[From the U.S. Government Printing Office, www.gpo.gov]



                                           NOAA STATUS AND TRENDS
                                                           Mussel Watch Project
                                                          Field Sampling & Logistics
                                                                                         Year IEK


                                                                             The Geochemical and
                                                                              Environmental Research Group
                                                                             Texas A&M Research Foundation




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                       year 9
                                                                                                                                                                                    3
                       (1994)
                                                                                         C 0 er









                                       NOAA
		             NATIONAL STATUS AND TRENDS

                          Mussel Watch Program
               Field Sampling and Logistics Report

                                        Year IX
                                     Prepared by'

                     H.J. Jobling, J.M. Brooks, and R.R. Fay

            The Geochemical and Environmental Research Group
                               Texas A&M University
                                  833 Graham Road
                           College Station, Texas 77845

                                                     Property Of CSC Library


                                       Submitted to

                              U.S. Department of Commerce
                      National Oceanic & Atmospheric Administration
                                 1305 East West Highway
                              Silver Spring, Maryland 20910

                                      October 1994
                              GERG Technical Report 94-134                                                        









                                                            11 ELD SAMPLING AND LOGISTICS
                                                                          TABLE OF CONTENTS
                                                                                                                                                          Page

                       Sampling History               ................................................                                                      1
                       Bivalve Distributional Patterns in the Gulf of Mexi'co                                   .......................................     2
                       Field Logistics           ............................................................................................               5
                       Logistic Considerations                  .............................................................................             16
                       Permits        ...................................................................................................                 10
                       Sampling Gear               ..........................................................................................             .12

                                 Boats      ..............................................................................................                12
                                 Radio Communications                        ....................................................................         12
                                 Safety Considerations                     .......................................................................        12

                       Sample Collection               .....................................................................................              13

                                 Oyster Collections              ............................................................................             13
                                 Sediment Collections                    ........................................................................         13

                       Field Processing            .......................................................................................                13

                                 Salinity and Temperature                          .................................................................      13
                                 Oyster Processing               ............................................................................             14
                                 Sediment Processing                     ........................................................................         18
                                 Sample Handling and Chain-of-Custody                                  ............................................       20

                       Field Sampling Criteria                   ............................................................................             2D

                                 Definition of Stations and Sites                                                                                         20
                                 Bivalve Site Selection Criteria                          ............................................................    22
                                 Sediment Site Selection Criteria                        ........................................................         23

                       Sampling Summary                    .................................................................................              24

                                 Sampling Problems                   .........................................................................            24
                                 Years I and II Sampling Summary                                   ...................................................    25
                                 Year III Sampling Summary                           ...........................................................          26
                                 Year IV Sampling Summary                               ............................................................      29
                                 Year V Sampling Summary                           .............................................................          31
                                 Year VI SamplingSummary                            ............................................................          32
                                 Year VII Sampling Summary                            ..........................................................          33
                                 Year VIII Sampling Summary                                .........................................................      34
                                 Year IX Sampling Summary                           ............................................................          35

                       References          ...............................................................................................                39
                       Gulf of Mexico and Caribbean Site Descriptions                                   ...........................................       40
                       Sample Site Descriptions                    ............................................................         I................. 55
                       Appendix A - Field Station Data                       ................................................................             A-1
                       Appendix B - Final Positions                      .....................................................................            B-1


                                                                                          iii










                                                                      LIST OF ILLUSTRATIONS


                                                                                    FIGURES
                      Figure                                                                                                                           Page

                      1          Sample processing protocol for oyster sites                             .........................................        15
                      2          Sample processing protocol for mussel sites                                .......................................       17
                      3          Sample processing protocol for sediment sites                                     .....................................  19
                      4          Decision criteria for sediment sites                       ....................................................          21
                      5          Location of Gulf of Mexico Sampling Sites                              ..........................................        41
                      6          Location of Caribbean Sampling Sites                            ................................................         42
                      7          Location of Laguna Madre South Bay Sites                                .........................................        43
                      8          Location of South Texas Sites                     ............................................................           44
                      9          Location of Galveston Bay Sites                            ..........................................................    45
                      10         Location of Western Louisiana Sites                              ...................................................     46
                      11         Location of Eastern Louisiana and Mississippi Delta Sites                                           .................... 47
                      12         Location of Mississippi/Alabama Sites                            ...............................................         48
                      13         Location of Western Florida Sites                             .......................................................    49
                      14         Location of Middle Florida Sites                           .........................................................     50
                      15         Location of Tampa Bay Sites                           ..............................................................     51
                      16         Location of South Florida Sites                     ..........................................................           52
                      17         Location of Florida Key Sites                    .............................................................           53
                      18         Location of Puerto Rico Sites                    .............................................................           54


                      Table                                                         TA13LES                                                            Pa
                                                                                                                                                           Z-e

                      1          Collection Methods and Site Accessibility                            .............................................         7
                      2          Sediment and Oyster Sampling, Years I - IX                                  ......................................       27




















                                                                                         iv










                                                   LIST OF SUES WrrH MAPS AND PHOTOGRAPHS

                      Site                                                Description                                                                 Page

                      1          LMSB South Bay, Lower Laguna Madre, TX                                      .....................................     55
                      52         LMPI Port Isabel, Lower Laguna Madre, TX                                    ....................................      61
                      78         LMAC Arroyo Colorado, Lower Laguna Madre, TX                                          ............................    67
                      2          CCN-B        Nueces Bay, Corpus Christi, TX                                 ............................................. 73
                      3          CCIC         Ingleside Cove, Corpus Christi, TX                             ........................................  79
                      53         CCBH         Boat Harbor, Corpus Christi, TX                                ............................................ 85
                      4          ABLR         Long Reef, Aransas Bay, TX                      ..................................................       91
                      54         ABHI         Harbor Island, Aransas Bay, TX                                 ............................................ 97
                      5          CBCR         Copano Reef, Copano, Bay, TX                     ...............................................        103
                      6          MBAR Ayres Reef, Mesquite Bay, TX                             ...............................................        109
                      7          SAPP         Panther Point Reef, San Antonio Bay, TX                            ...............................      115
                      8          SAMP         Mosquito Point, San Antonio Bay, TX                               ..................................... 121
                      9          ESSP         South Pass Reef, Espiritu Santo, TX                            ......................................   1T
                      10         ESBD         Bill Days Reef, Espiritu Santo, TX                             .........................................M
                      11         MBLR         Lavaca River Mouth, Matagorda Bay, TX                              ...............................      M
                      12         MBGP         Gallinipper Point, Matagorda Bay, TX                           ...................................      145
                      13         MBTP         Tres Palacios Bay, Matagorda Bay, TX                               ...................................  151
                      14         MBEM East Matagorda, Matagorda Bay, TX                                      .....................................    157
                      56         MBCB         Carancahua Bay, Matagorda Bay, TX                                 ....................................  163
                      55         MBDI         Dog Island, Matagorda Bay, TX                                  ............................................ 169
                      72         BRCL         Cedar Lake, Brazos River, TX                     ...............................................        175
                      57         BRFS         Freeport Surfside, Brazos River, TX                            ......................................   181
                      15         GBYC         Yacht Club, Galveston Bay, TX                       .............................................       187
                      16         GBTD         Todd's Dump, Galveston Bay, TX                                 .......................................... 193
                      17         GBHR         Hanna Reef, Galveston Bay, TX                                  ............................................ 201
                      18         GBCR         Confederate Reef, Galveston Bay, TX                            .....................................    207
                      58         GBOB         Offatts Bayou, Galveston Bay, TX                               .......................................... 210
                      59         GBSC         Ship Channel, Galveston Bay, TX                                .......................................... 219
                      19         SI.BB        Blue Buck Point, Sabine Lake, LA                               ......................................... 225
                      20         CLSJ         St. Johns Island, Calcasieu Lake, LA                           ....................................     231
                      60         CLLC         Lake Charles, Calcasieu Lake, LA                               ........................................ 237
                      21         JHJH         Joseph Harbor Bayou, J. Harbor Bayou, LA                                    ............................ 243
                      22         VBSP         Southwest Pass, Vermilion Bay, LA                              ......................................   249
                      23         ECSP         South Point, East Cote Blanche, LA                             .......................................  255
                      24         ABOB         Oyster Bayou, Atchafalaya Bay, LA                              .......................................  257
                      25         CLCL         Caillou Lake, Caillou Lake, LA                      .............................................       263
                      26         TBLB         Lake Barre, Terrebonne Bay, LA                                 ........................................... 269
                      Z7         TBLF         Lake Felicity, Terrebonne Bay, LA                              ......................................... 275
                      28         BBSD         Bayou St. Denis, Barataria Bay, LA                             .......................................  281
                      29         BBMB         Middle Bank, Barataria Bay, LA                                 ........................................... 287
                      61         BBTB         Turtle Bay, Barataria Bay, LA                       ..............................................      293
                      65         MRTP         Tiger Pass, Mississippi River, LA                              ......................................... 299
                      64         MRPL         Pass a Loutre, Mississippi River, LA                           ...................................   @. 305
                      30         BSBG         Bay Gardene, Breton Sound, LA                                  ..............................I .............. 312
                      31         BSSI         Sable Island, Breton Sound, LA                                 ............................................ 318



                                                                                         v









                   32       LBMP        Malheureux Point, Lake Borgne, LA                      ..................................... 325
                   62       LBNO        New Orleans, Lake Borgne, LA                           ............................................. 331
                   63       LPGO        Gulf Outlet, Lake Pontchartrain, LA                    ..................................... 337
                   33       MSPC        Pass Christian, Mississippi Sound, MS                      ..................................W
                   34       MSBB        Biloxi Bay, Mississippi Sound, MS                      ......................................... 350
                   35       MSPB        Pascagoula Bay, Mississippi Sound, MS                       ................................ ow
                   36       MBCP        Cedar Point Reef, Mobile Bay, AL                       .......................................... 362
                   66       MBHI        Hollingers Island Channel, Mobile Bay, AL                        ........................... 369
                   79       MBDR        Dog River, Mobile Bay, AL               .................................................... 375
                   67       PBPH        Public Harbor, Pensacola Bay, FL                       ......................................... 381
                   37       PBIB        Indian Bayou, Pensacola Bay, FL                        .......................................... 387
                   80       PBSP        Sabine Point, Pensacola Bay, FL                        ............................................ 393
                   73       CBJB        Joe's Bayou, Choctawahatchee Bay, FL                          .................................. 399
                   38       CBSR        Off Santa Rosa, Choctawahatchee Bay, FL                        ............................. 405
                   82       CBBL        Ben's Lake, Choctawahatchee Bay, FL                         ................................... 413
                   39       CBPP        Postil Point, Choctawahatchee Bay, FL                     .................................. 419
                   83       CBBB        Boggy Bayou, Choctawahatchee Bay, FL                        ................................ 425
                   74       PCLO        Little Oyster, Panama City, FL                         .............................................. 431
                   68       PCMP        Municipal Pier, Panama City, FL                        ......................................... 437
                   40       SAWB        Watson Bayou, St. Andrew Bay, FL                       ....................................... 443
                   41       APDB        Dry Bar, Apalachicola Bay, FL                          .............................................. 449
                   42       APCP        Cat Point Bar, Apalachicola Bay, FL                       ...................................... 4M
                   75       AESP        Spring Creek, Apalachee Bay, FL                        .......................................... 461
                   69       SRWP        West Pass, Suwannee River, FL                          ............................................ 467
                   43       C1KBP       Black Point, Cedar Key, FL               ................................................... 473
                   70       TBOT        Old Tampa Bay, Tampa Bay, FL                           ............................................ 481
                   44       TBPB        Papys Bayou, Tampa Bay, FL                             ................................................ 487
                   45       TBHB        Hillsborough Bay, Tampa Bay, FL                        ......................................... 493
                   46       TBCB        Cockroach Bay, Tampa Bay, FL                           ............................................ 499
                   47       TBMK        Mullet Key Bayou, Tampa Bay, FL                        ......................................... 505
                   77       TBKA        Knight Airport, Tampa Bay, FL                          ............................................ 511
                   76       TBNP        Navarez Park, Tampa Bay, FL                   .............................................. 517
                   48       CBBI        Bird Island, Charlotte Harbor, FL                      ......................................... 5M
                   71       CBFM        Fort Meyers, Charlotte Harbor, FL                      ........................................ 529
                   49       NBNB        Naples Bay, Naples Bay, FL                .................................................. 535
                   50       RBHC        Henderson Creek, Rookery Bay, FL                       ....................................... 541
                   51       EVFU        Faka Union Bay, Everglades, FL                         ........................................... 547
                   87       FBFO        Flamingo, Florida Bay, FL                ................................................... 553
                   88       FBJB        Joe Bay, Florida Bay, FL                  ....................................................... 559
                   81       BBEF        Bahia Honda Key, FL              ........................................................... 565
                   84       PRBB        Bahia de Boqueron, Puerto Rico, PR                     ...................................... 571
                   85       PRBM        Bahia Montalva, Puerto Rico, PR                        .......................................... 577
                   86       PRBJ        Bahia de Jobos, Puerto Rico, PR                        ............................................ 583








                                                                              vi










                                     FUM SAMPLING AND LOGISrflCS


                     This document provides a detailed description of the sampling sites in the
               Gulf of Mexico and Caribbean for the NOAA National Status and Trends (NS&T)
               Mussel Watch Program for Year IEK (Period 2, Phase 4). The report contains
               detailed maps and site descriptions for all sites that have been sampled during the
               first nine years of the program. Locations (Nominal Site Centers) provided are
               the most recent available for a specific site and are based on chart data and Global
               Positioning System (GPS) positions, when available. GPS positions have been
               obtained for approximately 75% of the sites sampled. Loran C positions are not
               available for the Caribbean, therefore only GPS positions are provided. GPS and
               Loran C data are provided in Appendix A.


               SampUng History


                     In 1986, GERG undertook the first NS&T Mussel Watch site sampling in
               the Gulf of Mexico. GERG's efficiency in field sampling is attested to by the fact
               that during the course of the past eight years of the project, the Gulf Coast field
               collection effort increased from an initial 50 sites in Year 1 to 88 sites by Year EK.
               The days in the field which were required to complete the sampling decreased
               from 48 days in Year I to 26 days in Year IV, despite the 40% increase in
               sampling effort. Years II and III sampling showed similar efficiencies,
               requiring 31 and 30 days, respectively. The sampling dates established in Year V
               dictate a time frame during which all subsequent sampling will be completed:
               Texas and western Louisiana sampling is done in December, and sites in eastern
               Louisiana, Alabama, Mississippi, and Florida are sampled in January. In Year
               VII we added Puerto Rico, which is sampled in February. No new sites were
               added in Year VIII. Two new sites were added in Florida in Year IEK. A total of
               32 days were spent in the field, sampling the 51 Year IEK sites, eleven of which
               were travel days.
                     The sites from which we have collected oysters in the Gulf of Mexico have
               not been limited to the coastal embayments, but have included a number of sites
               which face the open Gulf and can only be reached by long, boat transits.
               Stretching from Port Isabel in the lower Laguna Madre of Texas to Bahia Honda
               Key in Florida, the study area encompasses over 1500 straight line' coastal miles.
               The actual distance to be traversed is appreciably longer since access to many


                                                         1









             remote areas within the embayments is by circuitous route. A single field crew
             sampling half of the Gulf sites would travel over 8000 miles during a field season.
             This limited access to the shoreline combined with the very shallow waters of
             most of the bays (63% of all maintenance dredging in the continental U.S. is done
             in the Gulf of Mexico) has supported our original contention that the Gulf of
             Mexico sites are the most difficult U.S. coastal sites to sample.


             Bivalve Disbibutional Patterns in the Gulf of Mexico


                    Distributional patterns of molluscan assemblages are dependent on water
             depth, substrate type, turbidity, salinity, and wave energy. Parker (1960) defined
             molluscan assemblages along the Gulf coast based on salinity and substrate type.
             The important inshore, estuarine, and lagunal assemblages are as follows:


             I .    River Influenced, Low-Salinity Assemblage - The largest and most
                    abundant bivalves in this assemblage are:


                       Rangia cuneata - Common in beds all along the Gulf coast, large;
                       Rangia flexuosa - Less abundant than R. cuneata;
                       Polymesoda carolinensis - Missing from Mobile, Alabama, east and
                          Matagorda Bay, west and south and
                       Macoma mitchelli - common in most low salinity lagoons and estuaries;
                          small.


                    This assemblage is characteristic of soft-bottom areas surrounding river
             mouths, where the salinity is usually less than 10%o. Typically, the numerically
             dominant mollusk in this zone is Rangia cuneata. Rangia cuneata, flexuosa, and
             Polymesado carolinensis have life spans of several years whereas Macoma
             mitchelli is an annual species. Of the longer-lived species, Rangia cuneata is also
             the biomass dominant in this assemblage.


             IL     L)elta - Front Distributa1y and Interdisciplinary Assemblage - The largest
                    and most dominant bivalves in these assemblages are:


                       Rangia cuneata - not as abundant as in river influence as'semblage;
                       Rangia flexuosa - more, abundant in interdistributary bays;


                                                      2










                        Macoma mitchelli - rare;
                        Crassostrea       virginica      very abundant in higher salinity
                           interdistributary bays; and
                        Petricolar pholadiformis - common, but small.


              III. Low Salinity Oyster Assemblage - The largest and most dominant bivalves
                     in this assemblage are:


                        Crassostrea virginica - predominates in this zone; and
                        Brachidontes recurvus - at times very abundant.


                     Oyster reefs are abundant where salinities range from 10-30,7oo. Most
              oyster reefs are found in relatively shallow waters. Typically, large reefal
              structures are formed at right angles to the dominant current flow. These reefs
              are easily located and are a major geologic feature in most of the bays along the
              Gulf Coast that were included in this study. Crassostrea virginica is the biomass
              dominant as well as the most numerically-abundant large bivalve in the
              assemblage. The mytilid Brachidontes recurvus is usually found associated with
              oyster reefs. However, it is not as abundant nor as large, and it has no previous
              baseline established in the Mussel Watch programs. Furthermore, it rarely
              occurs in other assemblages where C. virginica is an important component.


              IV. High Salinity Oyster Assemblae - The largest and most abundant bivalves
                     in this assemblage are:


                        Anomia simplex - common, but small;
                        Brachidontes exustus - common, attached to oyster valves; small;
                        Diplothyra smithii - rare; and
                        Ostrea equestris - abundant, long lived species.


                     High salinity reefs form near inlets where relatively high salinity water (34-
              36%) is constantly renewed by tides. The substrate is old shell. Ostrea equestris is
              generally abundant and replaces C. virginica when salinities rise above 300loo.
              Over the long-term, drought cycles frequently result in the replacement of C.
              virginica with 0. equestris (and vice versa in wet years) along most of the Gulf
              coast (Parker, 1960). Thus, this assemblage is effectively identical to the Low


                                                        3









              Salinity Oyster Assemblage except where salinity causes the replacement of
              species with other closely related species. In this assemblage, 0. equestris
              typically is the biomass dominant.


              V.     Hypersaline Lagoon Assembla - The largest and most abundant bivalves
                     in this assemblage are:


                        Mulinia lateralis - abundant but small;
                        Tellina tampaensis - moderately abundant; and
                        Anomalocardia auberiana - very abundant


                    Hypersaline lagoons and estuaries are common along the lower Texas coast
              and have salinities ranging from 40-8096. The species in this assemblage are
              very abundant but are short-lived and small. The numerical dominant in this
              zone is Anomalocardia auberiana.
                    In the previous EPA Mussel Watch Program, genera from the family
              Mytilidae (the mussels) were used as the organisms of choice for portions of the
              geographic range of the mussel watch program. Along the Gulf coast, however,
              mussels that do occur (i.e., the genera Brachidontes, Lioberus, Lithophaga,
              Modiolus, Geukensia, and others) are often too small and frequently not abundant
              or widespread enough to be considered as target organisms. Previous baseline
              organisms along the Gulf Coast for the EPA Mussel Watch Program were all
              species in the family Ostreidae (viz. Crassostrea virginica and Ostrea equestris).
              These two species are frequently biomass dominants, abundant and easily
              resampled from their respective habitats.
                    The American oyster, C. virginica, occurs in intertidal and sub-tidal
              habitats all along the Gulf coast (Scott and Lawrence, 1982). C. virginica is
              sufficiently widespread so that it has been able to be collected at the majority of the
              designated sites.      C. virginica typically inhabits coastal and lagunal
              environments with salinity levels normally varying between 20-30%o, although
              populations at both lower and high salinities are known. Moreover, as shown
              above, it is one single species common to nearly all the Gulf Coast and is usually
              biomass dominant where it occurs. The oyster reefs produced by C. virginica,
              which make up the bulk of the sites selected for this portion of the NOAA Mussel
              Watch Program, are permanent and for the most part large features which are
              easily located for sampling and resampling. In addition, living oysters usually


                                                         4









               are abundant on these features and are recruited yearly to the reefs; thus, these
               areas will be able to sustain repeated sampling over a ten year plus period with
               minimum impact on the population structure of the reef. Clearly, due to the
               previous baseline established for this species, its abundance, large size, and
               ubiquitous occurrence along the Gulf coast, C. virginica is the target organism of
               choice.
                    Although C. virginica has a particularly wide salinity tolerance, a few areas
               that have been designated as sites are outside its range. In areas of low salinity
               which do not support C. virginica, Rangia cuneata is the dominant bivalve. R.
               cuneata is relatively large and abundant. It is the characteristic organism of the
               low salinity assemblage and it occurs in most low salinity habitats along the Gulf
               Coast. R. cuneata is usually found in abundance at the sediment-water interface
               on soft bottoms.
                    We have attempted to find C. virginica in the high salinity bays in the lower
               Laguna Madre between Corpus Christi and Port Isabel with limited success. 0.
               equestris, an ecological equivalent, usually replaces C. virginica in these areas.
               0. equestris is usually associated with hard bottoms or mangrove roots and is
               abundant and large. It has an established baseline for comparison because it
               previously was used in the EPA Mussel Watch program. In addition, it belongs to
               the same mollusk family (Oestreidae) as C. virginica. In most habitats which
               usually do not support C. virginica, 0. equestris can be collected and is the
               organism of choice.
                    In hypersaline lagoons (i.e., Baffin Bay, Texas) where C. virginica and 0.
               equestris are absent, Anomalocarida auberiana could be sampled. This species is
               small, yet it is very abundant, reaching population levels of -2000 per M2 in some
               instances.
                    Because of the concerns about species effects, none of the alternate species at
               the sites which do not support C. virginica have been collected to date.


               Field loewdcs


                     Collection and field processing of oyster and sediment samples was
               conducted by GERG utilizing small boats and a portable self-contained laboratory
               (used in Years I - VI) which was specifically equipped for sample_ processing and
               storage. A field collection crew consisted of two people (sometimes three,
               depending on the   site) using a trailered outboard boat. Table 1 summarizes


                                                        5









              collection methods and site accessibility. In Years VII through IX, the
              processing was not conducted in the mobile lab. Samples were collected, packed
              on blue ice, and shipped back to our labs in College Station by Federal Express for
              processing the next morning. Samples from Puerto Rico were shipped via
              American Airlines as perishable items and were received at the GERG laboratory
              in less than 48 hours.
                    The Gulf of Mexico field collections were made in four sampling legs. The
              first leg departed College Station, sampling the -12 sites within a 200 mile radius
              of College Station (Brazos River, Galveston Bay, and as far east as western
              Louisiana) in a 5-day period. Samples were returned to College Station by Federal
              Express, private aircraft, and/or the returning field crews. The next sampling
              leg was based at Port Lavaca. During this five day period, two boat crews
              completed sampling the -19 remaining Texas sites and returned to College
              Station. Following the Christmas holidays, sampling resumed with two boat
              crews who sampled from central Louisiana, through Mississippi, to Alabama.
              Upon completion of the - 20 sites as far east as Mobile, Alabama, the sample
              crews returned to College Station. The last leg sampled 24 sites in Florida. After
              completion of the Gulf sampling, the boat crews returned to College Station. In
              Year VII a fifth sampling leg was added in February to sample Puerto Rico,
              where local fishermen were employed to use their boats to take us to collection
              sites.


              LqOsfic: Com4derations


                    Major logistical concerns in sampling the Gulf of Mexico oyster sites
              primarily involved access to the variety of sampling sites (and collection gear) and
              to a lesser degree permitting/politics. In the Caribbean, logistical concerns were
              much more complex since all collection gear had to be carried aboard airplanes
              and permitting and regional politics played a much larger role.
                       In the Gulf of Mexico, one major problem concerned accessibility to the
               oyster sites. Some samples were little trouble to obtain, such as from rock rubble
              beneath a highway bridge in Galveston,   but some were difficult, such as ones that
               required a 35 mile drive from the nearest motel to the launch, a 15 mile boat ride
               through Louisiana marshes, and 10 miles alongshore in the open, Gulf to a site
               that had to be sampled by dredging. Not only was this situation difficult', but the
              site was situated within a private lease which extended to all oyster bottoms in the


                                                        6









                             Table 1. Collection Methods and Site Accessibility.


                        Site   Designator    Collection Method     Access to Site



                         I        LMSB               H                  S.B
                         52       LMPI               H                   A
                         78       LMAC               H                  B.S
                         2        CCNB               H                  S.B
                         3        CCIC               H                   B
                         53       CCBH               H                   A
                         4        ABLR              T/H                 L.B.
                         54       ABHI               H                  A.B
                         5        CBCR               H                   B
                         6        MBAR               H                  L.B
                         7        SAPP               D                 L.B.0
                         8        SAMP               H                   B
                         9        ESSP               H                   B
                         10       ESBD              T/H                  B
                         11       MBLR              H/D                  B
                         12       MBGP               D                  B.0
                         13       MBTP               H                 L.B.0
                         14       MBEM               H                   B
                         56       M13CB              H                  B.S
                         55       MBDI               H                  B.S
                         72       BRCL               H                  B.S
                         67       BRFS               H                   A
                         15       GBYC              T/D                  B
                         16       GBTD             D/H/T                B.0
                         17       GBHR              D/T                L.B.0
                         18       GBCR               H                   B
                         58       GBOB               H                   A
                         59       GBSC               T                  S.B
                         19       SLBB              H/D                  B
                         20       CLSJ               T                   B
                         60       CLLC               H                   B
                         21       JHJH               H                   B
                         22       VBSP               D                 L.B.0
                         23       ECSP             NONE                L.B.0
                         24       ABOB              D/H                L.B.0
                         25       CLCL               D                  L.B.





                                                     7










                                        Table 1. Continued


                      Site  Designator   Collection Method   Access to Site



                      1%       TBLB              H                 B
                      27       TBIY              H               L.B.0
                      28       BBSD             'H/D              B.0
                      29       BBMB              H                 B
                      61       BBTB             D/T              B/L.0
                      65       MRTP              H                BIL
                      64       MRPL             T/D              B/L.0
                      31       BSSI              D               L.B.0
                      30       BSBG              H                B.0
                      32       LBMP              H                 B
                      62       IBNO              H                B/L
                      63       LPGO              D                B/L
                      33       MSPC             RIT               A.B
                      34       MSBB              H                A.B
                      35-      MSPB             T/D                B
                      36       MBCP              T                 B
                      66       MBHI              T                 B
                      79       MBDR              T                B.S
                      67       PBPH              H                 B
                      37       PBEB           H/T/D                B
                      80       PBSP              H                B.S
                      73       CBJB              H                 A
                      38       CBSR           H/T/D                B
                      82       CBBL              H                 B
                      39       CBPP*             H                 A
                      83       CBBB              H                 B
                      74       PCLO              H                 B
                      68       PCMP              H                 B
                      40       SAWB              H                 B
                      41       APDB              T               L.B.0
                      42       APCP              T                 B
                      75       AESP              H                 B
                      69       SRWP             H/T                B
                      43       CKBP              H               L.B.0
                      70       TBOT              H                 A
                      44       TBPB              H               AorB".





                                                 8











                                  Table 1. Continued


                  Site  Designator Collection Method Access to Site



                   45     TBBB           H              B
                   46     TBCB           H              B
                   47     TBMK           H              A
                   77     TBKA           H              A
                   76     TBNP           H              A
                   48     CBBI           H             L.B.0
                   71     CBFM           H              A
                   49     NBNB          H/T             B
                   50     RBHC           H              B
                   97     FBFO           H              B.S
                   88     FBJB           H             B.L.0
                   51     EVFU           H              B
                   81     BBEF           H              A
                   84     PRBB           H              B
                   85     PRBM           H              A
                   86     PRBJ           H              B.L


                  name changed from CBSP to CBPP.
                 H  hand sampling; T = tong sampling; Ddredge sampling
                 B  boat required; S = time to reach site < I hr; L = time to reach site >1 hr;
                 0  site located in large open water or required to cross; Aaccessible by
                 auto by wading from shore.




















                                         9









             bay system. R was owned by a family who initially met us at the boat launch and
             who were adamantly against our sampling from their lease. The site was located
             on what may be the most prolific oyster beds in Louisiana (and, therefore, on the
             entire Gulf coast), and was exposed to outflow from the entire Atchafalaya
             drainage basin.
                   Particularly in the Gulf, we had need of a relatively large, fast and
             seaworthy boat that had a draft shallow enough to traverse the shallows en route
             to the site and to get on the reef for sampling.
                   Proper equipment and navigational gear was needed to safely cross the
             Mississippi Delta area, where landforms and bayous in and around the delta
             showed essentially no conformity to the most recent NOAA navigational charts,
             as was verified by our direct experience in the field and from very recent aerial
             photographs.
                   The other sampling extremes were typified by sites such as Cockroach Bay,
             which was accessible only with a very shallow draft boat and which required a
             two mile ride through an uncharted mangrove swamp and across oyster bars,
             over which the boat had to be dragged even on a flood tide. On the other hand,
             sites such as Dry Bar in Apalachicola (where dredges were absolutely forbidden)
             required about a 7 mile ran across the open bay in the Boston whaler to a site so
             shallow that it was collected by hand while wading.
                   The distance between the Gulf sites measured 1500 nautical miles when the
             straight line distances between nearest sites were totalled. However, a single boat
             crew completing half the sites over the range from Laguna Madre to Naples drove
             over 8000 miles in a sampling season because of the limited and circuitous routes
             to launching ramps and sites.
                   In the Caribbean, transportation on land and on the water had to be
             arranged on each island and at each collection site. There were few commercial
                                                        ore, local fishermen had to be located at
             or recreational boat rental sources; theref
             each collection site for which boats were needed. Local fishermen were
             sometimes hesitant to take scientists to their oyster harvesting areas since they
             believed that there could be some interference with their livelihood.


             Permits


                   Collection of oysters in all the affected states was regulated by state
             agencies. A scientific collecting permit was required prior to collection in Texas,


                                                      10









               Louisiana, Alabama, Florida and the Virgin Islands. Prior written permission
               was required in Mississippi. Collections in Puerto Rico were made under the
               University of Puerto Rico permit. Additionally, the preponderance of privately
               leased oyster beds in Louisiana required that we obtain permission from the
               individual lease holders for those instances where the site was (or was thought to
               be) located in a leased area.
                      Permit applications were submitted to and obtained from the following:

                      Texas -        Mr. Steve Schwelling
                                     Permits Branch, Resource Protection Division
                                     Texas Parks and Wildlife Department
                                     4200 Smith School Rd.
                                     Austin, Texas 78744

                      Louisiana -    Ms. Karen Foote
                                     Louisiana Department of Wildlife and Fisheries
                                     P.O. Box 98000
                                     Baton Rouge, LA 70898

                      Mississippi -  Mr. Joe I. Gill, Jr.
                                     Miss. Dept. of Wildlife, Fisheries and Parks
                                     Deputy Director, Coastal Operations
                                     2620 Beach Blvd.
                                     Biloxi, Ms 39531

                      Alabama -      Mr. Charles Grimsley
                                     Game and Fish Division
                                     Department of Conservation and Natural Resources
                                     64 North Union Street
                                     Montgomery, AL 36130

                      Florida -      Ms. Kat Ethridge
                                     Florida Dept. of Natural Resources
                                     3900 Commonwealth Blvd., Room 813
                                     Tallahassee, Florida 32303

                      Florida -      Everglades National Park
                                     Dan Foxon
                                     Everglades national Park
                                     P.O. Box 279
                                     Homestead, F1 33030

                      Virgin Islands - Dr. Lawrence Benjamin
                                     Assistant Commissioner
                                     Department of Planning and Natural Resources
                                     45 Estate Nisky
                                     Charlotte Amalie, St. Thomas, V.I., 00802












                     Puerto Rico - Mr. Javier Velez Arocho
                                    University of Puerto Rico
                                    Sea Grant Program
                                    P.O. Box 5000 RUM-UPR
                                    Mayaguez, Puerto Rico 00681-5000

                       g Gear


                     Boats


                     Two boats were used during the course of collection. A 17' Boston Whaler
              with an 88 hp engine and electric tilt/trim and a 16' Whaler with an 88 hp engine
              provided the greatest versatility and utility in sampling. Each boat was equipped
              with dual fuel tanks, a VHF radio, Raytheon Loran C, a fathometer, and
              emergency/safety equipment. A handheld GPS receiver was used on one of the
              boats during Year VII and later years. The boats were trailered to a launch site
              near the sampling site. Due to the remote location of several sites, running times
              of up to 3 hours were required to get from the launch site to sampling sites.


                     Radio Coinmunications


                     All vessels were equipped with CB/VHF marine radios. This allowed us to
              coordinate sampling activities between both boats when they worked
              simultaneously and kept us in contact with the Coast Guard and other vessels
              operating in the area if emergencies or difficulties arose. It also kept us abreast of
              coastal weather conditions which were broadcasted continuously on the VHF
              weather frequencies.


                     Safety Considerations

                     All boats were equipped with Coast Guard-required safety and emergency
              equipment (life jackets, lights, reserve fuel, first aid kit, etc.). Our group conducts
              extensive field activities, approximately 120 man months on oceanographic
              cruises per year, including over 40 weeks on chartered boats that we fully equip
              and rig out; thus, we were well aware of applicable safety considerations and the
              consequences of failure to prepare for these contingencies on a fie'ld such'as the
              NS&T program.



                                                        12









              Sample Collection


                     Oyster Collections

                     Oysters were collected by hand, with tongs, or by using a dredge (Table 1).
              Collection by hand was the method of choice and was used at intertidal and
              shallow subtidal sites. Loose oysters were simply picked from the reef and were
              separated from attached shell debris or other oysters by using an oyster knife or
              chipping hammer. In some areas where the use of a dredge was prohibited (e.g.
              Apalachicola Bay), subtidal sites were sampled using oyster tongs. The oysters
              were separated from one another by using oyster knives or chipping hammers.
              At the deeper subtidal sites and where oysters were obtained directly from
              commercial oyster fishermen at their privately leased sites, the oysters were
              collected with steel or stainless steel oyster dredges. Clumps of oysters and shell
              were separated to individual oysters as before.


                     Sediment Collections


                     Wh ere water depth permitted, sediments were sampled by hand using a
              Teflon-coated scoop. Prior to each use the scoop was cleaned and rinsed with
              acetone and then methylene chloride to remove any trace of residual organics.
              Spent solvent was collected and returned to GERG for proper disposal. The upper
              1 cm of sediment was removed by the scoop and was placed into the appropriate
              container. In water depths where direct sampling was not possible, a stainless
              steel box corer mounted on a long pole or on a rope with a messenger was used to
              bring a sample of the substrate on board the boat and the Teflon-coated scoop was
              used to remove the top 1 cm subsamples for analysis.


              Field Processing


                     Salinity and Temperature


                     At every site, the temperature of the surface water was recorded. Salinity
              was measured either at the site with a temperature compensating refractometer
              or a sample was collected and returned to the mobile field lab-oratory where




                                                      13









              salinities from a number of sites were measured at constant temperature with a
              calibrated refractometer.


                    Oyster PivcessfiV


                    Oysters were processed in GERG's sample processing laboratory. Rapid
              processing of oysters was required because of the coincident collection of
              histopathological. data after collection and prior to freezing. In addition, the
              histopathological procedure required follow-up handling and immediate cultures
              which could be achieved only under laboratory conditions. We used this
              opportunity not only to provide the histopathological. data but to provide additional
              data on relevant bivalve parameters. This process allowed the field collections to
              proceed efficiently while separating the collection activities from the processing
              functions.
                    As samples were collected on the boat, they were segregated and labeled
              according to station and replicated. Efforts were made to retain organisms in the
              same size range for sampling so that organisms pooled for analysis at a site and
              the replicates were of similar age or maturity. Samples were stored in ice chests
              aboard the boat until the day's sampling was complete. At that time they were
              transferred to Freez-Safe insulated containers which were packed with frozen
              blue ice for shipping. The samples were driven to the nearest Federal Express
              office for next-day delivery to GERG. The processing protocols for oysters are
              shown in Figure 1.
                    Cleaning and Shucking - Oysters were scrubbed free of mud and debris
              while at the collection site. Pure bristle brushes and water from the collecting site
              were used for cleaning. Oysters destined for organic and histopathological
              analyses were measured for length and displacement volume and were opened
              and processed in the GERG laboratory.
                    Dissecting and Gonadal Preservation - Twenty oysters from each of three
              sampling stations at every site were processed for histopathological and gonadal
              development study. After the initial displacement volume of the washed oysters
              was determined, they were arranged on a stainless steel tray. Each oyster was
              measured (axial length) and was opened with a solvent-washed stainless steel
              oyster knife. A complete cross section of gonadal tissue was exti      sed froija each
              oyster and was placed into an individual tube of Bouins solution. A small snip of
              mantle tissue was also removed and was placed into individual tubes of fluid


                                                        14






                    Sample Processing Protocol
                                      Oyster Sites



                              Measure First
                               Displacement
                               Volume (VI)


                                 Open Shell


                                Measure
                                Interaxial
                                 Length
                                    I
                               Snip Mantle
                                 Section
                                    I
                                 Cut Off
                               Bottom Half
                                    I                                      Culture
                               5 mirn Cross                               tube for
                                                                          Perkinsus
                                 Section:                                1 snip/tube
                              First 10 Oysters                            20 tubes

             Bonin's           Cut Bottom
             Solution           Adductor
             In Vial            Remaining
                                 Tissue                                  LabelTube
                I                   I                                      & Cap
              Label          Jar For Organics             Measure            I
             & Store                                       Second          Store
            Ambient                                     Displacement     Ambient
                              Label Outside             Volume (V2)
                             Jar, Teflon Lmier               I
                                                       F
                                                          Discard
                                                           Shell








                         Figure 1. Sample processing protocol for oyster sites.


