[Federal Register Volume 90, Number 104 (Monday, June 2, 2025)]
[Rules and Regulations]
[Pages 23281-23283]
From the Federal Register Online via the Government Publishing Office [www.gpo.gov]
[FR Doc No: 2025-09883]


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DEPARTMENT OF HEALTH AND HUMAN SERVICES

Food and Drug Administration

21 CFR Part 866

[Docket No. FDA-2025-N-1263]


Medical Devices; Immunology and Microbiology Devices; 
Classification of the Device To Detect Bacterial Protease Activity in 
Chronic Wound Fluid

AGENCY: Food and Drug Administration, HHS.

ACTION: Final amendment; final order.

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SUMMARY: The Food and Drug Administration (FDA, the Agency, or we) is 
classifying the device to detect bacterial protease activity in chronic 
wound fluid into class II (special controls). The special controls that 
apply to the device type are identified in this order and will be part 
of the codified language for the device to detect bacterial protease 
activity in chronic wound fluid's classification. We are taking this 
action because we have determined that classifying the device into 
class II (special controls) will provide a reasonable assurance of 
safety and effectiveness of the device. We believe this action will 
also enhance patients' access to beneficial innovative devices in part 
by reducing regulatory burdens.

DATES: This order is effective June 2, 2025. The classification was 
applicable on December 2, 2019.

FOR FURTHER INFORMATION CONTACT: Dina Jerebitski, Center for Devices 
and Radiological Health, Food and Drug Administration, 10903 New 
Hampshire Ave., Bldg. 66, Rm. 3574, Silver Spring, MD 20993-0002, 301-
796-2411, [email protected].

SUPPLEMENTARY INFORMATION: 

I. Background

    Upon request, FDA has classified the device to detect bacterial 
protease activity in chronic wound fluid as class II (special 
controls), which we have determined will provide a reasonable assurance 
of safety and effectiveness. In addition, we believe this action will 
enhance patients' access to beneficial innovation, in part by reducing 
regulatory burdens by placing the device into a lower device class than 
the automatic class III assignment.
    The automatic assignment of class III occurs by operation of law 
and without any action by FDA, regardless of the level of risk posed by 
the new device. Any device that was not in commercial distribution 
before May 28, 1976, is automatically classified as, and remains 
within, class III and requires premarket approval unless and until FDA 
takes an action to classify or reclassify the device (see 21 U.S.C. 
360c(f)(1)). We refer to these devices as ``postamendments devices'' 
because they were not in commercial distribution prior to the date of 
enactment of the Medical Device Amendments of 1976, which amended the 
Federal Food, Drug, and Cosmetic Act (FD&C Act).
    FDA may take a variety of actions in appropriate circumstances to 
classify or reclassify a device into class I or II. We may issue an 
order finding a new device to be substantially equivalent under section 
513(i) of the FD&C Act (see 21 U.S.C. 360c(i)) to a predicate device 
that does not require premarket approval. We determine whether a new 
device is substantially equivalent to a predicate device by means of 
the procedures for premarket notification under section 510(k) of the 
FD&C Act (21 U.S.C. 360(k)) and part 807 (21 CFR part 807).
    FDA may also classify a device through ``De Novo'' classification, 
a common name for the process authorized under section 513(f)(2) of the 
FD&C Act (see also part 860, subpart D (21 CFR part 860, subpart D)). 
Section 207 of the Food and Drug Administration Modernization Act of 
1997 (Pub. L. 105-115) established the first procedure for De Novo 
classification. Section 607 of the Food and Drug Administration Safety 
and Innovation Act (Pub. L. 112-144) modified the De Novo application 
process by adding a second procedure. A device sponsor may utilize 
either procedure for De Novo classification.
    Under the first procedure, the person submits a 510(k) for a device 
that has not previously been classified. After receiving an order from 
FDA classifying the device into class III under section 513(f)(1) of 
the FD&C Act, the person then requests a classification under section 
513(f)(2).
    Under the second procedure, rather than first submitting a 510(k) 
and then a request for classification, if the person determines that 
there is no legally marketed device upon which to base a determination 
of substantial equivalence, that person requests a classification under 
section 513(f)(2) of the FD&C Act.
    Under either procedure for De Novo classification, FDA is required 
to classify the device by written order within 120 days. The 
classification will be according to the criteria under section 
513(a)(1) of the FD&C Act. Although the device was automatically placed 
within class III, the De Novo classification is considered to be the 
initial classification of the device.
    We believe this De Novo classification will enhance patients' 
access to beneficial innovation, in part by reducing regulatory 
burdens. When FDA classifies a device into class I or II via the De 
Novo process, the device can serve as a predicate for future devices of 
that type, including for 510(k)s (see section 513(f)(2)(B)(i) of the 
FD&C Act). As a result, other device sponsors do not have to submit a 
De Novo request or premarket approval application to market a 
substantially equivalent device (see section 513(i) of the FD&C Act, 
defining ``substantial equivalence''). Instead, sponsors can use the 
less burdensome 510(k) process, when necessary, to market their device.

