[From the U.S. Government Printing Office, www.gpo.gov]











   Fisheries Investigations of Flooded
  North Slope Gravel Mine Sites, 1989

                by:
          Carl R. Hemming

      Technical Report No. 90-2













              Frank Rue
              Director
           Habitat Division
  Alaska Department of Fish and Game
           P.O. Box 3-2000
        Juneau, Alaska 99802

           September 1990







U.S. DEPARTMENT OF COMMERCE NOAA
COASTAL SERVICES CENTER
2234 SOUTH HOESON AVENUE
CIHARLESTON, SC  29405-2413





  Prpoperty of CSC Library











                   TABLE OF CONTENTS


                                                     Page

List of Tables .......................................... iii

List of Figures .......................................... iv

Acknowledgements ....................................... v

Introduction .1
    Description of New Sites ............................... 1
    Previous Fish Sampling ................................ 3
    Mine Site Rehabilitation ............................... 4

Methods ............................................. 6

Results .............................................. 7
    Sag Site C ........................................ 7
    Ott's Oxbow .15
    Kuparuk Deadarm ................................... 15
    Kuparuk Mine Site D ................................. 22
    Kuparuk Mine Site B (Aanaaliq Lakes) ...................... 22
    Lower Put River .................................... 22

Discussion .27
    Sag Site C ........................................ 27
    Ott's Oxbow ..28
    Kuparuk Deadarm ................................... 29
    Kuparuk Mine Site D . ................................ 30
    Kuparuk Mine Site B (Aanaaliq Lakes) ...................... 31
    Lower Put River .................................... 32

Literature Cited ......................................... 34

Appendix I  Depth profile transects of the lower Putuligayuk
    River, August 1989 .................................. 35

Appendix II Water temperatures at fyke net sites, 1989 ................. 37

Appendix III Fish caught in the lower Putuligayuk River near Put
     27, July 27-30, 1988 .................................. 38









                             LIST OF TABLES

                                                                       Page

1.     Number of fish captured, by species, at three fyke net
      locations in Sag Site C, 1989 ............................. 9

2.     Growth rate of recaptured arctic grayling, Sag Site C, July-
      August 1989 ....................................... 14

3.     Number of fish captured, by species, in fyke nets at Ott's
      Oxbow, lower Put River and Aanaaliq Lakes, 1989 ................ 17

4.     Number of fish captured, by species, at three fyke net
      locations in Kuparuk Deadarm Reservoirs 5 and 6, 1989 ............. 20

5.     Limnological sampling in the lower Putuligayuk River near
      Put River Mine Site 27, 17 May 1989 ........................ 26









                           LIST OF FIGURES


                                                                      Page

1.    Map of Prudhoe Bay-Kuparuk oilfields and locations of
      flooded gravel mine sites sampled in 1989 ..................... 2

2.     Locations of fyke nets for fish sampling in Sag Site C, July
      and August 1989 .................................... 10

3.     Length frequency distribution of round whitefish captured
      in Sag Site C, July and August 1989 ......................... 11

4.     Length frequency distribution of arctic grayling captured in
      Sag Site C, July and August 1989 .......................... 12

5.     Location of fyke net for fish sampling in Ott's Oxbow, July
      and August 1989 .................................... 16

6.     Length frequency distribution of arctic grayling captured in
      Ott's Oxbow, August 1989 .............................. 18

7.     Locations of fyke nets for fish sampling in the Kuparuk
      Deadarm, July 1989 ................................... 19

8.     Length frequency distribution of arctic grayling captured in
      the Kuparuk Deadarm, July 1989 ........................... 21

9.     Locations of gill nets for fish sampling in Kuparuk Mine
      Site D, August 1989 .................................. 23

10.   Lower Put River water quality and fish sampling locations,
      1989 ............................................ 25





















                                     iv









                           ACKNOWLEDGEMENTS


Roger Post, Jack Winters, Dr. Alvin Ott, and Dr. Phyllis Weber-Scannell of the Region
III Habitat Division assisted with field data collection. Constructive review comments
were provided by Dr. Alvin Ott and Dr. Phyllis Weber-Scannell. Nancy Ihlenfeldt typed
several drafts of this report.

Financial assistance for this report was provided from the Kuparuk River Unit and the
Prudhoe Bay Unit through a grant to the ADF&G Habitat Division. Steve Taylor of BP
Exploration and Mike Joyce of ARCO Alaska Inc. helped in supporting our effort to
obtain the grant.  Financial assistance was also provided by the Alaska Coastal
Management Program through the Coastal Zone Management Act of 1972, as amended,
and administered by the Office of Ocean and Coastal Resource Management, National
Oceanic and Atmospheric Administration.






































                                          v











Introduction


As part of an ongoing program to monitor the use of flooded gravel mine sites by fish, we

continued and expanded fyke net and gill net sampling in 1989. This annual progress

report on the gravel mine site project presents information on the species composition

and length-frequency distribution of fish captured at six flooded gravel mine sites in the

Prudhoe Bay and Kuparuk oilfield development areas. Sites sampled in 1989 were Sag

Site C, Kuparuk Mine Site D, Kuparuk Mine Site B (Aanaaliq Lakes), Kuparuk Deadarm

Reservoirs 5 and 6, the Sagavanirktok (Sag) River Bypass Road Oxbow (Ott's Oxbow),

and the lower Putuligayuk (Put) River (Figure 1). We added the lower Put River and

Ott's Oxbow to the fish sampling program in 1988 and 1989 respectively. This progress

report contains water quality and bathymetric data for the lower Put River and descriptive

information on the Oxbow site. Earlier progress reports (Hemming 1988, Hemming et al.

1989), present background information on the other four sites.


The objective of the fish sampling program is to provide information on the seasonal use

of several different habitat types resulting from North Slope gravel extraction activities.

This information will guide habitat enhancement of gravel mine sites with the goal of

increasing biological productivity and use by fish and wildlife.


