[From the U.S. Government Printing Office, www.gpo.gov]
















                          A Transplant of Arctic Grayling to a Flooded
                         Gravel Mine Site in the Kuparuk River Oilfield

                                              by
                                        Jack F. Winters

                                   Technical Report No. 90-4






                                 U. C  r FFr T!FrI OF COMMERCE NOAA
                                 C,( i' "     "'    :T ,,  :" '? NT1ER
                                                 '  "'V - ,   VENUE
                                  *,-:.i..,t)t .  $   29405-2413




                                           Frank Rue
                                            Director
                                        Habitat Division
                              Alaska Department of Fish and Game
                                        P.O. Box 3-2000
                                     Juneau, Alaska 99802

                                          August 1990




   0 t_



                             r  lroperty of CSC Library



_N







                          Table of Contents



                                                          Page

List of Tables ....................................... iii

List of Figures .......................................iv

Acknowledgements .................................... v

Executive Summary ................................... vi

Introduction .........................................1

Arctic Grayling Transplant ................................2
     Introduction .................................... 2
     Methods ...................................... 3
     Results ....................................... 5
     Discussion .....................................5

Kuparuk River Arctic Grayling Disease Screening ................. 13
     Introduction ................................... 13
     Methods ......................................13
     Results ...................................... 13
     Discussion .................................... 13

References .........................................15

Appendix I: Length and estimated ages of arctic grayling
 captured within the Sagavanirktok River drainage and
 later transplanted to Kuparuk Mine Site B, June 1989 ...............16

Appendix II: Lengths of fish caught and released in
 Sagavanirktok River drainage streams, 21-25 June 1989 ............. 21

Appendix III: Lengths of fish caught in fyke nets in
 the Kuparuk River and Smith Creek downstream of the
 Spine Road crossing, 20-21 July 1989 ........................25








                                 List of Tables


                                                                      Page

Table 1.      Size category, number, and origin of
             arctic grayling transplanted to Kuparuk
             Mine Site B, June 1989, from the
             Sagavanirktok River drainage ......................6

Table 2.      Estimated growth rates of individual
             arctic grayling transplanted to Kuparuk
             Mine Site B, summer 1989 ....................... 7

Table 3.      Age-length relationships for arctic
             grayling captured within the Sagavanirktok
             River drainage and later transplanted to
             Kuparuk Mine Site B, June 1989 .................... 8








                                 List of Figures


                                                                      Page

Figure 1.     Capture locations and sampling techniques
             used to capture arctic grayling, June 1989 ...............4

Figure 2.     Kuparuk Mine Site B, depicting the
             locations of the inlet and interconnecting
             channels excavated in May 1989 ................... 10











































                                       -iv-








                               Acknowledgements

Matt Robus and Carl Hemming assisted with the capture and transplanting of the arctic
grayling. Roger Post, Phyllis Weber-Scannell, and Carl Hemming assisted with fish
sampling in the Kuparuk River.

Financial assistance for field work and this report was provided by the Kuparuk River
Unit owners and the Prudhoe Bay Unit owners through a grant to the ADF&G Habitat
Division. Steve Taylor (BP) and Mike Joyce (ARCO) helped in supporting efforts to
obtain the grant.  Financial assistance was also provided by the Alaska Coastal
Management Program through the Coastal Zone Management Act of 1972, as amended,
and administered by the Office of Ocean and Coastal Resource Management, National
Oceanic and Atmospheric Administration.






































                                        -v-V








                                Executive Summary

Arctic grayling were captured at seven locations within the Sagavanirktok River drainage
near Happy Valley Creek 21-25 June 1989. Two hundred ten arctic grayling, ranging in
length from 176 to 399 mm, were transplanted to Kuparuk Mine Site B on 26-27 June
1989. Additional sampling is planned for 1990 and subsequent years to assess the
success of this experimental fish transplant.

ARCO Alaska, Inc. completed a habitat enhancement project in May 1989 at Kuparuk
Mine Site B that contained features that ADF&G believed would increase the long-term
success of the arctic grayling transplant. A description of these features, their importance
to the long-term success of the transplant, and their benefits to other fish and wildlife at
the site is presented.

Sampling in the Kuparuk River downstream of the Spine Road crossing indicated that
limited numbers of large arctic grayling use this area in mid July. Numbers of large
arctic grayling were insufficient to conduct disease screening of arctic grayling in the
Kuparuk River at this time.



