[[Page 23282]]

II. De Novo Classification

    On March 23, 2018, FDA received Alere Scarborough, Inc.'s request 
for De Novo classification of the WOUNDCHEK Bacterial Status device. 
FDA reviewed the request in order to classify the device under the 
criteria for classification set forth in section 513(a)(1) of the FD&C 
Act.
    We classify devices into class II if general controls by themselves 
are insufficient to provide reasonable assurance of safety and 
effectiveness, but there is sufficient information to establish special 
controls that, in combination with the general controls, provide 
reasonable assurance of the safety and effectiveness of the device for 
its intended use (see 21 U.S.C. 360c(a)(1)(B)). After review of the 
information submitted in the request, we determined that the device can 
be classified into class II with the establishment of special controls. 
FDA has determined that these special controls, in addition to the 
general controls, will provide reasonable assurance of the safety and 
effectiveness of the device.
    Therefore, on December 2, 2019, FDA issued an order to the 
requester classifying the device into class II. In this final order, 
FDA is codifying the classification of the device by adding 21 CFR 
866.3231.\1\ We have named the generic type of device as a device to 
detect bacterial protease activity in chronic wound fluid, and it is 
identified as a lateral flow prescription in vitro diagnostic device 
that may include a sterile swab. The device is intended for use in 
patients as an aid in assessing the risk for non-healing of chronic 
venous, diabetic foot, and pressure ulcers associated with wounds where 
there are no signs of wound infection and where patients are 
asymptomatic for clinical signs of infection.
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    \1\ FDA notes that the ACTION caption for this final order is 
styled as ``Final amendment; final order,'' rather than ``Final 
order.'' Beginning in December 2019, this editorial change was made 
to indicate that the document ``amends'' the Code of Federal 
Regulations. The change was made in accordance with the Office of 
Federal Register's (OFR) interpretations of the Federal Register Act 
(44 U.S.C. chapter 15), its implementing regulations (1 CFR 5.9 and 
parts 21 and 22), and the Document Drafting Handbook.
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    FDA has identified the following risks to health associated 
specifically with this type of device and the measures required to 
mitigate these risks in table 1.

 Table 1--Device To Detect Bacterial Protease Activity in Chronic Wound
                   Fluid Risks and Mitigation Measures
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  Identified risks to health              Mitigation measures
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Risk of false test results...  Use of certain specimen collection and
                                transport devices identified in special
                                control (1), Certain labeling
                                information identified in special
                                control (2), and Certain design
                                verification and validation activities
                                identified in special control (3).
Failure to correctly           Certain labeling information identified
 interpret test results.        in special control (2).
Failure to correctly operate   Certain labeling information identified
 the device.                    in special control (2).
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    FDA has determined that special controls, in combination with the 
general controls, address these risks to health and provide reasonable 
assurance of safety and effectiveness. For a device to fall within this 
classification, and thus avoid automatic classification in class III, 
it would have to comply with the special controls named in this final 
order. The necessary special controls appear in the regulation codified 
by this final order. This device is subject to premarket notification 
requirements under section 510(k) of the FD&C Act.

III. Analysis of Environmental Impact

    The Agency has determined under 21 CFR 25.34(b) that this action is 
of a type that does not individually or cumulatively have a significant 
effect on the human environment. Therefore, neither an environmental 
assessment nor an environmental impact statement is required.

IV. Paperwork Reduction Act of 1995

    This final order establishes special controls that refer to 
previously approved collections of information found in other FDA 
regulations and guidance. These collections of information are subject 
to review by the Office of Management and Budget (OMB) under the 
Paperwork Reduction Act of 1995 (44 U.S.C. 3501-3521). The collections 
of information in part 860, subpart D, regarding De Novo classification 
have been approved under OMB control number 0910-0844; the collections 
of information in 21 CFR part 814, subparts A through E, regarding 
premarket approval, have been approved under OMB control number 0910-
0231; the collections of information in part 807, subpart E, regarding 
premarket notification submissions, have been approved under OMB 
control number 0910-0120; the collections of information in 21 CFR part 
820, regarding quality system regulation, have been approved under OMB 
control number 0910-0073; and the collections of information in 21 CFR 
parts 801 and 809, regarding labeling, have been approved under OMB 
control number 0910-0485.