Descriotion of New Sites


The lower Put River is a 9.5 ha pool resulting from gravel extraction operations during

 the early development of the Prudhoe Bay oilfield. This stream reach has a maximum

depth of 4.3 m and a mean depth of 3.8 m (see Appendix I for data from fathometer

 transects). The site is 3.2 km upstream of Prudhoe Bay within estuarine influence. Put

 27, a 15.9 ha deep mined gravel site, is next to the lower Put River. A 115 m buffer strip

 with a gravel flood control berm separates the lower Put River from the Put 27 Mine Site.



                                          -1-






















                                                    Beau fort Sea
Simpson









     Mine~~~~~~~~ S                                   it PRUCHOE















             mine~~~~~~~~~~~~~~~~~~~~xo so ites sape  i99






                                        -2-rFlod










Habitat rehabilitation recommended for this site is a channel connection between the Put

River and the Put 27 Mine Site.


Ott's Oxbow is a 6.9 ha, shallow, back-water area of the Sag River. Parallel, shallow

scraping of riverine gravel deposits formed the site. This gravel extraction technique

created a quiet water zone with a depth of less than 1.5 m. Several small islands and spits

result from irregularities in the depth of material removal. A gravel roadway partially

isolates this back-water habitat from active channels of the Sag River. A single 48" thick

walled pipe downstream of the site provides the only connection to active channels of the

river. The bottom material is soft, fine sand or silt. Ott's Oxbow is upstream of the

ARCO Sag River bridge, on the west side of the river near the Prudhoe Bay Operations

Center. Gravel removal occurred in the mid- 1970's at this site.


Previous Fish Samnlini


In August 1986, we initiated fish sampling at Sag Site C, Aanaaliq Lakes, and Kuparuk

Mine Site D and we continued the sampling at selected sites in 1987 and 1988. Fish were

sampled with graduated mesh gill nets and baited minnow traps in 1986. In April 1987,

we set gill nets beneath the ice at Sag Site C and Kuparuk Mine Site D. In July 1987 we

sampled Sag Site C, Aanaaliq Lakes, and Kuparuk Mine Site D with gill nets and

minnow traps. In August 1987 we sampled Kuparuk Deadarm Reservoirs 5 and 6 with

 gill nets. In mid-May 1988 we set a gill net under the ice at Sag Site C. With the

 exception of the under ice sampling periods, we captured fish in each of the flooded

 gravel mine sites sampled, but there were differences in the species composition and the

 catch per unit effort at the various sites. Hemming (1988) and Hemming et al. (1989)

 reported that species richness and relative abundance of fish was greatest in the flooded

 mine sites located in the floodplains of large river systems, such as the Sag and Kuparuk

 rivers. Large river systems provide fish overwintering habitat. Sites connected to small




                                           -3-










tundra streams draining directly to the Beaufort Sea were colonized by ninespine

stickleback (Pungitius pungitus), broad whitefish (Coregonus nasus), and least cisco

(Coregonus sardinella). We failed to capture freshwater fish in the sites connected to

small tundra streams (Hemming 1988).


In 1988, we used fyke nets in a mark-recapture estimate of arctic grayling abundance in

Sag Site C. The estimated abundance of arctic grayling (Thymallus arcticus) >80 mm FL

was 229+16 (standard error = 8; 95% confidence interval). The estimated density of

arctic grayling >80 mm FL was 14.8 grayling per ha in the 15.5 ha site. During the two

sampling periods in July and August 1988, we captured seven species of fish in fyke nets

fished at up to four locations in Sag Site C. Arctic grayling was the most frequently

captured species. Of the 1,636 fish captured, including recaptures, 60% were arctic

grayling. Round whitefish (Prosopium cylindraceum) and broad whitefish made up 30%

and 8% of the catch respectively, while the remaining 2% consisted of Dolly Varden char

(Salvelinus malma), burbot (Lota iota), slimy sculpin (Cottus cognatus), and ninespine

stickleback (Hemming et al. 1989).


Mine Site Rehabilitation


Recent oil industry habitat enhancement and gravel extraction projects altered the

configuration of three sites investigated during the 1989 open water season. ARCO

Alaska, Inc. completed a habitat enhancement project at Sag Site C in the fall of 1987.

Project personnel removed 183 m of the gravel perimeter berm to allow flooding of the

area on the west side of the site. ARCO Alaska, Inc. also excavated floodplain gravels

outside of the berm to a depth of 0.6 to 1.2 m below the water surface elevation of Sag

Site C. These excavations provided 2.0 ha of shallow water habitat.


In May 1989, ARCO Alaska, Inc. completed their second mine site habitat enhancement

project. This project involved excavating an 18 m x 24 m inlet channel to a depth of 1.8



                                          -4-










mn connecting Aanaaliq Lakes to an adjacent tundra stream (East Creek). An island was

formed by cutting two 14 m x 24 m channels through the overburden dike that separated

two lake basins. Rehabilitation of Aanaaliq Lakes provided a permanent connection

between the stream and the lakes and allowed fish access to both the deep water lakes

and the tundra stream. After rehabilitation, we transplanted 210 arctic grayling to the site

from the Sag River. We hope to establish a reproducing population of grayling in the

lake and tundra stream complex.


BP Exploration excavated an expansion to Kuparuk Deadarm Reservoir 5 in the winter of

1988/ 1989 to provide gravel for a new drill site pad and access road. Spring high water

flooded the excavation creating a 6.2 ha bay estimated to be less than 2.5 m in depth. A

large overburden dike breached by a deeply notched channel separates the expansion area

from the older portion of Deadarm Reservoir 5. The two areas are also connected during

high water at an excavation south of the overburden pile.