                                         -vi-









                                 INTRODUCTION


Since 1986, the Alaska Department of Fish and Game (ADF&G) has conducted
limnological and fish sampling in selected flooded gravel mine sites in the Prudhoe Bay -
Kuparuk oilfields to determine if these sites would provide suitable habitat for fish and
wildlife. Sampling in 1986 and 1987 concentrated on the chemical and physical features
of the sites (Hemming 1988). Sampling in 1988 focused on limnological sampling
directed at identifying features of each site that could influence algal productivity and
zooplankton densities.   This sampling provided additional chemical and physical
information, indications of productivity of the sites, and estimates of densities and
species of zooplankton that could be used by fish for food (Hemming et al. 1989). With
the results of these studies, ADF&G determined that Kuparuk Mine Site B, a flooded
gravel mine site with limited potential for colonization by most freshwater fish, contained
sufficient habitat to support a population of arctic grayling. As a result of these studies,
ADF&G transplanted arctic grayling to this site in 1989.

There are two components to this technical report. The first component describes the
capture and transplanting of Sagavanirktok River drainage arctic grayling to Kuparuk
Mine Site B  in June 1989.  This section also describes site rehabilitation efforts
conducted by the oil industry at Kuparuk Mine Site B and the potential benefits of these
efforts to fish and wildlife at this site. The second component describes fish sampling in
the Kuparuk River to obtain arctic grayling for disease screening so that this river system
could be used as a source of arctic grayling for future transplants to mine sites within the
oilfields.



















                                          -1-







                      ARCTIC GRAYLING TRANSPLANT


Introduction

Within the Prudhoe Bay-Kuparuk oilfields are several tundra streams that discharge
directly to the Beaufort Sea, have limited populations of fish, and have limited potential
for colonization by freshwater fish from distant streams. Brackish or marine conditions
that exist in the nearshore Beaufort Sea usually provide a barrier to movements of
freshwater fish, such as arctic grayling, beyond the mouths of streams and thereby limit
colonization of distant streams by these salt-intolerant fish. These tundra streams also
contain limited habitat suitable to fish for overwintering, as the streams are shallow and
generally freeze to the bottom in winter. Two of these streams now connect with deep
flooded gravel mine sites that provide conditions required to overwinter fish. Such
conditions in these two stream systems provide an opportunity to determine if a common
arctic freshwater fish, arctic grayling, that does not occur in these stream systems, can be
introduced, survive, and reproduce in a mine site/stream system. With this opportunity,
the Alaska Department of Fish and Game in June 1989 conducted an experimental
introduction of arctic grayling into one tundra stream/mine site system, East
Creek/Kuparuk Mine Site B.

The objective of the transplant was to establish a reproducing population of arctic
grayling in East Creek/Kuparuk Mine Site B. Our goal was to obtain 200-500 large
arctic grayling from the Sagavanirktok River drainage that could be transplanted to the
mine site. Arctic grayling from the Sagavanirktok River drainage were screened for
diseases, and approved for transplanting in Kuparuk Mine Site B in 1988 (Hemming et
al. 1989). Hemming et al. (1989) collected arctic grayling for disease screening in the
lower Sagavanirktok River drainage during mid-to-late summer and found too few for
transplanting.   The  Happy  Valley Creek  area, about  130 km  upstream in the
Sagavanirktok River drainage, contains several streams with abundant large arctic
grayling in early summer. Thus, we collected all fish from this area for transplanting to
Kuparuk Mine Site B.

The following component describes the results of the arctic grayling capture and
transplant and some initial observations on the success of the transplant. This component
also describes the modifications made to the Kuparuk Mine Site B/East Creek system to
enhance its ability to support fish and that may directly contribute to the success of the
experimental transplant.



                                         -2-








Methods

We attempted to capture arctic grayling at 10 locations near Happy Valley Creek 21-25
June 1989 (Figure 1). We captured arctic grayling at seven locations with several types
of sampling equipment: fyke nets, seines, angling, and dip net. Limited sampling with a
backpack electroshocker did not yield fish. Fyke nets were most effective at the mouths
of tundra streams where stream velocities were low, and stream depth and width allowed
adequate placement of the nets. One fyke net was also set in one pond of Goose Green
Gulch, a former gravel mine site. We checked fyke nets daily for fish, and measured and
released captured fish other than arctic grayling, and arctic grayling less than 176 mm.

We placed captured arctic grayling suitable for transplant in an insulated cooler and
transported them from the capture site to a holding pen in lower Happy Valley Creek.
When necessary, supplemental oxygen delivered through aquarium airstones kept the
water in the cooler well oxygenated. Before placing the arctic grayling in the holding
pen, we anesthesized the fish with MS 222 (tricane methane sulfonate), measured them to
the nearest millimeter (fork length), and removed scales for age estimation. We also
tagged the fish at the base of their dorsal fin with numbered yellow floy tags, that will
allow monitoring of the growth of the fish. A 1.2 x 2.4 x 1.2 m covered net pen held the
arctic grayling for up to 5 days before transport to Kuparuk Mine Site B.