                                               15




                        -- --------







              thiglycolate medium (FTM) for the determination of the incidence of the oyster
              parasite Perkinsus marinums.
                    Trace Organic Processing - The remaining oyster tissue was excised from
              the shell and was placed into a pint mason jar which had been combusted in a
              muffle furnace to completely remove trace organics. The. tare and gross weight of
              the jar and contents (20 oysters) were recorded on the lab form with the individual
              lengths of the oysters. A Teflon liner was placed under the jar seal, and the
              labeled sample jar (one for each of three stations in a site) were frozen in
              laboratory deep freezers.
                    Trace Metal Processing - A separate 20 oysters from each of three stations
              within a site were washed as above and were set aside in plastic trays for metal
              analyses. The oysters were collectively measured for the initial displacement
              volume. Because of their sharp edges and the need to ensure that they remained
              closed prior to processing, the oysters had to be individually wrapped in singlefold
              paper towel and bound with rubber bands before they were double bagged in
              plastic trash bags, labeled and stored in the freezers. All opening and further
              processing of oysters (measurement of shell length and second displacement
              volume) was done at the analytical laboratories under clean room conditions.
                    National Biomonitoring Specimen Bank (NBSB) - Bivalves from five sites on
              each coast were sent to the NBSB, The NBSB archives were housed at the
              National Institute of Standards and Technology (NIST, formerly National Bureau
              of Standards). Processing of these samples was conducted in the GERG facilities.
              The processing protocol is depicted in Figure 2. A and B replicates were
              comprised of 10 bivalves each from the three stations at a site (30 individual
              specimens in A and B).
                    The composite sample of 10 oysters from each station was scrubbed clean of
              mud and debris by using the pure bristle brush. The bivalves were placed into a
              teflon bag and care was taken to keep the inside of the bag at the point of sealing
              dry. Air was evacuated from the bag as much as was practical, and the bag
              opening was sealed with the heat sealer. Air-tightness of the first seal was
              checked by depressing the bag and listening for escaping air. The bag was
              resealed as necessary and a second seal was then made about 1/2" out from the
              first.
                    The sealed bag was then placed into another teflon bag With the sample
              label between the two bags. The sealing process was repeated on      the outer bag,
              and the resultant double-bagged sample was placed on dry ice for shipment to


                                                       16






                       Sample Processing Protocol
                                       NIST Bivalve Sites
                                      (10 Sites only per Year per Coast)


                                                           3 Stations Per Site
                                                                Is or Oysters
                                                           Musse








                                                  20 Bivalves
                                                from Each Site





                                                  Teflo Bag






                                                  Double Bag




                                                  [FREEZIE





                                                  Store On
                                                   Dry Ice
                                                    Labe































                             Figure 2. Sample processing protocol for mussel sites.


                                                     17









              NBSB. A data form provided by NBSB was completed for each site and sent along
              with the samples.
                    Shell Preservation - Oyster shells that were processed and opened in the
              laboratory (the sets of 20 for histo, gonadal, and organic analyses) were. retained
              and archived for possible future study. This set from each site already had the
              most complete data set (length, wet weight, mantle cavity volume, as well as
              contaminant, histo, and gonadal index) and would be of greater value than a set
              taken separately or for only one parameter. The shell pairs were placed into cloth
              bags (soil sample bags), labeled, and stored in 5 gallon plastic buckets with
              ethanol as a preservative.


                    Sediment Proeessing


                    Sediment processing was essentially completed at the site, whether on the
              boat or on the shore in the case of shore-accessed sites. The processing protocols
              for sediment samples, including those for NBSB archival, are shown in Figure 3.
                    Trace Organics and Trace Metals - The separate samples for organics and
              trace metals, contained in a pre-combusted half pint glass jar and a heavy duty
              pint zip-lock bag, respectively, were labeled and assigned a unique identifier code.
              They were shipped to GERG and stored in the freezers until analyzed.
                    Grain Size - Sediments collected for grain size analysis were stored in zip-
              lock or whirl-pack bags at ambient (above freezing) temperatures. As with the
              previous samples they were labeled with collection and site information and given
              a discrete identifier code.
                    Sediment Ancillary Measurement - Sediment ancillary measurements
              were run on aliquots of the samples taken for organic analyses; thus, no
              additional sampling or processing was required in the field.
                    National Biomonitoring Specimen Bank - At five sites per coast, sediments
              from all three sites were composited into two replicates (A and B) for NBSB
              archival. The procedure is illustrated in Figure 3.
                    Subsamples from all three stations at a site were combined into a
              composited sample. The subsamples were placed in teflon jars. The jars were
              filled only to within V2" to 1/4" of the top to allow for expansion during freezing.
              The label was taped to the outside of the jar, and the sediment samples were
              stored on dry ice for air shipment to NBSB. The NBSB data form'Was completed
              and sent with the samples.


                                                       18






                              Sample Processing Protocols
                                                              Sediments







                                                                  ......      3 Box Cores
                                                                              Per Station






                      NIST Sample                          Remove Upper 1 cm
                      10 Sites Only                           Trace     metals          Oraanics            Grai-)
                               3 Scoops                                                                         n        Size
                                  per                         Center Scoop            Inner S                 utside Scoop
                                                                  35 gm                      9 coop 1      10 70 gm
                               Box Core                                                   70 M
                               20 g Each
                                     I                            I Subsample             1 Subsample          1 Subsample
                            From 3 Stations                       From Each              From Each             From Each
                                 Per ISite                        Box Core               Box Core              Box Core
                              Teflon Bag                                                                      I
                                                              1 Ziplock Bag             1 Jar Per           1 Ziplock Bag
                                                               Per Station               Station             Per Station
                                 Label                        100 gm Total               200 gm
                             Double Bag                              I                       I
                                     I                        Label Outsidj          Label Outside          Label Outside
                          F FERin                              Double Bag              Teflon Lid            Double Bag
                                     I                                                    Liner
                               Store On                                                      I
                              Liquid N2                                                                      AMB
                                                         ;
                                                                              3
                                                                              P
                                                                              0

                                                                              e
                                                                              ox
                                                                              r St
                                                                                 C
                                                                                 at!,










                                                                              tis
                                                              Trae     me a7
                                                                  c
                                                              Center Scoop
                                                                  35 gm
















                                                                                      EZE                            IENT



                                     big@ sm'' p@








                                     Figure 3.      Sample processing protocol for sediment sites.


                                                                         19









                    Sample Handling and Chain-ofCustody


                    Sample collection was under the supervision of the field party chief who
             recorded the data and checked off the samples that were acquired against a
             prepared station list. Samples and copies of the data sheets were shipped to the
             laboratory manager at GERG. The lab manager supervised the processing of
             samples in the lab, the labelling, and the storing. The manager also prepared an
             inventory list of samples that were collected and were to be transferred. All
             samples and records were transferred to the Quality Control Manager who
             checked the samples against the prepared station list and stored the samples for
             subsequent disbursement to the different analytical groups and final storage.
                    NBSB samples were shipped directly with sample documentation and
             transfer forms that were provided by NIST. A critical part of this transfer process
             was arranging shipment so that the samples did not arrive on a weekend             and
             notifying the NBSB contact by telephone of the expected arrival time.


             Field Sampling CAteria


                               of Stations and Sites


                    A site, the smallest geographic unit sampled, was selected to be
             representative of a target area, general location, or bay system. Once selected, the
             site was sampled at the same location each year. Within each site, three
             independent stations were sampled in order to characterize the site. Offshore
             subtidal sites were -400 m radius circles. Latitude and longitude of the site was
             measured at the center of the circle. Shoreline intertidal sites were defined as 100
             m linear areas along the tidal horizon to either side of the site center. Sediment
             sites were generally coincident with the bivalve sites. However, when subtidal
             bivalve sites did not meet the sampling criteria, separate sediment sites were
             selected. If at all possible, these sites were located within 2 kni of and exposed to
             the same water mass as the bivalve site. The sequence of events in selection of
             new sites is shown in Figure 4.
                    Eighty-five (85) sites were established in the Gulf of Mexico and three (3)
             sites in the Caribbean during the first nine (9) years of the NS&T program. For
             the first six years of the study three stations at each site were sampled and each
             station was characterized by three replicate composited samples taken for


                                                       20












                                                Bivalve sampling sl@te


                                                                                  Contact the COTR,
                        Sediment
                                                                                 then se
                                         YES                                             lect alternate
                     sampling from                                         NO
                                                                                 bivalve sampling site
                   within bivalve site
                                                                                   or don't perform
                                                                                      collection




                                         YES






                             NO


                 Select afterriate sediment                  Obtain                 Go to
                sampling site within 400m                   sediment          0, next station
                   radius of bivalve site                   samples                or site

                             NO



                                   t"U.  YES





                             NO


                  Collect best available
                  sediment within 2km
                  radius of bivalve site










                                   Figure 4.   Decision criteria for sediment sites.

                                                           21









              sediment analysis and a composite bivalve sample made up of 20 oysters.
              Selection of the three sampling stations was based on the historical locations as
              described for each individual site in this report. In Year VII the number of
              oysters collected at each of the previously sampled stations was reduced to 20 from
              22 oysters. The composite bivalve sample was made up of 7 to 8 oysters from each
              of the three stations.


                    Bivalve Site Selection Criteria


                    BivalVe sites were initially established according to the following criteria:

                 ï¿½  The site shall have indigenous oysters of a suitable size (7-10 cm for        C.
                    virginica or C. rhizophore) which are available for collection annually.

                 ï¿½  The site shall integrate contaminant accumulation from nearby                 or
                    surrounding areas and shall be outside the zone of initial dilution of a
                    dumpsite or point-source discharge.

                 ï¿½  The site should have sufficient bivalves, such that repeated annual
                    harvesting of 250-400 bivalves will not seriously deplete the resource.

                 ï¿½  The site should be suitable for follow-up sampling (e.g., not anticipated to be
                    be physically disrupted by development activities or dredging).

                 ï¿½  Sampling substrates will be limited to rock (including rip-rap), and or mud
                    (or mangrove roots in the case of C. rhizophorae). Artificial structure such
                    as pilings, and navigation aids will be avoided if possible in order to avoid
                    potential contamination.

                 ï¿½  Provided other criteria are met and where feasible, the bivalve sites should
                    coincide with historical monitoring sites (e.g., USEPA Mussel Watch
                    monitoring sites).
                    Each year the field sampling effort has reoccupied as many of the historical
              sites as possible. It has become necessary to relocate or abandon a few of the
              established oyster sites over the years due to one or more of the following
              circumstances:

                 ï¿½ Bivalve populations were no longer present.

                 ï¿½ A construction project or dredging activity precluded sampling.

                 ï¿½ Collection of bivalves was logistically impossible or would endanger the
                    field personnel.




                                                       22









                     Permission to sample a site or gain access to a site was denied by a
                     landowner or a leaseholder.
              When another location was chosen for sampling, a determination was'        made by
              the COTR as to whether the new location was considered a minor "relocation" of
              the site or whether the new site was significantly different and should have been
              considered a newly established site. Newly established sites were assigned a new
              site name and unique site codes. Sites minimally relocated could retain the same
              name or could be renamed if the new name better signified the relocated site.


                     Sediment Site Selection Critexia


                     In most cases sediment sampling coincides closely to the bivalve stations.
              Because of the affinity of the target species for and the ubiquitous distribution of
              depositional zones in Gulf of Mexico estuaries, coincident sediment and bivalve
              sampling was generally not difficult. The specific criteria for sediment sites

              were:


                  ï¿½  The site shall be subtidal (never exposed at lowest tides),

                  ï¿½  The site shall be exposed to the same water mass as the corresponding
                     bivalve site,

                  ï¿½  The site should be located as near as possible to and preferably not more
                     than 2 km from the bivalve site,

                  ï¿½  The site shall integrate contaminants from multiple sources in the
                     surrounding area but shall not reflect inputs from an individual point
                     source of contamination.

                  ï¿½  Previously sampled or historical Mussel Watch locations (e.g., EPA sites)
                     should be included/reoccupied where possible.


                     Field personnel were responsible for determining the suitability of the
              sediment sample. Only sites which were minimally (40 percent) fine sediment
              (silt and clay) were sampled for sediments. Samples that did not meet this grain-
              size criteria were not analyzed for the trace elements and trace organics.
              Therefore, grain size determinations were made prior to the chemical analysis.






                                                       23









                        Summary


                    Sampling Problems


                    It has been our intent to sample the locations prescribed by NOAA during
              Years I through IX of the program. However, in the field we have found that at a
              few sites, many factors worked against this both in the initial first year sampling
              and then again in the subsequent years when we returned to resample the same
              sites or new sites. Some of the prescribed locations could not be used because
              when we examined them in the field, they did not meet the most basic site
              selection criteria. For example, Bill Days Reef in Espiritu Santo Bay was a dead
              reef in Year I and II and an alternate site at another location was substituted. A
              similar substitution was made for the Hillsborough Bay site in Tampa Bay. South
              Point at East Cote Blanche was completely devoid of living oysters. Since there
              was no alternate location which supported oysters in the East Cote Blanche area,
              this site had to be deleted and no alternate was substituted.
                    Minor shifts in sampling sites have been required between sampling years
              to accommodate yearly fluctuations in the abundance and availability of oyster
              populations at chosen sites. When these minor changes were required, we
              attempted to maintain the sampling stations as near the original location as
              possible. We expect this practice to continue over the course of the study as we
              anticipate recurring problems such as dredge material burying the entire reef
              (CCIC), our inability to relocate our sampling stations on submerged reefs in the
              absence of navigational references or aids in close proximity, the near total
              depletion of public oyster beds by harvesting, disease, and predators (MBLR), and
              the depletion of available oysters attributable to sampling for this study (SAWB
              and TBHB). Thus, in some instances, it has not been possible to return to the
              precise site locations sampled in the previous year. Ensuring continued
              sampling at the same stations for all sites will require the relocation of some of
              the prescribed sites perhaps some distance from the original selected location.
                    The sediment sites were to coincide with oyster sampling sites, inasmuch
              as fine grained depositional sediments may be co-located with the oyster reefs.
              The primary determining factor in sediment sampling was that the sediment
              contain less than 80% sand or coarser fraction. While we have --been unable to
              devise a method which would confirm this compliance in the fiela, every effort
              was made to obtain sediments of as fine a texture as we could subjectively


                                                      24









              determine. In most cases, this was not a problem, as sediments adjacent to the
              reef were indeed of fine texture. However, in some cases, it was difficult (e.g.,
              Bird Island in Charlotte Harbor, Mullet Key Bayou in Tampa Bay or the Naples
              Bay site). These sites, as well as others, were characterized by broad expanses of
              sand and carbonate sediments. Fine grained silts and muds were either
              completely absent or confined to dredged canals used for recreational boating
              quite distant from the oyster collection site. Thus, sediments from some areas
              will not meet the requirement for being more than 20% silt and clay, or they will
              be so far removed from the oyster site that they will not represent the integrated
              contaminant accumulations to which the oysters have been exposed.
                    Comprehensive sediment sampling was undertaken in Years I, II and III
              of the study. However, no sediments were analyzed in Year III. In Year IV
              sediment samples from new Years III and IV sites were analyzed. In Year V,
              only 13 sediment sites were sampled. In Year VI, the two new sites were
              sampled for sediment. Sediment collections in Year VII was confined to five
              newly established sites and PBPH. Sediments were not collected in Year VIII. In
              Year IX, the two new sites, FBFO and FBJB, were sampled for sediments. Table 2
              depicts the sediment and oyster sampling at each site during the nine sampling

              years.


                    Yeam I and H Sampling Summary


                    Two sites (ECSP and ESBD) were completely devoid of oysters. ECSP was
              dropped from further consideration due to the lack of a nearby alternate and no
              chance of oyster populations being restored at the site. CCIC was substituted for
              ESBD, although ESBD was re-checked again in Year II. CCIC's oyster
              population was buried by dredged material in Year II; thus, it too was a non-
              producing site in Year II.
                    The sites at which we had collected oysters in Years I and II, but which
              may not sustain long term collection due to their sparse populations, included
              MBLR, TBIHB, CBSR, and SAWB.
                    Obtaining sediments with less than 80% sand was a problem at a number of
              sites. This was rectified for some sites in Year II by moving further from the
              oyster site and through more diligent searching. Those sites which were unlikely
              to provide sufficiently fine sediments with environmental relevance to the oyster
              site included CBBI, SAWB, NBNB, TBHB, TBMK, TBPB, APCP, and APDB.


                                                      25









                    Year M Sampling Summary


                    In Year III, twenty sites were added to the original list of 51 sampling sites,
              bringing the Year III site total to 71 sites. Sediments and oysters were -collected
              from all of the additional sites, with the exception of LPGO, which had no oysters.
              These new sites are listed below:


                           MBHI    - Hollinger's Island, Mobile Bay
                           CBFM    - Fort Meyers, Charlotte Harbor
                           PBPH    - Public Harbor, Pensacola Bay
                           PCMP    - Municipal Pier, Panama City
                           SRWP    - West Pass, Suwanee River
                           TBOT    - Old Tampa, Tampa Bay
                           BBTB    - Turtle Bay, Barataria Bay
                           CLLC    - Calcasieu Lake, Lake Charles
                           LBNO    - New Orleans, Lake Borgne'
                           LPGO    - Gulf Outlet, Lake Pontchartrain
                           MRPL    - Pass A Loutre, Mississippi River
                           MRTP    - Tiger Pass, Mississippi River
                           ABHI    - Harbor Island, Aransas Bay
                           BRFS    - Freeport-Surfside, Brazos River
                           CCBH    - Boat Harbor, Corpus Christi
                           GBOB    - Offatts Bayou, Galveston Bay
                           GBSC    - Ship Channel, Galveston Bay
                           LMPI    - Port Isabel, Lower Laguna Madre
                           M13CB   - Carancahua Bay, Matagorda Bay
                           MBDI    - Dog Island, Matagorda Bay

                    Four sites in the Lavaca and San Antonio Bay system had no oysters for
              sampling in Year III. Massive die-offs occasioned by excessive freshwater runoff
              into the bays the previous year resulted in no oysters being obtained from the
              SAPP, SAMP, ESSP and MBLR sites. The absence of oysters was confirmed in
              conversations with Texas Parks and Wildlife, who indicated that the die-offs were
              confined to the Matagorda and San Antonio Bays, but that the effects were far
              more widespread than our sampling would indicate. At the same time, oysters
              began to reappear at Bill Days Reef (ESBD), resulting in the first collection of
              oysters from this site.








                                                        26









                             Table 2. Sediment and Oyster Sampling Years I - EK.

                      Station                   yster Year            I         Sediment Year
                                     11 2 3 1 4 1-5 1 6 1 7 1 8 1 9 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9

                 TIDLAS SHES
                      LMSB                      x                          x   x
                      LMPI                   x                     x           x
                      LMAC              I      I   Ix              x      I            x
                      CCNB           x   x   x  XIX         XIX    x   XIX     x
                      ccic           x       x  x x     x   x          x I X--X        x
                      CCBH                   x      x                          x
                      ABLR           x   x   x  x   x   x   x x    x   x x     x   x
                      ABHI              I    X                                 x
                      CBCR           XIX     X  XIX     X I X I       I X -X   X I     x
                      MBAR           x x     x  x x     x   XIX    x XIX       x      I
                      SAPP           x XI       x x     x   xIx    x XIX       x      I
                      SAMP           x   x      x --    x   X1         XIX     x      I
                      ESSP           x   x      x x     x   X1     x  x I x    x
                      ESBD                   XIXIX          xix    x x     x   x
                      MBLR           XIX        x x     x   XIX        x   x   x
                      MB             XIX     x  x       x   XIX    x   x   x   x
                      MBTP           x x     x  x x     x   x x       IX   X   XI
                        'REM         x x     x  x x     x   x   x              XI
                      M                                                x   x   x
                      MBCB                   X      X           x  x           x
                      MBDI                   x                                 x
                      BRCL              I      1XIX     XIX     x  x               x x
                      BRFS                   x  XIX     x   X   X              x
                      GBYC           x   x   x  xjx     x   x   x      X   X   X1      x     I
                      GBTD           x   x   x  XIX     x   x   x  x   x   X- x        x
                      GBHR           x   x   x  XIXIX       x   x      x   x x
                      GBCR           XIX     X  XIX X       XIX       IX   X   X       x
                      GBOB                   x  XIX x       x x    x           X
                      GBSC                   x  XIX     x   x x    x           x       x
                                                   I
              LOUISIANA SITES
                      SLBB           XIX     X  X   XIX     XIX    X   X   X   X1
                      CLSJ           x x     x  x   xix     xix        x   x   x
                      CLLC                   x  x   x x     XIX    x           x
                      JHJH           X XIX      x   x x     x x        x   x   x
                      VBSP           x x x      XIX x       x x    x   x   x I x
                      ECSP                     I   I                  I x  x I    I
                      ABOB                   xlxlxlx        x              xlxl_
                                                                       @x


                                                       Z7











                                                Table 2. Cont.

                     Station                   Oyster Year                      Sediment Year
                                     1   2 13 1 4 1 5   6 1 7   8   9    1  2  3 14    5   6 17 1 8_1 9
                     CLCL            x XIX      XIX     x x     x   x  x x X1          -      I
                     TBLB            x x x      x x     x x     x      x x x                  I
                     TBLF            x   x x    x x     x x     x   x  x    x x
                     BBSD            x   x x    X x     x       x      x    x x
                     BBMB            x   XIX    X--X    XIX     x I   Ix    x x
                     BBIB                   x                                  x
                     MRTP                   x   x x     x x     x              XI
                     MRPL                   x   x x         x   x   x          X1
                     BSBG                x  X   XIX     X   XIX        x    x  x              I
                     BssI            XIXIX      x x     X   X X     XIX     X  X
                     LBMP            x x x      x   x   x   x x     x x     x  x       x
                     LBNO                                           x          x
                     LPGO                               X                                  X


                 NUSSISSEPPI
                     SUES
                     Mspc            X   XIX    X       x       x   X  XIX     X      I       I
                     M,9'RB          XIX X      XIX xf          x      XIX     x      I
                     M
                         'n'D
                        SPB          x x    x   x x     x x x       x  x x     x      I

               AT ABAMA SITES                  I
                     MBDR                           IX         I      I   I            x      I
                     MBCP            x x x      x x     x xix       x  x    x  x                  I
                     MBHI                   x   x x     x XIX       x          1XI     x
                                       I
               FLORIDA SHES            I
                     P EB HIB        x   x  XIX         X X X       XIX     X  X
                     PBPH                   XIX     X   XIX X       x          x              1XI
                     PBSP                           x           X-                     x
                     CBSR            x   x  x x     x   x   x   x      x    x  x       x
                     CBBL              I           I        XI      X                         1XI
                     CBPP            x   xix    x   x   x   XIX     x  x    x  x       x          I
                     CIIRB                                  XI      X1                          XI
                     CBJB                       x   T x     x   x   x                  x          I
                     PCLO                       x   x   x   x   x   x              x   x
                     PCMP                   X   XIX             x           x          x
                     SAWB            XIX    x   XIX     x   x   x      x    x  x       x
                     APDB            x x    X   XIX     XIX     x      x    x  x
                     APCP            X X    X   XIX     X X     X     1XIX     XI
                     AESP                       XIX     x x     x   x              x
                                                    x -                        x
                     SRW                    x       XE
             I       CKBP           1XIX    x &Xx       X XIX-x-               x

                                                       28











                                                    Table 2. Cont.

                        Station                    Oyste Year                         Sediment Year
                                      11121314151617181911121314151617 819
                        TBOT                    X X X       X      I X I X   -  I  I X
                        TBPB                 X  X X X       X   X X      X   X   X X
                        TBHB            X X     X                    X   X   X   X   X
                        TBCB            X X     X   X X     X   X    X       X   X   X
                        TBMK            XjX     XIXIX       X   XIX      X   X   X   X
                        TBKA                        XIX     X   XIX      X1     I       Ix   X      I
                        TBNP                        XIX     X   X X      X               X          I
                        CBBI            X X     X   XIX     X   X    X   X   X   X   X
                        CBFM                    X   XIX     X   X    -9              X
                        NBNB            XIX     X   X X     X   X    X       X   X   X   X   X
                        RBHC            XIX     X   X X     XIX      XjXjX       XjXj
                        FBFO                                             X                                    X
                        FBJB                                             X                                    X
                        EVFU            X    X  XIXIX       X   X    X       X   X   X
                        BBEF                                X   X        X                       X


                  PUERTO RICO
                        PRBB                                    X X      X                           X
                        PRBM                                    X- X     X                          1XI
                        PR"RJ                                  IX X      X1     I                   1XI     I _j

                      The new site established in Louisiana at Lake Pontchartrain (LPNO) was
                totally devoid of oysters and could not be established as a site in Year III.
                Problems in finding sufficient oysters at the Alafia River (TBHB) site in Tampa
                Bay continued in Year III to the point that all oysters at the site were collected, but
                not enough were present for all replicates. It was recommended that this site be
                removed or deleted in Year IV because of the continued problem in finding
                sufficient oysters.


                      Year IV Sampling Summary


                      In Year IV, a total of 67 sites were sampled.       The sites sampled included
                the original list of sites in Years I and II, some sites first sampled in Year III,
                and seven new sites that were sampled for the first time in Year IV. Sediments
                and oysters were collected from all of the new Year IV sites except one.
                      Those sites deleted from and added to the Year IV sampling are listed
                below.



                                                           29









              Sites Deleted from Sampling

                    LMPI     - Port Isabel, Lower Laguna Madre, Texas
                    CCBH     - Boat Harbor, Corpus Christi, Texas
                    ABHI     - Harbor Island, Aransas Bay, Texas
                    MBDI     - Dog Island, Matagorda Bay, Texas
                    MBCB     - Carancahua Bay, Matagorda Bay, Texas
                    ECSP     - South Point, East Cote Blanche, Louisiana
                    BBTB     - Turtle Bay, Barataria Bay, Louisiana
                    LPGO     - Gulf Outlet, Lake Pontchartrain, Louisiana
                    LBNO     - New Orleans, Lake Borgne, Louisiana
                    SRWP     - West Pass, Suwanee River, Florida
                    TBHB     - Hillsborough Bay, Tampa Bay, Florida

              Sites Added- to the Sampling

                    LMAC     - Arroyo Colorado, Lower Laguna Madre, Texas
                    BRCL     - Cedar Lakes, Brazos River, Texas
                    AESP     - Spring Creek, Apalachee Bay, Florida
                    PCLO     - Little Oyster Bay, Panama City, Florida
                    CBJB     - Joe's Bayou, Choctawhatchee Bay, Florida
                    TBKA     - Peter OKnight Airport, Tampa Bay, Florida
                    TBNP     - Navarez Park, Tampa Bay, Florida

                    Three   of the Texas sites, which had no oysters in Year III due to the
              freshwater induced die-off, again had no oysters for collection in Year IV. These
              sites were in San Antonio and Espiritu Santo Bays (SAPP, SAMP, ESSP).
              Extensive effort was made to sample the sites, but only a few spat, too small and
              too few for collection, were found. Indications were good that the populations
              would recover sufficiently for Year V sampling. In Year III, the Lavaca River
              site (MBLR) also had no oysters and it was moved in Year IV to a more favorable
              position.
                    The new site designated in the lower Laguna Madre at the Arroyo Colorado
              also yielded no oysters. Further surveys around Port Mansfield also came up
              without oysters. Thus, it was not possible to sample from the new site designated
              in the lower Laguna Madre.
                    Oysters from three stations were collected at all sites where there were
              oysters except at the Pass a Loutre site on the Mississippi River (MRPL). Two and
              a half hours of dredging did not provide sufficient samples for three replicate sites
              of 20 individuals per site. This low productivity, adverse and worsening weather,
              and one total engine failure combined to result in a short site' (not enough
              replicates for all analyses).



                                                       30









                     Year V Sampling Summary


                     Seventy-one sites were sampled in Year V. The original list of Years I and
               II sites, as well as sites established in Years III and IVI and four new sites were
               sampled for Year V. Sediments and oysters were collected from all of the new
               Year V sites.
                     Those sites deleted from and added to the Year V sampling are listed below.


               Sites Deleted from Samplin

                     LMPI    - Port Isabel, Lower Laguna Madre, Texas
                     ABHI    - Harbor Island, Aransas Bay, Texas
                     MBDI    - Dog Island, Matagorda Bay, Texas
                     ECSP    - South Point, East Cote Blanche, Louisiana
                     BBM     - Turtle Bay, Barataria Bay, Louisiana
                     LBNO    - New Orleans, Lake Borgne, Louisiana
                     LPGO    - Gulf Outlet, Lake Pontchartrain, Louisiana
                     SRWP    - West Pass, Suwanee River, Florida
                     TBHB    - Hillsborough Bay, Tampa Bay, Florida
                     PBIB*   - Indian Bayou, Pensacola Bay, Florida
                     MBGP*- Gallinipper Point, Matagorda Bay, Texas
                     SAMP*- Mosquito Point, San Antonio Bay, Texas

                no live oysters found

               Sites Added to the Sampling

                     M13CB     Carancahua Bay, Matagorda Bay, Texas
                     CCBH* - Boat Harbor, Corpus Christi, Texas
                     MBDR - Dog River, Mobile Bay, Alabama
                     MBPG - Great Point, Mobile Bay, Alabama
                     PBSP - Sabine Point, Pensacola Bay, Florida

                replacement sites for sites without oysters.

                     Two of the Texas sites had no oysters in Year V and were not collected.
               These sites were in San Antonio and Matagorda Bays (SAMP and MBGP). Sites
               sampled in place of these two sites were MBCB and CCBH. Two sites in San
               Antonio and Espiritu Santo Bays (SAPP and ESSP) were sampled for the first time
               in two years.





                                                       31









                   In Year IV, an unsuccessful attempt was made to establish a site in the
             Laguna Madre at the Arroyo Colorado. In Year V, oysters were located and the
             site was sampled.
                   One new site was established in Mobile Bay north of the Dog River .(MBDR).
             An attempt was made to locate a new site on the east side of the bay at Great Point
             (MBPG). However, no oysters could be found, so the site was not established.
                   Another new site in Florida was established at Sabine Point in Pensacola
             Bay south of both of the existing sites. The northernmost site in Pensacola Bay at
             Indian Bayou (PBIB) yielded no live oysters.


                   Year VI Sampling Summary


                   Sixty-four sites were sampled in Year VI. The original list of Years I and
             II sites were sampled, as well as sites established in Years III and IV. Two new
             sites were established for Year V1. Oysters were collected from all of the new
             Year VI sites, but sediments were only collected at the two new sites.
                   Those sites deleted from and added to the Year VI sampling are listed
             below.


             Sites Deleted from Sampling

                   LMPI - Port Isabel, Lower Laguna Madre, Texas
                   LMAC - Arroyo Colorado, Lower Laguna Madre,Texas
                   CCNB*- Neuces Bay, Corpus Christi.Texas
                   CCBH*- Boat Harbor, Corpus Christi, Texas
                   ABHI    - Harbor Island, Aransas Bay, Texas
                   ESBD*   - Bill Days Reef, Espiritu Santo, Texas
                   M13CB   - Carancahua Bay, Matagorda Bay, Texas
                   MBDI    - Dog Island, Matagorda Bay, Texas
                   ECSP    - South Point, East Cote Blanche, Louisiana
                   BBTB    - Turtle Bay, Barataria Bay, Louisiana
                   LBNO    - New Orleans, Lake Borgne, Louisiana
                   MRPL    - Pass A Loutre, Mississippi River, Louisiana
                   MBDR    - Dog River, Mobile Bay, Alabama
                   PBSP    - Sabine Point, Pensacola Bay, Florida
                   PCMP    - Municipal Pier, Panama City,Florida
                   SRWP    - West Pass, Suwanee River, Florida
                   TBHB    - Hillsborough Bay, Tampa Bay, Florida

                    no live oysters found




                                                     32









              Sites Added to the Sampling

                    LPGO* - Gulf Outlet, Lake Pontchartrain, Louisiana
                    BHKF* - Bahia Honda Key, Florida

                     replacement sites for sites without oysters.

                    Two of the Texas sites scheduled for collection , CCNB and ESBD, had no
              oysters in Year VI and were not collected. CCBH was checked as an alternate site
              but it was also devoid of oysters. MRPL was not collected due to sparse collections
              there last year and extremely high water levels in the Mississippi River during
              the sampling period.
                    Two new sites were established this year. A previous attempt to collect
              oysters at Lake Pontchartrain had been unsuccessful. But this year a new site in
              Louisiana, LPGO, was established in the lower part of Lake Pontchartrain. A
              second new site was established in the Florida Keys. Oysters were not present in
              the Keys, therefore an alternate bivalve species (Jewel Box, Chama sinuosa) was
              collected for analysis.


                    Year VIII Samphng Summary


                    Sixty-seven sites were sampled in Year VII. The original list of Years I
              and II sites were sampled, as well as some sites established in Years III and IV.
              Two new sites in Florida and three new sites in Puerto Rico were established in
              Year VII. Oysters were collected from most of the sites that were sampled during
              Year VI. Sediments were collected only at the five new sites and one old station in
              Florida (PBPH).
              below. Those sites deleted from and added to the Year VII sampling are listed

              Sites Deleted from Samplin

                    LMPI    - Port Isabel, Lower Laguna Madre, Texas
                    LMAC    - Arroyo Colorado, Lower Laguna Madre, Texas
                    CCBH    - Boat Harbor, Corpus Christi, Texas
                    ABHI    - Harbor Island, Aransas Bay, Texas
                    MBCB    - Carancahua Bay, Matagorda Bay, Texas
                    MBDI    - Dog Island, Matagorda Bay, Texas
                    ECSP    - South Point, East Cote Blanche, Louisiana
                    BBTB    - Turtle Bay, Barataria Bay, Louisiana



                                                      33










                    BBSD* - Bayou St. Denis, Barataria Bay, Louisiana
                    LBNO - New Orleans, Lake Borgne, Louisiana
                    LPGO - Gulf Outlet, Lake Pontchartrain, Louisiana
                    MSPC* - Pass Christian, Mississippi Sound, Mississippi
                    MSBB* - Biloxi Bay, Mississippi Sound, Mississippi
                    MBDR    - Dog River, Mobile Bay, Alabama
                    PBSP    - Sabine Point, Pensacola Bay, Florida
                    PCMP    - Municipal Pier, Panama City, Florida
                    SRWP    - West Pass, Suwanee River, Florida
                    TBHB    - Hillsborough Bay, Tampa Bay, Florida
                    TBOT    - Old Tampa Bay, Tampa Bay, Florida
                    *no live oysters found

              Sites Added to the Samph

                    CBBL - Bens Lake, Choctawhatchee Bay, Florida
                    CBBB    - Boggy Bayou, Choctawhatchee Bay, Florida
                    PRBB    - Bahia De Boqueron, Puerto Rico
                    PRBM    - Bahia Montalva, Puerto Rico
                    PRBJ    - Bahia De Jobos, Puerto Rico


                    One of the Louisiana sites (BBSD) and two Mississippi sites (MSPC and
              MSBB) scheduled for collection had no oysters in Year VII and were not sampled.

                    Year VM Sampling Summary

                    Sixty-eight sites were sampled in Year VIII. The original list of Years I
              and II sites were sampled, as well as sites established in Years II, IV, and VII.
              Most of the sites sampled in Year VII were sampled again this year. Exceptions
              were when oysters were not present and substitute sites were sampled.
              Sediments were not collected at any sites.

                    Those sites deleted from and added to the Year VIII sampling are listed
              below.

              Sites Deleted from Sampling

                    LMPI - Port Isabel, Lower Laguna Madre, Texas
                    LMAC - Arroyo Colorado, Lower Laguna Madre, Texas
                    CCBH - Boat Harbor, Corpus Christi, Texas
                    CCIC* - Ingleside Cove, Corpus Christi, Texas
                    CBCR* - Copano Reef, Copano Bay, Texas
                    SAMP*- Mosquito Point, San Antonio Bay, Texas
                    ESSP* - South Pass Reef, Espititu Santo, Texas


                                                     34









                    ABHI    - Harbor Island, Aransas Bay, Texas
                    MBDI    - Dog Island, Matagorda Bay, Texas
                    ECSP    - South Point, East Cote Blanche, Louisiana
                    BBTB    - Turtle Bay, Barataria Bay, Louisiana
                    LBNO    - New Orleans, Lake Borgne, Louisiana
                    LPGO    - Gulf Outlet, Lake Pontchartrain, Louisiana
                    MBDR - Dog River, Mobile Bay, Alabama
                    CBBL    - Ben's Lake, Choctawahatchee Bay, Florida
                    CBBB    - Boggy Bayou, Choctawahatchee Bay, Florida
                    SRWP    - West Pass, Suwannee River, Florida
                    BHEF    - Bahia Honda Key, Florida

              *no live oysters found

              Sites Added to Sampling

                    MBCB    - Carancahua Bay, Matagorda Bay, Texas
                    BBSD    - Bayou St. Denis, Barataria Bay, Louisiana
                    MSPC    - Pass Christian, Mississippi Sound, Mississippi
                    MSBB    - Biloxi Bay, Mississippi Sound, Mississippi
                    MBDR - Dog River, Mobile Bay, Alabama
                    PBSP    - Sabine Point, Pensacola Bay, Florida
                    PCMP    - Municipal Pier, Panama City, Florida
                    TBBB    - Hillsborough Bay, Tampa Bay, Florida
                    TBOT    - Old Tampa Bay, Tampa Bay, Florida

                    Four of the Texas sites (CCIC, CBCR, SAMP,         and ESSP) scheduled for
              collection had no oysters in Year VIII and were not sampled.


                    Year IX SampUng Summary


                    In Year IX, a total of 55 sites were visited and 51 of them successfully
              sampled. Four of the originally scheduled sites were devoid of any live oysters, so
              four alternate sites were visited and sampled. Oysters were collected from a
              range of sites that were originally established in Year I through Year VII. Two
              new sites were established for Year IX, at Flamingo in Florida Bay (FBFO), and
              in Joe Bay in Florida Bay (FBJB), where both sediments and oysters were
              collected. No other sediments samples were taken this year.
                    The list of sites sampled in Year IX is shown below, along with lists
              showing the deletions and additions to the previous year's sampling list.