In addition to the man-made changes in the gravel mine sites, natural events such as

spring flooding, thawing of adjacent ice rich soils and wind driven wave erosion altered

the sites over time. For example, in early June 1989, spring flood waters in the Sag River

built up behind the Sag River causeway road resulting in a roadway failure. Gravel from

the road failure deposited on top of the ice in Sag Site C, covering an estimated area of 5

ha. High water in the Sag River floodplain restricted through a culvert crossing in the

causeway redistributed gravel in the shallows excavated for habitat enhancement. Gravel

 filled the shallows and reduced the littoral habitat to 0.3 ha. The outlet channel expanded

 during the spring flood as 0.2 ha of shallow water developed. Spring flood waters also

 carried willow branches and other small woody debris and deposited this material along

 the shore and in the shallows near the outlet. Tundra subsidence occurred along the east

 side of the site. The direction of surface flow changed in a wetland area near the




                                           -5-










northeast corner of the site and a small drainage developed that flowed into Sag Site C in

August 1989.


Methods


Fyke nets 3.7 m in length with two 1.2 m square entrance frames, five hoops and a 1.8 m

cod end captured fish at five flooded gravel mine sites in the Prudhoe Bay and Kuparuk

oilfield areas. Net wings measuring 1.2 m by 7.6 m were attached to the first entrance

frame. We set the nets perpendicular to shore with a 30 m center lead attached to the

entrance frames to divert fish into the net. The water depth at each net site governed the

amount of center lead deployed. We emptied fish captured at each fyke net into a

floating holding pen. Each fish was identified, examined for tags, and the fork length

was measured to the nearest millimeter. At some net sites when more than 20 fish of the

same species and size class were captured, we sub-sampled the catch to verify the size

class. The remainder of fish in that size class were counted and released. At Sag Site C

and the Kuparuk Deadarm where the nets were fished on consecutive days, we used

caudal fin clips to identify recaptured fish.


At Kuparuk Mine Site D, we fished 38.1 x 1.8 m graduated mesh gill nets with five 7.6 m

panels having bar mesh sizes of 1.3, 2.5, 3.8, 5.1, and 6.4 cm. One net fished at Kuparuk

Mine Site D was a floater while the remaining nets were sinkers. In the lower Put River

we fished both multifilament and monofilament graduated mesh gill nets under the ice.

These nets measured 9.1 x 1.8 m and had three 3.0 m panels with mesh sizes of 1.3, 1.9,

and 2.5 cm.

In late spring we collected water quality information in the lower Put River. Holes were

drilled through the ice with a gasoline powered ice auger and water samples were

collected just below the ice and at one meter intervals through the water column with a

Van Dom water sampler. We measured the water temperature, dissolved oxygen



                                          -6-










concentration, and salinity concentration of the water samples collected at each depth.

Water temperatures were measured in the Van Dom bottle with a mercury thermometer

and a digital thermometer to check calibration. A Yellow Springs Instrument (YSI)

Model 33, temperature, conductivity, and salinity meter was used to measure the salinity

concentration of samples collected with the Van Dorn bottle. We calibrated the meter

readings with salinity standards of 1, 5, and 15 ppt. Dissolved oxygen concentrations

were determined with the azide modification of the Winkler procedure on 300 ml

prepared samples using a digital titrator.


We gathered depth profile data at the lower Put River with an electronic fathometer.

Depth soundings were taken at 10 second intervals as the boat was operated at a constant

slow speed along an identified transect line. We recorded the depth soundings in feet and

later converted these measurements to metric equivalents.


In July 1988, we used floy tags to mark 37 arctic grayling in Sag Site C. These tagged

fish provided an opportunity to obtain recapture information in 1989. We also measured

water temperatures at each fyke net station with a hand held mercury thermometer

(Appendix II). The area estimates presented in this report were determined from aerial

photographs (scale 1" = 500 ft) using computer assisted, digitizing equipment.


Results


 Sag Site C


The relative abundance and species composition of fish captured in Sag Site C varied

 considerably between sample periods in mid-July and late August. Arctic grayling,

 burbot, and ninespine stickleback numbers increased during the August sampling period

 while round whitefish numbers showed little change between sample periods. We

 captured few broad whitefish, Dolly Varden char, and slimy sculpin. Broad whitefish



                                          -7-










were found in both sample periods and slimy sculpin and Dolly Varden char were

captured only in July. A single 176 mm least cisco was captured in August (Table 1).


We set fyke nets in the shallows near the inlet, off the access ramp, and in the outlet

channel on July 17-19 and August 23-24 (Figure 2). During the two day sample period in

July, we captured 378 fish. The most frequently captured species was round whitefish,

accounting for 61% (n = 233) of the catch. Twenty eight percent (n = 108) of the July

catch were arctic grayling. The remaining species, ranked in decreasing order of

abundance, was broad whitefish, ninespine stickleback, Dolly Varden char, burbot, and

slimy sculpin (Table 1). The most productive net location during the July sample period

was the outlet channel with 50% (n = 191) of the catch. The access ramp location

accounted for 36% (n = 137) of the catch, and the inlet shallows produced only 14% (n =

51).


We captured juvenile arctic grayling ranging from 40-160 mm and juvenile round

whitefish ranging from 40-120 mm more frequently than other species and size classes of

fish during the July sample period. A dominant size mode in the length frequency

distribution of round whitefish appeared in the 60-79 mm size class (Figure 3). We

captured only three round whitefish that exceeded 200 mm in July.


We set three fyke nets for 16 hours on August 23-24. The most abundant species

captured during the second sample period was ninespine stickleback. We captured an

 estimated 15,900 stickleback with the majority of the sticklebacks occurring at the outlet

 channel net site (Table 1). In July and August 1988 and July 1989, fyke nets captured

 fewer than 10 ninespine stickleback from Sag Site C. Arctic grayling numbers also

 increased in August 1989: 1,030 grayling were captured, compared to the 108 captured

 in July 1989 (Table 1); we found 94% (n = 967) of the grayling were 40-59 mm (Figure

 4). Previous investigations of arctic grayling on the North Slope identified individuals in



                                          -8-












Table 1. Number of fish captured, by species, at three fyke net locations in Sag Site C,
         1989.