We transported approximately 100 arctic grayling to Kuparuk Mine Site B on both 26
and 27 June 1989. Two 114 L plastic garbage containers lined with a large plastic bag
containing about 95 L of water and a similarly lined 64 L insulated cooler containing
about 50 L of water held the fish during transport. Plastic bags were tied to prevent loss
of water and fish. Supplemental oxygen delivered through aquarium airstones during
loading of the fish into the transport containers and the subsequent 3 hr drive to Kuparuk
Mine Site B reduced the possibility of suffocation. We released the arctic grayling at the
northeast section of Kuparuk Mine Site B upon arrival at the site.

We placed two fyke nets within Kuparuk Mine Site B on 23 and 24 August in part to
 determine if arctic grayling were still present in the site. On 5 October we again sampled
 for arctic grayling, on this occasion by angling.









                                           -3-






                           / /t Mark Creek




                          /








                     0 |Happy Valley Creek
                        !









                     -  \   & ! Dan Creek
                        Lori Creek 
                  O4 ,









                 ' l Q Grader Slough
                      _   Goose Green Gulch
                                                      ï¿½ Fyke Net
                                                       A Electroshocking
                                                      ï¿½ Seine
                                                      0 Angling
           S    ~Gustafson Gulch                     4 Dip Net

                     f  HPo   lygon  Creek
                 /











                    Poison Pipe Creek



                    0  Oksrukuyik Creek                    kilometers
                   UrooearenGuc










June 1989.


   -4-Figure 1. Capture locations and sampling techniques used to capture arctic grayling,



                                     -4-








Results

We placed 210 arctic grayling, ranging in length from 176 to 399 mm, in Kuparuk Mine
Site B on 26 and 27 June 1989 (Appendix 1). The average size of the transplanted arctic
grayling was 283 + 52 mm (Table 1). All arctic grayling appeared healthy when released
and several began feeding at the surface within minutes of their release. One arctic
grayling swam out of Kuparuk Mine Site B and into East Creek immediately upstream of
the Spine Road culverts within 15 min of its release.

Oil industry personnel caught and kept at least two arctic grayling (an unknown number
were also caught and released) within the first few weeks after stocking (S. Bishop,
Wildlife Biologist, ADF&G, Fairbanks, pers. comm.). Although the site was not closed
to sport fishing following the transplant, ADF&G staff requested anglers to release all
tagged arctic grayling that they caught. In August, ARCO Alaska, Inc. placed a sign
describing the transplant project and requesting release of tagged arctic grayling at
Kuparuk Mine Site B.

We caught two arctic grayling in fyke nets set in Kuparuk Mine Site B on 23 and 24
August. One arctic grayling did not have a numbered floy tag but had a wound at the
base of its dorsal fin, suggesting this fish had lost its tag and was a transplanted fish. The
other arctic grayling grew at an average rate of 0.23 mm/day over the 61 days between its
initial and subsequent capture (Table 2).

ADF&G staff captured and released three arctic grayling in Kuparuk Mine Site B by
 angling on 5 October, including one previously captured in a fyke net on 23 August.
 Average rates of growth for these arctic grayling over a 102-105 day period ranged from
 0.24 to 0.34 mm/day (Table 2).

 Based on scale annuli, we estimated the ages of transplanted arctic grayling to range from
 3 to 11 yrs (Table 3). The age-length relationship is highly correlated:  age = 0.0383
 (length [mm]) - 4.62; r2 = 0.84. Estimated ages of the transplanted arctic grayling were
 similar to those estimated for arctic grayling obtained from the lower Sagavanirktok
 River in 1988 (Hemming et al. 1989).

 Discussion

 Before the introduction of arctic grayling into Kuparuk Mine Site B, ARCO Alaska, Inc.
 completed a habitat enhancement project that contained features ADF&G believed would




                                           -5-









Table 1. Size category, number, and origin of arctic grayling transplanted to Kuparuk Mine Site B,
        June 1989, from the Sagavanirktok River drainage.




                                              Number of Arctic Gravlin~                Mean Length
Capture Site (Capture Method)            Total          <300 mm        >300 mm         (mm) + s.d.


Happy Valley Creek (fyke net)           52                 26              26            281 + 55

Dan Creek (dip net)                       1                 0                1           330

Mark Creek (2 fyke nets)                39                 15              24            294 + 60

Oksrukuyik Creek (fyke net)              12                 6               6            283 + 51

Grader Slough (angling)                  7                  5               2            258 + 49

Gustafson Gulch (seine)                  13                12                1           243 + 45

Goose Green Gulch (fyke net)            86                 44              42            288 +ï¿½ 45

 All Sites                              210                                               283 +ï¿½ 52








Table 2. Estimated growth rates of individual arctic grayling transplanted to Kuparuk
      Mine Site B, summer 1989.



