[Federal Register Volume 78, Number 228 (Tuesday, November 26, 2013)]
[Notices]
[Page 70537]
From the Federal Register Online via the Government Publishing Office [www.gpo.gov]
[FR Doc No: 2013-28354]


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DEPARTMENT OF COMMERCE

International Trade Administration


University of California, Berkely, et al.; Notice of Decision on 
Application for Duty-Free Entry of Scientific Instruments

    This is a decision pursuant to Section 6(c) of the Educational, 
Scientific, and Cultural Materials Importation Act of 1966 (Pub. L. 89-
651, as amended by Pub. L. 106-36; 80 Stat. 897; 15 CFR part 301). 
Related records can be viewed between 8:30 a.m. and 5:00 p.m. in Room 
3720, U.S. Department of Commerce, 14th and Constitution Ave. NW., 
Washington, DC.
    Docket Number: 13-002. Applicant: University of California, 
Berkeley, Berkeley, CA 94720. Instrument: High Speed Atomic Force 
Microscope (HSAFM). Manufacturer: Research Institute of Biomolecule 
Metrology (RIBM), Japan. Intended Use: See notice at 78 FR 7399-7400, 
February 1, 2013. Comments: None received. Decision: Approved. We know 
of no instruments of equivalent scientific value to the foreign 
instruments described below, for such purposes as this is intended to 
be used, that was being manufactured in the United States at the time 
of order. Reasons: The instrument will be used for a number of 
experiments including tracking the enzymatic activity of an RNA II 
polymerase along its template, a DNA gene, while synthesizing the 
messenger RNA. Having access to higher scan rates in an aqueous 
environment will provide an unprecedented view of transcription through 
nucleosomal DNA. By visualizing transcription steps, it is possible to 
precisely follow in real time the dynamics of events that accompany 
transcription by RNAP II through the nucleosome including spontaneous 
DNA unwrapping from the core particle, histone transfer, and histone 
dissociation under different conditions while determining the main 
factors that regulate nucleosome stability/instability during 
transcription. In addition to this capability, the instrument will have 
the time and spatial resolution to visualize individual tubulin 
subunits as they arrive at the microtubule end and will complement 
cryo-EM studies at near nanometer resolution on stabilized 
intermediates in the assembly process. The unique characteristics of 
this instrument are the ability to capture images at a rate of up to 
15-20 frames per second, reading scan rates as high as 25 frames per 
second, resonant frequencies of 3.5 MHz in air and 1.2 MHz in water, 
spring constants of 0.2 N m-1, a quality factor in water of 
~2, and a response time in water of ~0.5 microseconds.

    Dated: November 19, 2013.
Gregory W. Campbell,
Director, Subsidies Enforcement Office, Enforcement and Compliance.
[FR Doc. 2013-28354 Filed 11-25-13; 8:45 am]
BILLING CODE 3510-DS-P