                                                                                                   Combined
           Arctic     Broad                  Dolly       Least      Ninespine  Round        Slimy      Species**
           Grayling   Whitefish   Burbot     Varden      Cisco      Stickleback Whitefish  Sculpin     Total

July 18
Net 1        3           4           0          0           0           0          19          1          27
Net 2       27           8           1          2           0           0         78           0         116
Net3        30           4           0          3           0           1        106           0         143
TOTAL        60          16           1          5           0           1        203           1        286

July 19
Net 1        17          0           0          0           0           6          0           0          17
 Net 2        9           3           1          0           0           0          8           0          21
 Net 3       22           0           0          1           0           2         22           0          45
TOTAL        48           3           1          1           0          8          30           0         83

August 24
 Net 1       91           1           2          0           0       350*          69           0         163
 Net 2      557           8          32          0           0        550*         122          0         719
 Net 3      382           0           5          0           1    15,000*          20           0         408
TOTAL    1,030            9         39           0           1    15,900*         211           0       1,290




Location of fyke nets:
  1 = shallow water littoral zone
  2 = access ramp
  3 = outlet channel

 * ninespine stickleback numbers were estimated
 ** ninespine stickleback excluded from total























                                                       -9-





















            pipeline



                                                         4ndra
                                                        drainage











                  '0     2                   /
        culverts                                      T


                   Y                              o meters


   Fyke net locations:
   1. inlet shallows
   2. access ramp
   3. outlet channel










Figure 2. Locations of fyke nets for fish sampling in Sag Site C, July and August 1989.










                                 -10-




















               July  17-19                           August  23-24
               N=233                                 N=211
     1000 _









 .4 -
    2   0 -
 0












     0.1 0
             0  20 40 60 80 100120 140 160 180 200220240280 280 300 320 340 380380400

                                       Length mm












Figure 3. Length frequency distribution of round whitefish captured in Sag Site C, July
       and August 1989.











                                         -11-
















             July  17-19                        =   August  23-24
             N=79                                    N= 1030
     1000_


      100 -
 oT


    100
 o 


        L !' ii
    0.1

           0  20  40  60  80  100 120 140 180 180 200 220 240 260 280 300 320

                                Length mm








Figure 4. Length frequency distribution of Arctic grayling captured in Sag Site C, July
     and August 1989.








                                 -12-










this size class as young-of-the-year fish (deBruyn and McCart 1974, Bendock 1979).

The distribution of lengths of grayling captured in the July and August samples were

compared using the Kolomogrov Smirnov (KS) test to determine if the two samples were

from the same statistical population. The cumulative probability distribution of fork

lengths of grayling captured in July was significantly different from the August sample (p

<0.01, d = 0.8772); therefore, we concluded the samples were from different populations.

A plot of the length-frequency distribution of the two samples (Figure 3) indicates that

the differences are due to the young-of-the-year grayling found in the August sample.


The distribution of the August catch among the three net site locations varied from the

pattern observed during the July sample period. In August 54% (n = 557) of the arctic

grayling were captured at the access ramp net while the outlet channel net contained 37%

(n = 382) and the inlet shallows accounted for 9% (n = 91).


The number of burbot increased from two captured in July to 39 in August. The burbot

captured in August ranged from 52-145 mm and averaged 68 mm (SD 14.3 mm). A

typical length for burbot in interior Alaska at age 1+ is 136 mm (Chen 1969); therefore, it

is likely that most of the burbot were young-of-the-year fish.


In the July and August sample periods we recaptured 2 of the 37 arctic grayling that were

marked with floy tags in July 1988. Low water levels in the Sag River kept the site

isolated from active channels of the Sag River until June 1989. The recaptured fish had

slow growth rates between the time of tagging and the time of recapture (Table 2) when

compared to the arctic grayling in Aanaaliq Lakes. The Aanaaliq Lakes arctic grayling

were marked in late June 1989 and recaptured in August and early October 1989.









                                          -13-










Table 2. Growth rate of recaptured arctic grayling, Sag Site C, July-August 1989.




Tag    Date      Fork         Date         Fork        Total    Growth        Est. Growth
No.    Marked  Length         Recaptured  Length       Days      (mm)         (mm/day)
                 (mm)                     (mm)


509    7/13/88    304           7/18/89     317        371          13          0.035

509    7/13/88    304           8/24/89     323        408          19          0.046

552    7/14/88    305           7/18/89     302        370          -3         -0.008













































                                              -14-










Ott's Oxbow


We fished a single fyke net in Ott's Oxbow on July 17-18, at the north end of the site

with 30 m of center lead (Figure 5). We captured 129 fish, including 114 small broad

whitefish, 13 arctic grayling, 1 ninespine stickleback, and 1 round whitefish (Table 3).

The broad whitefish ranged from 43-55 mm (mean = 48.5 mm, SD = 2.6) and the 13

arctic grayling ranged from 138-350 mm (mean = 229.6 mm, SD = 62.5).


We fished the net at the same location on August 22-23 for a 27 hour period. We

captured 559 fish including 327 arctic grayling, 150 ninespine stickleback, 50 round

whitefish, 28 burbot and 4 broad whitefish. Juvenile arctic grayling were captured most

frequently as 83% (n = 273) of the arctic grayling catch was in the 40-59 mm size class

(Figure 6). A subsample of 15 round whitefish ranged from 60-77 mm (mean = 66.9, SD

= 5.0), and we judged the 50 round whitefish to be in the 60-79 mm size class. A

subsample of 10 burbot ranged from 60-69 mm (mean = 65.3 mm, SD = 3.3)and we

judged the 28 burbot to be in the 60-70 mm size class. The 4 broad whitefish captured

ranged from 87-93 mm.


KuDaruk Deadarm


We set three fyke nets in Kuparuk Deadarm Reservoirs 5 and 6 on July 18-21. The nets

fished at the south end of the expansion area of Reservoir 5, at the south end of Reservoir

5, and on the west side of Reservoir 6 (Figure 7). The three nets captured 127 fish,

including 72 grayling, 52 ninespine stickleback, 2 slimy sculpin, and a single burbot

(Table 4). Juvenile arctic grayling in the 100-160 mm size range occurred most

frequently in the sample catch (Figure 8). The Reservoir 6 net captured 65 fish, while the

expansion area of Reservoir 5 produced 51, and the remaining 12 were captured in

 Reservoir 5. We used only 10 m of center lead in Reservoir 5, while 25 m of center lead




                                         -15-











                                     Sag River C











                              K                             5
                           culver /,






                                                     0         150
                                                        meters
                           \'s %    l\(    I Fyke net 
                                                        location








                    ARCO airstrip












Figure 5. Location of fyke net for fish sampling in Ott's Oxbow, July and August 1989.