                                 Estimated                  Estimated
            Length (mm)          Growth       Days in       Growth Rate
At Capture       At Recapture    (mm)          Mine Site    (mm/day)


310*               324            14             61           0.23
310*               335            25            104           0.24
299                324            25            105           0.24
335                370            35            102           0.34


* same fish





































                                       -7-








Table 3. Age-length relationships for arctic grayling captured within the Sagavanirktok
       River drainage and later transplanted to Kuparuk Mine Site B, June 1989. Ages
       estimated from scale annuli; n = number of fish in sample; sd = standard
       deviation.



                                              Fork Length (mm)
Age                          n             Mean           Range         sd


3                          28             200.8         176-246        16.2
4                          29             228.8          180-292       25.2
5                          21             262.7         225-298        18.3
 6                          28             291.0         254-324        18.9
7                          23             315.9         281-334        14.1
 8                          30             321.3         287-353        14.5
 9                          18             334.8         299-376        20.4
10                           4            344.2          316-368       21.7
11                           6            365.8          356-399       16.4

TOTAL                      187
































                                           -8-








increase the long-term success of the arctic grayling transplant. The ADF&G had two
objectives for the enhancement project: to connect the two separate basins with two
channels to form one contiguous lake with an island; and to connect the mine site and
East Creek with a permanent channel. ARCO Alaska, Inc. completed this enhancement
project in May 1989. In addition, ARCO Alaska, Inc. previously removed fill and
culverts from East Creek upstream of Kuparuk Mine Site B in late summer 1988.

ARCO Alaska, Inc. excavated three channels during the enhancement project (Figure 2).
Two channels, approximately 15 m wide, 15 m long, and I m deep, cut through the
ground separating the two basins of the mine site, create an island approximately 30 m x
15 m. A third channel, excavated between East Creek and the southwest corner of the
southernmost basin is approximately 20 m wide, 25 m long, and 2 m deep. The
excavated material was stockpiled next to existing overburden on the east side of the site,
and on an existing overburden stockpile on the south side of the site.

The connections between the two basins and between the site and East Creek were deep
enough to contain adequate amounts of water throughout the summer. Previously, the
connection between East Creek and Kuparuk Mine Site B was marginal during periods of
low flow. The new connection provided an adequate channel between the site and the
creek, yet flow continued through the original stream channel that bordered the western
edge of the site. There was some concern during the planning of this enhancement
project that by constructing a deep connection between East Creek and Kuparuk Mine
Site B, the creek would flow through the mine site, and exit at the site's northwest corner,
thereby eliminating flow through a portion of the original stream channel. During our
 site visits, some of which were during periods of low stream flow, water continued to
 flow through the original channel.

 The permanent connection of Kuparuk Mine Site B with East Creek provides several
 benefits to the site's fish and wildlife. The connection may now divert a significant
 portion of the spring runoff of East Creek through the site, promoting more rapid ice melt
 within Kuparuk Mine Site B. Rapid ice melt should provide areas of open water that
 may be used by surface feeding fish and by waterfowl. The tundra stream water also
 should be warmer at times during spring and summer than mine site water, and may serve
 to warm the lake. Water from East Creek also should bring additional nutrients to mine
 site waters, enhancing the productivity of the system. The permanent connection will
 enable arctic grayling to move freely out of the mine site to areas in the creek that may be
 suitable for feeding or spawning, and then into the mine site when the creek freezes.



                                          -9-












































                                                      A  fyke net locations




                                                      meters




Figure 2. Kuparuk Mine Site B, depicting the locations of the inlet and interconnecting
       channels excavated in May 1989. Map drawn from July 1989 aerial photograph.





                                        -10-








We selected several sites for capture of arctic grayling in the Happy Valley Creek area
for three reasons: to collect the desired number of fish rapidly; to obtain fish from a
variety of locations after they had spawned; and to minimize the potential of removing a
significant portion of the adult population of a particular stream. Netting at the mouths of
streams in late June increased the chances that some arctic grayling captured at a
particular stream had spawned, or that the arctic grayling were from other systems and
were using the stream mouths as feeding areas or as intermediate stops between other
areas. McCart et al. (1972) reported that arctic grayling from Happy Valley Creek
disperse to the Sagavanirktok River and other mountain streams after spawning. Craig
and Poulin (1975) also noted similar patterns of movement by arctic grayling in Weir
Creek in the Kavik River system (approximately 80 km northeast of Happy Valley).
These studies also suggested that by mid-June, most arctic grayling have spawned in their
tundra streams and have begun downstream movements. During our capture of arctic
grayling, we noted no evidence of sex products from the fish we handled. Thus, the
arctic grayling we removed from these systems probably spawned before their capture.

Forty-one percent (86) of the 210 transplanted arctic grayling were from Goose Green
Gulch, a site that contains about 1.5 ha of ponds connected to the Sagavanirktok River.
These fish were likely transients from another stream system as conditions in this site did
not appear favorable for spawning by arctic grayling. Arctic grayling use several tundra
streams that drain into the Sagavanirktok River and that are within several kilometers of
Goose Green Gulch. Although possible, it is unlikely that all arctic grayling caught at
Goose Green Gulch were from any particular one of these streams. It is more likely that
these and other streams contributed individuals to this catch and that the removal of these
86 individuals did not affect any particular arctic grayling population severely.