                                                      35









             Year IX Sampling Sites

                   LMSB -     South Bay, Lower Laguna Madre, Texas
                   LMPI -     Port Isabel, Lower Laguna Madre, Texas
                   LMAC -     Arroyo Colorado, Lower Laguna Madre, Texas
                   CCNB -     Nueces Bay, Corpus Christi, Texas
                   ABLR -     Long Reef, Aransas Bay, Texas
                   MBAR -     Ayres Reef, Mesquite Bay, Texas
                   SAPP    -  Panther Point Reef, San Antonio Bay, Texas
                   ESSP    -  South Pass Reef, Espiritu Santo, Texas
                   ESBD    -  Bill Day's Reef, Espiritu Santo, Texas
                   MBGP    -  Gallinipper Point, Matagorda Bay, Texas
                   MBCB    -  Caancahua Bay, Matagorda Bay, Texas
                   BRCL    -  Cedar Lake, Brazos River, Texas
                   GBTD    -  Todd's Dump, Galveston Bay, Texas
                   GBOB    -  Offatts Bayou, Galveston Bay, Texas
                   GBSC    -  Ship Channel, Galveston Bay, Texas
                   SLBB    -  Blue Buck Point, Sabine Lake, Louisiana
                   CLLC    -  Lake Charles, Calcasieu Lake, Louisiana
                   VBSP    -  Southwest Pass, Vermilion Bay, Louisiana
                   CLCL    -  Caillou Lake, Caillou Lake, Louisiana
                   TBLF    -  Lake Felicity, Terrebonne Bay, Louisiana
                   MRPL    -  Pass a Loutre, Mississippi River, Louisiana
                   BSSI    -  Sable Island, Breton Sound, Louisiana
                   LBMP    -  Malheureux Point, Lake Borgne, Louisiana
                   LBNO    -  New Orleans, Lake Borgne, Louisiana
                   MSPC    -  Pass Christian, Mississippi Sound, Mississippi
                   MSPB    -  Pascagoula Bay, Mississippi Sound, Mississippi
                   MBCP    -  Cedar Point Reef, Mobile Bay, Alabama
                   MBHI    -  Hollingers Island Channel, Mobile Bay, Alabama
                   PBPH    -  Public Harbor, Pensacola Bay, Florida
                   PBIB    -  Indian Bayou, Pensacola Bay, Florida
                   CBJB;   -  Joe's Bay, Choctawahatchee Bay, Florida
                   CBBL    -  Ben's Lake, Choctawahatchee Bay, Florida
                   CBPP    -  Postil Point, Choctawahatchee Bay, Florida
                   CBBB    -  Boggy Bayou, Choctawahatchee Bay, Florida
                   PCLO    -  Little Oyster, Panama City, Florida
                   AESP    -  Spring Creek, Apalachee Bay, Florida
                   CKBP    -  Black Point, Cedar Key, Florida
                   TBOT    -  Old Tampa Bay, Tampa Bay, Florida
                   TBPB    -  Papys Bayou, Tampa Bay, Florida
                   TBHB    -  Hillsborough Bay, Tampa Bay, Florida
                   TBMK    -  Mullet Key Bayou, Tampa Bay, Florida
                   TBKA    -  Knight Airport, Tampa Bay, Florida
                   TBNP    -  Navarez Park, Tampa Bay, Florida
                   CBBI    -  Bird Island, Charlotte Harbor, Florida
                   RBHC    -  Henderson Creek, Rookery Bay, Florida
                   FBFO    -  Flamingo, Florida Bay, Florida
                   FBJB    -  Joe Bay, Florida Bay, Florida



                                                    36










                    BHEF - Bahia Honda Key, Florida
                    PRBB - Bahia de Boqueron, Puerto Rico
                    PRBM - Bahia Montalva, Puerto Rico
                    PRBJ - Bahia de Jobos, Puerto Rico

             Sites deleted from the Sampling List

                    CCIC*   -  Ingleside Cove, Corpus Christi, Texas
                    CCBH    -  Boat Harbor, Corpus Christi, Texas
                    ABHI    -  Harbor Island, Aransas Bay, Texas
                    CBCR*      Copano Reef, Copano Bay, Texas
                    SAMP    -  Mosquito Point, San Antonio Bay, Texas
                    MBLR    -  Lavaca River Mouth, Matagorda Bay, Texas
                    MBTP    -  Tres Palacios Bay, Matagorda Bay, Texas
                    MBEM -     East Matagorda, Matagorda Bay, Texas
                    MBDI    -  Dog Island, Matagorda Bay, Texas
                    BRFS    -  Freeport Surfside, Brazos River, Texas
                    GBYC    -  Yacht Club, Galveston Bay, Texas
                    GBHR    -  Hanna's Reef, Galveston Bay, Texas
                    GBCR    -  Confederate Reef, Galveston Bay, Texas
                    CLSJ    -  St. John's Island, Calcasieu Lake, Louisiana
                    JHJH    -  Joseph Harbor Bayou, J.H. Bayou, Louisiana
                    ECSP    -  South Point, East Cote Blanche, Louisiana
                    ABOB    -  Oyster Bayou, Atchafalaya Bay, Louisiana
                    TBLB    -  Lake Barre, Terrebonne Bay, Louisiana
                    BBSD    -  Bayou St. Denis, Barataria Bay, Louisiana
                    BBMB    -  Middle Bank, Barataria Bay, Louisiana
                    BBTB*   -  Turtle Bay, Barataria Bay, Louisiana
                    MRTP    -  Tiger Pass, Mississippi River, Louisiana
                    BSBG    -  Bay Gardene, Breton Sound, Louisiana
                    LPGO* -    Gulf Outlet, Lake Pontchartrain, Louisiana
                    MSBB    -  Biloxi Bay, Mississippi Sound, Mississippi
                    MBDR -     Dog River, Mobile Bay, Alabama
                    PBSP    -  Sabine Point, Pensacola Bay, Florida
                    CBSR    -  Off Santa Rosa, Choctawahatchee Bay, Florida
                    PCMP    -  Municipal Pier, Panama City, Florida
                    SAWB    -  Watson Bayou, St. Andrew Bay, Florida
                    APDB    -  Dry Bar, Apalachicola Bay, Florida
                    APCP    -  Cat Point Bar, Apalachicola Bay, Florida
                    SRWP    -  West Pass, Suwanee River, Florida
                    TBCB    -  Cockroach Bay, Tampa Bay, Florida
                    CBFM    -  Fort Meyers, Charlotte Harbor, Florida
                    NBNB    -  Naples Bay, Naples Bay, Florida
                    EVFU    -  Faka. Union Bay, Everglades, Florida

                     no live oysters found









              Sites Added to the Sampling List

                     LMPI    -   Port Isabel, Lower Laguna Madre, Texas
                     LMAC    -   Arroyo Colorado, Lower Laguna Madre, Texas
                     CCIC    -   Ingleside Cove, Corpus Christi, Texas
                     ABLR    -   Long Reef, Aransas Bay, Texas
                     CBCR    -   Copano Reef, Copano Bay, Texas
                     ESSP    -   South Pass Reef, Espiritu Santo, Texas
                     BBTB    -   Turtle Bay, Barataria Bay, Louisiana
                     LBNO    -   New Orleans, Lake Borgne, Louisiana
                     LPGO    -   Gulf Outlet, Lake Pontchartrain, Louisiana
                     CBBL    -   Ben's Lake, Choctawahatchee Bay. Florida
                     CBBB    -   Boggy Bayou, Choctawahatchee Bay. Florida
                     FBFO    -   Flamingo, Florida Bay, Florida
                     FBJB    -   Joe Bay, Florida Bay, Florida
                     BHEF    -   Bahia Honda Key, Florida


                     Two Texas sites (CCIC & CBCR) and two Louisiana sites (BBTB & LPGO)
              that were scheduled for collection in Year IX, had no live oysters and were not
              sampled. The two Texas sites have not had any live oysters for two years in a row
              now, and it may indicate that these reefs are now dead. Four alternate sites were
              selected and sampled; CCNB for CCIC, MBAR for CBCR, MBCP for BBTB and
              LBNO for LPGO.
                     The Galveston Bay Ship Channel site (GBSH) had very few live oysters this
              year. The reef has been covered with a fine layer of silt, coming from the
              surrounding ongoing landfill operations. The Pass a Loutre site on the
              Mississippi River (MRPL) only had a very small patch of live oysters. Local
              fishermen have commented on the declining oyster population in the area over
              the past few years. This could be as a result of increased fresh water from the
              Mississippi River flowing down Pass a Loutre and into Redfish Bay. Veteran's
              Pier (MSPC), at Pass Christian, was re-occupied this year as oysters on the site
              were once again plentiful. The oysters here are affected by the cold water and
              weather, and numerous frozen shells on the site supported the fact. The
              Pascagoula Bay site (MSPB), in the Mississippi Sound, was moved approximately
              half a mile to the east, to the western side of the Navy Base Island. There were no
              live oysters to be found at the old site, as the area has been covered by a thick layer
              of fine sand. There were problems in finding sufficient oysters in the Alafia River
              site in Tampa Bay ( TBHB), and the site was moved to the western/@outhern. side of
              the river where the oysters were somewhat more plentiful.




                                                        38











             References


             Parker, R.H. (1960) Ecology and distributional patterns of marine macro-
                   invertebrates. Northern Gulf of Mexico by F.P. Shepard, F.B. Phleger, and
                   T.H. van Andel in Recent Sediment, Northwest Gulf of Mexico. A
                   Symposium Summarizing the Result of Work Carried on in Project 51 of the
                   American Petroleum Institute 1951-1958.            Scripps Institute of
                   Oceanography, University of California, La Jolla, California.

             Lawrence, D.R. and G.I. Scott (1982) The determination and use of condition
                   index of oysters. Estuaries, 5:23-27.






































                                                    39









             Gulf of Me3deo and Caribbean Site Descriptions


                   Location of Gulf of Mexico and Caribbean NS&T Mussel Watch sampling
             sites are shown in Figures 5 through 18. Detail descriptions for each individual
             site follow. For each site, the following components are included:


                   Detailed description

                   Road map to the site

                   Nautical chart of the site

                   Photographs of the site.  Photographs, when available, are arranged in a
                   clockwise manner starting in the upper left hand corner with an aerial
                   view and then Stations 1, 2 and 3, respectively.
































                                                    40




















                                                                                                      gel                                                94.                       92-                       go.                                                  Be.                       84-                         82-                      80.

                                                                                                 34-







                                                                                                 32


                                                                                                                                                                                                                                          Fig. 12.':
                                                                                                                                                                                                                         M
                                                                                                                                                                                                                             obile


                                                                                                                                                                                                   New
                                                                                                                                                                                                  Orleans'.:".                                                                 Z.
                                                                                                                                                                                                                                              Fig. 13
                                                                                                                        Galvest         n"
                                                                                                                                                                    Ig
                                                                                                                                                                                                                                                                        Fig. 14
                                                                                                                                                                                                                                                                                                                              Cops'.
                                                                                                                                         Fig. 9                                            Fig. 11                                                                                                                          Cons"
                                                                                                 28-                                                                                                                                                                                                                Tamps-
                                                                                                      V"                Fig. 8
                                                                                                                                                                                                                                                                                              Fig. 15


                                                                                                 28-
                                                                                                                Fig.                                                                                                                                                                                        F1          16

                                                                                                                                                                                                                                                                                                           too athoms


                                                                                                 24
                                                                                                                                                                                                                                                                                                                                Fig. 17
                                                                                                                                                                                                                                         __Fig-
















                                                                                                      1A
                                                                                                                                                        94.                       92-                        go.                       ge.                        86.                       a'.                         821*                      80


                                                                                                                                                                        Figure 6.                         Location of Gulf of Mexico Sampling Sites.











                                                                  The Bahamas                                            ATLANTIC
                                                     .0                                                                      OCEAN
                                                      0             %
                                                                         10


                                                                                   Turks and Caicos
                                                                                                                Puerto British Virgin Is.
                                                                                                                  Rico
                                                                                                                             44            4P
                                                                                                                               A
                                                                                                                             .s. Virgin    %
                                                     4@ft                                                   Fig
                                                                                 CARIBBEAN                                                             C. Marti
                                                                                          SEA
                                                                                                                                                           jar




                                                                                                                                        ab                    TO
                                                                                                                                                            & T
                                                                                                                                           0,@ mnlm.



                                                                          Figure 6.            Location of Caribbean Sampling Sites.






                                                                       78    1 - Laguna Madre South Say (LMSS)             1A
                                                                   @@_;52 - Laguna Madre Port Isabel (LMPI)
                                                                           78 - Laguna Madre Arroyo CA)Ioredo (LMAC)




                                                                     D








                                                                                                  LAGUNA


                                                                                                   MADRE


                                                                                                                                      CL









                                                    AW"




















                                                                                                                             52

                                                               LAGUMALAAGA
                                                                                                        oft.

                                                                                   LAGWA
                                                                                                                                   CLt
                                                                                                                    W-W            k4-W
                                                                                   MADRE            1,AD/A
                                                                                                   AUCHA




                                                                                                                                 ar
                                                                                                                                   azo-l-41

                                                             SAW GRANDE
                                                                                                                      SOUTH       SAY




                               Figure 7.               Location of Laguna                    Madre South               Bay Sites (see Figure 5 for
                                                       location in the Gulf of Mexico).


                                                                                               43














                                                                                                                                           '13
                                                                                                               A 1
                                                TEXA S                                                                                            55

                                                                                                                12
                                                                                                                                         0  Fk
                                                                                                                          A4 A  T A
                                                                                                                                                              G



                                                                                                     SEADRIFT
                                                                                                                       0
                                                                                                                                                             M   E

                                                                                                   N
                                                                                               A T NIO
                                                                                                                        2    Corpus Christi Neuces Say (CCNB)
                                                                                                                        3    Corpus Christi Ingleside Cove (CCIC
                                                                                                                        4    Aransas Bay Long Reef (ABLR)
                                                                           _k
                                                                                          6
                                                                        0                                               6    Copeno Say Copano Reef (CBCA)
                                                                   COPAO                N-
                                                                               4                                        6    Mesquite Say Ayres Reef (MBAR)
                                                                 ROCKPORT            P                                  7  - San Antonio Bay Panther Point Reef
                                 .28'
                                                                                                                        8  - Son Antonio Bay Mosquito Point (SA
                                                                                                                        9  - Espiritu Santo South Pass Reef (ESS
                                                        PORnAND.            0                                          10  - Espiritu Santo Bill Days Reef (ESSO)
                                                  2                                                                    11  - Matagorda Say Lavaca River Mouth
                                            NIJE6   1
                                              a        3 3                   post                                      12  - Matagorda Say GaIIInIpper Point (M
                                          con
                                          CHRIS I     CORPUS                                                           13  - Matagorda Bay Tres Palaclos Say (h
                                                        CHRISTI                                                        14  - Matagorda Say East Matagorda (MB
                                                             SAY                                                       53  - Corpus Christi Boat Harbor (CCBH)
                                                                                                                       54  - Aransas Bay Harbor Island (ABHI)
                                                                                                                       55  - Matagorda Bay Dog Island (MBDI)
                                                                                                                       56  - Matagorda Say Carancahus Bay (M



                                                     Figure 8.          Location of South Texas Sites (see Figure 5 for location in t
                                                                        of Mexico).
























                                                                                                                            TEXAS

                                  HOUSTON


                                                                                                           4b

                                                                                                        A

                                                    o SOUTH         LA ,Cmim
                                                      HOUSTON

                                                        0 GENOA


                                                                                          IV,
                                                                                              ON
                                                                                              0                    E ,SI

                                                                                 SAN







                                                                                  TEXAS MY






                                                                                                       GALVESTON
                                                                                    8






                                  *ANGUETON
                                                                  WES
                                                                                          15     Galveston Say Yacht Club (GOYC)
                                                                                                 Galveston Say Todd's Dump (GSTO)
                                                                                          17  -  Galveston Say Hanna Red (GSHR)
                                                                                          18  -  Galveston Say Confederate Red (GSCR)
                                                                                          57  -  Freeport Surtside (SRFS)
                                                                                          58  -  Galveston Say Offats Bayou (GSOB)
                                                                                          5 9 -  Galveston Say Stup Channel (GBSC)
                                        P@O>f"%T
                                                                                              - Brazos Rivor Codar Lake (ORCL)
                                                                                          72
                                  7
                                             57





                          Figure 9.             Location of Galveston Bay Sites (see Figure 5 for location in the
                                                Gulf of Mexico).



                                                                                       45



















                                                                                           93,
                               TEXAS                 LOUISIANA-                  Lake Charles



                                      Orange                                0


                        .30.




                        Port Arthu
                                                                                                              Of.






                                                                                                        21



                         29,301       19 - Sabine, Like Blue Suck Point (91.68)
                                      20 , Calcasieu Lake St. John& Island (CLSJ)
                                      21 - Joseph Harbor Joseph Harbor Bayou (JHJH)
                                      22-  Vermillion Bay Southwest Post (VBSP)
                                      60 - Calceslou Lake Lake Charles (CLLC)                    GULF     OF    MEXICO



                                           Figure 10. Location of Western Louisiana Sites (see Figure 5 for location
                                                            the Gulf of Meidco).



















                                                                                                   LAXE
                                                                                                                                   901
                                                                                                 A# VREPAS


                                                                                                              LAKE   PONTCHARTI
                                                                                                                                      .........


                                                                                                                                   MIN
                                                                                                                                                                    &

                                                                                                                                                           0
                                                                                                                                                     J
                                          ioll                                                                                                          4                A
                                          04                                                                      0
                                                                                                                                              62

                                                                                                                                                '32



                                                                                                                                                                     '4b
                                                                            LOUISIANA
                                                                                                                              61

                                          A rC&,
                                                                                                                                      2 8.


                                                                                                            -27,
                                                         24
                                                                                100.Looxt
                                                                                                                                                              6

                                        29-      24     Atchafalaya Bay Oyster Bayou (ASOB)                       31     Breton Soun  .d Sable Island (BSSI)
                                                 25     Caltiou Lake Calliou Lake (CLCL).                         32     Lake SOWS Malheureux Point (LOMP)
                                                 26     Tarrebonne Say Lake Barre (TOLS)                          61     Barataria Be .y Turtle Say (813TI3)
                                                 27     Tetrebonne Say Lake Felicity (TBLF)                       62     Lake Borgne Now Orleans (LBNO)
                                                 28     Barataria Day Bayou St. Denis (BBSI))                     6 3    Lake Ponchartrain Gulf Outlet (LPGO)
                                                 29     Barataria Say Middle Bank (BOMB)                          64     MISSINDIPPI Rlvdr Pass A Loutre (MRPL)
                                                 30  ,  Breton Sound Say Garderns (88130)"                        65  ,  Mississippi River Tiger Pass (MRTP)
                                                     Figure 11. Location of Eastern Louisiana and Mississippi Delta Sites
                                                                         Figure 5 for location in the Gulf of Mexico).












                                                                    64                  8830-

                                                                                     Cn jr-
                                        33 - mississsippi sound Pass Christian (MSPC) (A 11%
                                                                                     @ IM
                                        34 -Mississippi Sound Biloxi Say (MSBB)
                                        35 -Mississippi Sound Pascagoula Bay (MSPB)
                                        36 -Mobile Bay Cedar Point Reef (MBCP)
                                        66 -Mobile Say Hollinger Island (MBHI)
                     MISSISSIPPI        79 - Mobile Say Dog River (MBDR)



                                                                           P 'to0


                                                                                           MOBIL








                     -30*30'                                                    PASCAGIOULAI
                                    rn
                                                                       34.-


                                                        ASS CHRISTIAN

                          -SLIDELL*
                                                                    X
                        LAKE
                     PONTCHARTRAIN
                                                                      G U L F  0 F   MEXICC
                                           OORGN,

                                                                                           88,30,
                           Figure 12. Location of Mississippi/Alabama Sites (see Figure 5 for location
                                      the Gulf of Mexico).
















                                                                           FLORIDA
                                                   37                           8239.
                                                                              Fort         83.
                                          Pensacola                         walton,",
                                                       pense oil a y
                                                                            Beach'  c"ace-hatch- Bay
                                                                          ZB e,a
                                                                                        3-
                                                                                      7
                                                         80                                                                 74...
                                                                                                                       West sly
                                                                GULF OF MEXICO                                         68
                                                                                                                   Panama


                            30*

                                     37 - Pensacola Bay Indian Bayou (PBIB)
                                     38 - Choctawhatchee Bay Off Santa Rosa (CBSR)   67 - Pensacola Bay Public Harbor (PBPH)
                            -        82 - Choctawhatchee Bay Bens Lake (CBBQ         68 - Panama City Municipal Pier (PCMP)
                                     39 - Choctawhatchee Bay Postil Point (CBPP)     73 - Choctawhatchee Bay Joes Bayou (CBJB)
                                     83 - Choctawhatchee Bay Boggy Bayou (CBBB)      74 - Panama City Little Oyster Bay (PCLO)
                                        - St. Andrew Bay Watson Bayou (SAWB)         80 - Pensacola Bay Sabine Point (PBSP)
                                                      8!-                                                     8p'
                                         Figure 13. Location of Western Florida Sites (see Figure 5 for location in the Gulf
                                                      of Mexico).























                                                                                          A  LL@
                                                                                             SAY
                                                                       'Jormeo








                                     ..........
                              8L





                                                                                  GULF OF MEXICO
                              41 - Apalachicola Say Dry Day (A POS)                                                                    69
                              42- Apalachicola Say Cal Point Bar (APCP)
                              43'- Cedar Key Black Point (CKBP)
                              69- Suwahnee River West Pass (SRWP)
                              75 - Apalachee Bay Spring Creek (AESP)

                                                                                                  01.
                                           Figure 14. Location of Middle Florida Sites (see Figure 5 for location ix
                                                            Gulf of Mexico).













                                                                                                   62:30'

                                                                               44   - Tampa Bay Papys Bayou (TBPO)
                                                                               45   -  Tampa Bay Hillsborough Bay (TBHB)
                                                                               46      Tampa Bay Cockroach Bay UBCB)
                                                                                       Tampa Bay Mullet Key Bayou (TBMK)
                                                                               47
                                                                               70   -  Tampa Bay Old Tampa Bay (TBOT)
                                                      fA
                                                      CD
                                                      '0                                                                   P)
                                                                               76   -  Tampa Bay Navarez Park (TBN
                                                                               77   -  Tampa Bay Knight Airport (TBKA)
                                                      C',


                                                      jCX
                                                           Dunedin. -               .70
                          28-                                                                         F L 0 R I D A               28'

                                                                                                 Tampa
                                                                               0-11




                                                                                                  Port Hillsboroug
                                                                                                            Bay           Aiara mim,
                                                                               44''

                                                                                                    Gadsden Pt

                                                            St Petersbur
                                GULF
                                                      76
                                  OF


                                                                               Pinenas


                               MEXICO
                                                           47W
                                                                                              F.




                                                                  Anna
                                                                4
                                                                  Maria Island
                                                                hz
                                                                           0





                         Figure 15.          Location of Tampa Bay Sites (see Figure 5 for location in the Gulf
                                             of Mexico).

                                                                                 51
















                                        82-                                      81;30.



                         14
                                                      -FORT MEYERS




                                                   71

                   26'30'                                                                 26*30'-




                                 Is, n d


                                                                        F L 0 R I D A






                         GULF    OF MEXICO


                                                           RAPLES.
                                                            .'.49


                   -48 - Charlotte Harbor Bird Island (CBBQ                                 2w-
                     49 - Naples Bay Naples Say (NBNB)
                     50 - Rookery Bay Henderson Creek (RBHC)
                     51 - Everglades Faka Union Say (EVFU)        11 Lpi
                                                                    W-r
                     71    Charlotte Harbor Fort Meyers (CBFM)         CAPE
                                                                  VROMAN




                  Figure 16.    Location of South Florida Sites (see Figure 5 for location in the
                                Gulf of Me3dco).

                                                          52
















                                                                        FLORIDA






                              GULF OF

                               MEXICO


                                                                87


                   25*                                                                                     2z.





















                       81 - Bahia Honda Key Florida (BHKF)
                       87 - Flamingo Florida Bay (FBFO)
                       88 - Joe Bay Florida Bay (FBJB)






                      Figure 17.      Location of Florida Key Site (see Figure 5 for loc'ation in the Gulf
                                      of Mexico).
                                         p
                                    NO








                                                                 53




















                                                ATLANTIC OCEAN




                                                         San Juan
                                                                                              19
                                                                                      C@5 rjo
                                                                                       St.,/06n
                        Mayaguez       PUERTO RICO
                                                                                               t4
                                      8.5.
                           84
                                              Ponc"    86
                      84 - Puerto Rico Bahia De Boqueron (PRBB)                          Croi,

                      85 - Puerto Rico Bahia Montalva (PRBM) CA R I B.B EA N S      EA

                      86 - Puerto Rico Bahia De Jobos (PRBJ)

                                               Figure 18. Location of Puerto Rico Sites.









                                                TEXAS SrIES



              SrIE NUMBER - 1                                DESIGNATOR - LMSB

              SITE - SOUTH BAY, LOWER LAGUNA MADRE, TX

              NOMINAL SITE CENTER - 26002.77'N               97010.48W


              LOCATED ON NOS CE[ART           11302

              SITE ACCESS - A tunnel hull outboard fishing boat and captain are generally
              chartered at the White Sands Marina in Port Isabel. In Year IV, a GERG boat
              was launched at the public boat ramp on South Padre Island at the Isla Blanca
              Pier. Travel time to South Bay is about 25 minutes. Landmarks used to locate the
              reef are 1450 from the middle of the causeway bridge and 900 from the
              navigational range marker. The water depth is very shallow and requires the use
              of a shallow draft vessel.

              SITE DESCRIPTION - The oysters were located in clumps on expansive intertidal
              mud flats south of Clark Island on the northeast side of South Bay and were fully
              exposed at low tide. The dense clumps were mostly conglomerations of dead
              shells. The few live specimens were small, thin-shelled oysters interspersed
              among the empty shells. The reef contained many dead shells, and collecting a
              sufficient number of suitably sized oysters required searching and picking
              through the clumps of shells.

              OYSTERCOLLEMONS

              Years I, II, & III - There was no significant geographic distinction in the three
                     stations. All three stations were taken from the middle of the reef.

              Year IV - Stations were collected in the same general location but were separated.
                     Station 1 was taken at the north end of the reef just south of Clark Island
                     and adjacent to the Spartina alterniflora marsh.            Station 2 was
                     approximately 75 m to the south at two small reefs about 10 m apart.
                     Station 3 was at the south end of the reef, 100 m south of the middle part of
                     the reef.

              Year   V - Oysters were much larger and more abundant than previous years'
                     collections. Stations were on the same reef but were separated. Station 1
                     was on the northeast shoreline. Station 2 was 100-200 m south (toward the
                     duck blind) from Station 1. Station 3 was another 100-200 m south.

              Years  VI, VII, &VIII - Oysters were collected at the same locations as sampled
                     the previous three years.

              Year IX - Oysters were sampled at the same station locations as in Years V
                     through VIII. Oysters were abundant, large and thick shelled, in contrast


                                                       55









                      to the conditions found in Year I of the study. The reef has steadily
                      improved.

                   )DUENTCOLLECTIONS

               Years I, II, & III - Sediments were collected during the first three years of the
                      study. Sediments were taken from the middle (Station 1), west end (Station
                      2), and east end (Station 3) of the reef.

               Years IV, V, VI, VII, VIII, & XI - No sediments were collected during these
                      years.

               SAMPLING METHODS

                      Oysters - hand
                      Sediment - hand

               WATER DEPTH - intertidal, 0.3 m

               POSSEBLE CONTAMINANTS - There were no obvious sources of contamination
               in the vicinity of the reef, although the salinity of the bay was high and the oysters
               endured exposure out of the water for a considerable period during the day.

               ENVIRONM[ENTAL DATA

                                         Salinity           Temperature         Date Sampled
                                            (0/00)               (0c)

                    YEAR 1                  30.0                 18.0          24 January 1986
                    YEAR 11                 30.0                 20.0          9 December 1986
                    YEAR 111                43.0                 13.0          15 December 1987
                    YEAR IV                 37.0                 10.0          13 December 1988
                    YEAR V                  34.0                 10.0          13 December 1989
                    YEAR VI                 40.0                 21.0          12 December 1990
                    YEAR VII                34.0                 25.0          11 December 1991
                    YEAR VIII               34.0                 15.0          16 December 1992
                    YEAR IX                 31.0                 19.5          9 December 1993

















                                                            56





























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                                                        Site #1 (LMSB), Lower Laguna Madre, South Bay.
     31arer Publishing Company. Reprinted by Permission-













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              SITE NUMBER - 52                              DESIGNATOR - LMPI

              SITE - PORT ISABEL, LOWER LAGUNA MADRE, TX

              NOMINAL SITE CENTER - 26004.62'N              97012.05'W


              LOCATED ON NOS CHART # - 11302

              SITE ACCESS - Access to the site is by foot from the parking lot at the Queen's
              Point Marina in Port Isabel.

              SITE DESCRIPTION - This collection site, which was established and collected
              only in Year III, was located along the south jetty of the Queen's Point Marina.
              Oysters grow attached to the concrete bulkhead and are exposed at low tide.
              Numerous shells are present but a high proportion of the oysters found here were
              dead and the live oysters were thin-shelled. Careful handling was required while
              collecting to avoid breaking the live oysters. Typically, salinities are high
              throughout the year. In Year IX, the first resampling of the site since Year III,
              the entire marina facility was found to be gone. All buildings and structures in
              the water had been removed. The concrete bulkheads, from which the oysters
              were collected, were all that remained.

              OYSTER COLLECTIONS

              Year III - Discrete stations were sampled as described below. Station 1 was
                    located on the breakwater extending south from the marina entrance.
                    Station 2 was along the south facing bulkhead. Station 3 was located
                    between Stations 1 and 2 in a cove formed by the two bulkheads.

              Years IV, V, VI, VII, & VIII - The site was not sampled during these years.

              Year IX - The same stations were sampled as in Year III.

              SEDIMENT COLLECTIONS

              Year III - Sediments were collected outside the bulkheaded area opposite Station 1
                    on the side adjacent to the Brownsville Ship Channel.

              Years IV, V, VI, VII, VIII, & X1 - No sediments were sampled for during these
                    years.

              SAMPLING METHODS

                    Oysters - hand
                    Sediments - hand

              WATER DEPTH - Intertidal, 0.25 and 0.5 m.





                                                      61









           POSSIBLE CONTAMINANTS - Contamination of the site was possible from
            nearby marinas, the City of Port Isabel, and the Brownsville Ship Channel.

            ENVIRONMENTAL DATA

                                 Salinity      Temperature     Dates Sampled
                                  (%0)             (OC)

                YEAR 111          42.0             13.0       16 December 1987
                YEAR IX           29.0             21.0       8 December 1993
















































                                               62














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                                                                      Site #52 (LMPI), Laguna Madre Port IsabeR.
      thearer Publishing Company. Reprimed by permission-













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              SITE NUMBER - 78                            DESIGNATOR - LMAC

              SITE - ARROYO COLORADO, LOWER LAGUNA MADRE, TX

              NOMINAL SITE CENTER - 26016.80 rN           97017.30V

              LOCATED ON NOS CHART # - 11302

              SITE ACCESS - Access to the site is by boat launched at the White Sands Marina
              in Port Isabel. Travel up the ICWW to the site. Travel time is 40 minutes.

              SITE DESCRIPTION - This collection site, established and collected for the first
              time in Year V, was located at cabins along the ICWW. Oysters grow attached to
              the concrete pilings and on mud flats which were exposed at low tide.

              OYSTER COLLECTIONS

              Year  V - Station 1 was located at the first cabin #1399 (tan with brown trim),
                    which was just north of channel marker 45 and west of Primero Island.
                    Oysters were collected from the concrete piling supporting the cabin.
                    Station 2 was at cabin #1423. The cabin was between channel marker 41
                    and channel marker 43. Station 3w.as located north of channel marker 41
                    at cabin #P1345. Oysters at this station were growing in clumps on the
                    mudflat. Many were very large with smaller more suitably sized oysters
                    attached.

              Years VI, VII, & VIII - This site was not sampled during these years.

              Year IX - The location of the stations were the same as in Year 'V, Station 1 at
                    cabin #1399, Station 2 at cabin #1423 and Station 3 at cabin #1345. The
                    oysters were fewer on the pilings than in Year V.

                 DIMENTCOLLECTIONS

              Year V - Sediments were sandy silts which were tan on the surface which overlay
                    a black layer mixed with shell fragments and sea grasses. These were
                    collected at the cabins for Station 1 and 2. Station 3 sediments were
                    collocated with the oysters on a subtidal mudflat.

              Years VI, VII, VIII, & IX - No sediments were sampled during these years.

              SAMPLINGMETHODS

                    Oysters - hand
                    Sediments - hand

              WATER DEPTH - intertidal, 0.3 m





                                                     67









            POSSIBLE CONTAMINANTS - Contamination of the site was possible from
            nearby cabins, a producing gas field just to the south of the sampling area and
            inflow from the Arroyo Colorado.

            ENVHtONMENTAL DATA

                                 Salinity      Temperature     Dates Sampled
                                  (TOO)             (OC)

                YEAR V             34.0             11.0      14 December 1989
                YEAR IEK           30.0             22.0       8 December 1993















































                                                68




















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               SrIE NUMBER - 2                               DESIGNATOR - CCNB

               SITE - NUECES BAY, CORPUS CHRISTI, TX

               NONONAL SrIE CENTER - 27-51.17'N               97021.55'W


               LOCATED ON NOS CHART # - 11309


               SITE ACCESS - The site is located at Indian Point Marina on the north end of the
               U.S. Highway 181 bridge that crosses Nueces Bay. There is a boat launch at the
               Gunderlands Boatland. Collection stations are located along the shoreline on the
               north side of the road.

               SITE DESCRIPTION - The site was located 100 m from the boat ramp at bearing
               3250. The bay was very shallow with exposed reefs. These reefs had mainly dead
               oysters. Small thin-shelled oysters were collected along the mud shoreline or on
               pilings. Oysters were intertidal and seemed to be on the decline.

               OYSTERCOLLECTIONS

               Years I, II, & III - Oyster collection stations were as follows: Station 1 - 145' to
                     boat ramp; 213' to the top of the Corpus Christi bridge. Station 2 - 250 to
                     Portland water tower; 2080 to the top of the Corpus Christi bridge. Station 3
                      25' to Portland water tower; 115* to large pilings at Gunderlands.

               Year IV - Oysters were collected in Year IV at the same location as in previous
                     years. The three stations were 40 to 50 m apart. It was noted that the
                     population was more abundant than had been observed in the last two
                     years.

               Year V - Oysters were collected along the shoreline at the same general locations
                     as previous years. Station 1 was adjacent to the boat ramp. Station 2 was
                     50-m to the south and Station 3 was another 50 m to the south toward the
                     bridge.

               Year  VI - We attempted to sample the same location, but no live oysters of
                     collectable size were available. A few spat in the 1 to 2 cm size range were
                     observed. No samples were collected.

               Year  VII - Oysters were collected along the shoreline in the same general
                     locations as in Year V.

               Year VIII - Oysters were small and extremely hard to find.

               Year IX - This site was not scheduled to be sampled this year, but was used as an
                     alternate for CCIC (Ingleside Cove, Corpus Christi) where there were no
                     oysters to be found. The oysters (at CCNB) were more abundant and easier
                     to find this year.



                                                       73










                   )IMENTCOLLEMONS

               Years I, II, & Iff - Sediments were collected adjacent to each oyster station.
                      Sediments for the NIST archive were also collected adjacent to the oyster
                      stations.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.

               SAMPLING METHOD

                      Oysters - hand
                      Sediment - hand


               WATER DEPM - 0.2 m

               POSSIUBLE CONTAMEINANTS - Contamination factors included the boat ramp,
               marina, Rincon Industrial Park to the west, and Corpus Christi Ship Channel to
               the south.


               ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (IYOO)                (OC)

                    YEAR 1                 27.3                  16.0          29 January 1986
                    YEAR 11                25.6                  11.0          13 January 1987
                    YEAR 111               40.0                  11.0          12 January 1988
                    YEAR IV                38.0                  18.0         15 December 1988
                    YEAR V                 34.6                   9.0         14 December 1989
                    YEAR VII               30.0                  22.0         10 December 1991
                    YEAR VIII              27.7                  11.0         15 December 1992
                    YEAR IX                32.0                  13.0         14 December 1993






















                                                           74














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               SrIE NUMBER - 3                                 DESIGNATOR - CCIC

               SITE - INGLESIDE COVE, CORPUS CHRISTI, TX

               NOXINAL SITE CENTER - 27050.28'N                97014.28W

               LOCATED ON NOS CHART # - 11309

               SITE ACCESS - To reach the site a boat is launched at the public boat ramp at
               Ingleside-On-The-Bay. A 5 minute ride across the La Quinta Ship Channel and
               Ingleside Cove is required to reach the site.

               SITE DESCRIPTION - The site was located along the west side of Ingleside Cove
               channel and north of Ingleside Point on the south tip of the spoil island. Oysters
               were located on an exposed reef and on a mud flat that was exposed during
               normal low tides. The site was surrounded by submerged seagrass beds.
               Landmarks to locate the site were Ingleside Point to the south and the dredge
               spoil island to the north.

               OYS*= COIJXCTIONS

               Year I - The three stations sampled for oysters were located on a tidal reef and on
                     a mud flat. Station 1 was at the center of the reef. Station 2 was from the
                     end nearest the shore, and Station 3 was at the south end of the reef.

               Year II - No oysters were found in Year II and Corps of Engineers surveyors
                     reported that the area had been recently dredged. Oysters were either
                     moved or buried in Year II.

               Year III - There was no significant geographic distinction in the three stations.
                     Oysters were present again during Year III. Most of the oysters present in
                     Year III were very small and appeared to represent a population that had
                     been newly established since the die-off during Year II.            I

               Year IV - In Year IV the stations were the same as those sampled for Year 111.
                     Year IV oysters were small and sparsely scattered.

               Years V, VI, & VII - Sampling sites were in the same locations as previous years
                     with Station 1 in the center of the reef, Station 2 toward the shore 75 in from
                     #1, and Station 3 was 70 m south of #2.

               Year VIII - Only three small oysters were found on this reef, so no samples were
                     taken.

               Year IX - No live oysters were found this year. Oysters were collected from an
                     alternate site - CCNB (Nueces Bay, Corpus Christi).





                                                        '79










                   11MUENT COLLECTIONS

              Years I, II, & III - Each year sediment samples were collected 30 m offshore to
                     the west from each of the oyster collection sites. Submerged seagrasses
                     were present in some of the sediments. Sediment samples only (no oysters)
                     were obtained in Year II at locations sampled in Year I.

              Years IV - No sediments were sampled this year.

              Year V - Sediments were collected adjacent to each of the oysters stations,
                     approximately 15-20 m offshore. Sediments were primarily sand, shell,
                     and grass with sparse areas of mud.

              Years VI, VII, VIII, & IX - No sediments were sampled during these years.

              SAMPLING METHOD

                     Oysters - hand
                     Sediment - hand and box core


              WATER DEPTH - 0.3 m intertidal

              POSSIBLE CONTAMINANTS - Contamination was probable from dredging,
              barge traffic in the La Quinta Channel and from a nearby aluminum plant.

              ENVIRONAUKWAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                 (OC)

                   YEAR 1                  27.0                 23.0           2 February 1986
                   YEAR 11                 28.3                 11.0           13 January 1987
                   YEAR 111                37.0                 10.0           12 January 1988
                   YEAR IV                 35.0                 19.0         14 December 1988
                   YEAR V                  32.7                 13.0         14 December 1989
                   YEAR VI                 35.0                 20.0         17 December 1990
                   YEAR VII                30.0                 20.0         11 December 1991
                   YEAR VIII               31.3                 14.0         15 December 1992
                   YEAR IX                 33.0                 17.0         14 December 1993















                                                           8D
















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                                                Site *3 (CCIC), Corpus Christi Ingleside Cove (from chart 11309).













                                      Xl







                                    -41










               SrIE NUMBER - 53                             DESIGNATOR - CCBH

               SITE - BOAT HARBOR, CORPUS CHRISTI, TX

               NONONAL SITE CENTER - 27050.17'N             97022.72W

               L40CATED ON NOS CHART # - 11309

               SITE ACCESS - This site is located on the northeast end of Rincon Point at the
               junction of Nueces and Corpus Christi Bays. The site is reached by exiting off of
               U.S. Highway 181 at Corpus Christi and driving under the bridge as if going to the
               boat harbor north of the Corpus Christi Ship Channel.

               SITE DESCRIPTION - This collection site was established in Year III. The site
               was located under the bridge (Highway 181). Oysters occurred along the shoreline
               and were attached to concrete and brick rubble. There were both single oysters
               and clumps. The bottom was a mixture of sand, shell, mud, trash, and concrete
               rubble.

               OYSTER COLLECTIONS

               Year III - Station I was located along the shore in shallow water on the northeast
                     end of Rincon Point directly under the Highway 181 bridge. Station 2 was
                     located along the next 100 m section. Station 3 was located farther east
                     along the shoreline adjacent to a trailer park.

               Year IV - The site was not sampled.

               Year V - Sampling stations were located at the same sites originally established
                     in Year III. This site was collected this year as an alternate site, since
                     oysters were unavailable at another site.

               Year VI - There were no live oysters present at the site this year, therefore no
                     collections were made.

               Years VII, VIII, & IX - The site was not sampled during these years.


               SEDDAEM COLLECTIONS

               Year III - Sediment was collected 10 to 20 m offshore of each of the oyster
                     collection stations.

               Years rV, V, VI, VII, VIII, & IX - No sediment samples were collected at this
                     site during these years.





                                                      85










            SAMPLIN'G METHODS

                 Oysters - hand
                 Sediment - hand

            WATER DEPTH - intertidal, 0 - 0.3 m

            POSSIBLE CONTAMINANTS - Potential contamination was present from the
            rubble and trash as well as from boating activities and the Corpus Christi Harbor.

            ENVIFtONM]M1qTAL DATA

                                 Salinity       Temperature     Dates Sampled
                                   (0/00)           (OC)

                YEAR 111           38.0             10.0        13 January 1988
                YEAR V             36.3              8.0       16 December 1989














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                                               Site #53 (CCBH), Corpus Christi Boat Harbor (from chart 11309).





























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               SITE NUMBER - 4                                 DESIGNATOR - ABLR

               SITE - LONG REEF, ARANSAS BAY, TX

               NONEINAL SITE CENTER - 28002.96'N               96056.77V

               LOCATED ON NOS CELART            11314

               SITE ACCESS - The site is located across Aransas Bay almost due east from
               Fulton. To reach the site, launch at the public boat ramp near the harbor. Travel
               across the bay to the ICWW and then turn northeast until channel marker 25 is
               reached. The reef extends ftom St. Joseph Island into the bay.