                                 -16-











Table 3. Number of fish captued, by species, in fyke nets at Ott's Oxbow, lower Put River,
        and Aanaaliq Lakes, 1989.




                                                                                   Combined
                Arctic   Broad            Fourhom Least   Ninespine* Round         Species**
                Grayling Whitefish Burbot Sculpin  Cisco  Stickleback Whitefish  Total

July 18
 Ott's Oxbow      13       114       0       0        0         1         1          129

August 23
 Ott's Oxbow     327         4      28       0        0      150*        52         411

July 21
 Put River         0         0       0       3         0       14         0            3

August 24
 Put R. (2 nets)   0         1       0       3        0      400*         0            4

August 23
 Aanaaliq Lakes:
  Area A            0        0       0       0         0  10,000*         0            0
  Area B            1**              0       0         1  10,000*         0            2

August 24
 Aanaaliq Lakes:
  Area A            0        0       0       0         0  18,000*         0            0
  Area B            1***     1       0       0         2   8,000*         0            4


 * Ninespine stickleback numbers estimated
 ** Ninespine stickleback excluded from total
 *** Arctic grayling transplanted to site





















                                               -17-





















              August  23-24
              N=327
     1000 


 (D
  o       -
      100



 -
       1C-






 E


       0.1
            0  20  40  60  80 100 120 140 160 180 200 220 240 260 280 300 320 340

                                   Length mm













Figure 6. Length frequency distribution of arctic grayling captured in Ott's Oxbow,
      August 1989.










                                      -18-












                                             Reservoir 6



















                    Reservoir 5                      N



                                                      150
              %n ~~ ~ j~~~ 'gmeters
                                             Fyke net i
                                             location
  Reservoir 5 
  expansion 






              o                              Reservoir 4





Figure 7. Locations of fyke nets for fish sampling in Kuparuk Deadarm, July 1989.











                             -19-









Table 4. Number of fish captured, by species, at three fyke net locations in the Kuparuk
       Deadarm Reservoirs, 1989.




                                                                       Combined
              Arctic                      Ninespine    Slimy            Species
              Grayling      Burbot        Stickleback   Sculpin        Total


July 19
 KDR 5           6            0               0             0              6
 KDR 5 exp.   24              0               0             0             24
 KDR 6           8            0              31             0             39
TOTAL           38            0              31             0             69

July 20
 KDR 5           2            1               2             0              5
 KDR 5 exp.    8              0               0             0              8
 KDR 6           7            0               8             0             15
TOTAL           17            1              10             0             28

July 21
 KDR 5           1            0               0             0              1
 KDR 5 exp.   14              0              40             0             18
 KDR 6           3            0               6             2             11
TOTAL           18            0              10             2             30



KDR = Kuparuk Deadarm Reservoir
























                                          -20-



















               July  17-19
               N=63
       30





 t
       2  20 -















                                    Length mm












Figure 8. Length frequency distribution of Arctic grayling captured in the Kuparuk
      Deadarm, July 1989.










was used in the expansion area and 30 m of lead was used in Reservoir 6. The Reservoir

5 expansion area produced 63% (n = 46) of the grayling captured.


Kuraruk Mine Site D


We set four variable mesh gill nets in Kuparuk Mine Site D for 17 hours on August 22-

23, 1989. Sinking nets fished at the access ramp, in the inlet channel, and at the

northwest comer of the site, while a floating gill net fished at the northeast corner (Figure

9). We captured a single 191 mm least cisco at the access ramp.


Aanaalia Lakes


We set two fyke nets in Aanaaliq Lakes on August 22-24, 1989. The nets fished at the

northwest comer of the site and at the inlet channel. We used 15 m of center lead at each

location. Each net produced a large catch of ninespine stickleback. We estimated the

number of stickleback in each net based on visual examination of the net content. The

estimates ranged from 8,000 to 18,000 at each site (Table 3). The trap at the inlet

channel captured 3 least cisco, 2 arctic grayling, and 1 broad whitefish in addition to the

stickleback. The net at the northwest comer of the site failed to capture species other

than stickleback. The three least cisco ranged from 184-196 mm and the broad whitefish

was 240 mm.


Lower Put River


We fished two variable mesh gill nets beneath the ice of the lower Put River on May 17-

 18, 1989. On May 17 we collected water quality information at the gill net sample sites.

Open water fish sampling was conducted with fyke traps on July 20-21 and August 23-

 24, 1989.






                                          -22-

















                    A > ~~~~~settling
      Charlie pond
      Creek overburden pile



  eroded areai

        /XX*
         y/ |  overburden
        ! ~berm


                                  //N

     I  /  (                             A            0 e        150

                                  \m  ~meters
                                    net location U
                       culverts







Figure 9. Locations of gill nets for fish sampling in Kuparuk Mine Site D, August 1989.









                       -23-










We set gill nets in the Put River at locations 400 m and 600 m downstream of Drill Site

15 access road crossing (Figure 10). The downstream net produced a single 260 mm

round whitefish. An analysis of the stomach content of the round whitefish indicated

recent feeding as the stomach was full. Identifiable food items included midge and

caddisfly larvae. The midge larvae were numerous while only two caddisfly larvae were

identified.


We found similar water temperatures, dissolved oxygen concentration, and salinity at

each depth sampled in the lower Put River (Table 5). Both sample sites had the same

pattern of uniform water quality characteristics at each depth sampled. The water

temperature measurements indicated a slight super-cooling effect (-0.5ï¿½C) at all depths.