We do not know the extent to which the transplanted arctic grayling used East Creek. At
least one arctic grayling moved into the creek shortly after its release into Kuparuk Mine
 Site B. Water levels and wind produced conditions that made arctic grayling difficult to
 see in East Creek during our visits throughout the summer. Sampling with fyke nets in
late August in Kuparuk Mine Site B yielded only two arctic grayling, suggesting that
 some arctic grayling may have been in the creek at this time. Conversely, arctic grayling
 may not have been captured at these net sites because only two nets fished for two days,
 or because arctic grayling may not have intercepted the nets.

 The presence of arctic grayling in Kuparuk Mine Site B in early October suggests that
 some arctic grayling would overwinter within the mine site. We do not know whether



                                          -11-








these fish spent the entire summer in Kuparuk Mine Site B or ventured into East Creek
and returned after some undetermined period.  Sampling in late winter or spring,
preferably before the arctic grayling leave the site for the creek, will be required to assess
overwinter survival of these fish.

Large numbers of ninespine stickleback in the Kuparuk Mine Site B/East Creek system
may affect the success of the transplant of arctic grayling, through competition with fry
and juvenile arctic grayling, should successful spawning by arctic grayling occur in this
system. Our sampling in late August in Kuparuk Mine Site B produced overnight catches
of an estimated 23,000-24,000 ninespine stickleback. Skaugstad (1989) noted poor
growth of stocked arctic grayling fingerlings and little or no apparent survival of sac fry
in interior Alaska ponds containing threespine stickleback. Whether competition occurs
between ninespine stickleback and arctic grayling will depend upon the relative densities
of each species, the microhabitats used by each species and their degree of overlap, the
degree to which prey species overlap, and the availability of alternative prey. Potential
predation by adult ninespine stickleback on arctic grayling sac fry also may adversely
affect survival of arctic grayling fry.

A complete assessment of the success of the arctic grayling transplant experiment will
require several years of continued sampling within the Kuparuk Mine Site B/East Creek
system. Since arctic grayling within and near the Sagavanirktok River drainage mature
between ages 4 and 8 (Craig and Poulin 1975, McCart et al. 1972), eight years may be
required to determine if any progeny of the transplanted adults successfully spawns and
continues to perpetuate arctic grayling within the Kuparuk Mine Site B/East Creek
system. Sampling will be needed to detect the presence of fry and thus successful
spawning by the stocked adults. Further sampling will be required to determine if any
arctic grayling fry survive beyond initial hatching and contribute to the juvenile
component of the population. Sampling also should reveal the relative survival of any
progeny of the transplanted adults.














                                          -12-







       KUPARUK RIVER ARCTIC GRAYLING DISEASE SCREENING


Introduction

To comply with our Fish Transport Permit, we attempted to obtain 60 arctic grayling
from the Kuparuk River for disease screening. Approval to use Kuparuk River arctic
grayling would provide two benefits: an additional source of fish other than the
Sagavanirktok River system, and a source of fish minutes from potential stocking
locations as opposed to hours for some Sagavanirktok River sites.

Methods

During the period 19-21 July, we fished one fyke net in each of three locations in slack
water channels of the Kuparuk River: 1.6, 2.4, and 4.8 km downstream of the Spine
Road crossing. We fished one additional net at the mouth of Smith Creek, about 3.2 km
downstream of the Spine Road crossing. We checked the nets daily and kept all arctic
grayling greater than 170 mm in a 1.2 x 2.4 x 1.2 m net holding pen in Kuparuk Deadarm
Reservoir 5. Each day we released all arctic grayling less than 170 mm and all other fish
after measuring them to the nearest millimeter (fork length). After two days we ended
sampling and returned all of the arctic grayling held in the holding pen at Kuparuk
Deadarm Reservoir 5 to Smith Creek (all of the penned arctic grayling were from Smith
Creek).

Results

Fyke nets set in slack water channels of the lower Kuparuk River captured 52 arctic
grayling, 2 slimy sculpin, and 12 ninespine stickleback. These 66 fish were small, less
than 130 mm in length (Appendix 3). The fyke net set at the mouth of Smith Creek for 2
days captured 4 ninespine stickleback, a 465 mm broad whitefish, and 26 arctic grayling
from 79 to 434 mm long (Appendix 3).

Discussion

Catch rates of large arctic grayling in the lower Kuparuk River in late July precluded
obtaining an adequate sample of arctic grayling for disease screening. At least 7 to 10
days would have been required to capture the 60 arctic grayling needed for disease
screening. As Smith Creek was the only site that produced any arctic grayling suitable
for screening, it is likely that all arctic grayling used for disease screening would have to




                                        -13-








come from Smith Creek or other small tributaries to the Kuparuk River. The number of
streams tributary to the Kuparuk River in the vicinity of the Spine Road crossing is small
and removal of the number of arctic grayling necessary for disease screening or a
transplant could have adverse effects on the populations of arctic grayling in these
streams.