               SITE DESCRIPTION - During the first four years of the study, all three stations
               were located on the reef which extended from St. Joseph Island toward channel
               marker 25. Oysters were collected by hand from the exposed intertidal reef which
               was composed mainly of dead shell. The site was located at Lat. 28'03.88'N and
               Long. 96157.80'W. for the first four years. In Year V the site was moved due to a
               lack of oysters.

               OYSTERCOLLECTIONS

               Years I & H - Oyster stations were located south of the ICWW. Station 1 was on
                     the first island south of channel marker 25. Bearing to landmarks were
                     255' to white water tower at Rockport and 305' to the middle of the causeway
                     bridge. Station 2 was on the next island to the south at 320' to ICWW
                     marker 25 and 245' to white water tower. Station 2 was located on an island
                     that was a designated bird rookery. Station 3 was on the next island south
                     at 305' to the middle of the causeway bridge and 240* to the white water
                     tower.

               Year  III - Few oysters were present in Year III and no young oysters were
                     observed. Due to the sparse oyster population available by hand, it was
                     recommended that future collections be made by tonging or dredging in
                     deeper water.

               Year IV - The collection stations for Year IV were the same as those of the first
                     three years. Year IV oysters were tonged in approximately 0.5 m water on
                     the west side of the island.

               Year V - Live oysters could not be located at the previously collected locations, so
                     the site was moved along the reef closer to the shore and approximately 1
                     mile to the southeast. Stations were separated by 100 m along the reef

               Years VI, VII, & VIII - The stations used to collect the oysters in Year V was
                     reoccupied and sampled again during these years.

               Year IX - Large oysters were abundant all along the west side of the reef. The
                     collection stations for Year IN were the same as those of the first four years.



                                                         91










                    MRINT COLLECTIONS

               Years I & II - Sediments were collected by box core from the east side of the reef in
                     deeper water in the same order as the oysters.

               Year III - Sediments were collected on the west side of the reef approximately
                     200-m off the reef from each oyster station.

               Year IV - Sediments (NIST) were collected on the west side of the reef
                     approximately 200 m off the reef from each oyster station.

               Years V, VI, VII, VIII, & IX - No sediments were collected these years.

               SAMPLINGMETHOD

                     Oysters - hand and tongs
                     Sediment - box corer

               WATER DEPTH - intertidal, 0 - 0.5 m; subtidal, 0.8 - 2 m, (Year I).

               POSSIBLE CONTAMINANTS - There was possible contamination from marine
               traffic, oil and gas pipelines, and natural contamination from the large numbers
               of birds on the exposed reef.

               ENVIRONMENTAL DATA

                                         Salinity         Temperature         Dates Sampled
                                           (1700)               (0c)

                    YEAR 1                 15.0                 16.0          26 January 1986
                    YEAR 11                15.0                 12.0          13 January 1987
                    YEAR 111               22.0                  9.0          13 January 1988
                    YEAR IV                32.0                 15.0         15 December 1988
                    YEAR V                 32.7                 12.0         15 December 1989
                    YEAR VI                31.0                 18.0         18 December 1990
                    YEAR VII               25.0                 19.0         12 December 1991
                    YEAR VIII              20.0                 15.0         16 December 1992
                    YEAR IEK               23.0                 16.0         16 December 1993
















                                                           92

















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                                                             Site #4 (ABLR), Aransas Bay Long Reef (from chart 11314).












               SrIE NUMBER - 54                               DESIGNATOR - ABHI

               SITE - HARBOR ISLAND, ARANSAS BAY, TX

               NONIINAL SITE CENTER - 27050.33'N              96004.52'W

               LOCATED ON NOS CHART # - 11309,11314

               SITE ACCESS - The collection site established in Year III is located on Mustang
               Island on the south side of the ICWW directly across from Harbor Island. The
               site is at a public access area near fishing piers and is reached by crossing the
               Port Aransas ferry and traveling west past the University of Texas Marine
               Sciences building to the end of the paved road. The site is north of the sand
               parking lot.

               SITE DESCRIPTION - Oysters were attached to rip rap and rubble material
               around the base of the piers. Oysters were generally small and not abundant;
               collections would be best made at low tide since the oysters were growing on
               bottom rubble.


               OYSTER COLLECTIONS

               Year III - Station 1 was located west of the wooden pier. Station I was located 200
                      m to the west. Station 2 was east of the wooden pier. Station 2 was 275 m to
                      west. Station 3 was located 75 m east of Station 2 and west of the three
                      concrete ship docks. Station 3 was 325 m to the west of the wooden pier.

               Years IV, V, VI, VII, VIII, & IX - This site was not sampled during these years.

               SEDIMENT COLLECTIONS

               Year III - Sediment at the oyster site was predominantly sand; therefore, the
                     sediment stations were established 200 m to the west of the oyster stations.
                     Station 1 was a sand and clay bottom 200 m east of Oyster Station 1. Stations
                     2 and 3 were 50 and 100 m farther to the west.

               Years IV, V, VI, VII, VIII, & IX - This site was not sampled during these years.

               SAMPLING METHODS

                     Oysters - hand
                     Sediment - hand

               WATER DEPTH - intertidal, 0 - 0.4 m

               POSSIBLE CONTAMINANTS - Numerous potential sources of contamination
               existed at this site. Since it was located along the Corpus Christi Ship Channel, it
               received effluents from passing ships as well as non-point pollution from the
               nearby harbor at Port Aransas and industrial waste from Harbor Island.



                                                       97




                                                                                                  I
             ENVIRONMENTAL DATA                                                                   I

                                    Salinity       Temperature      Dates Sampled
                                     (%o)              (.C)                                       I
                 YEAR 111            30.0              10.0         13 January 1988               1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
                                                                                                  1
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              SITE NUMBER - 5                               DESIGNATOR - CBCR

              SITE - COPANO REEF, COPANO BAY, TX

              NOMINAL SITE CENTER - 28008.47'N               97007.67'W


              1A)CATED ON NOS CHART # - 11314

              SITE ACCESS - The site is located on the north side of Copano Bay at Copano
              Point. A boat must be used to reach the site and is launched at Pouzee's Marina,
              at Lone Tree Point, Rockport. From Pouzze's, go northwest to Copano Reef, an
              exposed intertidal reef.

              SITE DESCRIPTION - The reef ran southeast from Copano Point and was located
              around the white pole with Texas Parks and Wildlife marker "Copano Reef."
              This reef has been on the decline for the past five years. Oysters are small and
              sparse.

              OYSTER COLLECTIONS

              Years I & II - Station 1 was 100 m north of the Texas Parks and Wildlife marker,
                     closest to the shoreline. Station 2 was adjacent to the marker. Station 3 was
                     100 m south of the marker. In the first two years of the project, there were
                     abundant oysters which were collected by hand.

              Year III - There were few live oysters due to prolonged freshwater conditions
                     during the spring. Numerous oysters were present which had died during
                     the past season, and no young oysters or spat were observed at the site in
                     Year III.

              Year IV - In Year IV, oysters were collected at the same locations as sampled the
                     previous three years. Year IV oysters were very small and difficult to
                     obtain.

              Year   V & VI - Oysters were collected along the same segment of the reef as
                     previous years. Oysters were abundant this year.

              Year   VII - Oysters were collected along the same segment of the reef as in
                     previous years. Oysters were not abundant. Most were very small and
                     many were dead.

              Year VIII - The reef was dead, so no oysters were collected.

              Year LY - No live oysters were found on the reef this year. Lots of small mussels
                     were found to be growing on all the dead oyster shells. MBAR (Ayres Reef,
                     Mesquite Bay) was used as an alternate site for oyster sampling.





                                                      103




		SEDIMENT COLLECTIONS

		Years I,II & III - Sediments were collected in subtidal areas in 1 to 1.5 m water
			and approximately 50-100 m west of the respective oyster sample area.

		Years IV & VI - No sediments were sampled during these years.

		Year V - Sediments for Station 1 were collected 50 m west of Oyster Station 1 in 1.5
			m of water.  Station 2 sediments were located 60 m west of the oysters.
			Station 3 sediments were 50 m west of station 3 in 1.5 m of water.

		Years VII, VIII, & IX - No sediments were collected during these years.

		SAMPLING METHOD

			Oysters - hard
			Sediment - boxcore

		WATER DEPTH - intertidal, 0 to 0.5

		POSSIBLE CONTAMINANTS - There were no observable sources of
		contamination.

		ENVIRONMENTAL DATA

					Salinity		Temperature		Dates Sampled
					   %				C

		YEAR I		16.2			   17.0		25 January 1986
		YEAR II		14.0			   17.0		14 January 1987
		YEAR III		20.0			    9.0		13 January 1988
		YEAR IV		29.0			   15.0		15 December 1988
		YEAR V		35.0			   10.0		13 December 1989
		YEAR VI		26.0			   21.0		18 December 1990
		YEAR VII		14.0			   19.0		11 December 1991
		YEAR VIII		14.0			   15.0		16 December 1992
		YEAR IX		16.0			   18.0		15 December 1993


									104
			

















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               SHENUMBER-6                                   DESIGNATOR - M13AR

               SITE - AYRES REEF, MESQUITE BAY, TX

               NONONAL SITE CENTER - 28010.15'N              96049.95V

               IOCATED ON NOS CELART # - 11314

               SITE ACCESS - The boat was launched at Fulton Beach, Texas, at the Sandollar
               Pavilion during Years I and II and the ICWW was followed northeast to channel
               marker 79. In Year III, the boat was launched at Goose Island State Park and in
               Years IV and V the boat was launched at Port O'Connor. Regardless of where
               the boat is launched, run time to the site is at least an hour in good weather.

               SITE DESCRIPTION - This site was located on the north side of Mesquite Bay
               between Matagorda Island to the southeast and the Aransas National Wildlife
               Refuge to the north. The reef was oriented in a northwest-southeast direction and
               the site was located in the middle of the reef at Ayres Dugout.

               OYSTER COLIECTIONS

               Years 1, 11, 111, IV, V, &VI - Oysters and sediments were collected by hand on
                     the intertidal reef at the same stations each year. During some years, the
                     reef silted over but oysters were still present. Station 1 was on the east end
                     of the Dugout Cut. Station 2 was in the middle of the Dugout. Station 3 was
                     on the west portion of the Dugout. There was an abundant supply of large
                     oysters.

               Year  VII - Oysters were collected at the same stations described for previous
                     years. This year oysters were mostly dead and there was little indication of
                     reproduction.

               Year  VIII - No live oysters were found at last year's location, so the site was
                     moved -0.25 miles to the south. The site is located on a small brushy island
                     with a red reflector on a post. Site coordinates (GPS) for this year's
                     collection are Lat. 28' 09.82N and Long. 96' 49.96W.

               Year IX - This site was not scheduled to be collected in 1993, but was used as an
                     alternate to CBCR (Copano Reef, Copano Bay). The oysters were larger and
                     more abundant, and were collected from the same stations as in Year VIII.

               SEDD4ENTCOLLECTIONS

               Years I, II, & III - Sediments were collected approximately 400 m to the northeast
                     of the Dugout in Ayres Bay.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                     years.





                                                       109










               SAMPLING METHOD

                      Oysters - hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0 to 1.0 M

               POSSIBLE CONTAMINANTS - No observable sources of contamination were
               present.

               ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                            (%0)                (0c)

                    YEAR 1                  16.0                13.0           28 January 1986
                    YEAR 11                 10.6                12.0           13 January 1987
                    YEAR 111                27.0                  8.0          14 January 1988
                    YEAR IV                 24.0                15.0          15 December 1988
                    YEAR V                  29.0                13.0          15 December 1989
                    YEAR VI                 29.0                22.0          17 December 1990
                    YEAR VII                24.0                21.0          12 December 1991
                    YEAR VIII               20.0                15.0          16 December 1992
                    YEAR ICK                24.0                13.0          15 December 1993

































                                                           110














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                                                                                  Site #6 (MBAR), Mesquite Bay Ayres Reef.











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               SITE NUMBER - 7                                DESIGNATOR - SAPP

               SITE - PANTHER PT. REEF, SAN ANTONIO BAY, TX

               NOM[INAL SITE CENTER - 28014.00'N              96042.55V

               LOCATED ON NOS CHART # - 11315

               SITE ACCESS - To reach the site a boat is launched at Seadrift (Haynies Seafood
               or the Public Ramp at Swan Point), Port O'Connor (Doc's Doc) or Port Lavaca.
               Run time by boat from Seadrift is 30 min, 2 to 3 hours from Port Lavaca, and 1
               hour from Port O'Connor. Do not use the Swan Point ramp at low tide - as the
               area is very shallow. The site is located on the reef that extends to the northwest
               off of Panther Point.

               SITE DESCRIPTION - This submerged reef was located 300 to 400 ni from a
               production platform at a bearing of 2600 and 100 m from the black and white PVC
               pole marking the top of the reef. This subtidal reef was located to the northeast
               and east side of the white plastic pole off the northwest end of Panther Point.
               Oysters and moderately dense "grass beds" were widely dispersed on the sandy
               bottom.


               OYSTER COLLECTIONS

               Years I & II - Samples during Years I and 11 were collected by dredge but could
                     be collected by tongs in calm weather. Oysters were numerous in the first
                     two years. Station distinctions were not made.

               Year  III - There were no oysters present at this site or any of the reefs in the
                     nearby areas. Local and state biologists reported that all of the reefs were
                     dead due to prolonged freshwater conditions during the spring. The
                     submerged seagrasses and seaweeds normally present were absent.

               Year  IV - As in Year III, there were no collectible oysters present due to the
                     massive die-off in the San Antonio and Lavaca Bays in the spring of 1987.
                     Spat in the size range of 0.5 to 0.75" were observed but were not numerous.
                     In the absence of another major physical stress, there will probably be
                     oysters next year.

               Year  V - Station 1 was on the submerged reef on a bearing of 280' to the
                     production platform. The bottom was shell with sandy silt. Oysters were
                     tonged. Station 2 was 100 m to the southeast (bearing 150") between the two
                     white poles on the west side of the reef. The site was subtidal but the oysters
                     were collected by hand in water only 0.2 ni deep. Station 3 was another 200
                     m south (bearing 150'). Oysters were tonged at this location. Oysters at all
                     three stations were mixed with abundant shell and marine algae.





                                                       115









               Year  VI - Oysters from all three stations were collected by hand at Year V's
                      Station 2. All stations were collected in one location due to broken oyster
                      tongs.

               Year VH - Oysters were       collected with a dredge from the top of the submerged
                      reef. Collections were made at the same locations described for Year V.
                      Station 1 was on the North end of the reef. Station 2 was on the midsection
                      in shallow water. Station 3 was on the south end of the reef .

               Year  VIII - Oysters were very scarce at this reef. All the oysters were collected
                      along the reef top near station 2. Stations were not distinguished due to the
                      small number of oysters present.

               Year IX - Oysters were abundant again this year and easily collected. The
                      samples were dredged from the same stations as those in Year V.

                   )I[MENT COLLEMONS

               Years I, H, & III - Sediments were collected at the same stations where oysters
                      were located.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.

               SAMPLING M[ETHOD

                      Oysters - dredge, tongs, and hand
                      Sediment - box core

               WATER DEPTH - subtidal, 0.2 to 1.5 m.

               POSSIBLE CONTAMEENANTS - A nearby possible source of contamination was an
               oil or gas platform bearing 2600 from the reef, 0.25 to 0.5 mile distant.

               ENVIRONM[ENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (700)                (OC)

                    YEAR 1                  18.6                 18.0           February 1986
                    YEAR H                    5.0                12.0          15 January 1987
                    'YEAR 111               30.0                   8.0         13 January 1988
                    YEAR IV                 21.0                 12.0          15 December 1988
                    YEAR V                  24.4                 10.0          15 December 1989
                    YEAR VI                 26.0                 19.0          17 December 1990
                    YEAR VII                21.3                 22.0          11 December 1991
                    YEAR VIII               20.0                 14.0          16 December 1992
                    YEAR IN                 24.0                 15.0          15 December 1993





                                                           116


















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               SHE NUAE3ER - 8                                DESIGNATOR - SAMP

               SITE - MOSQUITO POINT, SAN ANTONIO BAY, TX

               NONONAL SITE CENTER - 28020.65'N               96042.78V

               WCATED ON NOS CHART # - 11315

               SITE ACCESS - Access is by boat launched at Seadrift (Haynies Seafood or the
               Public Ramp at Swan Point), Port O'Connor or Port Lavaca. Run time by boat
               from Seadrift is 30 min, 2 to 3 hours from Port Lavaca, or 1 hour from Port
               O'Connor. From Swan Point, near Seadrift, exit the channel which is very
               shallow at low tide and proceed southeast in the Victoria Barge Canal to channel
               marker 15. At marker 15, turn southwest to the exposed reef There are no
               landmarks on shore for reference.

               SITE DESCRUITION - The site was located off Mosquito Point west of the Victoria
               Barge Canal and southwest of channel marker 15. Oysters were collected on the
               exposed intertidal reef. A six legged wooden platform was located 80 m to the
               southeast of the reef.

               OYSTERCOLLECTIONS

               Years I & II - Oysters were abundant at this location during Years I and II.
                     Station I was at the midpoint of the exposed reef, Station 2 was 150 yds south
                     on the west side, and Station 3 was another 200 yds south of Station 2. In
                     Year II, the station order was reversed with Station 1 on the west end of the
                     reef, Station 2 was 200 ft to the east, and Station 3 was another 200 ft east.

               Year III - In Year III, there were no live oysters present on the reef and no spat
                     or young oysters were observed.

               Year IV - As in Year III, there were no live oysters present due to the massive
                     die-off in the San Antonio and Lavaca Bays in the spring of 1987.

               Year V - Still no oysters alive on this reef. No samples were collected this year.

               Year  VI - Oysters were again present. Collections were made on the reef
                     southeast of Mosquito Point. Station 1 was on the north end of the reef,
                     Station 2 was on the middle part of the exposed reef, and Station 3 was on
                     the southwest end of the reef.

               Year  VII - Oysters were collected at the same locations described last year.
                     Oysters were much more abundant this year.

               Year  VIII - The reef was dead; therefore no oyster samples were available this
                     year.

               Year IX - The site was not sampled this year.



                                                        121










                   )DIEW COLUCTIONS

               Years I and II - Sediments were collected in subtidal areas adjacent to the oyster
                      stations from mud and sand bottom in Years I and II. Station 1 was on the
                      west side 10-50 feet out. Station 2 was 50 yards south of Station 1 (intertidal)
                      and 200 yards south was Station 3.

               Year III - Sediment samples were collected from soft sandy mud bottom over shell
                      hash approximately 100 to 200 m north of the reef in 1 to 1.5 m of water.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.

               SAMPLING METHOD

                      Oysters - hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0 - 0.5 m

               POSSEBLE CONTAAIINANTS - No contaminants were observed but the potential
               for contamination existed since the site was located within a half mile of the
               Victoria Barge Canal.

               ENVIRONADEWAL DATA

                                         Salinity          Temperature         Dates Sampled
                                            (%0)                 (OC)

                    YEAR 1                  14.0                 20.0          2 February 1986
                    YEAR II                  -                   12.0          14 January 1987
                    YEAR 111                21.0                  8.0          14 January 1988
                    YEAR IV                 23.0                 16.0         14 December 1988
                    YEAR V                  24.3                 10.0         15 December 1989
                    YEAR VI                 25.0                 19.0         18 December 1990
                    YEAR VII                18.0                 20.0         11 December 1991
                    YEAR VIII               15.0                 14.0         16 December 1992

















                                                           122
















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               SrM NUMER - 9                                 DESIGNATOR - ESSP

               SITE - SOUTH PASS REEF, ESPIRITU SANTO, lix

               NOMINAL SITE CENTER - 28017.90'N               96037.33V

               LOCATED ON NOS CHART # - 11315) 11319

               SITE ACCESS - The site is accessed by boat launched at Swan Point, near
               Seadrift. Proceed southeast on the Victoria Barge Canal to the ICWW and then
               east to the site. Alternate launch points are Snapper Snatcher dock or Doc's Doe
               in Port O'Connor. South Pass Island, a few hundred meters to the southeast of
               the site, is an oil production site and has a compressor station and buildings.
               Approximate time to the site is 0.75 hrs.

               SITE DESCRIPTION - The site was located between Grass Island on the north
               and Steamboat Island on the south. The site was composed of a series of low
               exposed reefs at 105* from pumping station, 240' from water tower and 150' from
                outheastern most point of Steamboat Island. The sample area was an intertidal
               reef.
               S



               OYSTERCOLLECTIONS

               Years I & II - Oyster collection areas were 100 m apart on a north-south line
                     along the exposed reef. Oysters were abundant during both years.

               Year III - In Year III, there were no oysters present. Texas Parks and Wildlife
                     biologists reported that the reefs in Espiritu Santo Bay had died due to
                     excessive freshwater inflow during the spring and summer periods.

               Year X - As in Year III, there were no collectible oysters present in Year IV due
                     to the massive die-off in the San Antonio and Lavaca Bays in the spring of
                     1987. Spat were observed but were not numerous. In the absence of another
                     physical stress, there will probably be oysters next year.

               Years V & VI - Oyster were present in adequate numbers. Station 1 was on the
                     reef which was northwest of the tanks and west of the island where station
                     3 was located. The three tanks at the production facility were located on a
                     bearing of 130'. Station 2 was north of the production station, to the east of
                     Station 1 on the same island. It was 180' to the channel marker pole and
                     the top of the small island was to the west. Station 3 was northeast of the
                     tanks on the end of the island with the top always exposed and in line with
                     the island with the Yuccas. There were abundant shell, shell hash, and
                     small oysters with attached marine algae.

               Year VII - Collection sites were in the same locations as the last two years.

               Year VIII - The reef was dead; therefore, no oysters were collected.








              Year IX - Oysters were present again this year, and collected from the same
                     stations as used in Years V through VII.

              SEDIMENT COLLECTIONS

              Years I, A & III - Sediments were taken from subtidal mud 500-1000 m to the
                     north at three random stations in all years. Sediments were loose gray
                     muds overlain with loose brown mud.

              Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                     years.

              SAMPLING METHOD

                     Oysters - hand
                     Sediment - box corer

              WATER DEPM - intertidal, 0.1 - 0.5 m.

              POSSIBLE CONTAMINANTS - Contamination factors included oil and gas
              pipelines and marine traffic.

              ENVIRONMENTAL DATA

                                         Salinity         Temperature         Dates Sampled
                                           (1700)               (OC)

                   YEAR 1                  22.0                 17.0          1 February 1986
                   YEAR 11                 9.1                  12.0          15 January 1987
                   YEAR 111                37.0                 10.0          13 January 1988
                   YEAR IV                 30.0                 15.0         15 December 1988
                   YEAR V                  30.0                 11.0         15 December 1989
                   YEAR VI                 30.0                 21.5         17 December 1990
                   YEAR VII                27.6                 21.0         11 December 1991
                   YEAR VIII                -                   14.0         16 December 1992
                   YEAR IX                 2Z.0                 17.0         16 December 1993











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                SrIE NUMBER - 10                               DESIGNATOR - ESBD

                SITE - BILL DAYS REEF, ESPIRITU SANTO, TX

                NONDNAL SITE CENTER - 28024.85'N               96026.27V

                WCATED ON NOS CHART # - 11315,11319

                SITE ACCESS - Access to the site is from Port O'Connor via the ICWW to Espiritu
                Santo Bay.

                SITE DESCRIPTION - The site was located at Bill Days Reef which was in
                Barroom Bay south of Port O'Connor. The reef was oriented almost east-west.
                During the first two years of the study there were no live oysters.

                OYSTER COLLECTIONS

                Years I & II - The site was visited both in 1986 and 1987 and no living oysters were
                      found.

                Year III - During Year III, live oysters were found. Oysters were collected by
                      hand on the exposed portion of the south side of Bill Days Reef in intertidal
                      water near the northernmost duck blind at the east end of the reef No
                      distinct stations were established. Oysters were very small but were
                      numerous. Abundant spat were present.

                Year  IV - Oysters were again present with small oysters in clumps on dead
                      shells. In Year IV, stations were established. Station 1 was on the west
                      end of the reef at bearing of 360' to Port O'Connor water tower; 340' to
                      Milchem tanks; 250 m south of the reef; Station 2 was 100 m to the east in
                      the center of the reef on a line with the west end of Bill Days Reef and the
                      tall tower to the west; and Station 3 was 50 m to the east.

                Year  V - Stations which were established in Year IV were sampled again.
                      Oysters were abundant but small.

                Year VI - No oysters were present in the required size range but a few 1 to 2 cm.
                      oysters were present. Collections were not made at this site.

                Years VII & VIII - Oysters were present in adequate numbers for sampling, but
                      were not very abundant.

                Year IX - Oysters were abundant this year, and collected from the same stations
                      as in Years VII & VIII.


                SEDIM]MW COLLECTIONS

                Years 1, 11, & III - Sediments were composed of loose gray silt over gray sandy
                      clay and were collected adjacent to the reef each year.










             Years X, V, VI, VII, VIII, & IX - No sediments were sampled during these
                    years.

             &AWLMG METHOD

                    Oysters - hand
                    Sediment - hand

             WATER DEPTH - intertidal, 0.3 m

             POSSIBLE CONTAMINANTS                   No observable sources of contamination were
             found.


             ENVIRONMENTAL DATA

                                        Salinity          Temperature         Dates Sampled
                                          (9010)               (0c)

                  YEAR 1                  28.0                 21.0           1 February 1986
                  YEAR 11                 25.6                 15.0           15 January 1987
                  YEAR 111                36.0                   8.0          14 January 1988
                  YEAR IV                 34.0                 16.0         14 December 1988
                  YEAR V                  34.0                 14.0         16 December 1989
                  YEAR VI                   -                  18.0         19 December 1990
                  YEAR VII                30.0                 20.0         11 December 1991
                  YEAR VIII               30.0                 16.0         17 December 1992
                  YEAR IX                 30.0                 16.0         16 December 1993




























                                                         134

















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               SITE NUMBER - 11                               DESIGNATOR - MBLR

               SITE - LAVACA RIVER MOUTH, MATAGORDA BAY TX

               NOMINAL SITE CENTER - 28039.80'N               96034.83V

               LOCATED ON NOS CHART # - 11317

               SITE ACCESS - This site was originally accessed by launching the boat at Port
               Lavaca State Park and proceeding for 0.5 hour to the mouth of the Lavaca River.
               The site was moved in Year IV. The new site is accessible from Highway 35 and
               can be sampled entirely by hand.

               SITE DESCRIPTION - The original site was a submerged reef approximately 0.5
               mile west of the river mouth. The highway 35 bridge was to the south and the
               plastics plant to the east. The site was an exposed reef on the east side of the
               channel entering the Lavaca River. Oysters were located only on the west side of
               the reef between channel marker 22 and the river mouth to the north. Most of the
               reef was dead and oysters were sparse. The site was originally located at Lat.
               28'41.00'N and Long. 96'34.65W. In Year IV, this site was moved because of the
               difficulty in finding oysters in Year III and the absence of oysters in Year IV at
               the original location. The site was moved to the north shore of the causeway
               where it joins the Highway 35 bridge.

               OYSTER COLLECTIONS

               Year I - In Year I the oysters were collected by tonging from a subtidal reef west
                     of the river mouth. Station bearings were: Station 1 was 185' to the middle
                     span of bridge; 90' to the water tower at plastics plant. Station 2 was 85' to
                     the water tower at plastics plant; 195' to the middle span of the bridge.
                     Station 2 is 0.25 mile south of Station 1. Station 3 was 230' to the middle
                     span of the bridge; 70' to the water tower at the plastics plant. Station 3 was
                     0.25 mile south of Station 2.

               Year  II - No distinctions were made for Stations 1, 2, and 3 during Year II.
                     Oysters were collected by hand. The reef could not be relocated in Year II;
                     therefore, there was a slight difference in site location by approximately 500
                     to 1000 m between Years I and II.

               Year III - No oysters were present in Year III. Conversations with Texas Parks
                     and Wildlife biologists indicated that the reef die-off was due to prolonged
                     freshwater inflow.

               Year IV - Station I was located at the end of the causeway. Station 2 was located
                     approximately 300 m east of Station 1, where a spit/reef extended into the
                     bay from the shoreline. Station 3 was approximately 400 m east of Station
                     2. Commercial oystering was observed just north of the bridge, between the
                     center span and the power lines to the north.








               Years V, VI, VII, & VIII - Oysters were collected in the same locations as
                      described for Year IV. Oysters were numerous each year.

               Year ff - The site was not visited this year.

                   MUNT COLLEMONS

               Years I, II, & III - Sediments were collected with a boxcorer at stations spaced
                      equidistant and extending from channel marker 22 to the river mouth.
                      Sediments for the NIST archive were collocated with the oysters.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.

               SAMPUNGMETHOD

                      Oysters - dredge, tong or hand
                      Sediment - box core

               WATER DEPTH - 0.2 - 2 m, subtidal (Years I & II), intertidal (subsequent years)

               POSSIBLE CONTAMINANTS - Possible contamination could be from the plastics
               plant to the southeast of the site and the Alcoa plant some distance to the south in
               Lavaca Bay.

               ENVEFtONAIENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           MO)                  (0c)

                    YEAR 1                 17.3                 17.0          31 January 1986
                    YEARII                   8.0                  9.0         13 January 1987
                    'YEAR 111              23.0                   8.0          12 January 1988
                    YEAR IV                35.0                 18.0          14 December 1988
                    YEAR V                 29.0                 11.0          15 December 1989
                    YEAR VI                30.0                 20.0          18 December 1990
                    YEAR VII               23.0                 23.0          13 December 1991
                    YEAR VIII              15.0                 16.6          16 December 1992
















                                                          140


















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                                                                              Site #11 (MBLR), Matagorda Bay Lavaca River Mouth (from chart 11317).











              SM NUMER - 12                                  DESIGNATOR - MBGP

              SITE - GALLINIPPER PT., MATAGORDA BAY, TX

              NON[INAL SITE CENTER - 28035.25'N              96034.17W

              LOCATEI) ON NOS CHART # - 11317

              SITE ACCESS - The boat is launched at Port Lavaca State Park and the channel is
              followed down to Gallinipper Point at channel marker 63. An alternate closer
              launch site is in the Refuge Harbour, with a marked channel leading out to
              Gallinipper Point.

              SITE DESCRJIPTION - This site was a submerged reef approximately 400 m off the
              north shore of Gallinipper Point. The reef ran on a1ine from the aluminum plant
              to a house on the shoreline that had an asymmetrically pitched roof and a
              fireplace chimney. The reef was south of channel marker 63 and was
              approximately 400 m offshore.

              OYSTER COL1,ECTIONS

              Years I & II - In Years I and II, the stations were distributed on the reef line at
                     distances of 200, 300, and 400 m from shore. Station bearings were: Station
                     1 - 360' to the orange/white range tower; 275' to the twin radio towers.
                     Station 1 - 50 m from the high range marker. Station 2 was 325' to tower
                     with range light; 290' to the twin radio-towers. Station 2 was 400 m from
                     the shore. Station 3 - 30' to tower with range light; 285' to the twin radio
                     towers. Station 3 was at the eastern end of Alamo Beach. Oysters were
                     dredged using a commercial boat.

              Year III - In Year III the oysters were purchased from commercial fishermen
                     with Stations 1 and 2 located in approximately the same areas but Station 3
                     was a mix of Stations 1 and 2 oysters. The reef supported many live, good
                     sized oysters. It was not fished commercially or privately during the first
                     two years, since it was rumored to be a closed area due to pollution. There
                     were numerous oyster boats dredging in Year III.

              Year   IV - In Year IV, the same three sites were dredged as were sampled in
                     Year II. Oysters were dredged by a commercial oysterman at our request
                     and direction.

              Year V - The same areas and numerous adjacent sites were examined for oysters
                     but none could be located; therefore, an alternate site, which had previously
                     been sampled, was selected in another bay system.

              Year VI - Oysters were present again this year. Oysters were collected with a
                     clam dredge. Station 1 was 100 to 200 m from the shoreline on a line
                     between the geodesic dome house and the range marker. Station 2 was 150



                                                      145








                        to 200 m to the north along the same line. Station 3 was another 100 m to
                        the north, directly south of the range marker.

                Years VII & VIII - Oysters were present these years and collections were made
                        in the same location as described for Year VI.

                Year IX - Oysters were not very common this year, with an abundance of fresh
                        dead oyster shells being present on the site. The samples were collected in
                        a line between the Geodesic dome house and the range marker; Station 1
                        being 100m south of the range marker, Station 2 being the midpoint, and
                        Station 3 being 150m offshore from the house.

                    3IME,M COLLECTIONS

                Years I, H, & III - Sediments in Years I, II and III were obtained by boxcore
                        from an area northwest of the reef between the high range marker and
                        Alamo Beach.

                Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                        years.

                SAMPLINGMETHOD

                        Oysters - The site required dredging on a completely subtidal reef
                        Sediment - box core

                WATER DEPITI - subtidal, 2 m

                POSSEBLE CONTAMINANTS - Possible point contamination sources were the
                Alcoa aluminum plant directly across the bay, and the plastics plant near the
                Lavaca River mouth.


                ENVIRONADMAL DATA

                                             Salinity           Temperature           Dates Sampled
                                               0700)                  (0c)

                      YEAR 1                   23.0                   17.0            31 January 1986
                      YEARII                   16.0                     9.0           13 January 1987
                      YEAR 111                 29.0                     7.0           12 January 1988
                      YEAR IV                  36.0                   13.0           14 December 1988
                      YEAR V                     -                     -                not sampled
                      YEAR VI                  31.0                   19.0           18 December 1990
                      YEAR VII                 24.3                   20.3           12 December 1991
                      YEAR VIII                20.0                   13.0           17 December 1992
                      YEAR IEK                 25.0                   15.0           14 December 1993






                                                                146























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               SITE NUMBER - 13                               DESIGNATOR - MBTP

               SITE - TRES PALACIOS BAY, MATAGORDA BAY TX

               NONEENAL SITE CENTER - 28039.50'N              96013.45'W


               WCATED ON NOS CELART # - 11317

               SITE ACCESS - In Years I and II, the boat was launched at Port Lavaca State
               Park. The transit across Lavaca, Matagorda, and Palacios Bays takes 1.5 hours.
               In Year III and subsequently, the boat was launched at the public boat ramp at
               Palacios and then proceeded southwest out the Palacios Channel for two nautical
               miles to channel marker 48 and then south-southeast across the bay to the
               northeast end of the Coon Island Reef (15 minute run time).

               SITE DESCRIPTION - The site was located on a string of reefs across the mouth
               of Green Island Bay. The reef extends from Coon Island to Oliver Point. Station 1
               at the north end of the reefs was closest to Palacios.


               OYSTER COLLECTIONS

               Years I, H, III, IV, V - Oyster collection stations remained the same during. each
                     year of sampling. Station 1 was on the north side of Coon Island (the
                     northernmost of three exposed islands/reefs running from Oliver Point to
                     the northeast). Station 2 was taken from the westward end of the middle
                     island, between Stations 1 and 3, and is due east of Red Chi #2. Station 3
                     was closest to Coon Island at the westernmost reef/island (samples from
                     the north side and east end of this island). Station 3 was on a bearing of 150
                     degrees to the platform.

               Year VI - Sample sites remained the same as in previous years. A new channel
                     marker (red 2) had been installed which provided an additional reference
                     point.

               Years VII & VIII - Oyster collection stations were the same as the previous seven
                     years. Oysters were not as abundant this year.

               Year IX - The site was not visited this year.

               SEDIMENT COLLECTIONS

               Year I - Sediments were collected by hand in close proximity to the oyster sites.

               Years II & III - Sediments were collected by boxcorer at stations located 150-
                     200-m, southwest from the oyster stations.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                     years.





                                                       151










               SAMPLINGMETHOD

                      Oysters - tong and hand
                      Sediment - hand and box corer

               WATER DEPTH - intertidal, 0 to 1 meter

               POSSIBLE CONTAMINANTS - There were no obvious sources of contamination
               in the vicinity except for a couple of oil or gas production platforms. Natural
               contamination may have been present since the entire middle island smelled of
               bird droppings and the waters surrounding the reef had abundant brown and
               green algae.

               ENVIRON'MENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (%0)                 (0c)

                    YEAR     1              24.0                 21.0           1 February 1986
                    YEAR     11             20.0                 14.0           14 January 1987
                    YEAR     111            35.0                   7.0          12 January 1988
                    YEAR     IV             34.0                 14.0          13 December 1988
                    YEAR V                  26.0                 14.0          14 December 1989
                    YEAR VI                 30.3                 22.3          16 December 1990
                    YEAR VII                22.0                 21.0          10 December 1991
                    YEAR     VIII           22.0                 15.0          15 December 1992































                                                           152























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                                                                               Site #13 (MBTP), Matagorda Bay Tres Palacios Bay.













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               SITE NUMBER - 14                               DESIGNATOR - MBEM

               SITE - EAST MATAGORDA, MATAGORDA BAY, TX

               NONONAL SITE CENTER - 28042.67'N               95053.OOrW

               LOCATED ON NOS CHART # - 11317

               SITE ACCESS - The site is accessed via boat launched in Matagorda at C&R
               Marina and Bait Camp next to the Matagorda Drawbridge. Proceed east along
               the ICWW to the first cut to the south. Collection areas are -100 m into East
               Matagorda Bay at the reefs located on either side of the channel.

               SITE DESCRIPTION - The exposed reefs extended out from Old Gulf Cut into East
               Matagorda Bay. Oysters and sediments were collected from mainly dead reefs
               which had silted over. There were very few live oysters, and all were very small
               during the first four years of study.

               OYSTER COLLECTIONS

               Years I, II, III, IV, V, & VI - Oysters were collected at the same locations during
                     all years of the study. Station 1 was directly outside the cut, with a bearing
                     of 350 to microwave tower on the ICWW and 1060 to a group of six pilings in
                     the bay. Station 2 is 200 m south of Station 1, with bearing of 24' to
                     microwave tower; 95' to group of six pilings. Station 3 is on the third
                     exposed reef west of the Old Gulf Cut and was 250 m west of Station 1.
                     Landmarks were 40' to the microwave tower and 80' to a group of six
                     pilings.

               Years VII &VIII - Oyster collection stations were at the same locations described
                     above.

               Year LY - The site was not scheduled for sampling this year.

               SEDIMENT COLLECTIONS

               Years I, II, & III - Sediment was collected 50 m west of each of the three oyster
                     stations.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                     years.

               SAMPLING METHOD

                     Oysters - hand
                     Sediment - box core

               WATER DEPTH - intertidal, 0 to 0.5 m



                                                       157









               POSSIBLE CONTAMINANTS - No observable sources of contamination were
               evident except for silting over of the reef.

               ENVERONADENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (700)                (0c)

                    YEAR 1                 22.6                 18.0           30 January 1986
                    YEAR 11                  8.6                11.0           12 January 1987
                    YEAR 111               30.0                   6.0          11 January 1988
                    YEAR IV                36.0                 15.0          13 December 1988
                    YEAR V                 24.7                 10.0          14 December 1989
                    YEAR VI                30.0                 22.0          16 December 1990
                    YEAR VII               20.0                 20.0          10 December 1991
                    YEAR VIII              22.3                 13.0          15 December 1992










































                                                           158




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                                      Site #14 (MBEM), Matagorda Bay East Matagorda (from chart 11319).


























































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               SrIE NUMBER 56                                 DESIGNATOR - MBCB

               SITE - CARANCAHUA BAY, MATAGORDA BAY, TX

               NOMINAL SHE CENTER - 28039.40'N                96023.18W

               LOCATED ON NOS CELART # - 11318

               SITE ACCESS - This collection site, which was established in Year III, is
               accessed via boat from a launch point on Highway 35. The public boat ramp
               located on the highway is silted in near the mouth so that only a small boat can be
               launched. Proceed directly south down the bay for 4.5 nautical miles and then
               turn to the east toward a point with a PVC marker. The site can also be reached
               by land from Highway 35, by turning south at the intersection of Highway 35 &
               1862, onto paved Jackson County Road 476. Then turn right onto County Road 477,
               and south again to Bay Avenue. Turn right onto Bay Avenue and go to the end of
               the road. The site is located approximately 100 to the west.