The dissolved oxygen concentrations ranged from 6.4 mg/L to 6.9 mg/L. The salinity

concentrations were 6.7 ppt. The water depth was 4.5 m at both sample locations. The

ice depth was 2.1 m at the downstream sample site which was windblown and 1.7 m at

the upstream site where more snow cover was present.


In July, we set a single fyke net 250 m downstream of Drill Site 15 access road crossing

of the Put River. The net captured 14 ninespine stickleback and 3 fourhorn sculpin

(Myoxocephalus quadricornis) (Table 3). In August we set two nets, they fished at

locations 250 m and 800 m downstream of Drill Site 15 access road. High winds rolled

the upstream trap onto its side and it captured only two ninespine stickleback. We set the

downstream net partially blocking the stream channel at a location where the pool

 narrows. This net captured one adult broad whitefish (475 mm), three fourhorn sculpin,

 and an estimated 400 ninespine stickleback (Table 3).










                                         -24-





















      o           150                                       /
         meters
      a fyke net       /       Lower Put River
        location     ,/  
      A. water sample
        and gill net               A&  access
        location                                ramp



    Drill Site 15          //
    Access Road  /  '                                    control berm



     _/< '/culverts \                        I  access
                                              ramp
                                                  Put 27










Figure 10. Lower Put River water quality and fish sampling locations, 1989.










                                     -25-










Table 5. Limnological sampling in the lower Putuligayuk River near Put River Mine
        Site 27, 17 May 1989.




             Total         Ice       Sampling                  Dissolved
             Depth      Thickness      Depth    Temperature   Oxygen          Salinity
Location       m             m            m          ï¿½C           mg/L          ppt


Site A*        4.5          2.1
                                        2           -0.5          6.5          6.7
                                        3           -0.5          6.9          6.7
                                        4           -0.5          6.5          6.7

Site B**       4.5          1.7
                                        2           -0.5          6.4          6.7
                                        3           -0.5          6.9          6.7
                                        4           -0.5          6.6          6.7



* 600 m downstream of Drill Site 15 road crossing
** 400 m downstream of Drill Site 15 road crossing


































                                               -26-










Discussion


Sag Site C


High water levels in the Sag River kept inlet and outlet channel connections open through

most of the 1989 open water season. These highwater channel connections provide a

mechanism for the movement of fish between Sag Site C and the Sag River system. The

outlet channel flowed during both the July and the August sampling periods while the

inlet channel became discontinuous just prior to the August sampling period.


During the 1988 open water season, Sag Site C did not have active inlet or outlet

connections to the Sag River system, except for a brief period during the spring flood in

early June. In mid-July, we captured and marked 37 large grayling (>200 mm) with floy

tags. Between the time of marking in July and the spring flood in June 1989, Sag Site C

was isolated from the Sag River system. In July 1989, we recaptured two tagged grayling

demonstrating overwinter use and winter survival in Sag Site C.


The arctic grayling recaptured in Sag Site C had slower growth rates than those

recaptured in Aanaaliq Lakes.  Our earlier progress report (Hemming et al. 1989)

provides data that compares Aanaaliq Lakes to Sag Site C in phytoplankton production

and zooplankton density. We found Aanaaliq Lakes ranked higher than Sag Site C in

both areas. The arctic grayling growth rate data also supports the conclusion that

Aanaaliq Lakes provides more productive aquatic habitat than Sag Site C.


We captured only seven grayling >200 mm in 1989. The absence of large grayling in the

sample catch indicates that tagged grayling may have moved into the Sag River during

the spring flood as high water provided a mechanism for dispersal. Out-migration to the

Sag River could occur as fish seek suitable spawning areas or more productive summer

rearing habitats.



                                         -27-










Rainfall events caused severe flooding in headwater areas of the Sag River between the

July and August sample periods. The USGS water stage recorder located near Pump

Station 3 measured a peak, late summer, mean daily discharge of 9,040 cfs on August 3,

1989 (USGS 1989). It is likely that this high water event moved juvenile fish from

rearing areas and transported them downstream. The abundance of young-of-the-year

grayling, burbot fry, and ninespine stickleback showed a marked increase in the fyke net

catches during the late August sample period. It is possible that the flood event that

peaked on August 3 transported the small stickleback, grayling, and burbot downstream

into the lower Sag River resulting in the increased rate of catch. Sag Site C remained

connected to the Sag River by an outlet channel during the August sample period. Fish

found in the site in late summer remained in the deep water mine site when access to

riverine habitats was available. It is possible that these fish selected Sag Site C as an

overwintering area.


Ott's Oxbow

Ott's Oxbow is a shallow, warm, backwater area that is best characterized as a summer

rearing habitat. It is unlikely that this site provides suitable fish overwintering habitat;

therefore, use by fish depends upon seasonal movements between the site and the Sag

 River system. These movements might include those from overwintering areas in the

 river after spring break-up and out-migration to deep overwintering areas in the river late

 in the open water season.


 Ott's Oxbow remained connected to the Sag River system during both the July and

 August sampling periods. The species composition and relative abundance of fish

 captured varied considerably between sample periods. It is likely that an exchange of

 fish occurred between the site and the Sag River system. Seasonal movements of fish

 into and out of Ott's Oxbow are notable because the site is connected to the river system



                                          -28-










by a single 48" culvert placed through a gravel road fill that partially isolates the site

from the river system (Figure 5).


During July, juvenile broad whitefish were the most abundant species in the sample

catch. It is possible that small broad whitefish transported from the Sag River

overwintering areas during the spring flood moved into the shallow backwater area of

Ott's Oxbow seeking conditions favorable for summer rearing.