List of Subjects in 21 CFR Part 866

    Biologics, Laboratories, Medical devices.

    Therefore, under the Federal Food, Drug, and Cosmetic Act and under 
authority delegated to the Commissioner of Food and Drugs, 21 CFR part 
866 is amended as follows:

PART 866--IMMUNOLOGY AND MICROBIOLOGY DEVICES

0
1. The authority citation for part 866 continues to read as follows:

    Authority: 21 U.S.C. 351, 360, 360c, 360e, 360j, 360l, 371.


0
2. Add Sec.  866.3231 to subpart D to read as follows:


Sec.  886.3231  Device to detect bacterial protease activity in chronic 
wound fluid.

    (a) Identification. A device to detect bacterial protease activity 
in chronic wound fluid is a lateral flow prescription in vitro 
diagnostic device that may include a sterile swab. The device is 
intended for use in patients as an aid in assessing the risk for non-
healing of chronic venous, diabetic foot, and pressure ulcers 
associated with wounds where there are no signs of wound infection and 
where patients are asymptomatic for clinical signs of infection.
    (b) Classification. Class II (special controls). The special 
controls for this device are:
    (1) Any swab used to collect a patient specimen must be FDA-
cleared, -approved, or -classified as 510(k) exempt (standalone or as 
part of a test system) for the collection of wound fluid specimens; 
alternatively, the

[[Page 23283]]

sample collection device must be cleared in a premarket submission as a 
part of this device.
    (2) The labeling required under Sec.  809.10(b) of this chapter 
must include:
    (i) An intended use that includes the following statements:
    (A) A statement that the device detects and measures bacterial 
proteases from a swab saturated with wound fluid.
    (B) A statement that the device provides a qualitative output to 
aid the user in assessing the risk for non-healing of wounds (e.g., 
chronic venous, diabetic foot and pressure ulcers).
    (C) A description of the clinical indications for test use.
    (D) The specific population(s) for which the device is intended.
    (E) A description of the recommended training (e.g., knowledge and 
experience) for safe and effective use of the device and to minimize 
the risks of incorrect results and misinterpretation.
    (ii) A detailed description of the performance characteristics of 
the device from the analytical and clinical studies required under 
paragraphs (b)(3)(ii) and (iii) of this section.
    (iii) A detailed explanation of the interpretation of results.
    (iv) A warning statement describing situations where the device has 
not been validated or may not perform as identified in the labeling 
(e.g., not for use with wounds which are >=6 months of age and >=1 
cm\2\ in size).
    (v) The following limiting statements:
    (A) That the device is not intended to provide a risk assessment of 
chronic wound infection status or aid in the diagnosis of infection in 
chronic wounds, nor is the device intended for monitoring the 
effectiveness of anti-infective therapy.
    (B) That a negative result does not exclude the presence of 
bacterial proteases. Therefore, the results should be used in 
conjunction with clinical findings to make an accurate assessment of 
risk of nonhealing. The test result should be interpreted in 
conjunction with other risk factors, along with clinical and laboratory 
data available to the clinician.
    (C) That the device has been validated using wound fluid samples 
only. Other sample types (e.g., whole blood from venous or capillary 
draws, other body fluids) have not been evaluated.
    (D) That skin flora may secrete bacterial proteases therefore, swab 
contact with intact skin should be avoided as this may yield false 
positive results.
    (vi) Labeling must include a brief reference sheet for healthcare 
professionals that includes the intended use, summary of clinical 
performance, results from analytical testing on normal skin and human 
proteases, and warning and limiting statements.
    (3) Design verification and validation must include the following:
    (i) A detailed device description (e.g., all device parts, control 
elements incorporated into the test procedure, reagents required but 
not provided, and the principle of device operation and test 
methodology).
    (ii) Detailed documentation and results from analytical studies, 
including the limit of detection, inclusivity, cross-reactivity, 
microbial interference, analytical sensitivity for normal skin flora 
and human proteases, interfering substances, specimen stability, 
within-lab precision, and reproducibility.
    (iii) Detailed documentation and results from a clinical study that 
includes prospective (sequentially collected) samples for the intended 
specimen type that are representative of the intended use 
population(s). The clinical study must compare the device performance 
to results obtained from a reference or comparator method that FDA has 
determined is appropriate.

    Dated: May 27, 2025.
Grace R. Graham,
Deputy Commissioner for Policy, Legislation, and International Affairs.
[FR Doc. 2025-09883 Filed 5-30-25; 8:45 am]
BILLING CODE 4164-01-P