               SITE DESCRIPTION - The site was located on the east side of Carancahua Bay
               directly across the bay from Port Alto. Several houses were located on or near this
               point. The site was a shoreline site and could be collected from the shore at low
               tide. Oysters were very abundant at Stations 2 and 3 but were less so at Station 1.
               No live oysters were found in the upper part of the bay, presumably due to
               prolonged freshwater conditions which were common during the spring of 1987.

               OYSTERCOLLECTIONS,

               Year III - Oysters were collected at this site for the first time in Year III. Station
                     I was located approximately 300 m south of a green house along the
                     shoreline. Station 1 was 10 to 20 m off the shoreline on a small reef
                     paralleling the shore for approximately 80 m, oysters were found on the
                     bottom or attached to shell. Station 2 was on the point which extended to the
                     southwest. At this site the oysters were attached to concrete and brick
                     rubble. The station extended approximately 100 m into the bay. Station 3
                     was located southeast of Station 2 on an intertidal reef along the shore and
                     on an exposed reef approximately 20 m from the shore.

               Year IV - Oysters were not sampled during this year.

               Year V - This site was used as a substitute for MBGP. Oysters were abundant
                     and were collected in the locations that were sampled during Year III.

               Years VI & VII - This site was not sampled during these years.

               Year VIII - This site was used as a substitute for dead reefs futher south. Oysters
                     were abundant and were collected at the locations sampled during Year V.





                                                        163









               Year IX - Oysters were sampled from the same station locations as in Year III,
                      with Stations 2 and 3 being interchanged. Large oysters were abundant
                      througthout the area and were easily collected by hand at low tide.

                   31MENT COLLEMONS


               Year III - Sediment stations were located 200 m to the south and west of each
                      oyster station.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.

               SAMPUNG M[ETHODS

                      Oysters - hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0 to 0.3 m

               POSSEBLE CONTANEINANTS - No sources of contamination were obvious within
               the bay; however, the inflow and runoff into Carancahua Bay was from the
               adjacent agricultural lands which were a potential non-point source for pesticides
               and agricultural chemicals.

               ENVIRONMENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (9010)               (OC)

                    YEAR 111                32.0                 11.0           12 January 1988
                    YEAR V                  31.0                   4.0         16 December 1989
                    YEAR VIII               20.0                 16.0          17 December 1992
                    YEAR IX                 25.0                 15.0          14 December 1993






















                                                           164






















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               SrIE NUMBER - 55                              DESIGNATOR - M13DI

               SITE - DOG ISIAND, MATAGORDA BAY, TX

               NOMINAL SITE CENTER - 28038.28'N              95000.15'W

               L40CATED ON NOS CELART # - 11319

               SITE ACCESS - The launch point is at the public boat ramp on the Colorado River.
               Proceed northwest to the ICWW, then southwest along the ICWW to Culver Cut,
               go south to the first fork and then go east. Proceed to the second PVC pipe and
               then go south to the first iron structure which is 200 m from shore.

               SITE DESCRIPTION - This site was first sampled in Year III and was located in
               east Matagorda Bay near Dog Island Reef

               OYSTER COLLECTIONS

               Year III - The site was first established in Year III. Station I was an oyster shell
                     reef located approximately 500 m north of Dog Island Reef. Station 2 was
                     directly south of Station 1 and was located on the southeast end of Dog
                     Island Reef. There was a well developed reef with numerous oysters in the
                     intertidal zone. Station 3 was located 100 m to the southeast on the next
                     exposed reef. Oysters were also abundant at this site.

               Years IV, V, VI, VII, VIII, & IX - The site was not sampled during these years.

               SEDIMENT COLLECTIONS

               Year III - Sediments were collocated with the oysters at each station. Station 1
                     was 20 m north of the oyster reef. Sediment for Station 2 was collected 50 m
                     from the reef in -1.0 m of water. The sediment collection site at Station 3
                     was located 90 m to the north.

               Years X, V, VI, VII, VIII, & IX - No sediments were sampled during these
                     years.

               SAMPIJNG MEvMODS

                     Oysters - hand
                     Sediment - box core

               WATER DEPTH - intertidal, 0 to 0.3 m

               POSSEBLE CONTAM[INANTS - No apparent point sources of contamination were
               obvious. However, this site was located near Culver Cut which received drainage
               from agricultural activities, primarily farming of rice and grain sorghum. The
               Colorado River channel is scheduled to be diverted and will empty into this bay
               northeast of Dog Island Reef.



                                                       169




                                                                                                   I
             ENVIRONMENTAL DATA                                                                    I

                                    Salinity       Temperature      Dates Sampled
                                     (10/00)            (0c)                                       I
                 YEAR 111             28.0              6.0         11 January 1988                1
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              SrIE NUMBER - 72                               DESIGNATOR - BRCL

              SITE - CEDAR LAIKE, BRAZOS RIVER, TX

              NONONAL SITE CENTER - 28051.50'N               95027.83'W

              IA)CATED ON NOS CELART # - 11322

              SITE ACCESS - This intertidal site was first sampled in Year IV. A boat launch
              is located at the end of the road on FM 2918. From the boat ramp proceed
              southeast across the ICWW to the entrance at Cedar Lakes. Then proceed west-
              southwest down the channel for approximately 0.75 miles to where two land
              masses converge, one from the north and one from the south.

              SITE DESCREPTION - This site was located on the east side of the San Bernard
              National Wildlife Refuge near Rivers End, approximately 2 miles west of the San
              Bernard River. The site was south of the ICWW on the east side of Cedar Lakes.
              An old silted-in channel ran southwest off of the ICWW into Cedar Lakes. This
              area was predominated by very shallow bays with numerous oyster reefs
              surrounded by Spartina alterniflora marshland.

              OYSTERCOLLECTIONS

              Year rV - Station 1 was located 50 m southwest of the metal casing on the west
                     side of the small channel which turned to the south. Oysters were collected
                     by hand from an exposed reef on the west side of the channel. Station 2 was
                     located approximately 150 m northeast of the metal casing and 200 m from
                     Station 1. It was on the north side of the entrance channel. Oysters were
                     collected by hand from both sides of the land mass which protruded to the
                     south. Station 3 was located on the exposed reef which ran east-west and
                     paralleled the entrance channel. The actual collection site was 350 m east
                     of the metal casing and 200 m east-southeast of Station 2. Oysters were very
                     abundant at this location but the sediment was soft which made access to
                     the oysters difficult.

              Year V - Due to siltation of the channel leading to Station 1 it was moved 100 m to
                     the east. The station was located on the east side of the marsh that was
                     directly south of Station 2. Oyster collections were made on the small bay to
                     the south. Station 2 remained at the originally established sites. Station 3
                     was moved 100 m to the east for better access and better quality oysters.
                     This station was located on the west bank of the small channel which went
                     to the south and was approximately 300 ni east of Station 1.

              Year  VI - Oysters at Station 1 and 2 were collected in the same locations as in
                     Year IV. Station 3 was collected at the same location as last year, along the
                     small channel on the south side of the channel approximately 300 m east of
                     Station 1 and 2. Oysters were located on the west side of this channel.




                                                      175









               Year VII - Oysters were collected in the same area as last year but two stations
                      were moved. Station 1 was moved approximately 50 m to the east toward
                      Station 2. Station 2 was collected at the same location described for Year
                      IV. Station 3 was moved across the channel approximately 50 m to the
                      north of last years collection site.

               Years VIII, & LK - Oysters were collected at the same locations as in Year VII.

               ' 0MENT COLLECTIONS

               Year IV - Sediments were collocated with the oysters and were collected by hand
                      from within the oyster bed. The sediment was red-brown soft silty mud.

               Year V - Sediments were again collocated with each of the oyster sites and were
                      collected by hand. Sediment character was unchanged from last year.

               Years VI, VII, VIII, & IX - No sediments were sampled during these years.

               SAMPLING BEETHODS

                      Oysters - hand
                      Sediment - hand

               WATER DEPTII - intertidal, 0 to 0.3 m

               POSSIBLE CONTAMINANTS - No obvious contaminants were observed, a
               potential source is marine fuel and oil from the ICWW.

               ENVIERONM]KNTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (To)                 (0c)

                    YEAR IV                33.3                 16.0          29 November 1988
                    YEAR V                 27.0                 16.0          4 December 1989
                    YEAR VI                30.0                 12.7          5 December 1990
                    YEAR VII               19.0                 13.7          3 December 1991
                    YEAR VIII              24.0                 13.0          8 December 1992
                    YEAR EK                22.0                 18.0          7 December 1993















                                                           176
















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                                                               180
                                                                                            150               SAN       6ERNARD                                                                                              40
                                                                                              NATIONAL WILDLIFE REFUGE                                                                                                            A&
                                                                                                               (protected area)

                                                                                                                                                                                                                                                                               Marsh
                                                                                                                                                                                    u faced Romp

                                                                                                                                                                                                                        29
                                                                                                                                                     Snag,
                                                                                                                             Windmill
                                                                                                                                                                                                                                                                                    ee "Oles)
                                                                                                                                                                                                                                                                              CaSW.
                                                                                                                                                                                                                                                 27

                                                                                                                                                                                                                      P.
                                                                                                                                          pile.
                                                                                   Spo I Ar0a                                  or                                                                                                                  of
                                                                                                                                                                                                                        Pil
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                                                                                                                BRCL                                                                                                   KOF        FMARCATION J1Nr
                                                                                                                                                                                                                                           A)
                                                             Piles PA                                         Site #72
                                                                                                                                                                                                                                                                  -wl.

                                                                                                                                                                                                     . ...............                                                      .....
                                                                                                       go               OL -                                                    ........................                        3
                                                                                                                                                                                                            2          6
                                                                                                                                                             ................. *-
                                                  Q                                                                                                 ......                              4
                                                                                 ;                                                         ........                                                                               9
                                                                                                                    ............. ...........
                                                                                                          ...........                      2                                                                   9
                                                                          ........................                                                            8                           9                              12-                     PA                                          14
                                                                                                      Site #72 (BRCL), Brazos River Cedar Lakes (from chart 11322).,










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              SHE NUMBER - 57                               DESIGNATOR - BRFS

              SITE - FREEPORT SURFS11DE, BRAZOS RIVER, TX

              NOXINAL SITE CENTER - 28055.25'N              95020.33"W


              LOCATED ON NOS CELART # - 11322

              SITE ACCESS - This intertidal site was established in Year III and is accessed
              from the shore. From the intersection of FM 288 and 1495 drive south on FM 1495
              to the ICWW, cross the draw bridge and the site is located along the ICWW and in
              the housing development with silted-in boat basins east of FM 1495.

              SITE DESCRIPTION - Oysters could be collected easily by hand at low tide,
              otherwise it was necessary to tong for bottom concrete rubble on which the oysters
              were attached. A local resident indicated that oysters were less abundant in Year
              III, but a moderate population existed at the three stations.

              OYSTERCOLLECTIONS

              Year III - Year III stations were established as follows: Station 1 was directly
                     east of the highway along the concrete rubble on the south shoreline of the
                     ICWW and continued inside the first boat basin along the west side of the
                     bulkhead. Oysters were collected from concrete rubble and wood pilings on
                     the mud bottom. Station 2 was located across the boat basin along the east
                     side of the bulkhead. The oysters were attached to the concrete bulkhead
                     and from concrete rubble on the mud bottom. Station 3 was located in the
                     next boat basin to the east. Oysters were collected on the east side of the
                     channel along the bulkhead and from concrete rubble on the mud bottom.

              Years rV, V, VI, VII, & VIII - Oyster stations were collected at the locations
                     described for Year III.


              Year IX - The site was not scheduled to be visited in 1993.


              SEDIMENTCOLLECTIONS

              Year III - Sediments were collocated with the oyster stations. The bottom was
                     soft red silty clay over black to gray mud.

              Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                     years.

              SAWLINGMEMODS

                     Oysters - hand and tongs
                     Sediment - hand scoop and box corer




                                                      181









               WATER DEPTH - Water depth at all three stations was less than 1 m at high tide
                      with a range of 0 to 1 m, intertidal.

               POSSEBLE CONTAMINANTS - Contamination was possible from boat traffic in
               the ICWW and nearby oil and chemical refineries. Effluent from an adjacent
               dredge disposal site was returned to the ICWW through the second boat channel
               where Station 3 was located.


               ENVIRONMENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (9010)               (OC)

                    YEAR 111                32.0                 14.0          16 December 1987
                    YEAR IV                 30.7                 18.0          29 November 1988
                    YEAR V                  25.3                 12.0          4 December 1989
                    YEAR VI                 27.0                 13.8          5 December 1990
                    YEAR VII                15.0                 15.0          3 December 1991
                    YEAR VIII                -                     -















                                                                                                                                                                                ReEPOR
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                                                      451




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                                                                                                                                                                                  66









                                                                                                                                          INSET


                                                                               Site #57 (BRFS), Brazos River Freeport Surrside.







                                                                                                                                   FREEP               RT                                           to a dc0l' Of 9 leel lot 0 width of 50 feet.
                                                          OV"D ME 9 TEL CM
                                                          AUM C, 4,



                                                                   1                                                                          Do IDGE
                                                              F                                               Surface                         0 Ct". 11
                                                               /                                                      Ramp
                                                    FIXED BRIDGE                                               OV"D PWI CAI
                                                    HOR CL   100 F'                                            ALIT Ct So FT
                                                    VERT CL 50  F1
                                   Marsh                                                                                                                              Fr*opod Harbor
                                                                                                                                SW$MNO RRIDOE                 3      (Old Brazos lltioor)
                                                                                                                                "ON Ct 101 FT                           OV"D TEL CABS
                                                  pipefin                                                                       VERT Ct 11 "                            AUTH CIL 107
                                                    Area                                                                                 TANK                                  FIXED
                                                                                                                                                                         k     Hot C   too
                                                                                                                                                                          11   VERT C                                                 OVER"I
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                                                                                                                                                  MOR CIL 75 FT
                                                                                                                                                  VERT CL 60 FT
                                                                                                                                                                      Into r-Y EAI    0--
                                                                                                                                                                      UTH CIL 63 FT


                                                                                                                               N
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                                       AUTH rL SY                                                                                                                                               it       PA              ch ical I'larit
                                                                                                                                                                                                0041,                        1                   0 As
                                                                                       BRAZOS RIVER
                                                                        A depth of 8 feet at ordinary river stage                                          I                            /1U       19                        A   K,
                                                                     is  available upriver from the Intracoastal                                                                                                                          4K
                                                                     Woferwoy.                                                                                                                DOI 5                      T-iv 1I.I.,
                                                                                                           Rep. Aug. 1982                                                                                                                F it


                                                                                                                                                                                                       "22"
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                                                                                                                              Hot 0 130 FT V,
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                                                                                                                              AUTH Ct 100 FT                                                                                           . ..... ...........
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                                         Cur ent Indicator                                -40P                                                                                                                          111141          0
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                                                                                  Site #57 (BRFS), Brazos River Freeport Surfside (from chart 11322).










































































                    .Al









              SrIE NUMBER - 15                                DESIGNATOR - GBYC

              SITE - YACHT CLUB, GALVESTON BAY, TX

              NOM[INAL SITE CENTER - 29037.30'N               94059.50'W

              LOCATED ON NOS CHART # - 11326,11322

              SITE ACCESS - The collection site during Years I and II was a walk-up site just
              north of the Yacht Club. The site was moved in Year III to near the Judges Stand
              outside of the Houston Yacht Club. The site is accessed by boat launched at the
              Houston Yacht Club, Eagle Point Marina or Galley Restaurant Marina.

              SITE DESCRIPTION - The site was in the middle portion of Galveston Bay near
              Shoreacres just north of Red Bluff. In the first two years the site was along shore
              in front of the third house north of the yacht club, in from the retaining wall (Lat.
              29'37.20'N, Long. 95'00.10'W).       Oysters were intertidal and sediments were
              subtidal. Sediments were collected east of the Yacht Club property. Oysters were
              few in number and attached to rocks. In Year III, the site was moved. The new
              location at the Judges Stand was subtidal and oysters were more abundant.

              OYSTERCOLLECTIONS

              Years I & II - Years I & II shoreline stations were as follows: Station 1 was 50 m
                     north of the Yacht Club property. Station 2 was 70 m north of Station 1.
                     Station 3 was 80 m north of Station 2.

              Year III - There were no oysters along the shoreline in Year III; therefore all
                     three stations were moved to deeper water outside the entrance to the yacht
                     basin. The reef was 2 to 3 m deep and runs south from the judges stand
                     curving back to the shore. Station 1 was at the Judges Stand (a 4 piling
                     platform 300 m outside the yacht basin entrance). Station 2 was 100 m to the
                     south and Station 3 was another 100 m to the south.

              Year   IV - In Year IV all three oysters stations were collected by tong at the
                     Judges Stand (Station 1) due to rough weather conditions and siltation of
                     the lower end (Station 3) of the reef.

              Year V - Oysters were collected from the reef around the Judges Stand.

              Year W - Oysters were again collected by tong from around the Judges Stand and
                     were abundant despite the high volume and long duration of freshwater
                     experienced in Galveston Bay.

              Year VII - Oysters were again collected by tong from around the Judges Stand.

              Year VIII - Oysters were collected by dredge from around the Judges Stand.

              Year IX - The site was not visited this year.



                                                       187










                   )IMENTCOLLECTIONS

               Years I & II - Sediments were collected just offshore (100 m) from the oysters
                      stations.

               Year III & IV - No sediments were collected these years.

               Year V - Sediments was also collected around the Judges Stand, with Station 1
                      about 50 m to the north, Station 2 was 25 m south of the stand. Station 3 was
                      75 m southwest of station 2.

               Years VT VII, VIII, & IX - No sediments were sampled during these years.

               SAMPLING AMEYMODS

                      Oysters - hand, tong, or dredge
                      Sediment - box core

               WATER DEPTH - intertidal, 0.5 m (Years I & II); subtidal, 1.5 - 2.0 m.

               POSSIBLE CONTAMINANTS - Contamination factors included ship traffic,
               residential pesticides, heavy industry and city sewage.

               ENVIRONAERWAL DATA

                                          Salinity         Temperature         Dates Sampled
                                            (9010)               (OC)

                    YEAR 1                  15.0                 12.0          12 January 1986
                    YEAR 11                 10.0                 13.0          16 December 1986
                    YEAR 111                24.0                 17.0          11 December 1987
                    YEAR IV                 22.0                 16.0          30 November 1988
                    YEAR V                  20.0                 13.0          5 December 1989
                    YEAR VI                 24.0                 14.0          6 December 1990
                    YEAR VII                12.0                 15.0          4 December 1991
                    YEAR VIII               17.3                 13.0          9 December 1992


















                                                           188















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                                                      Site #15 (GBYC), Galveston Bay Yacht Club.


      C)SheareT Publishing Company. Reprinted by permission.















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                                y platforms, signs,        pipes, piles, and                                                                                                                   Fl G Asac l7ft '85'                       4"C 404 '86;
                                s, some submerged,         may exist along thf                                                               I .                              - P0.            W Or                                      - fka
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                                porcry changes or defects in aids to                                                                                                          Ab @a ed Slu                                               Wo, Priv maimed
                                                                                                                                       30 ft                                                   pd@ .001d                                 A%7
                                tion are not indicated on this chart.                                                                  MP 1982       C"2                                       a                                         0
                                tice to Mariners.                                                                                                                                                                                        7Ok FI '75'
                                                                                                                                                     GBYC                                      R                                         riling.
                                roved channels shown by broken lines                                                 CAUTION                                                                   8                                         3@ WO's
                                bject to shoaling, particularly at the                               Submerged Pipeline a              md Ccibles    Site #1                                                                             Priv mointol
                                                                                               Uncharted submerged pipelines and cables                                                        .Pile                                     C
                                                                                            may exist in the vicinity of gas and oil -ell                                                                                                3aOk lel C
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                                                    S -1 . -                               I      .,
                                                                                                                                                                                                                                         7

                                                    Site #15 (GBYC), Galveston Bay Yacht Club (from chart 11326).












              SITE NUMBER 16                                DESIGNATOR - GBTD

              SITE - TODD'S DUMP, GALVESTON BAY, TX

              NONEINAL SITE CENTER - 29030.06'N             94053.82W

              LOCATED ON NOS CHART # - 11326,11322

              SITE ACCESS - The boat was launched at Pelican Island (TAMU boat basin) in
              Years I and II and the run up the Houston ship channel to San Leon takes about 1
              hour. In Year III, the boat was launched at San Leon Marine near Eagle Point
              where a lift was available. In subsequent years the boat was launched at the
              Eagle Point Marina.

              SITE DESCRIPTION - The collection site was near an exposed reef midway
              between southeast Eagle Point and Red Fish Island at Houston Ship Channel.
              The reef ran from Eagle Point to Red Fish Island. This was an extensive reef with
              several shipwrecks. The top of the reef was exposed at low tide.

              OYSTER COLLECTIONS

              Years I & Il - Station 1 was west-southwest of the reef between the reef and Eagle
                    Point in 1.5 m of water. Bearing to landmarks are 850 to middle exposed
                    reef-, 180' to Railroad draw bridge. Station 2 was dredged near the exposed
                    portion of the reef (about halfway between the marina and the ship channel
                    dredge material islands). Station 2 landmarks were 140' to the middle of
                    the exposed reef. Station 3 was dredged northeast of the reef at about half
                    the distance to the island. Station 3 landmarks are 330' to the middle of
                    exposed reef-, 210' to railroad draw bridge.

              Year III - Year III stations were close to those previously used. Station 1 was
                    moved approximately 0.5 miles to the north -50 m offshore of the exposed
                    reef which borders the ship channel and east of the south end of the reef.
                    Stations 2 and 3 were the same as those previously sampled.

              Year  IV - In Year IV the oysters were obtained from the north side of the
                    east/west reef extending from Eagle Point marina to the exposed reef
                    bordering the ship channel. The location corresponds to Station 2 in
                    previous years. At the time of sampling all commercial oystering was
                    confined to this area and the oysters were purchased.

              Year V - Station 1 oysters were purchased from a lugger working on the east end
                    of the reef. Station 3 oysters were obtained from another lugger working on
                    the west end of the reef, approximately 200 m from the first pole at the
                    entrance to the Eagle Point Marina. Station 2 was a combination of oysters
                    from Stations 1 and 3.

              Year VI - Station 1 oysters were collected at the high point of the reef toward the
                    west end. Station 2 was collected at the middle of the reef near the ship









                     wrecked boat (29'29.70'N, 94*53.82'W). Station 3 was dredged by a
                     commercial lugger operating slightly north of the east end of the reef in the
                     bend of Red Fish Island.

              Year   VII - Station 1 oysters were collected on the northwest end of the reef
                     approximately 200-300 m from the exposed portion of the reef, Station 2 was
                     near the middle of the reef approximately 300 m north of the wrecked
                     shrimp boat. Station 3 was on the east end of the reef approximately 500 m
                     northeast of the shrimpboat. Oysters were numerous at the first two
                     stations.

              Year VIII - Oysters were collected at the same reef segments as last year.

              Year IX - Large oysters were abundant all the way along the reef. Station 1
                     oysters were collected on the south side of the reef, next to the sunken
                     shrimp boat. Station 2 oysters were collected 300 m west of the wreck, also
                     on the south side of the reef. Station 3 oysters were collected 300 m east of
                     the wreck on the south side of the reef.

                  )DIENTCOLLECTIONS

              Years I & II - Sediments in Years I and II were collected north and northwest of
                     the reef. Mud bottoms were patchy in distribution.

              Year III - Sediments were collected within 100 m of each of the oyster sites.

              Year IV - Sediments for Year IV were collected with a box corer within 100 in of
                     each of the oyster collection sites described for Year III.

              Year V - Sediments were collected adjacent to each station. Station 1 was 100 m
                     north of the east end of the exposed reef. Station 2 was 100 m north of the
                     center of the reef. Station 3 was 300 m north of the oyster collection site. All
                     the sediments were sandy-silt over black clay.

              Year VI - Sediments (NIST) were collected 100 m north of each oyster station.

              Years VII, VIII, & IX - No sediments were sampled during these years.

              SAMPLING METHODS

                     Oysters - Dredging, tonging, or by hand
                     Sediment - box core

              WATER DEPTH - intertidal to subtidal, 0.1 to 3 m

              POSSIBLE CONTAMINANTS - Sources of contamination included the dredging
              activities on the Houston ship channel, the Houston ship channel-Galveston Bay
              complex, and the petrochemical industry at Texas City.



                                                        194











                 ENVIRONMENTAL DATA

                                              Salinity            Temperature           Dates Sampled
                                                0700)                   (OC)

                       YEAR 1                   12.0                      9.0           12 January 1986
                       YEAR 11                    8.2                   11.0            19 December 1986
                       YEAR 111                 30.0                    18.0            9 December 1987
                       YEAR IV                  33.0                    16.0            29 November 1988
                       YEAR V                   19.0                    14.0            6 December 1989
                       YEAR VI                  22.0                    13.0            7 December 1990
                       YEAR VII                 10.0                    13.2            5 December 1991
                       YEAR VIII                13.3                    18.0            10 December 1992
                       YEAR IEK                 13.0                    16.0            8 December 1993









































                                                                 195





























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                                                  Site #16 (GBTD), Galveston Bay Todd's Dump.












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                                                     Site #16 (GBTD), Galveston Bay Todd's Dump (from chart 11326).









                                                                                     6r,









               SrIE NUM]BER - 17                             DESIGNATOR - GBHR

               SITE - HANNA REEF, GALVESTON BAY, TX

               NONIINAL SITE CENTER - 29028.85'N             94044.00W

               U)CATED ON NOS CHART # - 11326,11322

               SITE ACCESS - The boat was launched at TAMU boat basin on Pelican Island
               during the first two years of the study and the ICWW is traveled along Bolivar
               Peninsula to the first cut (Sievers Cove) through to East Bay. The reef is to the
               northwest. In Years III & IV, the boat was launched at the Eagle Point Marina.

               SITE DESCRIPTIONS - Hanna Reef is a large subtidal reef that runs northwest to
               southeast and separates East Bay from Galveston Bay. The large exposed (only at
               low tide) portion in the middle served as the point of reference for site
               identification. The oyster site was the submerged reef northeast of the large
               exposed part (bearing 055' from) and marked by two telephone pole sized pilings.
               Also at mid-bay there was a solitary concrete piling and a six leg wooden
               platform. The area was fished commercially and marked by many temporary
               stakes.


               OYS= COLLECTIONS

               Year I - In Year I the oysters were obtained on site from a commercial dredger
                      and thus no station distinction was possible.

               Year II - In Year 11 the stations were dredged from the submerged reef to the
                      northeast of the exposed part of Hanna Reef. Station I was on the west side
                      of the submerged reef, between two telephone poles. Station 2 was north of
                      the reef. Station 3 was east of the reef.

               Year   III - In Year III, oysters were purchased from a commercial fisherman
                      operating at Stations 1 and 2 and oysters were dredged from Station 3 at
                      approximately the same location described above.

               Year   IV - Oysters were purchased from commercial fishermen dredging on
                      Hanna Reef.

               Year V - Oysters were tonged to the north of the exposed part of Hanna Reef on a
                      line between the white oil platform and the six legged wood platform.
                      Station 1 was on the west side of the reef approximately 1000 m east of the
                      white platform. Station 2 was 150 east of Station 2 near the top of a
                      submerged reef. Station 3 was another 200 m east toward the six legged
                      platform. The bottom was shell and shell hash in dark brown mud.

               Year   VI - Oysters were purchased from a commercial fisherman dredging on
                      Hanna Reef.





                                                       201









               Year VII - All three stations were dredged off of the east end of the exposed
                      portion of Hanna reef. The first station was approximately 300 m east of the
                      reef with Stations 2 and 3 further to the east separated by approximately
                      50-m.

               Year VIII - Oysters were dredged in the same location as Year VII.

               Year IX - This site was not scheduled for collection this year.

                   )DONTCOLLEMONS

               Years I, II, & III - Sediments were obtained by boxcore adjacent to the oyster
                      stations.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.

               SAMPLING ME"MODS

                      Oysters - dredge, tong, or purchase
                      Sediment - box core

               WATER DEPTH - subtidal, 1 to 3 m

               POSS11BLE CONTAMINANTS - Possible contaminant sources included oil and
               gas development in Galveston and East Bays and the petrochemical complex at
               Texas City.

               ENVIRONAIENTAL DATA

                                         Salinity           Temperature         Dates Sampled
                                            (9100)               (OC)

                    YEAR 1                  10.5                   7.0         11 January 1986
                    YEARII                  10.0                 12.0          16 December 1986
                    YEAR 111                30.0                 18.0          9 December 1987
                    YEAR IV                 34.0                 16.0         29 November 1988
                    YEAR V                  16.3                 14.0          6 December 1989
                    YEAR VI                 23.0                 12.0          6 December 1990
                    YEAR VII                10.0                 13.2          4 December 1991
                    YEAR VIII               18.0                 13.0          10 December 1992












                                                           202














                                                                                                                         77





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                                                               Site #17 (GBHR), Galveston Bay Hanna Reef.





       OShearer Publishing Company. Reprinted by permission.














                                                                            2
                                                            %Fingt-et.u.           1.                                                  ing the regulations may be obtained at the
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                                                                                                                                       Office of the District Eng;neer, Corps of
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                                                                                                                                                                                                        oined
                                                                                                                                         Anchorage regulations may be obi                                                                                              - --- ------
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                                                    2.%19                                                                              Guard District in Ne- Orleans, Lo.
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                                                     Site #17 (GBHR), Galveston Bay Hanna Reef (from chart 11326).











               SrIE NUMBER - 18                                DESIGNATOR - GBCR

               SITE - CONFEDERATE REEF, GALVESTON BAY, TX

               NONIINAL SITE CENTER - 29015.75'N               94054.88V

               LOCATED ON NOS CHART # - 11326,11322

               SITE ACCESS - The site is accessed from the boat launch at TAMU boat basin or
               more directly by driving to a public boat ramp for launch directly south of the site.
               Take Seawall Blvd. west to 8 Mile Road, which joins Anderson Ways Road at
               Stewart Street. Continue on Anderson to the north almost to West Bay and turn
               left at Sportsman Road, proceed for -1 mile. The boat ramp is on the right
               between two houses.

               SITE DESCRIPTION - The site was a reef between Deer Island and shore,
               approximately 0.5 mile from the boat ramp. This large reef was exposed at low
               tide and was utilized by commercial and private fisherman. This was a heavily
               utilized reef, as was evidenced by the large number of people collecting oysters
               during the third year's collections.

               OYSTER COLLECTIONS

               Years I, II, & III - Stations were established along the edge of the reef. Station 1
                      was located on the eastern tip of the reef. Station 2 was in the middle of the
                      reef and Station 3 was at the west end. Oysters were picked by hand from
                      0.5 m of water on both sides of the reef.

               Year   IV - In Year IV, the prevailing winds and tides had covered the reef
                      completely with waves of about two feet. Thus, sampling by hand on the
                      reef margins was not possible. Oysters were obtained from a commercial
                      dredger operating in the area of former Station 2, between Confederate Reef
                      and South Deer Island to the north. The site was 1.7 nautical miles to the
                      west from the green channel marker 9 at the entrance to Offatts Bayou.

               Years V, VI, VII, & VIII - Oysters were collected by hand at low tide from the
                      exposed reef. Station 1 was located on the eastern tip of the reef where 50 m
                      of reef was above water. Station 2 was in the middle of the reef to the west of
                      Station 1. Station 3 was on the west end of the reef approximately 300 m
                      west of Station 2. The entire reef was over hard bottom composed of shell
                      and shell hash.

               Year LX - The site was not scheduled for collection this year.

               SEDIM[ENT COLLEMONS

               Years I, II, & III - Sediments were patchy and were located approximately 1 mile
                      north of the oyster stations, between Deer Island and the reef, and




                                                        2T










                     northeast from the island at a distance of -50 m. Sediments were collected
                     at Lat. 29'16.10'N and Long. 94'54.60'W.

              Year V - Sediments were collected north of the oyster stations near Deer Island.
                     Station 1 was on the east side of the island 200 m from shore. Station 2 was
                     on the south shore of Deer Island near a cove approximately 60 m from
                     shore. Station 3 was on the west side of Deer Island 60 m from the shore.
                     Sediments were silty sand over hard sand.

              Years IV, VI, VII, VIII, & IX - No sediments were sampled during these years.

              SAMPLINGMETHODS

                     Oysters - hand
                     Sediment - box core

              WATER DEPTH - intertidal or subtidal, 0 to 0.5 m

              POSSIBLE CONTAMINANTS - There were no obvious sources of contamination
              in the vicinity of the reef.

              ENVIRONMIENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                 (0c)

                   YEAR 1                  20.0                   7.0          10 January 1986
                   YEAR 11                 17.0                 14.0          16 December 1986
                   YEAR 111                37.0                 18.0          10 December 1987
                   YEAR IV                 40.0                 12.0          30 November 1988
                   YEAR V                  21.5                 11.0           5 December 1989
                   YEAR VI                 26.0                 13.0           6 December 1990
                   YEAR VII                24.0                 12.0           4 December 1991
                   YEAR VIII               23.6                 13.0           9 December 1992










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                                                         Site #18 (GBCR), Galveston Bay Confederate Reef.





          eShearer Publishing Comvanv.             Reprinted by permission.













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                                                                         Site #18 (GBCR), Galveston Bay Confederate Reef (from chart 11322).









                                                                                    NA,









                SrIE NUMBER - 58                              DESIGNATOR - GBOB

                SITE - OFFATTS BAYOU, GALVESTON BAY, TX

                NONIINAL SITE CENTER - 29017.08'N             94050.15V

                LOCATED ON NOS CHART # - 11322

                SITE ACCESS - This collection site, established in Year III, is located in the
                middle of the south shore of Offatts Bayou. From Stewart Rd. Take 71st Street
                north to Heards Lane and then travel down Bay Drive through the apartment
                complex. Follow the dirt road around the turn for approximately 100 m and turn
                north onto private property to an open shed with a green roof The stations were
                located along the shoreline north of the shed at Lat. 29016.65'N and Long.
                94'50.87'W. In Year IV, the collection site was moved to the 61st bridge at the
                public boat ramp.

                SITE DESCRIPTION - This collection site was located in the middle of the south
                shore of Offatts Bayou. Oysters could be picked up along the shoreline but
                sediments had to be obtained in deeper water east of the oyster stations. Oysters
                were fairly common but not abundant and were attached to concrete and brick
                rubble or were loose on the bottom.


                OYSTERCOLLECTIONS


                Year III - Year III stations were located as follows: Station 1 was located at the
                      boat ramp and extended east to the first set of wooden pilings. Station 2 was
                      located between the pilings and wood pier. Station 3 was located to the east
                      of the wood fishing pier.

                Years X, V, & VI - In Year IV, the Offatts Bayou site was moved to the east end
                      of the bayou to correspond to the location from which sediments were
                      sampled in Year III. The new GBOB stations were located on the 61st
                      Street causeway at the bridge which crosses the bayou. Station 1 was on the
                      bay side of the bridge, extending from the bridge to the public launching
                      ramp. Station 2 began under the bridge (on the south side) and extended
                      into the enclosed basin part of the bayou. Station 3 was inside the small
                      basin, extending south from the bridge.

                Years VII, VIII, & IX - Oyster collection stations were located at the same sites
                      described above.


                     MENT COLLECTIONS

                Year III - Sediment was collected at the east end of Offatts Bayou near the
                      shoreline. This was the only place in the bayou where mud was found. All
                      other sites had sandy bottoms.




                                                        213









              Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                    years.

              SAMPLINGMETHODS

                    Oysters - hand
                    Sediment - box core

              WATER DEPM - 0 to 0.5 m.

              POSSIDBLE CONTAMINANTS - No obvious point sources of contamination were
              seen; however, this bay was surrounded by housing and residential development
              and had a long-term history of pollution.

              ENVIRONMENTAL DATA

                                        Salinity          Temperature         Dates Sampled
                                          (9010)               (0c)

                   YEAR 111               35.0                 18.0          8 December 1987
                   YEAR IV                38.6                 15.0         30 November 1988
                   YEAR V                 21.7                 13.0          5 December 1989
                   YEAR VI                27.3                 15.5          6 December 1990
                   YEAR VII               24.0                 14.5          4 December 1991
                   YEAR VIII              25.0                 15.0          9 December 1992
                   YEAR IEK               21.0                 17.0          8 December 1993































                                                         214











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                                                         Site #58 (GBOB), Galveston Bay Offatts, Bayou.


       10Shearer Publishing Company. Reprinted by permission.















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                                                                                                  Site #58 (GBOB), Galveston Bay Offatts Bayou (from chart 11322).



































































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                SITE NUMBER - 59                                DESIGNATOR - GBSC

                SITE - SHIP CHANNEL, GALVESTON BAY, TX

                NONEINAL SrIE CENTER - 29042.27'N               94059.58V

                LOCATED ON NOS CELART # - 11326

                SITE ACCESS - This collection site, which was established in Year III, is located
                at the mouth of Goose Creek in Tabbs Bay. Access to the site is by boat launched at
                The Galley Restaurant and Marina, south of U.S. Highway 146 on Black Duck
                Bay.

                SM DESCRIPTION - The three oyster stations were located approximately 150 m
                off of Tabbs Bay point in a south to north line on a submerged reef marked by
                concrete pilings. Oysters were collected with tongs in I to 1.5 m of water on the
                submerged reef Numerous dead oysters were present along the reef but
                abundant young oysters were present.

                OYSTER COLLECTIONS

                Years 111, X, VI, VI, WI, & Wrl - Oyster stations in all years were from south
                       to north along the reef Station 1 was near a group of concrete pilings.
                       Station 2 was 50 m north and Station 3 was 50 m farther north.

                Year IX - Very few live oysters were to be found in the area, as a layer of silt now
                       covers the dead reef. Only one small sample of oysters was collected from
                       the entire area that was originally established in Year III.                 The
                       surrounding area was searched and no live oysters were found, just
                       extensive collections of oyster shell.

                SEDIMENT COLLECTIONS

                Year III - Sediment stations were located east of the oyster stations. Station 1 was
                       collocated with the first oyster station. Station 2 was located 50 m to the east
                       and Station 3 was 50 m farther east.

                Year X - No sediments were collected this year.

                Year V - Sediment samples were taken in the same locations as described in Year


                Years V1, VII, VIII, & IX - No sediments were sampled during these years.

                SAWLWG M=ODS

                       Oysters - tongs and dredge
                       Sediment - box core





                                                          219









             WATER DEPTH - subtidal, 1 to 1.5 m

             POSSIEBLE CONTAMINANTS - Potential contamination was possible from the
             Houston Ship Channel to the west and from the Goose Creek watershed which
             drained a heavily industrialized area containing several petrochemical
             refineries.

             ENVIRON7vfENTAL DATA

                                        Salinity         Temperature         Dates Sampled
                                          (%0)                 (OC)

                  YEAR 111                22.0                 18.0         12 December 1987
                  YEAR IV                 21.0                 16.0         30 November 1988
                  YEAR V                  17.0                 13.0          5 December 1989
                  YEAR VI                 10.0                 16.0          6 February 1991
                  YEAR VII                12.0                 16.0          4 December 1991
                  YEAR VIII               15.0                 14.0          9 December 1992
                  YEAR IX                 12.0                 16.0          7 December 1993











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                                                               46


                                                                       Site #59 (GBSC), Galveston Bay Ship Charmel.



              a4-hpnrp@r Plubh-hinv Comloanv. Reprinted by permission.