In August, broad whitefish numbers decreased in the fyke net catch indicating that the

juvenile broad whitefish moved out of the site and into the Sag River system. The timing

of this movement out of Ott's Oxbow corresponds with the appearance of young-of-the-

year broad whitefish at offshore fyke net locations. These data suggest that young-of-

the-year broad whitefish occupy backwater rearing habitats in the Sag River until late

summer when a downstream movement occurs. High-water events in late summer may

be a factor in the downstream movement (Dillinger and Gallaway 1989). The numbers

of young-of-the-year grayling, burbot fry, and ninespine stickleback increased in the

August sample catch. This pattern is similar to that found in the 1989 fyke net catch at

Sag Site C. In addition round whitefish numbers increased in the August catch. It is

possible that the August high-water event in the Sag River transported ninespine

stickleback, burbot, grayling, and round whitefish downstream into the lower Sag River

resulting in an increase in the abundance of these species in downstream rearing areas

 such as Ott's Oxbow. It is likely that these fish out-migrate from shallow rearing areas to

deeper sections of the river suitable for overwintering with the onset of cooler weather.


 Kuuaruk Deadarm


 During the July sample period low water levels in the Kuparuk River isolated the

 Deadarm Reservoir complex from the river system.   We  observed a high water




                                         -29-










connection between the Kuparuk Deadarm Reservoirs and the Kuparuk River during the

spring flood and in August following late summer rainfall events.


The species diversity and relative abundance of fish captured in Kuparuk Deadarm

Reservoirs 5 and 6 were lower than that found in the Sag River sites sampled in 1989.

Fyke nets fished in the lower Kuparuk River to obtain grayling for disease screening also

caught fewer fish than those fished in the Sag River sites in 1989. The results of the fyke

net sampling suggests that fewer fish are available in the lower Kuparuk River for

dispersal into the Kuparuk Deadarm Reservoirs than that found in the lower Sag River.


In August 1987, we captured 10 large (368-425 mm) arctic cisco (Coregonus autumnalis)

in gill nets fished in the Kuparuk Deadarm Reservoirs (Hemming 1988). Arctic cisco

were not captured in the July 1989 fyke net catch. It is possible that the large arctic cisco

out-migrated from the Kuparuk Deadarm to the Kuparuk River during high water. Data

obtained from the Colville River commercial fishery and from several oil development

related fish monitoring studies indicate that arctic cisco in the size class captured in the

Kuparuk Deadarm are likely to be mature. Mature arctic cisco are captured infrequently

in the Colville River and Sag River area. The theory has been advanced that mature

arctic cisco return to the Mckenzie River in Canada to spawn (Gallaway et al. 1983).

Therefore, it is possible that the large arctic cisco found in the Kuparuk Deadarm in 1987

moved out of the site upon reaching sexual maturity and migrated to the Mckenzie River

system to spawn. It is also possible that arctic cisco are present in the deeper areas of the

Kuparuk Deadarm Reservoir that are not effectively sampled with fyke nets fished in the

shallows.


Kuparuk Mine Site D


We captured least cisco in Kuparuk Mine Site D during gill net sampling periods in

August 1986 and August 1989. On August 21, 1986 we captured a single 280 mm least



                                         -30-










cisco in a gill net set at the access ramp to the site. In 1989 a gill net set at the same

location captured a single 191 mm least cisco. Graduated mesh gill nets fished at the

access ramp and other locations in Kuparuk Mine Site D in August 1986, April 1987,

July 1987, and August 1989 failed to capture fish other than the two least cisco.


Kuparuk Mine Site D is connected to the Ugnuravik River system which drains into the

Beaufort Sea.  It is unlikely that the Ugnuravik River system provides suitable

overwintering habitat; therefore, the least cisco found in Mine Site D originated in other

drainages (e.g., Colville River) and moved into the Ugnuravik River from the Beaufort

Sea. The water levels in the Ugnuravik and other tundra streams in the Kuparuk area

appeared higher in 1989 than observed previously. High water in the Ugnuravik River

may have been a factor in the movement of least cisco into Mine Site D. It is interesting

to note that fyke nets fished in Aanaaliq Lakes and Sag Site C also captured least cisco in

August 1989.


In previous field investigations we observed large concentrations of ninespine stickleback

in the shallow water areas of the inlet channel to Kuparuk Mine Site D and in Charlie

Creek, a tundra stream that is connected to the mine site.  We  also observed

concentrations of stickleback in the Ugnuravik River near Kuparuk Mine Site C and in

East Creek near Aanaaliq Lakes. These observations indicate that the abundance of

ninespine stickleback is greater in Kuparuk area streams connected to flooded mine sites

than in area streams without mine sites (Ott 1989). It is likely that the stickleback

population in these tundra streams has increased in response to the substantial expansion

in available overwintering habitat provided by the deep water areas of the mine sites.


 Aanaalia Lakes


 Fyke nets captured broad whitefish and least cisco in Aanaaliq Lakes in August 1989.

 Broad whitefish were captured in gill nets fished in July 1987 while least cisco had not


                                          -31-










been captured previously. It is likely that the broad whitefish and least cisco found in

Aanaaliq Lakes moved into the site from neighboring drainages (e.g., Colville River)

because East Creek does not provide suitable overwintering habitat. It is not known if

the broad whitefish and least cisco found in Aanaaliq Lakes overwinter there or out-

migrate to the Beaufort Sea and move to other overwintering areas.


We captured thousands of ninespine stickleback in fyke traps fished in August 1989. It is

likely that the number of ninespine stickleback in East Creek and Aanaaliq Lakes

increased in response to the expansion of available overwintering habitat provided by the

mine site.


The grayling captured were part of an experimental fish transplant conducted in June

1989.


Lower Put River


In July 1988, fyke net sampling documented use of the lower Put River by juvenile arctic

char, broad whitefish, ninespine stickleback, and fourhorn sculpin (Appendix III). Fyke

nets fished in the lower Put River in July and August 1989 captured fourhorn sculpin,

ninespine stickleback, and a single adult broad whitefish. The open water fyke net catch

indicates that the lower Put River is a summer rearing habitat for marine and anadromous

fish.


We found estuarine conditions in the lower Put River in May 1989. Stream sections with

 similar water quality characteristics in the lower Sag River and lower Colville River are

 used by overwintering arctic cisco and least cisco (Schmidt 1987, Adams 1987). We

 captured a single round whitefish in the lower Put River in May 1989.


