 
or other material of equivalent strength; one or more rigid ice packs 
may also be placed within the secondary shipping container; and 
sufficient packing material shall be added around the primary container 
to prevent movement of the primary shipping container. The secondary 
(styrofoam or other) container shall be placed securely within an outer 
shipping container constructed of corrugated fiberboard, corrugated 
cardboard, wood, or other material of equivalent strength.

[[Page 25134]]

    (5) Other macroscopic organisms. Other macroscopic organisms not 
covered in paragraphs (b)(1), (2), and (4) of this section which do not 
require continuous access to atmospheric oxygen shall be packaged as 
specified in paragraph (b)(3) or (b)(4) of this section. All 
macroscopic organisms which are not plants and which require continuous 
access to atmospheric oxygen shall be placed in primary shipping 
containers constructed of a sturdy, crush-proof frame of wood, metal, 
or equivalent strength material, surrounded by escape-proof mesh or 
netting of a strength and mesh size sufficient to prevent the escape of 
the smallest organism in the shipment, with edges and seams of the mesh 
or netting sealed to prevent escape of organisms. Each primary shipping 
container shall be securely placed within a larger secondary shipping 
container constructed of wood, metal, or equivalent strength material. 
The primary and secondary shipping containers shall then be placed 
securely within an outer shipping container constructed of corrugated 
fiberboard, corrugated cardboard, wood, or other material of equivalent 
strength, which outer container may have air holes or spaces in the 
sides and/or ends of the container, provided that the outer shipping 
container must retain sufficient strength to prevent crushing of the 
primary and secondary shipping containers.
    (c) Request for a variance from container requirements. A 
responsible person who believes the container requirements normally 
applicable to the movement of the person's regulated article(s) are 
inappropriate due to unique circumstances (such as the nature, volume, 
or life stage of the regulated article) may submit in an application 
for a permit, a request for a variance from the container requirements. 
The request for a variance under this section shall consist of a short 
statement describing why the normally applicable container requirements 
are inappropriate for the regulated article which the person proposes 
to move and what container requirements the person would use in lieu of 
the normally prescribed container requirements. USDA shall advise the 
responsible person in writing at the time a permit is granted on the 
person's request for a variance.


Sec.  340.9  Costs and charges.

    The services of the inspector during regularly assigned hours of 
duty and at the usual places of duty shall be furnished without 
cost.\13\ The U.S. Department of Agriculture will not be responsible 
for any costs or charges incident to inspections or compliance with the 
provisions of this part, other than for the services of the inspector.
---------------------------------------------------------------------------

    \13\ The Department's provisions relating to overtime charges 
for an inspector's services are set forth in 7 CFR part 354.
---------------------------------------------------------------------------

PART 372--NATIONAL ENVIRONMENTAL POLICY ACT IMPLEMENTING PROCEDURES

0
4. The authority citation for part 372 continues to read as follows:

    Authority: 42 U.S.C. 4321 et seq.; 40 CFR 1500-1508; 7 CFR 1b, 
2.22, 2.80, and 371.9.


0
5. Amend Sec.  372.5 as follows:
0
a. By adding paragraph (b)(7);
0
b. In paragraph (c)(3)(iii), by removing ``for'' and adding ``, or 
acknowledgment of notifications for,'' in its place; and
0
c. By adding paragraph (c)(4).
    The additions read as follows:


Sec.  372.5  Classification of actions.

* * * * *
    (b) * * *
    (7) Determination of nonregulated status for genetically engineered 
organisms.
    (c) * * *
    (4) Extending deregulations for genetically engineered organisms. 
Extension of nonregulated status under part 340 of this chapter to 
organisms similar to those already deregulated.
* * * * *

    Done in Washington, DC, this 9th day of June 2025.
Michael Watson,
Administrator, Animal and Plant Health Inspection Service.
[FR Doc. 2025-10797 Filed 6-13-25; 8:45 am]
BILLING CODE 3410-34-P