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                                                 CAUTION                                                                                          I            mAR 978-FES 1979                                   @PLA-@,rlil I
                                                                                                                                      4                                       -%b" "ta ed Oil                     .-M              War
                                 porary changes or defects in a;ds to                                                                             30 fly                                                          P- :riamIdA
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                                                                                                                                                                                                                                                                 7Ok FI -75-
                                 tice to Mariners.                                                                                                                                                                R
                                 roved channels shown by broken lines                                                           CAUTION                                                                           81 ff                                          wof 8
                                                                                                                                                                                                                                                                 priv moined
                                 bject to shoaling, particularly at the                                       Submerged Pipeline and Cables
                                                                                                        Uncharted submerged pipelines and cables                                                                  1:7 file                                       C
                                                                                                     -may exist      in the vicinity of gas and oil well                                                                                                         8 10k 1`1 C
                                                                                                     structures, or bei@veen such s1ructu'es and the Surf Oo s                                                                                                   FI Aw
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                                                                                                     anch                                                                                                                                                        7S.
                                                                                                     $     >,ing                                                           / -        I.                                                                         .:                      I
                                                       Site #59 (GBSC), Galveston Bay Ship Channel (from chart 11326).











                                               LOUISIANA SrIES



               SHE NUMBER - 19                                 DESIGNATOR - SLBB

               SITE - BLUE BUCK POINT, SABINE LAKE, LA

               NOWNAL SITE CENTER - 29047.50'N                 93054.42W

               LOCATED ON NOS CHART # - 11342

               SITE ACCESS - The site is accessed by boat launched at the ramp on the east side
               of Sabine Lake at the base of the Highway 82 bridge at Mesquite Point. The ramp
               is on the right just after crossing into Louisiana. The site is north of the ramp at
               Blue Buck Point about a 10 minute run by boat. In some years, there were
               numerous gill nets in the area.

               SITE DESCRIPTION - The site was at or adjacent to Blue Buck Point in the lower
               part of Sabine Lake. Oysters have been collected by hand along the shoreline and
               by tonging in deeper water offshore from the point. Oysters occured in clumps on
               the shell and mud bottom and were not overly abundant.

               OYSTERCOLLECTIONS

               Year I - Oysters were collected by dredge and by hand the first year. Station 1 was
                      dredged 300 m. north of Blue Buck Point at a bearing of 3600 from the point,
                      1100 from the middle of the ICWW bridge, and 340' from the tall radio tower
                      to the south. Station 2 was collected by hand just offshore approximately
                      80-ni south of the point. Station 3 was 350 ni south of Station 2 about 10 ni
                      offshore.

               Year II - Collection sites were along the shoreline . Station 1 was collected along
                      the western margin of the lake at Blue Buck Point approximately 1 to 10 m.
                      offshore. Station 1 was a composite of Stations 2 and 3 since the collectors
                      were unable to find oysters at last years station. Station 2 was just south of
                      the point and Station 3 was approximately 200 m farther south.

               Year III - Oysters were tonged 200 m offshore with Station 1 off the western edge
                      of Blue Buck Point. Station 2 was south of the point and Station 3 was 200 ni
                      farther south. Oysters were scatter clumps in the sandy mud bottom.

               Year IV - Year IV Stations were located slightly farther offshore than previously
                      sampled. Station 1 was 250 m south of the duck blind at Blue Buck Point.
                      Station 2 was 400 ni southeast of the duck blind and 250 ni south of Station 1,
                      located at a pole and approximately due west of several oil tanks on the
                      shore. Station 3 was 250 ni south of Station 2. Numerous oysters were dead
                      or near death, the reef appeared to have had a partial die-off, and appeared
                      to be recovering.




                                                        225





                                                                                                                 I


               Years V & V1 - Oysters were located along a narrow reef with a few live oysters
                      attached to dead clumps. Station I was 300 m west of the point. Station 2
                      was 100 m south of the point. Station 3 was 150 m south of station 2.

               Year VII - Oysters were collections were at the same stations as the previous two
                      years. Oysters were very sparse with many recently dead.

               Year V111 & IX - Oysters were collected at the same stations as in the Years V
                      through VII

                    SEDIMENT COLLECTIONS

               Years I, II, & III - Each year the sediments were collected offshore in subtidal
                      waters adjacent to the respective oyster sample stations.

               Years IV & V - No sediments were collected during these years.

               Year VI - Sediment sites for NIST sediments were collocated with each oyster
                      station.

               Years VII, VIII, IX - No sediments were sampled during these years.


               SAMPLING METHODS

                                                                                                                  
                      Oysters - hand, tong and dredge
                      Sediment - box core


               WATER DEPTH - 0.0 - 1.5 meter

               POSSIBLE CONTAMINANTS - Contamination factors included ship traffic,
               urban sewage, and heavy industrialization nearby.

              ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%)               (c)

                    YEAR I                 17.5                  7.0         26 January 1986
                    YEAR II                19.0                  3.0         10 December 1986
                    YEAR III               15.0                 16.0         28 December 1987
                    YEAR IV                16.0                 27.0          6 December 1988
                    YEAR V                 20.0                 14.0          6 December 1989
                    YEAR VI                15.0                 13.0          8 December 1990
                    YEAR VII               10.0                 14.0          5 December 1991
                    YEAR VIII              16.0                 12.0         10 December 1992
                    YEAR IX                11.0                 16.0          9 December 1993






                                                          226

















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                                             Site #19 (SLBB), Sabine Lake Blue Buck Point.




       CShearer Publishing Company. Reprinted by permission.

















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                                        Site 019 (SLBB), Sabine lake Blue Buch Point (frem chait 12342).
































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               SrIE NUMBER - 20                               DESIGNATOR - CLSJ

               SITE - ST. JOHNS ISLAND, CALCASIEU LAKE, LA

               NONGNAL SrIE CENTER - 29049.83N                93023.00V

               LOCATED ON NOS CHART            11347

               SITE ACCESS - The boat is launched at the ramp in Calcasieu Channel at
               Cameron. The ramp is located on the west bank of the channel at the ferry. Run
               time to the site is 0.25 hours by water. Travel to the site by going north to West
               Pass and then west along West Pass until its entrance into West Cove.

               SITE DESCRIPTION - The site was in West Cove near the mouth of West Pass,
               between the shoreline and Rabbit Island.

               OYSTER COLLECTIONS

               Year I - Year I oyster Station 1 was 150 m northwest of St. John's Island on the
                      submerged reef. Bearings to Station 1 was 60' to tall channel marker; 295'
                      to near point mainland west. Station 2 was located 55' to tall channel
                      marker; 2951 to near point of mainland to the west. Station 2 was 100 m
                      northeast of Station 1 and 200 m from St. John's Island. Station 3 was
                      taken 150 in west of Station 2 and 250 m east of St. John's Island. Oysters
                      were abundant at this site.

               Years  II, III, & IV - Oyster stations were at the same general locations as
                      previously sampled. In Year IV, oysters were fairly abundant at the first
                      two stations and near the shore, but were silted over away from the shore.

               Years V, V7, VII, & VIII - Oysters stations were in the same general locations all
                      four years. Station 1 was at the north end of St. Johns Island. Station 2 was
                      on the shoreline 250 m from station 1. Station 3 was 150 m northwest of
                      Station 2.

               Year IX - This site was not scheduled for sampling this year.

               SEDIN[ENT COLLECTIONS

               Years I, II, & III - Sediment at Station 1 was 50 m from the first oyster station on
                      the reef. Station 2 sediments were adjacent to the reef to the northeast.
                      Station 3 sediments were located 40 m west of the oysters.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.








                                                        231










                      Oysters - tongs and dredge
                      Sediments - box corer

               WATER DE= - I to 2.5 m

               POSSIBLE C(DHTAbMMNTS - No contamination sources were observed.


               H9qVMC)NhEEHTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (0/00)               (OC)

                    YEAR 1                 13.3                 15.0           18 January 2986
                    YEAR 11                  9.3                12.0           9 February 1987
                    YEAR 111               23.0                 16.0          15 December 2987
                    YEAR IV                35.0                 15.0           7 December 1988
                    YEAR V                 26.7                 16.0           7 December 1989
                    YEAR VI                27.3                 13.0           7 December 1990
                    YEAR VII               17.0                 14.0           6 December 2991
                    YEAR VIN               17.0                 17.0          11 December 1992






















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                                                           232

















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                SITE NUMBER - 60                              DESIGNATOR - CLLC

                SITE - LAKE CHARLES, CALCASIEU LAKE, LA

                NOMINAL SITE CENTER - 30003.42'N              93018.42'W


                LOCATED ON NOS CHART            11347

                SITE ACCESS - Access to the site is via LA Highway 384 to Deatonville and a boat
                launch at the Cajun Cove Marina. The channel going out from the launch is very
                shallow at low tide. From the launch proceed northwest along the shoreline for
                 700 m to a long pier at the Reef Restaurant. In Year V the site was moved, so
                -after launching proceed on a bearing of 80' from the ramp to the shell island. The
                GIWW bridge is due west of the site. Since the site was moved it is much easier to
                launch the boat at the Ellender Bridge and go up the GIWW to the north end of
                Calcasieu Lake.

                SITE DESCRIPTION - This collection site, established in Year III, was located in
                the north end of Calcasieu Lake at East Pass (30'03.50'N and 93'17.50W). Oysters
                were scattered on a submerged (0.75 to 1 m deep) muddy reef, with individual
                clumps of 3-10 oysters. Oysters were thin-shelled and broke easily when
                separated. In Year V the site was moved 1 mile to the west to an oyster shell reef
                and nearby Spartina alterniflora marshes.

                OYSTER COLLECTIONS

                Years III & X - Year III oyster Station 1 was located 30 m south of the end of the
                      Reef Restaurant Pier. Landmarks to locate this site were 2700 to the twin
                      towers of the Ellender Lift Bridge and 1340 to the Cajun Cove Marina ramp.
                      Station 2 was 75 m southeast of Station 1 approximately 30 m off of the
                      private piers. Station 3 was another 75 m southeast, 30 m off of the last
                      private pier.

                Year V - The collection site was relocated about a mile west due to the lack of live
                      oysters at last year's site. The new site is a oyster shell island with marsh
                      grass nearby. Oysters can be collected by hand during low tide. Station 1
                      was located on the south end of the shell reef just to the north of the
                      Spartina island. Station 2 was 75 m to the north and Station 3 was 100 m
                      west of Station 2.

                Years VI & VII - Oysters were collected at the same stations described in Year V.
                      There is a new orange metal post marker on the north end of the reef. An
                      oyster die off has occurred since last year, only small oysters were present.

                Year  VIII - Oysters were collected at the same stations described in Year V.
                      Oysters were more abundant and larger than the previous two years.




                                                        237








             Year IX - Large oysters were abundant across the reef The same stations were
                    used as those described in Year V.

             SEDIMENT COLLECTIONS

             Year III - Sediments were a light gray-brown, fine-grained clayey layer over a
                    dark brown clay. Collection sites were collocated with each oyster site.

             Years IV & V - No sediments were sampled during these years.

             Years VI - NIST sediments were collected 50 m from the oyster stations in 1.5 m
                    of water.

             Years VII, VIII, & IX - No sediments were sampled during these years.

             SAMPLINGMETHODS

                    Oysters - tongs and hand
                    Sediment - box core

             WATER DEPTH - intertidal, 0.2 to 0.5 m

             POSSIBLE CONTAMINANTS - Potential local contamination was from private
             piers, septic tanks, oil production, and boat traffic in the ICWW. However, since
             the site was located at the mouth of the Calcasieu River, there was a much
             greater potential for contamination from upstream discharges particularly from
             the Lake Charles metropolitan area.

             ENVIERONMENTAL DATA

                                        Salinity           Temperature         Dates Sampled
                                           (9010)               (OC)

                  YEAR 111                 15.0                 11.0          16 December 1987
                  YEAR IV                  28.0                 16.0          7 December 1988
                  YEAR V                   22.0                 15.0          7 December 1989
                  YEAR VI                  23.0                 15.0          8 December 1990
                  YEAR VII                 12.0                 16.0          6 December 1991
                  YEAR VIII                14.0                 13.0          10 December 1992
                  YEARIX                   12.0                 18.0          9 December 1993














                                                          238


















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                        Site #60 (CLLC), Calcasieu Lake Lake-Charles (from chart 11347).












                                                                   ell.                                                                                                L .41









              SITE NUMBER - 21                              DESIGNATOR - JHJH

              SITE - JOSEPH HARBOR BAYOU, J. HARBOR BAYOU, LA

              NOAENAL SITE CENTER - 29037.75'N              92045.75W

              LOCATED ON NOS CHART            11345,11344

              SITE ACCESS - Access to the site is by boat launched at the Gran Chenier Boat
              Ramp on Highway 82, west of North Island canal. Proceed by boat south along a
              man-made channel to the mouth of Joseph Harbor Bayou. Run time to the site is
              approximately 0.5 hrs.

              SITE DESCRIPTION - The collection sites were located on both sides of the
              channel; 300' from the Gulf along the grassy margins of the bayou east of Miller
              Lake. Oysters were taken from intertidal waters from a mud and shell bottom.
              Low tide was the optimum collection time.

              0YSTER COLLECTIONS

              Year  I - Year I stations were established as follows: Station 1 oysters and
                    sediments were collected from the east bank of the bayou 200 m north of the
                    entrance to the Gulf with a bearing of 180' to the abandoned wooden
                    structure. There were very few oysters at Station 1. Station 2 was collected
                    from an exposed reef on the west bank of the bayou on the second bend
                    approximately 0.5 km upstream from the Gulf. Bearings to locate the site
                    were 130' to abandoned structure; 320' to deer stand. Station 3 was collected
                    from an exposed reef 60 m north of Station 2.

              Year II - In Year 11, the mouth of Joseph Bayou had been dredged a few days
                    prior to collecting. This reduced the number of oysters present but the
                    station locations were the same.

              Years III, IV, & V - Oysters were collected from the same stations as described
                    in Year I.

              Years VI, VII, & VIII - Stations were located in the same areas as previous
                    years. Oysters were more abundant and larger.

              Year IX - This site was not scheduled for collection this year.

                   MENT COLLECTIONS

              Years I, II, & III - Sediment stations were collocated with each oyster collection
                    site.

              Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                    years.





                                                      243










               &0"LINGA19MOD

                      Oysters - hand
                      Sediment- hand

               WATER DEPTH - intertidal, 0.2 m

               POSSIBLE CONTAMINANTS - Dredging was the only source of contamination
               observed at the site.


               ENVIRONMENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (,Y00)               (0c)

                    YEAR 1                  20.0                 22.0           31 March 1986
                    YEAR 11                   7.0                18.0          10 February 1987
                    YEAR 111                37.0                 13.0          17 December 1987
                    YEAR IV                 33.0                 16.0          8 December 1988
                    YEAR V                  15.0                 15.0          6 December 1989
                    YEAR VI                 25.0                 13.0          7 December 1990
                    YEAR VII                10.0                 12.7          5 December 1991
                    YEAR VIII               10.0                 13.0          11 December 1992


































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                                                                Site #21 (JHJH), J. Harbor Bayou Joseph Harbor Bayou (from chart 11345).







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                           UP









              SITE NUMBER - 22                             DESIGNATOR - VBSP

              SITE - SOUTHWEST PASS, VERMILION BAY, LA

              NONUNAL SrIE CENTER - 29034.60'N             92002.75V

              1A)CATED ON NOS CHART # - 11345) 11349

              SITE ACCESS - Departure point and boat launch is at Cypremort Point State
              Park, and run time across Vermilion Bay is about I hour. Shoaling is very steep
              and treacherous through Southwest Pass, especially on the west side of the
              channel opposite Marsh Island. Keep in the channel until seaward of channel
              marker 3 or well into Vermilion Bay.

              SITE DESCRIPTION - The site was located adjacent to the channel outside of
              lighthouse point in lease 25918. Oysters are located at the mouth of Southwest
              Pass. Oyster reefs and private leases are ubiquitous in the area, but oysters are
              not necessarily in the same place each year. There were no significant
              geographic differences recognized in the oyster stations for this site.

              OYSTER COLLECTIONS

              Year I - Oysters were collected due east of channel marker 3 in Year I.

              Years II & III - In Year II, only dead shells were found at the Year I site, so in
                    Years II and III, oysters were purchased from a commercial dredging boat
                    operating on private leases on the east side of channel marker 3. Therefore,
                    no distinct stations were recognized.

              Year IV - The site was the same as in Year II and III. Oysters were purchased
                    from a commercial dredger operating to the east of the channel entrance;
                    east of channel marker 3 and south of channel marker 5. No distinct
                    stations were delineated.

              Year V - Oysters were purchased from a commercial lugger working 200 m east
                    of channel marker 3. Stations were not distinguished.

              Year VI - Oysters were purchased from a commercial lugger working 200 m west
                    of channel marker 3 (29'34.89'N, 92'03.14'W). Stations were not separated.

              Year VII - Oysters were dredged from the edge of a private lease approximately
                    200 to 300 m east of channel marker 3. Large oysters were not abundant but
                    many small oysters were present. All three stations were located along a
                    north-south line covering 150 m.

              Year  VIII - Oysters were collected by dredge at the same locations sampled in
                    Year VII.






                                                     249









                Year IX - Oysters were dredged along the edge of a private lease about 220 m west
                       of channel marker #3. Very few oysters were present, and all three
                       stations were collected along a 200 m north-south line. The stations were
                       not seperated.

                   )EMENTCOLLECTIONS

                Years I, H, & III - The mud was approximately 100 m from shore. Sediments at
                       all three stations were 1-2 cm of fine-grained loose brown silt over gray clay.
                       For all years, fine-grain sediments were located northwest from channel
                       marker 3, less than 100 m from shore, and -0.5 mile from the site of the
                       oyster collections. Station 1 was 60 m offshore on a 1900 bearing from the
                       tank on shore; 185' to pair of rusty tanks; 87' to channel marker green 3.
                       Station 2 was 50 m from shore on a line from channel marker 1 to the
                       tanks. Landmarks and bearing were 286' to 4 tanks; 190" to oyster
                       sampling site. Station 3 was on a line from channel marker 3 to the tanks
                       -100 m from the shore.

                Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                       years.

                SAMPLINGMETHOD

                       Oysters - dredge
                       Sediment - box core

                WATER DEPM - subtidal, 1.5 to 2 m

                POSSIUBLE CONTAMINANTS - No point sources were identified other than oil
                wells in Vermilion Bay.

                ENVIRONAWIWAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (0/00)                (0c)

                     YEAR 1                  8.0                   -            31 March 1986
                     YEARII                  8.0                  11.0          10 February 1987
                     YEAR 111               22.0                  10.0          28 January 1988
                     YEAR IV                10.0                   -            6 January 1989
                     YEAR V                 10.0                  11.0          6 January 1990
                     YEAR VI                10.0                  12.0          8 January 1991
                     YEAR VII                5.0                  16.0          8 January 1992
                     YEAR VIII               5.0                  18.0          5 January 1993
                     YEAR IEK                3.0                  8.0           11 January 1994






                                                           250

















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              SrIE NUMBER - 23                             DESIGNATOR - ECSP

              SITE - SOUTH POINT, EAST COTE BLANCHE, LA

              NONONAL SITE CENTER - 29028.50'N             91048.OOV

              LOCATED ON NOS CHART           11345,11351

              SITE DESCRU?TION - The site that was originally examined is a dead oyster reef
              at the mouth of Oyster Bayou (through Marsh Island) adjacent to spartina marsh
              along the shoreline. No oysters were sampled/available; sediments were
              sampled. This site was located on the southeast side of Marsh Island. With the
              help of the Louisiana Fish and Wildlife Department, we found the very old shell
              rubble reefs but no living oysters of any size. Oystermen of the area reported no
              living oysters were known to be in the entire area of East Cote Blanche. The site
              was deleted from study as no alternate site in the geographic area was available.

                                     Salinity        Temperature       Dates Sampled
                                       (%0)               (OC)

                   YEAR 1               4.0               22.0           1 April 1986









              SrIE NUMBER - 24                               DESIGNATOR - ABOB

              SITE - OYSTER BAYOU, ATCHAFALAYA BAY, LA

              NONEINAL SITE CENTER - 29014.40'N              91008.10'W

              LOCATED ON NOS CELART            11351

              -SITE ACCESS - The only access to the site is by a 1 hour boat ride. The only
              launch ramp is at Dularge Sporting Goods. Proceed south on Bayou Dularge,
              across Caillou Lake into the open Gulf and then west along the beach front to
              Oyster Bayou. A more circuitous route is possible through Lake Merchant,
              winding through the bayou to Four Leagues Bay, then south to Oyster Bayou.
              However, the bayou is shallow and should not be attempted with boat draft of 2 feet
              or more or at low tide.

              SITE DESCRIPTION - The sampling stations were located throughout the length
              of the Oyster Bayou which joined Four League Bay with the Gulf of Mexico on the
              south. Oysters were extremely abundant and were obtained either with a dredge
              or collected by hand along the shore. However, the entire area was leased by Mr.
              Wilson Voisin. Oysters could be obtained only with his permission or purchased
              by the sack from his boats when they were oystering.

              OYSTER COLLECTIONS - In all years when oysters were collected by hand,
              dredge or purchased, the sampling stations were: Station 1 was at the mouth of
              the bayou, along the west bank; Station 2 on a reef paralleling the west side of the
              bayou -300 m north of the shrimp drying facility; and Station 3 was a large reef in
              Four Leag-ue Bay at the north end of Oyster Bayou. Landmarks included the
              channel entrance marker, an abandoned shrimp drying facility, and the ends of
              the bayou where it entered the bay and Gulf.

              Year I - Oysters were collected by hand.

              Year II - Oyster were purchased from a commercial oyster dredge.

              Year III - Oyster were collected by dredge.

              Year IV - The stations sampled in Year IV were the same as were sampled for all
                     previous years. Oysters were dredged at Station 1 and collected by hand
                     near the shore at Stations 2 and 3.

              Year V - Oysters were purchased from Mr. Wilson Voisin. They were dredged by
                     a lugger working on the north end of the bayou between Station 1 and 2. No
                     stations were designated. A recent freeze had killed all of the oysters along
                     the shoreline and to a depth of 1 m due to extreme low tides.

              Year   VI - Oysters were collected this year since the area was closed due to
                     excessive freshwater. Station 1 was dredged at the north end of Oyster
                     Bayou on the west side of the channel. Station 2 was collected by hand from


                                                       257








                      a reef on the west side of the channel across from the three buildings-
                      Station 3 was also on the west bank approximately 0.5 mi north of the
                      mouth of Oyster Bayou and 300 m south of the shrimp drying facility ruins.

               Year VII - Oyster collection Stations I and 2 were located at the same sites as last
                      year. Station 3 was just to the south of station 2. Oysters were abundant
                      this year.

               Year VIII - Oysters were collected at the same locations sampled in Year VII.

               Year IX - The site was not scheduled for collection this year.

                   04ENTCOLLEMONS

               Years 1, 11, & III - Sediment Station 1 was located in a small cove on the west
                      bank at the mouth of Oyster Bayou. Station 2 was located on the east side
                      shoreline 0.5 miles north of Station I and was 300 m south of the shrimp
                      dryer at a fringe marsh along the east shore. Station 3 was located north of
                      Station 2 on the east shore 0.5 miles south of an abandoned shrimp drying
                      plant. Station 3 was at the southernmost piling of the old dock at the three
                      wooden buildings with rusty tin roofs. Sediments were variable but were
                      generally soft gray-black mud.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.

               SAMPLINGAIETHOD

                      Oyster - hand, dredge or purchase
                      Sediment - box core

               WATER DEPM - intertidal to subtidal, 0 to 2 m

               POSSIBLE CONTANIINANTS - No sources were observed.


               ENVIRONDUM"4TAL DATA

                                         Salinity          Temperature          Dates Sampled
                                           (1700)                (OC)

                    YEAR 1                 11.0                  23.0           29 March 1986
                    YEAR 11                  6.0                 16.0          11 February 1987
                    YEAR 111               11.0                  12.0          29 January 1988
                    YEAR IV                13.0                  21.0           7 January 1989
                    YEAR V                 21.0                  13.0           7 January 1990
                    YEAR VI                18.7                  14.2           9 January 1991
                    YEAR VII                 8.0                 16.0           9 January 1992
                    YEAR VIII                5.0                 17.0           6 January 1993




                                                           258














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               SITE NUMBER - 25                              DESIGNATOR - CLCL

               SITE - CAILLOU LAKE, CAILLOU LAIKE, LA

               NOMI]NAL SITE CENTER - 29015.25'N             90055.80'W

               LOCATED ON NOS CHART            11356

               SITE ACCESS -     'A boat launch is at Bayou Dularge Sporting Goods at the end of
               LA Highway 315. Take Bayou Dularge southwest to the channel separating Lake
               Caillou and Lake Merchant and turn south toward the Fish and Wildlife Camp on
               the island. Run time to the site is 0.5 hrs.

               SITE DESCRIPTION - The site was located in Lake Caillou on a submerged reef
               running from channel marker 16 to Fish and Wildlife Camp. The reef was
               usually closed to oystering, and it was necessary to notify the F&WL camp prior to
               sampling. Dredging or tonging was required.

               OYSTM COLLECTIONS

               Year I - Oysters were patchy on the bottom running on a line from the channel
                     marker to the camp, and could only be located by feeling the bottom with a
                     bamboo pole. No station distinctions were made at this open water subtidal
                     site because of the sparsity of oysters.

               Year II - Oysters were dredged from the same location as the first year.

               Year Iff - Year III oysters were abundant and were easily dredged.

               Year IV - In Year IV, the oysters were dredged from approximately the same
                     location as was sampled in all previous years. The dredging was done
                     between channel markers 14 and 16, in front of the F&WL camp, and about
                     150 m east.

               Year V - Oysters were dredged from the same general locations as previously
                     sampled. Station 1 oysters were collected between channel marker 16 and
                     the F&WL camp. Stations 2 and 3 were collected 200 m south of channel
                     marker 16 where the reef runs north-south. The bottom was soft mud and
                     scattered shell.

               Year VI - All three oyster stations were dredged from the same area as previously
                     sampled (29'15.42'N, 90'55.91'W). Calliou Lake was opened two weeks for
                     commercial oysters just prior to our sampling. Because of this oysters were
                     not abundant.

               Years VII & VIII - Oysters were collected in the same general locality. Several
                     tows of the dredge were made approximately 50 to 100 m east of channel
                     marker 14 on line to the F&WL camp. Oysters were abundant but distinct
                     stations were not recognized due to the limited size of the reef.



                                                       263









               Year IX - Oysters were dredged from a small reef to the east of the channel
                      marker #14, in a line with the Fish and Wildlife camp (29'15.12'N,
                      90'55.85'W). Oysters were abundant but distinct stations were not
                      recognised due to the limited size of the reef.

                    GEMW COLLECTIONS

               Years I, II, & III - Sediments were located to the north of the oyster site, along the
                      shoreline which separated Bayou DuLarge from Lake Caillou. Sediment
                      Station 1 (in all years) was located at the base of channel marker 18. Station
                      2 was located at the mouth of a small cove 300 m to the east. Station 3 was
                      located -100 m from the shore at a large cove which was due north of the
                      FV,TL Camp. Sediments were variable, Stations 1 and 2 were dark gray silty
                      mud with a thin brown top layer. Station 3 was stiff gray clay with a coarse
                      sandy mud layer on top.

               Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                      years.

               SAMPLINGMETUOD

                      Oysters - dredge
                      Sediment - box core


               WATER DEPTH - 2 m

               POSSIBLE CONTAMINANTS - Possible sources of contamination in the vicinity
               included numerous camp houses without sewer systems along Bayou DuLarge.

               ENVIRONMENTAL DATA

                                          Salinity          Temperature          Dates Sampled
                                           (%0)                  (OC)

                    YEAR 1                 14.0                  23.0            29 March 1986
                    YEAR 11                  5.0                 17.0            11 February 1987
                    YEAR 111               15.0                  13.0            29 January 1988
                    YEAR IV                22.0                  22.0            7 January 1989
                    YEAR V                 14.0                  14.0            7 January 1990
                    YEAR VI                14.0                  14.0            9 January 1991
                    YEAR VII                 9.0                 17.0            9 January 1992
                    YEAR VIII                8.0                 18.0            6 January 1993
                    YEAR IX                  5.0                 10.0            12 January 1994









                                                           264










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                                                                                 Site #25 (CLCL), Caiflou Lake Caillou Lake.








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                SrIE NUMBER - 26                              DESIGNATOR - TBLB

                SITE - 1AKE BARRE, TERREBONNE BAY, LA

                NONEINAL SITE CENTER - 29015.60'N              90035.70"W

                LOCA= ON NOS CHART # - 11357

                SITE ACCESS - Access to the site is by boat launched in Cocodrie at the Coco
                Marina and then north on Bayou Petit Caillou. Turn right (east) into Lapeyrouse
                Canal and proceed to Bayou Terrebonne, turn south and proceed to the third cut
                into Bay la Fleur. Run time to the site is approximately 0.5 hrs.

                SITE DESCRIPTION - The three stations were located along Bayou Terrebonne.
                In Year I, Stations 1 and 2 were on the west side of the channel and Station 3 was
                on the east side. In Year II and following years, Station 1 and 3 were reversed
                and Station 2 remained unchanged.

                OYSTERCOLLECTIONS

                Year I - Station 1 was off Bayou Terrebonne in a small bay on the west side of the
                      bayou about 0.5 miles north of Lake Barre. Landmarks and bearing were
                      265' to LUMCON tower; 163' to green channel marker 3, and 106' to the
                      Montegert water tower. Station 2 was located upstream at the mouth of
                      bayou in grass near an old fishing camp on Bayou Terrebonne at the first
                      cut to the west into Bayou Lucien. Bearings were 258' to LUMCON; 116' to
                      the green channel marker 1. Oysters and sediments were taken near the
                      remains of a galvanized corrugated iron shed and wood dock in intertidal
                      waters on the northwest shore. Station 3 was farther north on Bayou
                      Terrebonne at the next cut (Lake Barre pass) to the east which was the first
                      cut to the east north of Seabreeze Pass. This cut connected to Bay la Fleur
                      on the east side of Terrebonne Bayou. Oysters were from an intertidal reef
                      on southwest shore and sediments from subtidal waters. The reef was
                      exposed at low tide. Oysters were abundant at all three sample areas.

                Years II, III, IV, V, & VI - Stations 1 and 3 were reversed these years.

                Years VII & VIII - Stations were located in the same sites sampled the previous
                      five years. Station 1 on the north, Station 2 in the middle and Station 3 was
                      on the south end of the bayou.

                Year LX - The site was not scheduled for sampling this year.

                SMEMENT COLLECTIONS

                Years I, II, & III - Sediment collections were collocated with each of the oyster
                      stations.






                                                        269








             Years IV, V, VT VII, VIII, & IX - No sediments were sampled during these
                    years.

             SAMPLINGMETHOD

                    Oysters - hand
                    Sediment - hand

             WATER DEPTH - intertidal, 0. 1 - 0.5 m

             POSSIBLE CONTAMINANTS - Contamination factors included marine traffic
             and the fish camp ruins at Station 2.

             ENVIRONMENTAL DATA

                                        Salinity          Temperature          Dates Sampled
                                          (1700)               (OC)

                  YEAR 1                  17.6                 20.0            29 March 1986
                  YEAR 11                 17.6                 20.0          11 February 1987
                  YEAR 111                18.0                 11.0           29 January 1988
                  YEAR IV                 25.0                 19.0            7 January 1989
                  YEAR V                  18.3                 16.0            6 January 1990
                  YEAR VI                 19.0                 14.3            8 January 1991
                  YEAR VII                15.0                 16.0            9 January 1992
                  YEAR VIII               10.0                 18.0            6 January 1993

























                                                         270















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              SITE NUMBER - 27                               DESIGNATOR - TBLF

              SITE - LAKE FELICITY, TERREBONNE BAY, LA

              NONONAL SITE CENTER - 29015.80'N               90024.40V

              LOCATED ON NOS CHART # - 11357

              SITE ACCESS - The launch/departure point is Coco Marina at Cocodrie or the
              boat ramp at LUMCON, and the run time is -1.5 hours to the site by boat. A deep
              draft outboard must enter and cross Terrebonne Bay to Lake Felicity. A shallow
              draft outboard can shortcut up the bayou to enter and cross Lake Barre (see
              directions for TBLB). The site is located at an abandoned and dilapidated fish
              camp on the southeast side of Lake Felicity, near Bayou Point Au Chien.
              Landforms in the area bear little resemblance to any maps or charts of the area
              and navigation to the site by reference to shoreline features as depicted in charts
              is nearly impossible.

              SITE DESCRIPTION - The oysters were intertidal and interspersed in the grass
              beds on the small islands surrounding the fish camp. The only landmark in
              locating the oyster stations was the fish camp structure, which burned down in
              Year III.


              OYSTER COLLECMONS

              Years  I, II, & III - Station 1 was located at southeast corner of bay, south of
                     channel marker 5 for Felicity Bayou at an abandoned fish camp. Station 1
                     was located on the island (grass covered flat) at the abandoned fish camp.
                     Station 2 was a subtidal Spartina bed across the channel on the island
                     bearing 162' from the fish camp. Station 3 was a subtidal grass bed west of
                     the fish camp on the island bearing 240' from the fish camp.

              Years IV, & V - Prior to the Year IV sampling, the fish camp had burned to the
                     ground. In Year IV, Stations I and 3 were in the same locations but Station
                     2 was moved due to the lack of oysters. The new Station 2 was located
                     approximately 200 m northwest (320 bearing from Station 1) of the dock at
                     the fish camp. Station 2 is a small reef at the edge of the Spartina marsh.

              Years VI, VII, VIII, & IX - Oysters were collected at the same locations as in
                     Years IV & V.


              SEDDEEW COLLECTIONS

              Years I, II, & III - Sediments are silty mud and collocated with the oysters.
                     Sediments were taken from the subtidal waters adjacent to each of the
                     oyster stations.

              Years IV, V, VI, VII, VIII, & IX - No sediments were sampled during these
                     years.



                                                      Z75










                SAMPLIN'G METHODS

                        Oysters - hand
                        Sediment - hand

                WATER DEPTH - intertidal, 0 to 0.5 m

                POSSIBLE CONTAMINANTS - There were no obvious sources of contamination
                .in the vicinity except for the very large producing oil and gas field in Terrebonne
                Bay between Lake Barre and Lake Felicity.

                ENVIRONMENTAL DATA

                                             Salinity            Temperature           Dates Sampled
                                                (%00)                  (0c)

                      YEAR 1                    19.0                   21.0            28 March 1986
                      YEAR 11                   16.0                   20.0            11 February 1987
                      YEAR 111                  27.0                   15.0            29 January 1988
                      YEAR IV                   26.0                   22.0            7 January 1989
                      YEAR V                    21.0                   15.0            7 January 1990
                      YEAR VI                   26.0                   14.0            9 January 1991
                      YEAR VII                  20.0                   16.0            9 January 1992
                      YEAR VIII                 20.0                   16.0            6 January 1993
                      YEAR EK                   15.0                   15.0            11 January 1994



























                                                                276













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                                                                               Site #27 (TBLF), Terrebonne Bay Lake Felicity (from chart 11357).



































































                                                                                                                                                                                                                                                              141




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               SITE NUMBER - 28                               DESIGNATOR - BBSD
               SITE - BAYOU ST. DENIS, BARATARIA BAY, LA

               NONONAL SITE CENTER - 29024.18'N               89059.75W

               LOCATED ON NOS CHART            11358

               SITE ACCESS - The site is accessed by boat, launching at the Happy Jack Marina
               in Point Sulfur or the Cheramines Marina in Grand Isle. From Grand Isle
               proceed north up the Barataria Waterway to green channel marker 31. Islands
               and landforms have changed since the chart was published, and the land forms
               provide little reference for determining position. Run time to the site is
               approximately 1 hr.

               SITE DESCRIPTION - This site was located in Bayou Cholas on the west side of
               Barataria Bay and the Barataria Waterway. Oysters have been collected in the bay
               area and along the shoreline of the small island on the north side of the bay.

               OYSTER COLLECTIONS

               Years I & II - Oysters in Years I and II were taken from intertidal mud and sand
                     bottom on the west side of island west of green channel marker 31. The
                     oysters found were very old and few in numbers. Year I oysters were
                     purchased from a commercial lugger working on the reefs in the area.
                     Station I was on the south part of the island, at a bearing of 360' to channel
                     marker 9. Station 2 was 120 m north of Station 1. Landmark bearings were
                     121' to channel marker 9; 20' to channel marker 11. Station 3 was 100 m
                     north of Station 2. Bearing were 65' to pipeline platform and 285' to
                     channel marker 9.

               Year  III - Year III stations were on a submerged reef west of Barataria
                     Waterway between channel marker 30 and 33, near several small islands to
                     the west in Bayou Cholas. Station 1 was on a bearing of 30' to green marker
                     13 and 105' to marker 9. Station 2 was 50 m to the south, and Station 3 was
                     another 50 m south.

               Year  IV - Due to the lack of oysters at the stations sampled in Year III, the
                     sample location was moved approximately 500 m to the east. Year IV
                     stations were located on the island directly west of channel marker 33.
                     Station 1 was on the east end of the island (nearest the channel) and
                     extended along the south facing shoreline for 50 m. Station 2 was in the
                     middle part of the island and was 50 m due west of Station 1. Station 3 was
                     on the west end of the island next to Station 2.

               Year  V - Stations collected this year were those described in Year IV. Oysters
                     were scarce. There were numerous dead oysters and fish due to the recent
                     hard freeze.





                                                       281









               Year VI - There were no oysters on the island this year so the site was moved back
                      to the submerged reef Stations sampled in Year III were resampled this
                      year.

               Year VII - There were no live oysters found in this part of the bay.

               Year VIII - Oysters were present again this year, but were scarce and small.
                      Stations sampled in Year III were resampled this year.

               Year LX - The site was not scheduled for sampling this year.

                  )DfflE-NT COLLEMONS

               Years L& II - Sediments were taken from subtidal areas west of channel marker
                      26.

               Year III - Sediments were taken adjacent to the reef 30-40 m east of each station.

               Year IV - Sediments were collected 100 to 150 m south of each oyster station in 1 to
                      1.5 m of water.

               Years V, VT VA VIII, & IX - Sediments were not sampled during these years.

               &AMPUNG METHOD

                      Oysters - commercial dredge, tongs, and hand
                      Sediments - box core

               WATER DEPTH - intertidal or subtidal, 0.3 to 3 meters

               POSSEBLE CONTANUNANTS - Contamination factors included marine traffic,
                      dredging, and a compressor station.

               ENVI[RONbEENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (1700)               (0c)

                    YEAR 1                 19.0                 19.0           27 March 1986
                    YEAR 11                19.0                 19.0          13 February 1987
                    YEAR 111               12.0                 11.0          28 January 1988
                    YEAR IV                19.0                 18.0           6 January 1989
                    YEAR V                 16.0                 16.0           6 January 1990
                    YEAR VI                  7.7                15.0           8 January 1991
                    YEAR VII               20.0                 16.0           8 January 1992
                    YEAR VIII              10.0                 19.0           5 January 1993






                                                          282















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                                                                                                                                        Site #28 (BBSD), Barataria Bay Bayou St. Denis (from chart 11358).




















































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               SrIE NUMBER - 29                                 DESIGNATOR - BBMB

               SITE - MIDDLE BANK BARATARIA BAY, LA

               NONEINAL SITE CENTER - 29016.55'N                89056.53V

               LIDCATED ON NOS CHART # - 11358, 11365

               SITE ACCESS - During the first two years of the study, the boat was launched at
               the High Point Marina, Point Sulfur. The run from this point is at least 2 hours.
               The site is now accessed by boat launched at Cheramines Marina in Grand Isle.
               From the marina, proceed east across Barataria Pass to the Louisiana Fish and
               Wildlife Camp on the northwestern tip of the westernmost island of the Grand
               Terre Islands. Run time is less than 10 minutes.