 Habitat enhancement proposed for the Put 27 Mine Site consists of a channel connection

 to the lower Put River. This project will result in a substantial increase in the volume of


                                          -32-










available fish overwintering habitat in the lower Put River.   The  water quality

characteristics of the Put 27 reservoir will depend on the nature of the spring flood event

in the Put River that fills the reservoir. We anticipate that the reservoir will fill with fresh

water initially, with the possibility that an increase in salinity may occur over time,

resulting from storm surge events.












































                                            -33-








                             LITERATURE CITED


Adams, B., and T.C. Cannon. 1987. Overwintering study in 1985 final report for the
      Endicott Environmental Monitoring Program. Vol. 7, Part V. Prepared by
       Envirosphere Co. for U.S. Army Corps of Engineers, Anchorage, Alaska. 33 pp.

Bendock, T.N. 1979. Inventory and cataloging of arctic waters. ADF&G, Fed. Aid in
       fish. Rest. Ann. performance rept. Vol. 20. Project F-9-12, Job G-I-I. Juneau.
       31 pp.

Chen, L.C. 1969. The biology and taxonomy of the burbot, Lota lota leptura, in interior
       Alaska. Biol. Pap. Univ. Alaska 11:1-51.

deBruyn, M., and P. McCart. 1974. Life history of the grayling (Thymallus arcticus) in
       Beaufort Sea drainages in the Yukon Territory. Chap. 2 in P.J. McCart, ed.
       fisheries research associated with proposed gas pipeline routes in Alaska, Yukon,
       and Northwest Territories. Arctic Gas Biol. Rept. Ser. Vol. 15. 39 pp.

Dillinger, R.E., and B.J. Gallaway. 1989. Status of the Sagavanirktok broad whitefish
       (Coregonus nasus) populations with an evaluation of the effects of the Endicott
       Causeway. Chapter 11 in The 1988 Endicott Development Fish Monitoring
       Program: A monograph of project papers, 1988. Compiled by LGL Alaska
       Research Associates Inc. for BP Exploration Alaska, Inc., North Slope Borough.

Gallaway, B.J., W.B. Griffiths, P.C. Craig, W.J. Gazey, and J.W. Helmericks. 1983. An
       assessment of the Colville River stock of arctic cisco migrants from Canada?
       Biol. Pap. Univ. of Alaska, No. 21:4-23.

Hemming, C.R. 1988. Aquatic habitat evaluation of flooded North Slope gravel mine
       sites (1986-1987). Alaska Dept. of Fish and Game, Habitat Division Tech. Rept.
       No 88-1. Juneau. 69 pp.

Hemming, C.R., P.K. Weber, and J.F. Winters. 1989. Limnological and fisheries
       investigations of flooded North Slope gravel mine sites, 1988. Alaska Dept. of
       Fish and Game, Habitat Division Tech. Rept. No 89-1. Juneau. 60 pp.

Ott, A.G. 1989. North Slope oil and gas development cross drainage report. Alaska
       Department of Fish and Game, Habitat Division. 72 pp.

Schmidt, D.R., W.B. Griffiths, and L.R. Martin. 1987. Importance of anadromous fish
       overwintering habitat in the Sagavanirktok River delta, Alaska. Prepared by LGL
       Research Associates, Inc. for Standard Alaska Production Co. and the North
        Slope Borough. 71 pp.

 USGS. 1989. Water Resources Data Alaska Water Year 1989. Provisional data subject
        to revision.








                                         -34-









Appendix I. Depth profile transects in the lower Put River, August 1989.

Location of Transects















          meters

                    N     /A




































                               -35-











Depth Profile Transects







                                A-A Transect


           0-





          -2-


          -3-

     CA.
     a.)  -4-


          -5 -


          -6 -


              0                       325                       650

                                  Distance m








                                 B-B Transect


            0-






           -2 -
      E

           -3 
      a

           -4 -


           -5 -


           -6
                -0                                              132

                                   Distance m






                                       -36-










Appendix II. Water temperatures at fyke net sites, 1989.




Date       Location of Fyke Trap                                 *Temperature


7/18       Ott's Oxbow                                                14.0
7/19       Sag Site C Net Site 1                                      13.5
           Sag Site C Net Site 2                                      10.5
           Sag Site C Net Site 3                                      12.0
           Kuparuk Deadarm Reservoir 5                                11.5
           Kuparuk Deadarm Reservoir 5 expansion                      13.5
           Kuparuk Deadarm Reservoir 6                                14.0
7/20       Kuparuk Deadarm Reservoir 5                                11.0
           Kuparuk Deadarm Reservoir 5 expansion                      13.0
t"        Kuparuk Deadarm Reservoir 6                                 12.5
7/21       Lower Put River                                            11.0
1"         Kuparuk Deadarm Reservoir 5                                10.0
           Kuparuk Deadarm Reservoir 5 expansion                      11.5
        Kuparuk Deadarm Reservoir 6                                    11.0
8/23       Aanaaliq Lakes, north                                      11.5
           Aanaaliq Lakes, south                                      11.0
"         Ott's Oxbow                                                 13.0
8/24       Aanaaliq Lakes, north                                      12.0
           Aanaaliq Lakes, south                                      12.0
           Sag Site C Net Site 1                                      10.0
f"         Sag Site C Net Site 2                                      10.5
           Sag Site C Net Site 3                                      10.0



* measurements to the nearest 0.5'C




















                                         -37-









Appendix III. Fish caught in the lower Putuligayuk River near Put River Mine Site 27,
      July 27-30, 1988.


       LOCATION                    NUMBER & LENGTH OF FISH CAUGHT

                              Ninespine      Arctic         Broad         Fourhorn
                              Stickleback    Char           Whitefish      Sculpin


600 m downstream of Drill
Site 15 road crossing
 July 27                          2              -
 July 28                          37          1(203)+          1(82)+

200 m downstream of Drill
Site 15 road crossing             60*       2(233;102)+    3(422;93;88)+    9(87)+
                                 30*    3(191;203;200)+        1(96)+


+ length of each fish in millimeters
* estimated number































                                        -38-