Our sampling indicates that there are insufficient large arctic grayling at catchable
locations in the Kuparuk River in mid July to successfully conduct a transplant operation
should this arctic grayling stock be approved for transplanting. Arctic grayling may be
more numerous in the lower Kuparuk River in late August if this segment of the river is
used by arctic grayling as an overwintering area. Additional sampling in late August
may indicate that sufficient numbers of large arctic grayling are available for disease
screening and transplanting immediately before freeze-up.





































                                          -14-








References

Craig, P.C., and V.A. Poulin. 1975. Movements and growth of arctic grayling
       (Thymallus arcticus) and juvenile arctic char (Salvelinus alpinus) in a small arctic
       stream, Alaska. J. Fish. Res. Board Can. 32(5):689-697.

Hemming, C.R. 1988. Aquatic habitat evaluation of flooded North Slope gravel mine
       sites (1986-1987). Alaska Department of Fish and Game, Habitat Division Tech.
       Rept. No. 88-1. Juneau. 69 pp.

Hemming, C.R., P.K. Weber, and J.F. Winters. 1989. Limnological and fisheries
       investigations of flooded North  Slope gravel mine  sites, 1988.   Alaska
       Department of Fish and Game, Habitat Division Tech. Rept. No. 89-1. Juneau.
       60 pp.

McCart, P., P. Craig, and H. Bain. 1972. Report on fisheries investigations in the
       Sagavanirktok River and neighboring drainages. Prepared for Alyeska Pipeline
       Service Co. 143 pp.

Skaugstad, C.  1989. Evaluation of arctic grayling enhancement:  a cost per survivor
       estimate. Alaska Department of Fish and Game Fishery Data Series No. 96. Div.
       of Sport Fish, Juneau. 68 pp.







Appendix 1.  Length and estimated ages of arctic grayling captured within the
      Sagavanirktok River drainage and later transplanted to Kuparuk Mine Site B,
      June 1989.

Creek (Date of capture)        Length        Age        Tag #
                               (mm)         (yr)

Dan Creek (6/22/89)             330           9         002039

Goose Green Gulch (6/25/89)     183           3         002167
                                189           3         002164
                                193           3         002165
                                194           4         002151
                                198           3         002160
                                203           3         002206
                                206           3         002149
                                212           3         002208
                                215           3         002132
                                216           3         002144
                                219           4         002139
                                227           4         002174
                                229           4         002183
                                230           4         002202
                                235           4         002136
                                246           3         002148
                                246           4         002200
                                251           4         002135
                                253           5         002166
                                259           4         002169
                                260           5         002182
                                262           4         002181
                                264           5         002157
                                265           5         002201
                                269           6         002172
                                271           5         002145
                                273           -         002187
                                276           5         002130
                                277           5         002177
                                277           3         002193
                                281           5         002186
                                282           6         002190
                                283           5         002134
                                284           6         002191
                                286           6         002155
                                289           6         002168
                                289           5         002161
                                290           6         002210
                                290           6         002211
                                292           4         002196
                                295           7         002129
                                295           -         002147
                                296           8         002173




                                    -16-




Appendix 1 continued.


Creek (Date of capture)        Length       Age         Tag #
                              (ram)        (yr)

Goose Green Gulch (6/25/89)    296            6         002204
                               298           5         002128
                               301           6         002158
                               301           6         002162
                               303           6         002171
                               306           7         002207
                               306           8         002209
                               307           -         002203
                               309           -         002137
                               311           -         002143
                               312           6         002152
                               312           7         002175
                               312           7         002176
                               314           7         002199
                               315           8         002146
                               315           7         002192
                               317           6         002126
                               320           -         002178
                               321           8         002154
                               322           7         002197
                               323           7         002159
                               323           -         002170
                               323           -         002188
                               324           6         002127
                               324           6         002184
                               324           7         002195
                               326           8         002131
                               328           8         002205
                               329           8         002138
                               329           -         002156
                               329           -         002179
                               330           7         002153
                               331           7         002163
                               332           7         002141
                               335           -         002194
                               335           8         002198
                               337           8         002150
                               341           8         002185
                               347           9         002125
                                350           -         002180
                               351          10         002133
                                352           -         002140
                               353           8         002142
                                360           -         002189
Grader Slough (6/24/89)        216            3         002122
                                227           4         002120
                                229           -         002118





                                   -17-




Appendix 1 continued.