               SITE DESCRIPTION - This site was located in Spartina alterniflora marsh along
               the channel going to the Grand Terre F&WL station on the east side of the
               channel to F&WL camp harbor. The site was only 10 minutes from Grand Isle.
               Oysters and sediments during Years I, II and III were collected from intertidal
               waters inside the Fish and Wildlife boat basin. Oysters were found scattered on
               the mud and sand bottom. There were very few oysters during Year II. There
               was a reported die-off after the first year's collection.

               OYSTERCOLLECTIONS

               Years I, II, III, IV, V, VI, VII, & VIII - Station locations for oyster collections
                      were: Station 1 - located at the dock; Station 2 - 50 m to the north; Station 3 -
                      70 m north of Station 2. Station locations were the same for all eight years.

               Year IX - The site was not scheduled for sampling this year.

               SEDIMENT COLLECTIONS

               Years I, II, & III - Sediments were collected along the channel adjacent to each of
                      the oyster stations.

               Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                      years.

               SAMPLINGMETHOD

                      Oysters - hand
                      Sediment - hand

               WATER DEPTH - intertidal, 0 to 0.5 meters

               POSSIBLE CONTAMINANTS - Possible contamination would be from Fish and
               Wildlife bottom-fouling paint and possibly from pesticides.



                                                         287










               ENVIRONADMAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                  (.C)

                    YEAR 1                 22.0                  24.0          27 March 1986
                    YEAR II                  -                   22.0         12 February 1987
                    YEAR 111               23.0                  11.0          28 January 1988
                    YEAR IV                29.0                  21.0          6 January 1989
                    YEAR V                 30.0                  18.0          6 January 1990
                    YEAR VI                23.0                  16.0          8 January 1991
                    YEAR VII               27.0                  18.0          8 January 1992
                    YEAR VIII              20.0                  19.0          5 January 1993




































                                                           288







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              SITE NUMBER - 61                              DESIGNATOR - BBTB

              SITE - TURTLE BAY, BARATARIA BAY, LA

              NONIINAL SITE CENTER - 29"30.67N              90005.00'W

              LOCATED ON NOS CFLART           11352

              -SITE ACCESS - Access to this collection site is at the marina at the end of LA
              Highway 45. By boat proceed north on Barataria Waterway and turn left into
              Bayou Rigolettes. Follow Bayou Rigolettes south of Harvey Cutoff and then south
              to Turtle Bay. In Turtle Bay proceed southeast for approximately 1.2 miles past
              the mouth of Long Bay to the mouth of Bayou St. Denis past several small islands
              en route.

              SITE DESCRIPTION - The site, established in Year III, was located on the south
              side of the entrance to Bayou St. Denis from Little Lake at a row of red and white
              poles which parallel the shoreline to the south. Oysters were not abundant;
              therefore distinct stations were not established. Oysters were dredged from a
              black muddy bottom which contained numerous dead shells. Landmarks to
              locate the site were: 110' to a small treed island and 30' to a green camp house
              and silver storage tank.

              OYSTER COLLECTIONS

              Year III - Station 1 was at the north end of the island 50 m from shore. Station 2
                     was 20 m offshore, opposite the oyster dredge site. Station 3 was at the
                     south end of the dredge site in a small cove 20 m from shore.

              Years IV, V, VI, VII, & VIII - This site was not sampled during these         years.

              Year IX - The site was revisited this year and no live oysters were found within a
                     half-mile radius. An alternate site - MBCP (Cedar Point Reef, Mobile Bay)
                     was substituted.

                  3IM[ENTCOLLECTIONS

              Year III - Sediments were taken along the shoreline to the south. Sediments
                     were black fine silty mud with detrital matter and an obvious H2S odor.

              Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                     years.

              SAMPLING Affi'THODS

                     Oysters - dredge
                     Sediment - box core

              WATER DEPTH - subtidal, 2 to 3 meters



                                                      293








            POSSIBLE CONTAMINANTS - No point sources were evident, however there
            were several petroleum facilities in the general area.

            ENVIRONMENTAL DATA

                                 Salinity      Temperature      Dates Sampled
                                   (0/00)           (0c)

                YEAR 111           12.0             15.0       30 January 1988
                YEAR IEK           53               12.0       12 January 1994



































                                               294












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               SrIE NUMBER - 65                                DESIGNATOR - MRTP

               SITE - TIGER PASS, MISSISSIPPI RIVER, LA

               NONEINAL SITE CENTER - 29008.69'N               89025.67W

               LOCATED ON NOS CHART             11361

               SITE ACCESS - Access to the site is by boat. From the boat launch at the Venice
               Marina proceed down Tiger Pass; this takes 0.5 to 1 hour depending on weather
               conditions. It is best not to go to the site in foggy weather. The mouth of Tiger
               Pass is bordered by a jetty on both sides; proceed past the jetty on the north side to
               channel marker 7 then turn north and go back to the shoreline. The site is
               directly north of the jetty and channel marker 9.

               SITE DESCRIPTION - This site, established in Year III, was located on the west
               side of the Mississippi River delta near the mouth of Tiger Pass. The site was on
               the southwest shoreline of a small smooth cordgrass island. The site was located
               near a telephone pole at the edge of the marsh.

               OYSTER COLLECTIONS

               Year III - Individual stations were contiguous along the shoreline. Station 1 was
                     along the shoreline just north of where the Tenneco pipeline canal ran into
                     the bay on the east and southeast from the pole at Station 2. Station 2 was
                     the next -100 m of shoreline to the north across from the telephone pole
                     with two bolts. The pole was approximately 20 m from the shoreline.
                     Station 3 was northwest from the pole up to where the canal forms the
                     northwest boundary of the marsh island. Oysters at all three sites were
                     found lying in the edge of the marsh in the smooth cordgrass marsh.

               Year IV - The same three- stations were again sampled, except that Station 3 was
                     combined with Station 2 because of the scarcity of oysters in the Station 3
                     location. Station 2 oysters were larger and more abundant than in the
                     initial sampling in Year III.

               Year  V - There were very few oysters present this year. Due to the scarcity of
                     oysters, Stations 1 and 2 were not distinguished. All the oysters in the area
                     were collected and split into two groups. Station 3 was in the same location
                     as previous years. The number of oysters has decreased each year and it
                     may be necessary to move this site.

               Year VI - The same site was collected this year. Oysters were almost depleted
                     from this area and only a partial set of oyster samples were collected this
                     year. Additional sites in the immediate vicinity were examined but no
                     oysters were present. There was no oyster reproduction occurring at this
                     site and there were no oysters remaining to be collected, therefore a new
                     site farther to the north should be established.





                                                        299









               Year VII - The same site was collected this year. Oysters were not abundant thus
                     .distinct sites were not recognized.

               Year VIII - The same stations sampled in Year V were resampled this year;
                     however, dinstinct stations were not recognized since oysters were not
                     abundant.

               Year IX - The site was not scheduled to be visited this year.

                   IIMENTCOLLEMONS

               Year III - Sediments were collected offshore of the oyster stations. Sediments
                     were composed of fine silty muds taken 20-50 m offshore from the oyster
                     stations. Station 1 was at the southeast point of the marsh island, Station 2
                     was 20 m offshore from the oysters. Station 3 was northwest across the
                     channel from the oysters.

               Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                     years.

               SAMPLINGMETHOD

                     Oysters - hand
                     Sediments - box core

               WATER DEPTH - intertidal, 0 to 0.1 meters

               POSSEBLE CONTAMINANTS - No known sources of contamination were visible.


               ENVEEMNAUKWAL DATA

                                         Salinity          Temperature         Dates Sampled
                                            (1700)              (0c)

                   YEAR 111                 30.0                13.0           31 January 1988
                   YEAR IV                  23.0                19.0           8 January 1989
                   YEAR V                   30.0                15.0           8 January 1990
                   YEAR VI                  10.0                14.0           11 January 1991
                   YEAR VII                 >0.5                12.0           10 January 1992
                   YEAR VIII                2.0                 13.0           7 January 1993















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              SrIE NUMIBER - 64                              DESIGNATOR - MRPL

              SITE - PASS A LOUTRE, MISSISSIPPI RIVER, LA

              NONGNAL SITE CENTER - 29004.87'N               89005.53V

              LOCATED ON NOS CEURT # - 11361

              SITE ACCESS - The departure point for this site established in Year III is from
              the Venice Marina. From the boat basin, head upstream to the main fork of the
              Mississippi River and proceed downstream to Head of Passes. At this point, take
              the east fork holding to the south shore to avoid the large sandbar at the entrance
              to Pass a Loutre. Proceed east to the first cut to the south and proceed to the
              Louisiana Fish and Wildlife Headquarters which is located south of the entrance
              to Johnson Pass. The Louisiana Fish and Wildlife personnel provided guidance to
              the site the first year. Proceed back upstream to Loomis Pass, turn to the
              southeast into the pass and proceed downstream entering Cognevich Pass and on
              into the Gulf of Mexico. At this point, turn north to the mouth of Redfish Bay. No
              attempt should be made to travel to this site except in calm weather. In Year IX,
              access was gained to Redfish Bay via Pass a Loutre, down SE Pass to the first oil
              tanks (29*07.75'N, 89'05.78W), and then south into Redfish Bay via a narrow reed
              lined channel.

              SITE DESCRIPTION - The site is located at the extreme southeast edge of Redfish
              Bay just north of the last small island. Prominent landmarks are the Port Eads
              Lighthouse (240') at South Pass and the Exxon platform with green tanks (3501).

              OYSTER COLLECTIONS

              Year III - No distinct oyster stations were established at this site. Oysters were
                     located by poling and occur in scattered areas of large clumps of old large
                     oysters. This was an old reef which has silted over with fine sediment.
                     Oysters were collected with tongs and are difficult to break loose from the
                     reef.

              Year IV - Oysters were collected by dredge in the same area that was tonged in
                     Year III. Sampling was difficult because of windy conditions and the
                     overall sparsity of live oyster beds. Dredging yielded barely enough for two
                     replicates. The site was located about 200 m east of the seawardmost pole
                     marking the entrance to Johnson Pass, and on a line to the small barrier
                     island. The only way to find the isolated scatterings of oysters in the soft
                     mud was to randomly dredge or feel the bottom with a bamboo pole. This
                     did not distinguish live and dead reefs. Oysters could not be hand picked
                     from the marsh plants on the nearby islands because the mud was far too
                     soft to walk on and the water was too shallow to approach by boat.

              Year   V - Oysters were again dredged from along the north side of the last
                     Phragmites island on line with Port Eads lighthouse and the green oil
                     tanks (29'04.30'N, 89*04.60'W). Oysters were found primarily on roots and


                                                      305








                   old crabs pots and were very scarce. It required 2 hours of dredging to
                   obtain enough oysters for three replicates. The substrate was a black
                   anoxic mud.

            Year VI - This site was not sampled this year.

            Year VII - The site was moved slightly to the north and west of the Year V site.
                   Oysters were dredged from a linear submerged reef west of the small
                   Phragmites island. The site was approximately 500 m due south of the
                   green oil tanks located near the north end of Redfish Bay. The reef was very
                   narrow and difficult to locate.

            Year VIII - Oysters were dredged from the south end of the linear reef described
                   in Year VII. Distinct stations were not recognized since extensive
                   dredging was required to obtain an adequate sample.

            Year IX - A very small patch of live oysters was found on the extreme end of the
                   SE promontory on Redfish Bay, just north of the southernmost island. The
                   oysters were dredged 5 to 10 m out from the shore in 1 m of water. Seperate
                   stations were not distinguished due to the size of the reef. The rest of the
                   bay was devoid of any live oysters.

                 EMENT COLLECTIONS

            Year III - Three sediment stations were established on the reef -100 m apart.
                   Fine grained silty sediment were mixed with shell.

            Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                   years.

            SAMPLING MEETHODS

                   Oyster - tongs or dredge
                   Sediment - box core

            WATER DEPTH - subtidal, 1 to 5 meters

            POSSIBLE CONTAMINANTS - The only point sources were the nearby oil
            productions facilities in the upper part of Redfish Bay.









               ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%o)                  (0c)

                    YEAR III                 -                   13.0         26 February 1988
                    YEAR IV                 2.0                  15.0          9 January 1989
                    YEAR V                  9.0                  13.0          9 January 1990
                    YEAR VII                >0.5                 13.0          10 January 1992
                    YEAR VIII               5.0                  13.0          7 January 1993
                    YEAR IX                 2.0                  8.0           14 January 1994







































                                                          307






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                                                                                                                      Miami










               SITE NUMIBER - 30                               DESIGNATOR - BSBG

               SITE - BAY GARDENE, BRETON SOUND, LA

               NOXINAL SITE CEN R - 29035.90'N                 89037.25V


               LOCATED ON NOS CHART # - 11364

               SITE ACCESS - The site is accessed by boat launched at Pointe a la Hache from
               the Chevron Marina. Proceed from the marina southeast down the Back Levee
               Canal to Lower Grand Bayou, then east along the bayou to Battle Ground Bay. In
               Battle Ground Bay go generally east paralleling the shore to Tripple Pass. Then
               go northeast and northwest to Pintail Point in Bay Gardene. At Pintail Point
               proceed east-northeast to Bayou Lost to the Louisiana Fish and Wildlife Camp.
               The site is along the south shore of the western end of Bayou Lost, west of the Fish
               and Wildlife Camp. Approximate run time to the site is 0.75 hours.

               SITE DESCRIPTION - This site was located on small unnamed islands between
               Bayou Lost and Lonesome Island. Oysters'were abundant. No visible
               contamination factors were observed.


               OYSTER COLLECTIONS

               Year I - Oysters were collected by dredge off the western tip of the island.
                      Landmarks used to located the position are: 680 to the Fish and Wildlife
                      camp, 129' to white grain elevators, and 293" to the oil terminal.

               Years II & II - In all years, Station 1 was collected on the westernmost tip of the
                      island on the south side of Bayou Lost near Pintail Point in Bay Gardene.
                      Station 2 was 100 m to the east of Station 1. Station 3 was 100 m to the east of
                      Station 2.

               Years  IV, V, VI, VII, & VIII - The stations sampled were the same as in
                      previous years, along the east end of the island at the Fish & Wildlife camp
                      on the south shore of Bayou Lost.

               Year IX - The site was not scheduled for sampling this year.

               SEDIME14T COLLECTIONS

               Years I, II, & III - Sediments were collected by box corer in subtidal waters
                      adjacent to the oyster collection areas.

               Years IV, V, VI, VII, VIII, & LY - Sediments were not sampled during these
                      years.









                                                         312










               SAMPUNGAIEMOD

                      Oysters - hand and dredge
                      Sediment - hand and box core

               WATER DEPTH - intertidal or subtidal, 0. 1 to 3 meters

               POSSIBLE CONTAAIINANTS - No sources observed.

               ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                 (OC)

                    YEAR 1                 15.0                 18.0           26 March 1986
                    YEARII                 15.0                 18.0          14 February 1987
                    YEAR 111               12.0                 17.0          25 February 1988
                    YEAR IV                20.0                 21.0           8 January 1989
                    YEAR V                 10.0                 14.0           8 January 1990
                    YEAR VI                24.0                 17.0          10 January 1991
                    YEAR VII               10.0                 13.0          10 January 1992
                    YEAR VIII              10.0                 16.0           7 January 1993




























                                                          313












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              SHE NUMBER - 31                                DESIGNATOR - BSSI

              SITE - SABLE ISLAND, BRETON SOUND, LA

              NOAHNAL SITE CENTER - 29024.11'N               89029.10W

              LOCATED ON Nos CHART # - 11364

              SITE ACCESS - During the first two years, access to the site was by boat which
              was launched at Point a La Hache Marina. Proceed southeast on Back Levee
              Canal to Fucich Bayou. Fucich Bayou enters California Bay and channel
              markers are followed to California Point. - Avoid the shoals at California Point by
              staying near the platform structure off the point where the telephone lines appear
              to end. Turn south from California point and cross through the oil field to Sable
              Island. Travel time by boat is 1 to 1.5 hours, and the open water crossing at
              California Bay and south to Sable Island requires a calm day and/or a larger
              protected boat. In Years III-V of the study, the boat was launched at the Venice
              Marina. There are several way to reach the site but the easiest is to go up the
              Mississippi River to the Ostrica Canal Locks and then out the private channel
              across Quarantine Bay to Sable Island. Run time to the site is still about an hour.
              In Year VI a new launch site was used at Delta Marine in Empire. It is
              necessary to go through the Empire Lock then down river to the Ostrica Lock, but
              the run time is much shorter than coming up river. In Year EK, the Buras
              Riverside Marins on the west bank of the Mississippi River, was used. This.
              shortened the boat run time to less than 30 minutes, allowing for the Ostrica
              Locks.

              SITE DESCRIPTION - The site surrounded Sable Island. Oyster reefs were
              ubiquitous around the island, but live oysters were found by trial and error. The
              best oyster beds were on the southwest end of the island. In Year IX, there was a
              good reef, clearly marked with PVC poles, on the SW side of Sable Island
              (29024.26'N, 89029.09'W).

              OYSTER COLLECTIONS

              Years I & H - Oysters were found by commercial oystermen on the north side of
                     the island in Year I (29'24.70'N, 89'28.70'W)(with limited success), and
                     were more abundant on the south side in Year II (29'24.20'N, 89028.80'W)
                     (again with a commercial dredger collecting the samples). Landmarks to
                     help locate the area were: Station 1 - 2450 to well nearest north shore; 310'
                     to tanks nearest large platform. Station 2 - 20' to the nearest well on the
                     north shore; 257' to platform with 4 pipes and 3 tanks. Station 3 - 262' to
                     platform with 3 pipes and 3 tanks; 344' to nearest large platform on the
                     north side of the island.

              Year III - In Year III, oysters were hand collected at Station 1 on the northwest
                     shore of Sable Island (29'24.21'N, 89029.10'W), oysters were not abundant.
                     Oysters at Stations 2 and 3 were purchased from commercial fishermen
                     who were operating just offshore.



                                                      318










             Year X - The oysters were collected by dredge in the area between the larger part
                    of Sable Island and the smaller mostly shell island to the north (29*23.94'N,
                    89'29.09'W). Oysters for all three sites were taken from a marked leased
                    area about 200 m west of the well jacket with a red beacon light. Oysters
                    were plentiful on a mud bottom in 1 m of water.

             Year V - Oysters were dredged on the northwest side of the@ island along the edge
                    of the reef approximately 50 m from the island. All stations were obtained
                    along a 500 m area (29'24.32'N, 89'29.05'W). Oysters were not plentiful.

             Year   VI - All three stations were located off of the southwest end of the island
                    (29024.20'N, 89029.04'W). Station 1 was located on the south end and west
                    side of the island. Station 2 was approximately 100 m farther southwest.
                    Station 3 was another 100 m to the southwest of Station 2. Oysters were
                    sparse at Stations 1 and 2 and abundant at Station 3.

             Year VII - Oysters were collected near the same stations described for Year VI,
                    but the stations were shifted slightly to. the southwest. Oysters were
                    moderately abundant with numerous mussels attached.

             Year VIII - Oysters were collected at the station described for Year VII.
                    Mussels were very numerous, covering most of the oyster shell.

             Year IX - Oysters were abundant and easily dredged from the same station areas
                    as in Year VIII. Numerous small mussels covered most of the oysters.

                 IDIENTCOUECTIONS

             Years I, H, & III - Sediments were available nearshore on the south side. In all
                    years, sediments were collected at three stations -50 m apart on the south
                    side of the island. Sediments were gray-brown silt over dark black mud.

             Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                    years.

             SAMPLING M=OD

                    Oysters - dredge and hand
                    Sediment - box core

             WATER DEPM - subtidal, 0.3 to 2.5 meters

             POSSIBLE CONTAMINANTS - Obvious sources of contamination included
             extensive oil and gas platforms to the north and west (closer than 0.5 mile) and
             widespread oil and gas development in the overall delta area east of the river.





                                                     319









                ENVIRONMENTAL DATA

                                           Salinity          Temperature         Dates Sampled
                                             (9010)               (OC)

                      'YEAR 1                12.0                 17.0           26 March 1986
                      YEARII                 15.0                 15.0           14 February 1987
                      YEAR 111                8.0                 14.0           25 February 1988
                      YEAR IV                 -                   18.0           8 January 1989
                      YEAR V                 14.0                 12.0           9 January 1990
                      YEAR VI                 4.3                 13.0           11 January 1991
                      YEAR VII               >0.5                 10.0           11 January 1992
                      YEAR VIII               2.0                 10.0           8 January 1993
                      YEAR IX                 1.0                 10.0           13 January 1994
















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                                                 171










               SrIE NUM13ER - 32                              DESIGNATOR - LBM:P

               SITE - MALHEUREUX POINT, LAIKE BORGNE, LA

               NONIINAL SITE CENTER - 29052.02'N              89040.70W


               LOCATED ON NOS CHART             11371

               SITE ACCESS - Access to the site is via LA Highway 39 to Shell Beach. At Shell
               Beach, launch in Bayou Yscloskey and proceed north across the Mississippi River
               Gulf Outlet Canal and into to Lake Borgne. Enter the lake and proceed to the west
               side of Old Fort Beauregard. Run time to the site is less than 0.5 hours.

               SITE DESCRIPTION - Oysters were collected from intertidal waters in mud.
               They were attached to bricks from the historic fort. The site was located on the
               west side of Old Fort Beauregard. Oysters were abundant.

               OYSTER COLLECTIONS

               Year I - Station 1 was in the southeast part of the small cove 100 m west of
                      channel marker 2. Station 2 was 60 m north of Station 1, 150 m west of
                      channel marker 2. Station 3 was 60 m north of Station 2, 200 m west of
                      channel marker 2. Oysters were collected with a commercial dredge.

               Years II, III, X, V, VI, & VII - The oysters were collected by hand along the
                      shore west of the Fort. Station 1 was on the west side by the shore. Station 2
                      was approximately 50 m north of Station 1 by the rock rubble, and Station 3
                      was 70 m west of Station 2 by the mud flat.

               Year VIII & IX - The same stations were collected again these two years, but the
                      oysters were less abundant.

               SEDDMENT COLLECTIONS


               Years I & II - Sediments were taken 300 m offshore in subtidal waters northwest
                      of the channel markers.

               Year III - Sediments were taken 100 m offshore in a north-south transect, with
                      stations 50 m apart. Station I was on the north and Station 3 on the south.

               Year IV - Sediments were not sampled this year.

               Year V - Sediments were composed of soft mud and shell and were collected
                      adjacent (25 m) to each of the oyster stations in 1 m of water.

               Year VI - Sediments were not sampled this year.

               Years VII, VIII, & IX - Sediments were not sampled during these years.



                                                        325










              SAMPLING METHOD

                     Oysters - commercial dredge and hand
                     Sediment - box core and hand

              WATER DEPM - intertidal, 0.1 and 0.3 meters

              POSSIBLE CONTAMINANTS - Contamination factors included outwash from
              the Yscloskey Bayou and Mississippi River Gulf Outlet Canal, an ammunitions
              dump, and oil and gas pipelines.

              ENVIRONAUKNTAL DATA

                                         Salinity          Temperature          Dates Sampled
                                           (%0)                  (OC)

                   YEAR 1                  12.0                  20.0           27 March 1986
                   YEAR 11                 12.0                  20.0           15 February 1987
                   YEAR 111                17.0                  16.0           30 January 1988
                   YEAR IV                  -                    18.0           8 January 1989
                   YEAR V                    4.0                 11.0           8 January 1990
                   YEAR VI                 16.0                  16.0           10 January 1991
                   'YEAR VII               10.0                  14.0           10 January 1992
                   YEAR VIII                 8.0                 16.0           7 January 1993
                   YEAR IX                   6.0                 10.0           14 January 1994



























                                                          326




















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                   NUMBER - 62                              DESIGNATOR - LBNO

              SITE - NEW ORLEANS, LAIKE BORGNE, LA

              NONDNAL SITE CENTER - 29056.60'N              89050.10V


              LOCATED ON NOS CELART # - 11364

              SITE ACCESS - Access is by boat launched at the marina north of Chalmette on
              LA Highway 47. From the marina, travel north to Bayou Bienvenue, then east to
              the first channel or continue on to the Mississippi River Gulf Outlet Canal. Turn
              right (southeast) and continue on for 5 miles, then turn right into Lake Borgne.

              SITE DESCRIPTION - This site was established in Year III and was located on
              the west side of Lake Borgne. The site was located just inside the pass into the
              lake along the south shore and was due south of Martello Castle.

              OYSTERCOLLECTIONS

              Year III - The oyster stations were located along the shoreline from east to west.
                    Oysters were collected by hand in 10-20 cm water at low tide. Live oyster
                    clumps were interspersed with abundant dead shell. Station 1 was due
                    south of Martello Castle. Station 2 was 50 m to the west. Station 3 was
                    another 50 m west.

              Years JrV, V, VI, VII, & VIII - The site was not sampled during these        years.

              Year IX - The site was not scheduled to be sampled this year, but was substituted
                    as an alternate site for LPGO (Gulf Outlet, Lake Pontchartrain). The
                    oysters were large and abundant, and were collected form the same
                    stations as in Year III.


                 3DUNT COLLECTIONS

              Year III - Sediment sites were located 50 m offshore to the north of each oyster
                    station in an east to west line. Sediments were a mixture of peat, mud and
                    shell.

              Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                    years.

              SAMIPLING N=ODS

                    Oysters - hand
                    Sediment- box core

              WATER DEPTH - intertidal, 0.1 - 0.2 meters





                                                     331









           POSSIBLE CONTAMINANTS            No obvious pollution sources were evident.
           However, the Mississippi River Gulf Outlet drained the area from around New
           Orleans as well and carried boat and ship traffic.

           ENVIRONMENTAL DATA

                                 Salinity      Temperature     Dates Sampled
                                  (9100)           (11C)

                YEAR 111           16.0            18.0        31 January 1988
               YEAR EK             7.0              9.0        14 January 1994







































                                               332



















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                                                       Site #62 (LBNO), Lake Borgne New Orleans (from chart 11371).















































                            q(41










              SITE NUMBER - 63                               DESIGNATOR - LPGO

              SITE - GULF OUTLET, LAIKE PONTCHARTRAIN, LA

              NOMNAL SITE CENTER - 30002.44'N                90002.57rW

              1A)CATED ON NOS CELART # - 11369

              SITE ACCESS - A boat is launched at the ramps located under Shoreline Drive
              Bridge. The site is located just to the north of the ramps in the south edge of Lake
              Pontchartrain. Travel time to the site is less than 5 minutes. This site cannot be
              sampled when there is a strong north wind due to high waves.

              SITE DESCRIPTION - An attempt was made in Year III to establish a site at the
              Gulf Outlet on the south shore of Lake Pontchartrain. Although the site was
              recommended as being most likely to support oysters, no oysters were present;
              therefore, no stations were established nor was sediment collected. Based on new
              information, a sampling site was established in Year VI near the Gulf Outlet on
              the south side of Lake Pontchartrain. The site was located approximately 1 km
              north of the bridge adjacent to the New Orleans Lakefront Airport. Oysters
              occured at this site due to a salt water wedge that entered through the outlet. The
              high salinity water allowed the oysters to exist on the bottom near the Gulf outlet.

              OYSTERCOLLECTIONS

              Year VI - The oyster stations were located along the edge of the channel in deep
                     water. Oysters were sparse this year because the Louisiana Fish and
                     Wildlife Department had removed most of them in a replanting project.
                     Stations were not distinguished due to the sparse population. Three hours
                     of dredging was required to obtain 90 oysters. This station should improve
                     in future years since the area is closed to commercial luggers.

              Years VII & VIII - Oysters were not collected at during these years.

              Year IX - Only three live oysters were found after three hours of dredging. As a
                     result, an alternate site was selected - LBNO (New Orleans, Lake Borgne)
                     for sampling.

                 DEVIENT COLLECTIONS

              Year VI - The only suitable sediments were found in deep water along the edge of
                     the channel. All of the south end of the Lake had hard packed sandy
                     bottom. Station 2 was located at the coordinates given for the oyster
                     collection. Station 1 was located 200 m to the northwest and Station 2 was
                     300 m to the east. Sediments can only be obtained by a box corer equipped
                     with a 10 m rope and a messenger.

              Years VII, VIII, & IX - Sediments were not sampled during these years.



                                                      337










           SAMPLINGMETHODS

                 Oysters - dredge
                 Sediment - box core attached to a rope

           WATER DEPTH - Oysters 4 to 5 m and greater than 5 m for sediment.

           POSSIBLE CONTAMINANTS - No obvious pollution sources were evident, but
           Lake Pontchartrain drained through this outlet and tidal water from New
           Orleans flushed back into the lake. There was also abundant boat and ship
           traffic.


           ENVIRONMENTAL DATA

                                 Salinity      Temperature     Dates Sampled
                                  (9010)           (0c)

                YEAR VI            5.0             11.0       31 January 1988
                YEAR IEK           5.0              9.0        13 January 1994

































                                              338

























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                                             M[ISSISSIPPI SrIES

              SrIE NUMBER 33                                 DESIGNATOR - MSPC

              SITE - PASS CHRISTIANY MISSISSIPPI SOUND, MS

              NONEINAL SYIE CENTER - 30018.12'N              89019.62W

              LOCATED ON NOS CHART # - 11372@ 11373

              SITE ACCESS - Access point in Year I was from Pass Christian small craft
              harbor with an approximate running time of 20 minutes. From Year II on,
              access to the site was via automobile, taking the first exit on the west side of the
              bridge at Highway 90 and following the road south along the shoreline to Veterans
              Pier. A boat ramp was located on the south side of the jetty/pier for sediment
              sampling.

              SITE DESCRIPTION - In the first year oysters were tonged from the open water
              offshore from Bay St. Louis (30"17.75'N and 89'19;60'W). The original reef location
              off Pass Christian was dead in Year II and the site was moved onshore to the
              Veterans Pier. At the pier, oysters were attached to rocks and on the muddy sand
              bottom. Accessibility was best at low tide.

              OYSTER COLLECTIONS

              Year   I - Year I stations were located in Bay St. Louis. Station 1 was
                     approximately 0.5 to 0.75 miles south of the railroad bridge. Bearing to
                     landmarks were: 70' to the silver water tower, 230' to the center pivot
                     trestle, and 350' to the buoy marker. Station 2 was 200 m north of Station 1
                     and Station 3 was 200 m farther north.

              Years II & III - During Year II no oysters were found at the Year I site. In
                     Years II and III, the site was moved to the Veteran's Pier at Bay St. Louis.
                     The oysters were intertidal and were collected by hand without the use of a
                     boat. Station I was on the north side of the jetty. Station 2 on the seaward
                     side of the "T" end of the jetty and under the pier. Station 3 was on the
                     south side of the jetty.

              Year IV - In Year IV, the site was the same as in Year II & III, on Veteran's
                     pier. Stations were located as before. Construction was in process along
                     the shore, jetty and pier and sand had been filled on the beach. Additional
                     rock rubble had been added to the "T" end of the jetty, covering most of the
                     oysters at Station 2. However oysters were more plentiful and larger than
                     in the past.

              Year V - Stations were in the same locations as previously sampled. Oysters were
                     very scarce due to the recent freeze which had killed all oysters above the
                     low tide line.





                                                       343









              Year VI - There were no live oysters at Stations 2 and 3. At Station 1 there were
                    very few live oysters attached to the concrete rubble but there were
                    numerous oysters at a small reef 20 m north of the north end of the "T" end
                    of the jetty. All three stations were collected on this small reef. Stations
                    were not differentiated.

              Year VII - This site was not sampled this year. No live oysters could be found at
                    any of the previously sampled sites nor in the surrounding bay.

              Year VIII - No oysters were present at the Veteran Pier, so the site was moved
                    1.65 nm to the NE (bearing 57). The site is located at a submerged reef
                    which parallels the Highway 90 bridge. The reef lies 10 meters south of the
                    bridge on the east side of the channel in 2.5 m of water. Oysters were
                    numerous but since the reed is small, individual stations were not
                    recognized. Loran coordinates for the new reef are 30' 18.87'N and 89'
                    18.05V.

              Year IX - Veteran's Pier was re-occupied this year, as there was an abundance of
                    small oysters again.The sampled stations were the same as those used in
                    Years II through V.

                 3D4E'NT COLLECTIONS

              Year I - Sediments were taken at 100, 150 and 175 m south, respectively, from
                    each oyster station.

              Year 1I - Sediments were obtained by box coring directly offshore from the jetty at
                    distances of 200, 400, and 600 m, respectively, for the three stations.

              Year III - Sediment stations were collocated with each oyster station. Silty-sand
                    sediments were collected along the shoreline and can be reached by
                    wading.

              Years IV, V, VI, VII, VIII, & LX - Sediments were not sampled during these
                    years.

              SAMPLING METHOD

                    Oysters - tonging and hand
                    Sediment - box core and hand

              WATER DEPTH - 0 to 3 meters (subtidal in Year 1, intertidal in succeeding years)

              POSSIBLE CONTAMINANTS - There were no obvious sources of contamination
              nearby.






                                                     344










               ENMONMENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (%0)                  (OC)

                     YEAR 1                 11.3                  16.0           6 March 1986
                     YEARII                   8.0                 15.0          16 February 1987
                     YEAR 111               18.0                  17.0          2 February 1988
                     YEAR IV                16.0                   -            10 January 1989
                     YEAR V                   9.0                 16.0          10 January 1990
                     YEAR VI                17.3                  11.0          12 January 1991
                     YEAR VIII              10.0                  15.0          8 January 1993
                     YEAR ICK               18.0                  6.0           15 January 1994



















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                                                                      Site #33 (MSPC), Mississippi Sound Pass Christian.










                                           I U.S. Coast Guard Light List for supplemental
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              SITE NUMBER 34                                 DESIGNATOR - MSBB

              SITE - BILOXI BAY, MISSISSIPPI SOUND, MS

              NONDNAL SYIE CENTER - 30023.55'N               88051.45V

              LOCATED ON NOS CHART # - 11373,11372

              SITE ACCESS - No boat is necessary since this is a walk up site. Access to the site
              is via automobile, parking at the Marine Education Center on Highway 90 at the
              Biloxi-Ocean Springs bridge.

              SITE DESCRIPTION - The sample area was along the shoreline in intertidal
              waters. Oysters were taken from the concrete bulkhead, rocks and debris in the
              water. There were a limited number of oysters at this location.

              OYSTER COLLECTIONS

              Years I, II, & III - The stations established in Year I were collected the next two
                     years also. Station 1 was adjacent to Marine Education Center. Station 2
                     was next to the west end of the bridge on the south side. Station 3 was
                     northeast of the bridge - 150 m from Station 2.

              Year IV - In Year IV, the stations were: Station 1, under the bridge at the second
                     and third support from the west end; Station 2, at the first pier support
                     under the bridge; and Station 3, on the shoreline bulkhead and into the bay.
                     As before the oysters were collected by hand from the concrete pilings, piers
                     and concrete rubble.

              Year V - Stations 2 and 3 were in the same locations as sampled last year. Station
                     1 was moved from under the bridge to 125 m up the shoreline to near the
                     point north of the bridge. This station was moved due to the lack of live
                     oysters. A freeze had recently killed most of the oysters.

              Year VI - Stations 2 and 3 were again at the locations sampled last year. Station I
                     had not recovered from the freeze, so it was collected north of the bridge on
                     a concrete bulkhead. Oysters were numerous at this site.

              Year VII - No live oysters could be found at any of the stations this year.

              Year VIII - Oysters were again present and were collected at the locations
                     described for Year V.

              Year IX - The site was not scheduled to be sampled this year.










                   31M[ENT COLI.EMONS

               Years 1, H, & III - Sediments were taken offshore from the three oyster sampling
                      locations, with the boat being launched at the city marina -0.25 mile to the
                      east from the Mareing Education Center.

               Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                      years.

               SAMPLING NIETHOD

                      Oysters - hand
                      Sediment - box core

               WATER DEPM - intertidal, 0. 1 to 0.3 meters

               POSSEBLE CONTAMINANTS - Possible contamination factors included marine
               traffic and city pollution.

               ENVIRONAEEMAL DATA

                                          Salinity          Temperature         Dates Sampled
                                                                 (OC)

                    YEAR 1                  15.0                 15.0            4 March 1986
                    YEAR 11                 15.0                 15.0           16 February 1987
                    YEAR 111                16.0                 14.0           2 February 1988
                    YEAR IV                 14.0                 17.0           10 January 1989
                    YEAR V                    5.5                15.0           11 January 1990
                    YEAR VI                   3.0                14.0           12 January 1991
                    YEAR VIII               10.0                 16.0           8 January 1993




















                                                           351

















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              SrIE NUMBER 35                                DESIGNATOR - MSPB

              SITE - PASCAGOULA BAY, MISSISSIPPI SOUND, MS

              LATI'I'UDE 30020.03'N                 88036.10V

              LOCATED ON NOS CHART# - 11374,11373

              SITE ACCESS - To reach the boat ramp on the Pascagoula River turn off Highway
              90 onto Market Street going south. When the road dead-ends at the beach, turn
              right and drive west to the end of the road. The boat is launched at the public
              ramp. Then by boat proceed west to red channel marker 4. Run time to the site is
              less than 10 minutes.

              SITE DESCRIEPTION - The site was located west of the new causeway going to the
              Naval Base near the mouth of the West Pascagoula River. Oysters were generally
              abundant and were found by poling the subtidal reef. Soft mud sediments were
              found adjacent to the reef at each collection point. Oysters were abundant.

              OYSTERCOLLECTIONS

              Years I, II, & III - Year I stations were centered around red channel marker 4.
                     Station 1 was 100 m east of red channel marker 4. Bearing to permanent
                     landmarks were: 80' to grain elevators and 260' to Bellefontaine Point
                     Lighthouse. Station 2 was at the red channel marker 4 (80' to grain
                     elevators; 2550 to the lighthouse ). Station 3 was 150 m west of red channel
                     marker 4 (80' to the grain elevators and 262' to the lighthouse). Oysters
                     were collected by tonging.

              Year IV - As in Year III, the site was centered around channel marker 4 which
                     was just west of the new causeway and west of Ingalls shipyard. Station 1
                     was about 100 m east of the marker in shallow water which had been
                     affected by silting from dredging operations. Oysters were smaller and
                     fewer live ones were present than at other stations. Station 2 had to be
                     moved from the marker to about 200-300 m north of the marker where a
                     small iron pipe and a cane pole marked the channel. Oysters here were
                     prolific and fairly large. Station 3 was 150 m west of the marker. It also
                     had oysters in abundance. The whole area around the site was closed to
                     oystering by the health department. Oysters were collected by dredging.

              Year V - Stations were located in the same areas as sampled last year. Oysters
                     were collected by tongs this year.

              Year VI - The site was centered around channel marker 4 (30'20.55,N,
                     88'35.96'W). Station 1 was 100 to 200 m east of the marker. Station 2 was at
                     channel marker 4. Station 3 was 100 to 200 m west of the channel marker.
                     Oysters were very abundant at all three stations.





                                                      356










               .Years VII & VIII - All three stations were centered around channel marker 4.
                      Station 1 was in the same area as last year, 100 to 150 m east of the marker.
                      Station 2 was moved slightly north (50 to 150 m) of the marker. Station 3
                      was 150 m west of the marker. Oysters were abundant at all three stations.

               Year IX - No oysters were found in the vicinity of channel marker #4. A new
                      location was found off the NW comer of the Naval Base Island, next to the
                      new causeway (30'20.14'N, 88135.17'W). Station 1 was 100 m offshore next to
                      the causeway, Station 2 was 50 m further south and Station 3 another 50 m
                      to the south. The oysters were abundant and easily dredged.