Creek (Date of capture)        Length        Age        Tag #
                               (mm)         (yr)

Grader Slough (6/24/89)         285           6         002124
                               291           7         002119
                               313           8         002121
                               342          10         002123

Gustafson Gulch (6/21/89)       176           3         002002
                               225           4         002003
                               232           4         002001
                               274            -        002004
                               313            -        002006

(6/23/89)                       177           3         002088
                               201           3         002090
                               210           3         002096
                               241           5         002089
                               241           4         002098
                               254           6         002087
                               267           5         002091
                               297           6         002097

Happy Valley Creek (6/22/89)    193           4         002002
                                199           4         002029
                                218           4         002012
                                226           4         002017
                                227           4         002008
                                232           5         002022
                                237            -        002033
                                250           5         002016
                                276           6         002038
                                292           6         002027
                                297            -        002023
                                299           9         002032
                                304           6         002018
                                307           8         002014
                                307            -        002024
                                307           8         002037
                                308           8         002015
                                311           6         002009
                                311           8         002034
                                316          10         002019
                                320           9         002007
                                321           7         002010
                                321           7         002013
                                324           8         002028
                                326           -         002036
                                328           7         002021
                                329           7         002020
                                330           8         002039
                                336           9         002030
                                356          11         002031



                                    -18-




Appendix i continued.


Creek (Date of capture)        Length        Age        Tag #
                               (nun)        (yr)

Happy Valley Creek (6/22/89)   358           11         002035
                               359          11         002025
                               368          10         002011

(6/23/89)                       191           3         002081
                               201           3         002080
                               203           3         002075
                               206           3         002082
                               218           4         002079
                               238            -        002085
                               238           4         002088
                               265           6         002076
                               281           7         002077
                               287           8         002072
                               310           9         002073
                               314           9         002074
                               315           8         002078
                               315           8         002086
                               317           7         002083
                               399          11         002084

(6/24/89)                       185           3         002099
                                188           3         002102
                                194           3         002101
                               257           5         002100

Mark Creek (6/23/89)            180           3         002048
                                180           4         002051
                                187           4         002058
                                209           3         002056
                                214           4         002043
                                214           3         002054
                                217           4         002052
                                221           3         002065
                                239           4         002050
                                256           6         002044
                                266           5         002060
                                301            -        002045
                                312           9         002069
                                316           7         002046
                                316           8         002070
                                317           8         002063
                                318           9         002064
                                325            -        002068
                                329           8         002061
                                331           8         002062
                                332           8         002071
                                334           7         002066
                                337           8         002059
                                343           9         002049



                                    -19-




Appendix 1 continued.


Creek (Date of capture)          Length         Age         Tag #
                                 (mm)          (yr)

Mark Creek (6/23/89)              347            9          002055
                                 348            9          002057
                                 349            9          002067
                                 362           11          002053
                                 376            9          002047

(6/24/89)                         212            3          002109
                                 232            4          002104
                                 260            5          002103
                                 266            6          002110
                                  329            8          002107
                                  330            9          002108
                                  330            9          002112
                                  355            9          002111
                                  362             -         002105
                                  363            9          002106

Oksrukuyik Creek (6/22/89)        225            5          002042
                                  296            7          002040
                                  310            6          002041

(6/23/89)                         258            5          002093
                                  286            6          002092
                                  307            8          002094
                                  361           11          002095

(6/24/89)                         176            3          002113
                                  244            5          002116
                                  278            6          002114
                                  315            7          002117
                                  338            8          002115





















                                      -20-







Appendix 2. Lengths of fish caught and released in Sagavanirktok River drainage
      streams, 21-25 June 1989.


                                   Length of Fish in Millimeters

                               Happy                                  Goose
                   Mark         Valley      Gustafson    Oksrukuyik  Green
Species            Creek        Creek         Gulch         Creek      Gulch


Arctic Grayling      54           59           115           65           66
                    61           64           138           68           66
                    64           64           148           71           68
                    68           67           151           76           69
                    68           68             -          146           70
                    72           68             -          155           71
                    72           69                          -           72
                    73           70                          -           72
                    77           71                          -           73
                     78           73                          -           75
                     79           73                          -           75
                     86           75                          -           76*
                     90           77                          -           78
                     91           77             -            -           79
                     92           77                          -          104
                     95           78                          -          109
                    101          78              -            -          110
                    103          79              -            -          113
                    104           81*            -            -          115
                    106           85             -            -          115
                    110          106             -            -          128
                    110          108             -            -          139
                    112          110             -            -          144
                    113          111             -            -          157
                    115          112             -            -          158
                    116          113             -            -          162
                    119          113             -            -          163
                    120          113             -            -          164
                    122          115             -            -          165
                    124          116             -            -          169
                    128          117             -            -          176
                    147          117
                    150          118
                    151          121
                    155          124             -            -
                    155          124             -            -            -
                    155          124             -            -
                    155          125             -            -
                    157          125             -            -
                    160          125             -             -
                    163          126




                                     -21-




Appendix 2 continued.



                                   Length of Fish in Millimeters

                               Happy                                 Goose
                  Mark        Valley       Gustafson    Oksrukuyik  Green
Species            Creek        Creek         Gulch        Creek      Gulch


Arctic Grayling     164         127
                   167         132
                   171         139
                   182         141
                   183         146
                   191         152
                    192         153
                    193         153
                   209          154
                   218          155
                   302          155
                      -         156
                                156
                                157
                                159
                                159
                      -         162
                                163
                                166
                                166
                      -         167
                                170
                      -         172
                                300
                                324*
                                328
                                347

Round Whitefish     153         268              -328                   332
                    249         275              -          333         335
                    249         284              -          345
                    265         309              -          348
                    272         312
                    288         312
                    293         329
                    306         336
                    306         345
                    307         349
                    314
                    319
                    320
                    324
                    334
                    342
                    347



                                     -22-





Appendix 2 continued.




                                   Lenath of Fish in Millimeters

                               Happy                                 Goose
                   Mark        Valley       Gustafson    Oksrukuyik  Green
Species           Creek        Creek         Gulch        Creek      Gulch


Round Whitefish    368
                    378           -             -
                    378           -             -            -            -

Burbot             500            -             -         415             -
                    550           -             -          430            -
                                   -  -          -         455            -
                                   -  -          -         455            -
                                   -  -          -         462            -
                                   -  -          -         484            -
                                   -  -          -         486            -
                                   -  -          -         493            -
                                   -  -          -         495            -
                                   -  -          -         500            -
                                   -  -          -         501            -
                                   -  -          -         502            -
                                   -  -          -         505            -
                                   -  -          -         510            -
                                   -  -          -         510            -
                                   -  -          -         510            -
                                   -  -          -         520            -
                                   -  -          -         520            -
                                                 -  -      520            -
                                   -             -         520            -
                                   -  -          -         520            -
                                   -  -          -         525            -
                                   -  -          -         530            -
                                   -  -          -         540            -
                                   -  -          -         600            -
                                   -  -          -         600            -
                                   -  -          -         600            -

Dolly Varden          -          68             -           85            -
                                  70             -         118
                                  72             -            -
                                  75             -            -
                                  76             -            -
                                  77             -            -
                                  77             -            -
                                  96             -            -
                                 101             -            -
                                 102             -            -
                                 103             -            -
                                 105             -            -
                                 107             -            -




                                     -23-




Appendix 2 continued.



                                        Length of Fish in Millimeters

                                   Happy                                    Goose
                     Mark         Valley        Gustafson    Oksrukuyik   Green
Species             Creek         Creek           Gulch         Creek       Gulch


Dolly Varden            -          193                                           -

Ninespine Stickleback   -             -                            -           72



* mortality

Mark Creek: netted 23-25 June
Happy Valley Creek: netted 22-25 June
Gustafson Gulch: netted 21, 23 June
Oksrukyik Creek: netted 22-25 June
Goose Green Gulch: netted 25 June


































                                          -24-







Appendix 3. Lengths of fish caught in fyke nets in the Kuparuk River and Smith Creek
      downstream of the Spine Road crossing, 20-21 July 1989.


                                    Length of Fish in Millimeters

Species                  Net 1        Net 2        Net 3         Net 4


Arctic Grayling            34           77           79*           40
                           35           78           79           66
                           35           80           82*          71
                           37           82           85           72
                           38           83           87           73
                           79           86           88*          73
                           79           87          116           74
                           83          110          125           75
                           84          119          127           76
                           86          144          135           77
                           87            -          137           77
                           92            -          181           77
                           113            -          183           78
                                                    253           79
                                                    262           80
                                                    284           81
                                                    287           81
                                                    291           82
                                                    292           82
                                                    306           83
                                                    309           84
                                                     309           84
                                                     338           85
                                                     354           86
                                                     373           87
                                                     434           91
                                                                  102
                                                                  120
                                                                  125

Ninespine Stickleback      54           72           54*           43 *
                                   -      -          66           47
                                                      67           53
                                                      72*          54
                                                       -  -  -    58
                                                       -  -       61
                                                        -  -  -    63
                                                       -  -  -    66
                                                        -  -  -    67
                                                        -  -  -    68







                                      -25-




Appendix 3 continued.




                                       Length of Fish in Millimeters

Species                    Net 1        Net 2         Net 3         Net 4


Slimy Sculpin                76             -             -           56

Broad Whitefish               -             -          465              -



* fyke net mortality

Locations of nets:
 Net 1 - 1.6 km downstream of the Spine Road crossing
 Net 2 - 2.4 km downstream of the Spine Road crossing
 Net 3 - mouth of Smith Creek, 3.2 km downstream of the Spine Road crossing
 Net4 - 4.8 km downstream of the Spine Road crossing


































                                         -26-