                   )IMENT COLLECTIONS

               Years 1, 11, & III - Sediments were gray and brown mud mixed with sand.
                      Collection sites were north of each site within 75 to 100 m.

               Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                      years.

               SAMPLING METHOD

                      Oysters - tongs and dredge
                      Sediment - box core

               WATER DEPTH - subtidal, 1 to 1.5 meters

               POSSIBLE CONTAMINANTS - Possible contamination sources included the
               Pascagoula River, City of Pascagoula, Naval Ship Yard, heavy marine traffic and
               nearby industries.

               ENVIRONMENTAL DATA

                                         Salinity          Temperature          Dates Sampled
                                            (%0)                 (OC)

                    YEAR 1                  19.0                 14.0            5 March 1986
                    YEAR 11                 19.0                 14.0           16 February 1987
                    YEAR 111                5.0                  12.0           15 February 1988
                    YEAR IV                 2.0                  14.0           10 January 1989
                    YEAR V                  1.0                  12.0           12 January 1990
                    YEAR VI                 6.0                  11.0           13 January 1991
                    YEAR VII                22.0                 14.2           12 January 1992
                    YEAR VIII               6.0                  12.0           9 January 1993
                    YEAR IX                 18.0                 11.0           15 January 1994








                                                           357



















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                                              ALABAMA SITES

              S= NUMBER - 36                                 DESIGNATOR - MBCP

              SHE - CEDAR POINT REEF, MOBILE BAY, AL

              NOMINAL SITE CENTER - 30018.70'N               88008.OOW

              LOCATED ON NOS CHART # - 11376,11378

              SITE ACCESS - Boat launch is at the small bridge just north of the Dauphin
              Island causeway bridge. The site is located directly east of the middle part of
              Cedar Point. At low tide the site can be reached by walking across the shallow
              sandbar on the north end of the reef. By boat the site is 10 to 15 minutes from the
              launch site.

              SITE DESCRIPTION - The site was located on an oyster reef 0.25 mile offshore
              and east from the highway leading to the causeway bridge to Dauphin Island.
              The reef was almost due east of the Cedar Point fishing pier which was on the
              west side of the highway.

              OYSTERCOLLECTIONS

              Year I - Oysters were tonged from 2-3 m of water at a site approximately 0.75
                     miles east of the Dauphin Island highway. Station I was at bearings 180' to
                     center of ICW bridge; 280' to Alabama Port Point, and 134* to Fort Gaines.
                     Station 2 was 500 m to the south at bearings 1800 to the center of ICWW
                     bridge; 283' to Alabama Port Point and 133' to Fort Gaines. Station 3 was
                     300 m southeast of Station 2. Bearing to local landmarks were 178' to center
                     of the ICWW bridge; 278' to Alabama Port Point, and 122' to Fort Gaines.

              Year II - Sampling stations were distinguishable during Year I but not in Year
                     II because of the scarcity of the oyster population. Oysters were purchased
                     from a commercial tonger who had been working for 7 hours to tong only
                     180 oysters.

              Year III - Year III oysters were tonged east of the causeway on a submerged reef.
                     Station 1 was on the south end of the reef. Station 2 was 50 m north of
                     Station 1 and Station 3 was another 75 m to the north. There were several
                     oystermen tonging in the immediate vicinity.

              Year IV - In Year IVI the oysters were sampled by hand at low tide on a sand and
                     shell reef to the east of the causeway adjacent to the Cedar Point fishing
                     pier. Station 1 was at the south end of the reef; Station 3 was at the north
                     end; Station 2 was in the middle. Total distance from Stations 1 to 3 was 150
                     m and water depth at low tide was 0.2 m. The oysters were not abundant.









             Year V - Sampling stations were located on the same reef in the same positions as
                    sampled last year. Oysters were very sparse, perhaps because of the recent
                    freeze.
             Year V1 - Oysters were collected at the same locations again this year. Oysters
                    were more abundant this year.

             Years VII & VIII - Oysters sites were at the same locations as described in Year
                    IV.

             Year IX - This was not scheduled to be visited this year, however it was sampled
                    as an alternative to BBTB (Turtle Bay, Barataria Bay). The oysters were
                    collected from the same locations as in Years IV through VIII.

                 )IMENT COLLEMONS

             Year I - Sediment sites in Year I were 25, 125 and 115 m east, respectively, for
                    each station.

             Year II - Sediment sites were south from the oyster sites in close proximity to the
                    bridge pilings. Station 1 was located at the eighth bridge pillar from the
                    north end of causeway; Station 2 was 0.25 mile south of the north end of the
                    causeway bridge and 25 m to the east; and Station 3 was taken east of the
                    south end of the cut-off bridge where the boat was launched.

             Year III - Sediments were collected adjacent to the bridge. Station 1 was at the
                    eighth bridge pillar from the north end of the causeway. Station 2 was at
                    the second set of telephone poles east of the causeway. Station 3 was on a
                    submerged reef 75 m north of Station 2. The bottom was primarily sand,
                    shell and silt.

             Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                    years.

             SAMPLING METHODS

                    Oysters - tongs and hand
                    Sediment - box core

             WATER DEPTH - intertidal to subtidal, 0.2 to 1.2 meters.

             POSSIBLE CONTAMINANTS - No obvious sources of contaminants were
             observed.












                                                     363










                ENVIRONhUENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                                                  (OC)

                     YEAR 1                 15.0                  16.0           4 March 1986
                     YEAR     11             6.0                  15.0          16 February 1987
                     YEAR     111            5.0                  14.0          2 February 1988
                     YEAR     IV             5.0                  14.0          10 January 1989
                     YEAR V                 11.0                  14.0          9 January 1990
                     YEAR VI                 8.0                  13.0          12 January 1991
                     YEAR VII               14.0                  13.0          12 January 1992
                     YEAR     VIII           7.0                  14.0          9 January 1993
                     YEAR IEK               20.0                  11.0          15 January 1994






































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              SrIE NUMBER 66                                DESIGNATOR - MBFII

              SITE - HOLLINGERS, ISLAND CHANNEL, MOBILE BAY, AL

              NONEINAL SHE CENTER - 30033.80'N              88004.50V

              LOCATED ON NOS CE[ART           11376

              SITE ACCESS - The launch point is at Dog River which is south of 1-10 on the
              Dauphin Island Parkway. Launch at the Beach Comber and proceed out the Dog
              River channel to channel marker 10. Turn south and start poling for oysters.

              SITE DESCRIPTION - This collection site established in Year III was in the
              upper reaches of Mobile Bay and was near the upper distributional limit of oysters
              in the bay.

              OYSTER COLLECTIONS

              Year  III - Oysters were located in 2 m of water on small scattered reefs with
                    oysters, shell and clams. Oysters were difficult to locate and were collected
                    by tonging. Station 1 was directly south of red buoy 10 in the Dog River to
                    Biloxi channel. Station 2 was 75 m to the south of Station 1. Station 3 was
                    another 75 m south.

              Year  IV - In Year IV, the oysters were collected by tonging on a small and
                    sparsely populated reef about 100 m southwest of channel marker 9 and an
                    equal distance southeast from a 4 legged wooden stand, just east of the Dog
                    River marina. Station 1 was at the north end. Station 3 was at the south
                    end, and Station 2 was in the middle. Water depth was 2 m, and the reef
                    can only be located by poling the bottom. Oysters came up mostly from the
                    shelly mud bottom as singles, and large aggregations were not found. The
                    bottom was a mixture of sand, mud and shell. This site is best collected in
                    calm weather.

              Year V - Oyster stations were in the same general locations as sampled last year.
                    The oysters were sparse. This site required all day to complete since it
                    required poling and tonging to locate and collect the oysters.

              Year VI - Oysters were collected at the same stations described in Year IV
                    (30033.88'N, 88004.58'W).

              Years VII, VIII, & IX - Oysters were collected at the same stations described in
                    Year IV, but were dredged these years. Oysters are still not very abundant
                    during any of these years.

                 DIMENT COLLECTIONS

              Year 111 - Sediment stations are collocated with each oyster station and must be
                    located by poling.



                                                      369










               Years IV & VI - Sediments were not sampled during these years.

               Year V - Sediment samples were taken at the same locations as the oyster
                      samples.

               Years VII, VIII, & LK - Sediment samples were not taken during these years.

               SAMPLING Affi'THODS

                      Oysters - tongs
                      Sediment - box core

               WATER DEPTH - subtidal, 2 to 2.5 meters

               POSSIBLE CONTAMINANTS - Contamination in the region was from ship
               traffic, industry, and inflow from the Dog River which drained much of Mobile.
               The upper part of the bay was heavily influenced by the Mobile River and the site
               was in an area closed to oystering by the Department of Health.

               ENV][RONhW,NTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                  (OC)

                    YEAR 111                6.0                  13.0          15 February 1988
                    YEAR IV                 6.0                  15.0          10 January 1989
                    YEAR V                  2.0                  12.0          10 January 1990
                    YEAR VI                 5.0                  15.0          12 January 1991
                    YEAR VII                7.0                  12.0          12 January 1992
                    YEAR VIII               5.0                  14.0          9 January 1993
                    YEAR IEK                7.0                  9.0           15 January 1994




















                                                           370

















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               SHE NUMBER 79                                 DESIGNATOR - MBDR

               SITE - DOG RIVER, MOBILE BAY, AL

               NOAHNAL SITE CENTER - 30035.50'N              88002.72'W

               LOCATED ON NOS CHART            11376

               SITE ACCESS - The launch point is at Dog River which is south of I-10 on the
               Dauphin Island Parkway. Launch at the Beach Comber and proceed out the Dog
               River channel to The Mobil Ship Channel and go north to green channel marker
               69. Turn into the short channel west of marker 69 and proceed to channel marker
               5. The site is located approximately 75 m on a bearing of 280* from channel
               marker 5

               SITE DESCRIPTION - This collection site, established in Year V, was in the
               upper reaches of Mobile Bay and was near the upper distributional limit of oysters
               in the bay. The site was located on a reef which runs west-southwest from
               channel marker 5 for approximately 300 m. Poling was required to locate the
               oysters.

               OYS= COLLECTIONS

               Year V - Oysters were located in 1.5 m of water on small scattered reefs with
                     oysters, shell and clams. Oysters are difficult to locate and are collected by
                     tonging. Station 1 was on the southwest end of the reef. Station 2 was
                     approximately 100 m to the northeast. Station 3 was another 100 m
                     northeast of station 2.

               Years VI, VII, VIII, & IX - Oysters were not sampled during these years.

               'F F D&ENT COLLECTIONS

               Year V - Sediment samples were taken 50 m north of each of the oysters stations.
                     Sediments were a mixture of mud, shell and sand.

               Years VI, VII, VIII, & IX     Sediment samples were not collected during these
                     years.

               SAWLING METHODS

                     Oysters - tongs
                     Sediment - box core

               WATER DEPTH - subtidal, 1.5 meters

               POSSIBLE CONTAMINANTS - Contamination in the region was from ship
               traffic, industry, and inflow from the Dog River which drained much of Mobile.



                                                       375









            The upper part of the bay was heavily influenced by the Mobile River and the site
            was in an area closed to oystering by the Department of Health.

            ENVIRONMENTAL DATA

                                  Salinity       Temperature      Dates Sampled
                                    OYOV)             (OC)

                YEAR V              2.0               12.0        10 January 1990









































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                                           Site #79 (MBDR), Mobile Bay Dog River (from chart 11376)
























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                                                FLORIDASITES

               SrIE NUMBER 67                                DESIGNATOR - PBPH

               SITE - PUBLIC HARBOR, PENSACOLA BAY, FL

               NONONAL SITE CENTER - 30024.63'N              87011.42'W

               LOCATED ON NOS CELART           11382

               SITE ACCESS - Access to the site is by boat launched at the public ramp northeast
               of the Highway 98 bridge and the Pensacola Bay Pier.

               SITE DESCRIPTION - This collection site was established in Year III. It was
               located in Pensacola under the Pensacola Fishing Pier which was the old
               Highway 98 bridge. The pier was on the east side of the new bridge. Collections
               from this site were easiest at low tide and in calm weather since the boat had to be
               tied to the piers.

               OYSTER COLLECTIONS

               Years III & IV - Oysters were collected by hand from concrete piling under the
                     bridge. Oyster Station 1 was at the seventh set of pilings from the north
                     shore. Station 2 was at the eleventh set of pilings. Station 3 was at the
                     fifteenth set of pilings. All oysters collected were intertidal.

               Year V - Oysters were collected in the same locations as sampled last year. There
                     were many recently dead oysters on the upper part of the piling. This was
                     primarily due to the recent freeze.

               Years VI & VII - Oysters were collected at the same locations as previously
                     sampled. Oysters were larger and more abundant.

               Years VIII & IX - Oysters were collected at the same stations previously sampled,
                     but were more abundant.


                  3DIENTCOLLECTIONS

               Years III, IV, & V - Water depth at the site was -7 m. No sediments were
                     collected because of the depth. Substrate near the boat ramp was sandy.

               Year VI - No sediment samples were collected this year.

               Year VII - Sediment samples were collected for the first time at 30'24.14'N and
                     87111.38W. Suitable sediments were located in 6.2 m of water and were
                     collected with a box corer. Three stations were collected 50 m apart on a
                     line starting at the 45 mph sign on the bridge running toward the north.

               Years VIII & IX - No sediment samples were collected during these years.


                                                       381








              SAMPLING MIETHOD - Oysters were collected by hand from the concrete pilings
              of the old bridge in the intertidal zone. The site can only be collected in calm
              weather since the oysters are collected by hand from the boat.

              WATER DEPTH - intertidal, 0 to 0.4 meters

              POSSEBLE CONTAMINANTS - Contamination was likely from several sources
              including both municipal and marine traffic.

              ENVIRONAIENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (9100)               (OC)

                    YEAR 111               22.0                 12.0           19 February 1988
                    YEAR IV                19.6                 15.0           25 January 1989
                    YEAR V                   9.0                14.0           26 January 1990
                    YEAR VI                21.0                 12.0           26 January 1991
                    YEAR VII                 8.0                9.0            22 January 1992
                    YEAR VIII              18.0                 16.0           11 January 1993
                    YEARIX                 24.0                 10.0           16 January 1994








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                                                  Site #67 (PBPH), Pensac6la Bay Public Ha           .rbor.

 DDeLorme Ma"ing Company. Map Taken from Florida Atlas and Gazeneer. Reproduced with Permission-














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               SITE NUMIBER - 37                               DESIGNATOR - PBIB

               SITE - INDIAN BAYOU, PENSACOLA BAY, FL

               NONEINAL SrIE CENTER - 30031.00'N               87006.70W

               LOCATED ON NOS CHART # - 11382,11378

               SrIE ACCESS - Access to the site is by automobile off 1-10 on Avalon Blvd. Drive to
               the Indian Bay Marina and tum right on Connido Street. Proceed to a dirt road
               intersection, turn left and proceed to a dead end at Indian Bayou. Launch the boat
               at the small ramp and proceed down Indian Bayou to its mouth. In Year IV, the
               mouth of Indian Bayou had silted in, so the launch point was moved. To reach
               the boat ramp, exit I-10 at Avalon then go north to SR 281. Tum left and go to the
               stop sign, tum left again and follow the dirt road until it ends at the Archie Glover
               Park. To reach the site, follow the marked channel out to Escambia Bay and then
               turn south going under the bridge to the reef The reef is located about 1 mile
               south of the bridge on a bearing of 140'. Run time to the site is approximately 20
               min.

               SITE DESCRIPTION - There was generally an abundant supply of oysters at this
               location. Year I oysters were collected by hand near'the mouth of Indian Bayou at
               3031.35'N and 87'06.38'W. In Year II the site was moved to a more permanent
               location. Oysters sites were located on a subtidal reef west of the entrance to
               Indian Bayou. The site was marked by several pilings, one of which had an
               "Oyster Reef' sign. The site was located between the poles marked "Danger,
               Oyster Reef."

               OYS41ER COLLECTIONS

               Year I - Year I oysters were collected by hand near the mouth of Indian Bayou.
                     Station 1 was 30 m north of Indian Bayou. Bearing to landmarks useful in
                     locating the site were: 292' to factory towers; 249' to the water tower. Station
                     2 was 50 m south of Indian Bayou (290' to the factory towers; 248* to the
                     water towers). Station 3 was 30 m south of the bayou mouth in the bayou.

               Years II, III, & IV - The site was moved out into the bay in Year II. Oysters were
                     tonged from three locations along the reef. Station 1 was at the northeast
                     end of the reef-, Station 2 was 100 m southwest of Station 1, near the poles
                     marking the reef; Station 3 was 100 m southwest of Station 2, near the
                     "Danger" sign.

               Year V - No oysters were available for collection at this site due to high volumes of
                     freshwater inflow which had recently killed all of the oysters.

               Year VI - The oyster reef was recovering from the dieoff experienced in Year V.
                     Station 1 was at the northeast end of the reef between the two poles marking
                     the edge of the reef. Station 2 was on the middle of the reef between the two
                     middle poles. Station 3 was on the south end of the reef due north of the last



                                                        387








                      pole. This narrow reef was oriented north to south. Oysters were mixed in
                      black silty mud that has apparently silted over the underlaying shell reef.

               Years VII, VIII, & IX - The oyster reef was still recovering from the dieoff
                      experienced in Year V. Oysters were only found at Station 2 which was
                      located in the middle section of the reef between the two middle poles.

                   )DIENTCOLLECTIONS

               Year I - Sediments in the area were primarily shell and sand. The best source of
                      fine grained sediment was located in Indian Bayou. In all years,
                      sediments were taken from Indian Bayou. In Year 1, sediment stations
                      were collocated with the oyster stations.

               Years II & III - Sediments were taken as follows: Station 1 was from the narrow
                      bayou opening to the bay. Station 2 was along the north shore of the bayou
                      300 m from the mouth and 30 m from shore, and Station 3 was 100 m
                      northeast of Station 2.

               Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                      years.

               SAMPLING METHOD

                      Oysters - hand, tongs and dredge
                      Sediment - hand or box core

               WATER DEPTH - intertidal, 0.3              1 meters (Year I), subtidal, 2 meters, in
                      subsequent years.

               POSSIBLE CONTAMINANTS - There were no observable sources of
                      contamination.


               ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                 (0c)

                    YEAR 1                 10.3                 16.0            3 March 1986
                    YEAR 11                13.0                 13.0           28 February 1987
                    YEAR 111               14.0                 14.0           16 February 1988
                    YEAR IV                27.0                 17.0           25 January 1989
                    YEAR V                   4.0                15.0           27 January 1990
                    YEAR VI                25.0                 12.0           26 January 1991
                    'YEAR VII                4.0                10.0           22 January 1992
                    YEAR VIII              23.3                 15.0           11 January 1993
                    YEAR IEK               23.0                 11.0           16 January 1994




                                                          388

















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    DDeLorme Mapping Company. Map Taken from Florida Ada& and Gazetteer. Reproduced with pernlimion.













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               SUE NUMER 80                                   DESIGNATOR - PBSP

               SITE - SABINE POINT, PENSACOLA BAY, FL

               NONUNAL SITE CENTER - 30020.80'N               87009.10V

               LOCATED ON NOS CHART # - 11382

               SITE ACCESS - Access to the site is by boat which is launched at the public ramp
               in Gulf Breeze. To reach the site cross the Pensacola Bay Bridge on Highway 98,
               go through Gulf Breeze to Shoreline Drive. Turn right and go to the Shoreline
               Drive Park. Turn left and continue to the boat ramp on the north side of the Santa
               Rosa Sound.


               SITE DESCRIPTION - This collection site established in Year V is located in
               Santa Rosa Sound. The site is located under the Highway 399 bridge on the south
               side of the ICWW. Collections from this site are easiest at low tide and in calm
               weather since the oysters are attached to the concrete piers.

               OYSTER COUZCTIONS

               Year V - Oysters were collected by hand from concrete piling under the bridge.
                     Oyster Station 1 was the first 7 sets of piers on the south side of the bridge.
                     Station 2 was directly north of the first station and included the next nine
                     large concrete piers. Station 3 was the next eight piers to the north on
                     Station 2. The site ends at the channel. All oysters collected were
                     intertidal. Numerous oysters were dead due to the recent freeze.

               Years VI & VII - Oysters were not sampled these years.

               Year VIII - Oysters were collected at the same stations as described for Year V.

               Year LX - The site was not scheduled for sampling this year.

               SEDDMENTCOLLECTIONS

               Year V - Sediments were collected approximately 1 mile to the northeast, along
                     the shoreline at latitude 30'21.03'N and longitude 87'09.35W. Sediments
                     were primarily sand.

               Years VI, VII, VIII, & IX - No sediments were samples were collected during
                     these years.

               SAMPLING MIETHOD - Oysters were collected by hand from the concrete pilings
               of the old bridge in the intertidal zone. The site can only be collected in calm
               weather since the oysters are collected by hand from the boat.

               WATER DEPTH - intertidal, 0 - 0.4 meters




                                                        393








           POSSIBLE CONTAMINANTS - Contamination was possible from municipal and
           marine traffic.


           ENVERONAIENTAL DATA

                                Salinity      Temperature     Dates Sampled
                                  (9010)           (OC)

                YEAR V            15.0             15.0       26 January 1990
                YEAR VIII         25.0             15.7       11 January 1993







































                                              394












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                                                             Site #80 (PBSP), Pensacola Bay Sabine Point.


  )DeLmme Mapping Company. Map Taken from Florida Aflas and Gazetteer. Reproduced with permission-
















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              SrIE NUMBER - 73                              DESIGNATOR - CBJB

              -SITE - JOE'S BAYOU, CHOCTAWARATCHEE BAY, FL

              NONGNAL SITE CENTER - 30024.62'N              89029.45V

              LOCATED ON NOS CHART # - 11388,11385

              SITE ACCESS - The location is a walk-up site and is reached by driving up Beach
              Drive to the boat ramp. The site is located just south of the boat ramp.

              SITE DESCRIPTION - The site which was established in Year IV was on the
              south shore of Choctawahatchee Bay between the other two Choctawhatchee Bay
              sites. The site was located at the mouth of Joe's Bayou where it enters the Bay.
              The collections were taken from the area directly south of the boat ramp, the
              oysters were intertidal and accessible by wading from shore. More oysters were
              available farther up the bayou along the shoreline structures. Access to these
              sites had to be by boat because the surrounding grounds were all private homes.

              OYSTERCOLLECTIONS

              Years IV & V - The three stations were along the shoreline with Station 1 at the
                    launch/pier; Station 2 next; and Station 3 as far south as one can go before
                    encountering the private dock. The total distance spanned only 50-100
                    meters.

              Year VI - The same stations as were sampled in Year V were collected this year.
                    Oysters were numerous but small.

              Years VII, VIII, & IX - The same stations as described in Year IV were sampled
                    during these years.

              SEDD4ENT COLLECTIONS

              Year IV - No sediments of anything other than hard packed sand or fresh humic
                    materials composed primarily of pine needles could be found. The upper
                    reaches of the bayou and the mouth out into the bay were checked. No
                    sediments were obtained because none met the sampling criteria.

              Year V - Suitable sediments were located in the southern arm of the upper part of
                    Joe's Bayou in 1.5 m of water. Station 1 was 30 m from the west shoreline
                    between the wood boathouse and the two boat pier to the north. Bearing to
                    locate the station was 120' to the Destin water tower. Station 2 was in the
                    northeast arm of upper Joe's Bayou near the end on the north shore
                    between the last and next to last boathouse. Water depth was 1.2 m and the
                    sediment was very loose brown silt. Station 3 was across the bayou (east)
                    from Station 2, between last boat house and boat house #016.




                                                      399








               Years VI, VII, VIII, & LK - No sediments were sampled during these years.

               SAMPUNGM=ODS

                      Oysters - hand
                      Sediment - box core

               'WATER DEPTH - intertidal, 0 to 0.6 meter

               POSSIBLE CONTAMINANTS - There appeared to be no drainage other than
               rainfall runoff from the immediate area into the bayou, and its proximity to the
               bay suggested that it was fairly representative of the bay. No obvious sources of
               contaminants were apparent except for a sand and gravel transfer yard
               immediately to the north of the site.

               ENVIRONM]KNTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (IYOO)               (0c)

                    YEAR IV                  0.3                16.0           27 January 1989
                    YEAR V                   6.0                14.0           27 January 1990
                    YEAR VI                15.0                 12.0           27 January 1991
                    YEAR VII               24.0                 12.0           22 January 1992
                    YEAR VIII              12.0                 17.0           12 January 1993
                    YEAR IEK               24.0                 11.0           17 January 1994







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                                                                                                             Site #73 (CWB), Choctawhatchee Bay Joes Bayou.


        Wel,40rfne Mapping Company. Map Taken from Florida Atlas and -Gazettezr. Reproduced with permission.









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                                          Site #73 (CBJB), Choctawhatchee Bay Joes Bayou (f
























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               SM NUMER M                                     DESIGNATOR - CBSR

               SITE - OFF SANTA ROSA, CHOCTAWHATCHEE BAY, FL

               NOMINAL SITE CENTER - 30024.78rN               86012.25rW

               LOCATED ON NOS CELART # - 11388,11385

               SITE ACCESS - The site is located in the eastern portion of Choctawhatchee Bay
               near U.S. Highway 331. Access to the site is by boat launched from ramps on
               either side of the south end of the bridge crossing Choctawatchee Bay or at the
               Yodum's Grocery ramp north of the drawbridge. From the drawbridge proceed
               west to channel marker 34 on the ICWW.

               SITE DESCRIPTION - Two distinct locations have been sampled for this site. In
               Year I, oysters were tonged south of the channel marker 34. The subtidal oyster
               reef was extensive and the outer margin of the reef was marked with four wood
               pilings. However, in Year II & III, no oysters could be found in the subtidal
               sampling area and the site was moved to the shoreline where a small bayou
               enters the bay (southeast from a large white house) at latitude 30*24.35'N and
               longitude 86'12.75'W. Oysters were attached to rocks and a wooden bulwarks
               around a large tree. Oysters were small and not overly abundant. The site was
               moved in Year IV due to oyster depletion at the shoreline site. In Years IV, V
               and VI, oysters were tonged southeast of channel marker 40 (30'24.53N,
               86011.40'W).

               OYSTER COLLECTIONS

               Year I - The oyster reefs south of channel marker 34 were tonged for oysters. This
                     site was approximately 1 mile west of the drawbridge and 1 mile north of
                     the south shoreline. Oyster stations were located on a south to north line
                     with Station 1 being on the south end. Station 1 was approximately 300 m
                     south of channel marker 34 on a bearing of 250'. Other bearings to locate
                     the station were: 300' from the south end of the drawbridge, 108' from
                     south Live Oak Point, and 350' from the microwave tower. Station 2 was
                     approximately 100 m to the north and Station 3 was another 100 m to the
                     north near the channel marker.

               Year II - No oysters could be located in the area sampled during Year I, so the
                     site was move to the shoreline. To reach the shore site, take a bearing of 70'
                     from channel marker 34 to a small bayou. This site was southeast from a
                     large white house on the shore. Oysters were attached to wood and rocks
                     along the bank. No significant geographic distinction could be made
                     between the oyster stations because of the paucity of oysters.

               Year III - Oysters were again collected from the rocks and wood pilings. Stations
                     were segregated with 50 meters between locations.




                                                       405









              Year rV - In Year IV, the site was relocated to a commercially fished reef near
                    channel marker 40 (bearing 062* to the marker). The reef ran northeast-
                    southwest (260/0800) and was marked by poles and signs "Danger,
                    submerged oyster reefs". This reef was in 3 m of water and had to be
                    sampled by tonging. Station 1 was at the southern end of the reef about 50
                    m north of the last pole. Station 2 was 30 m south of the "Danger..." sign,
                    near the middle of the reef. Station 3 was at the northeast end of the reef,
                    bearing 240' from channel marker 40, and 15 meters west of the northern
                    most pole marking the reef.

              Year V - Stations were located in approximately the same locations as last year.
                    Oysters were sparse due to recent die-offs, and many recently dead oysters
                    were tonged. Station 1 was 30 m south of the southmost pole on the west
                    side of the reef, between the two poles marking the south end of the reef.
                    Station 2 was in the middle of the reef, 15 m south of the second pole from
                    the north. Station 3 was at the northeast end of the reef, between 15 and 50
                    m west of the northmost pole.

              Year V7 - Stations were in the same locations as last year. Oysters were dredged
                    and were more abundant this year.

              Years M & VIII - Oysters were dredged from the same stations sampled the
                    previous three years. Oysters were abundant both years but were covered
                    with numerous mussels.

              Year IX - The site was not scheduled to be sampled this year.

                 )MEW COLLEMONS

              Year I - Sediment samples in Year I were collocated within 30 m of each oyster
                    statiom These sediments were a charcoal gray to black mud overlain by
                    dark brown surface.

              Year H - Sediments sampled were a loose black mud taken on a line from the
                    oyster collection site on shore toward channel marker 34. Station 1 was 500
                    m from channel marker 34 marker with Stations 2 and 3 being 400 and 300
                    m from the marker. Sediments shoreward of this point (nearer the oysters)
                    were much too sandy.

              Year III - Sediment stations in Year III were collected on the same line at
                    distances of 100 m apart on a line to channel marker 34.

              Year IV - No sediments were sampled.

              Year V - Sediment samples were black silty sand mixed with oyster shell and
                    were collected near each oyster station. Station 1 was collected 30 m north
                    of the pole at the oyster station. Station 2 was 30 m north of the pole at the
                    oyster station. Station 3 was also 30 m north of the pole at that oyster
                    station.



                                                      406









                Year VI - Sediments (NIST) were collected adjacent to each oyster station.

                Years VII, VIII, & IX - No sediments samples were collected during these years.

                SAMPLING METHOD

                       Oysters - tongs and hand
                       Sediments - box core

                WATER DEPTH - subtidal, 2 - 3 meters (Years I, IV, V, VI) and intertidal, 0 - 0.3
                (Years II & III)

                POSSIBLE CONTAM[INANTS - No sources of contamination were evident in the
                area.


                ENVIERONMENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (%0)                  (OC)

                      YEAR I                  -                   14.0           2 March 1986
                      YEAR 11                 5.0                 12.0          26 February 1987
                      YEAR 111                6.0                 11.0          17 February 1988
                      YEAR IV               21.0                  15.0          26 January 1989
                      YEAR V                  4.0                 15.0          27 January 1990
                      YEAR VI               12.0                  10.8          27 January 1991
                      YEAR VII              21.0                  11.0          22 January 1992
                      YEAR VIII             10.0                  16.7          11 January 1993





















                                                            407












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                                                                               Site #38 (CBSR), Choctawhatchee Bay Off Santa Rosa.
   DDeL40rme Mapping COmPanY. Map Taken from Florida Atlas and Gazetteer. Reproduced with permission.









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                                                                                        Site #38 (CBSR), Choctawhatchee Bay Off Santa Ro8a (from chart 11385).




















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              SITE NUMBER 82                                 DESIGNATOR - CBBL

              SITE - BEN'S LAKE, CHOCTAWHATCHEE BAY, FL

              NONIINAL SITE CENTER - 30027.15'N              86032.45rW

              LOCATED ON NOS CHART             11388,11385

              SITE ACCESS - The site is reached by boat with access from the boat ramp located
              on Bayshore Drive on the east side of Boggy Bayou. Travel from Fort Walton to
              Niceville on Highway 85, cross Boggy Bayou bridge, turn right on Bayshore Drive,
              and continue to the public ramp on the right. By boat go south down Boggy Bayou
              to Choctawhatchee Bay, then go west to the entrance to Bens Lake. Travel time is
              approximately 30 minutes in good weather.

              SITE DESCRIPTION - This site, established in Year VII, was located at the
              entrance to Bens Lake which was on the north side of Choctawhatchee Bay. The
              entire area was part of the Eglin Air Force Base. Oysters were located on concrete
              and brick rubble along the shoreline at the entrance to Bens Lake.

              OYSTER COLLECTIONS

              Year VII - Oysters were collected by hand from concrete, brick and rocks in the
                    intertidal zone. Station 1 was from the east side of the entrance into Bens
                    Lake. Station 2 was also on the east side of the entrance into the bay but was
                    30 m to the north. Station 3 was on the west side of the entrance into the
                    bay.

              Year VIII - This site was not visited this year.

              Year IX - Oysters were collected from the same station locations as in Year VII.

              SEDDIENT COLLECTIONS


              Year VII - Sediment collection sites were located within Bens Lake. Station 1
                    was near the east bank approximately 150 m from the entrance. Station 2
                    was also on the east bank, approximately 75 m from the entrance. Station 3
                    was near the marina pier on the west bank of the lake.

              Years VIII & IX - No sediments were collected during these years.

              SAMPLING M[ETHOD

                    Oysters - hand
                    Sediment - box corer

              WATER DEPTH - intertidal, 0.1 to 0.5 meter





                                                      413









           POSSIUBLE CONTAMINANTS - A possible source of contamination was surface
           runoff originating from the marina area.

           ENVIRONAUMAL DATA

                                 Salinity      Temperature     Dates Sampled
                                  (9010)           (0c)

                YEAR VII          20.0             14.0       23 January 1992
                YEAR IX           25.0             13.0       17 January 1994








































                                               414











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                                                                           Site #82 (CBBL), Chodawhatchee Bay Bens Lake


     ODeLorme Mapping Company. Map Taken from Florida Atlas and Gazetteer. Reproduced with permission.


















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                                                                 Site #82 (CBBL), Choetawhatchee Bay Ben












                                                                                          il 14










              SrIE NUMBER 39                                 DESIGNATOR - CBPP

              SITE - POSTIL POINT, CHOCTAWHATCHEE BAYI FL

              NONIINAL SITE CENTER - 30028.85'N              86028.73V

              LOCATED ON NOS CHART            11388,11385

              SITE ACCESS - The site is reached by automobile with access through Eglin Air
              Force Base. Enter Eglin Air Force Base on Highway 85. Stop at the visitor center
              at Eglin Air Force Base and obtain a pass and ask the guard to direct you to the
              family camping area at Postil Point.

              SITE DESCRIPTION - The site was located on the west bank at the entrance to
              Boggy Bayou which was on the north side of Choctawhatchee Bay. A military
              Family Camping and Recreation area was located on Boggy Point. Oysters were
              collected by hand from along the shoreline from the entrance into Postil Lake
              north to Postil Point.


              OYSTER COLLECTIONS

              Years I, II, III, & IV - Oysters were collected by hand from intertidal waters.
                    Station 1 oysters were taken from sandy bottom, jetty rocks and the
                    bulkhead where Postil Lake entered Choctawhatchee Bay. Oyster Station 2
                    was located along the shoreline -200 m north of Station 1. Oysters were
                    attached to the rocks. Oyster Station 3 was on the south side of Postil Point
                    in the shoreline rocks. Oysters were not overly abundant at this site.

              Year  V - Oysters were collected at the same sites as were previously sampled.
                    This year there were fewer oysters due to the recent freeze. All of the
                    oysters above the low tide mark were recently dead.

              Year VI - Oyster sampling stations were located in the same areas as previously
                    sampled. Oysters were more abundant than last year.

              Year VII - Oyster sampling stations were located in the same areas as previously
                    sampled. Oysters were less abundant this year.

              Year VIII - Oysters were collected at the same stations described for Year I.

              Year IX - The oyster sampling stations were the same as those used in Year I.
                    The population was sparse as many oysters had been killed in a recent cold
                    weather freeze.

                   I M[ENT COLLECTIONS

              Year I - Sediments were collected in Choctawhatchee Bay out from Postil Point.
                    These sediments were primarily sand.




                                                      419










               Years II & III - Sediments were collected along the northwest shoreline in Postil
                      Lake. Sediment Station 1 was -25 m north of the entrance into the lake
                      near oyster Station 1. Station 2 was -50 m north of Station 1. Station 3 was
                      another 50 m to the north along the shoreline. Sediments were primarily
                      organic peat with some silt and were characterized by a strong H2S odor.

               Year IV - Sediments were not collected this year.

               Year V - Sediments were co      flected ffom the same stations as in Years II & III.

               Years VI, VII, VIII, & IX - No sediments were sampled during these years.

               SAMPLING METHOD

                      Oysters - hand
                      Sediment - hand

               WATER DEPTH - intertidal, 0. 1 to 0.2 meter

               POSSIBLE CONTAMINANTS - A possible source of contamination was from
               surface runoff originating from the recreational area.

               ENVIRONMENTAL DATA

                                          Salinity           Temperature         Dates Sampled
                                            (10/00)               (0c)

                    YEAR 1                  13.3                  16.0             1 March 1986
                    YEAR 11                   8.0                 13.0           26 February 1987
                    YEAR 111                20.0                    9.0          17 February 1988
                    YEAR IV                 18.0                  18.0           26 January 1989
                    YEAR V                    5.5                 17.0           27 January 1990
                    YEAR VI                 17.3                  13.7           27 January 1991
                    YEAR VII                18.3                  14.0           23 January 1992
                    YEAR VIII               10.0                  17.0           11 January 1993
                    YEAR IX                 .24.0                   6.0          16 January 1994














                                                            420


















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                                                                    Site #39 (CBPP), Choctawhatchee Bay Postil Point (from chart 11385).

















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               SITE NUMBER 83                                 DESIGNATOR - CBBB

               SITE - BOGGY BAYOU, CHOCTAWHATCHEE BAY, FL

               NOXINAL SITE CENTER - 30030.18'N               86029.65W

               IA)CATED ON NOS CHART # - 11388,11385

               SITE ACCESS - The site is reached by boat with access from the boat ramp located
               on Bayshore Drive on the east side of Boggy Bayou. Travel from Fort Walton to
               Niceville on Highway 85, cross Boggy Bayou bridge, turn right on Bayshore Drive,
               and continue to the public ramp on the right. By boat go south down Boggy Bayou
               to channel marker 9, turn southwest and proceed into Toms Bayou. Proceed west
               for approximately 500 m to the bridge.

               SITE DESCRIPTION - This site was established in Year VII. The oysters were
               located on the piling of the bridge which crossed the middle of Toms Bayou.
               Station 1 was on the south end of the bridge and includes the first 5 sets of bridge
               pilings. Station 2 included the middle 5. sets of piers. Station 3 was composed of
               the northern 5 sets of piers.

               OYSTER CO1,LECTIONS

               Year VII - Oysters were collected from the concrete bridge pilings. Station 1 was
                     on the south end of the bridge and includes the first 5 sets of bridge pilings.
                     Station 2 included the middle 5 sets of piers. Station 3 was composed of the
                     northern 5 sets of piers.

               Year VIII - Oysters were not collected at this station this year.

               Year IX - The oysters were easily collected from the bridge pilings described in
                     Year VII. Small to medium sized oysters were numerous at all of the
                     stations.


               SEDDIENT COLLECTIONS


               Year VII - Sediments were collected in 1 to 2 m of water from the base of the
                     pilings at each of the three oyster collection stations. Sediments were softy
                     silty brown mud over a hard sandy layer.

               Year VIII & IX - Sediment samples were not taken during these years.

               SAMPLING METHOD

                     Oysters - hand
                     Sediment - box corer

               WATER DEPTH - intertidal, 0.5 meter




                                                        425









            POSSIBLE CONTAMINANTS - A possible source of contamination was from
            surface runoff originating from Valparaiso and Eglin Air Force Base.

            ENVIRONMENTAL DATA

                                Salinity       Temperature    Dates Sampled
                                  (%0)             (0c)

                YEAR VII          18.0             14.0       23 January 1992
                YEAR IX           17.0             7.0        16 January 1994








































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                                                                                                                                                                        "6"                                                                                   3
                                          Racing buoys within the limits of this chart                                                                                 19                                                          A>
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                                        obtained from the U.S. Coast Guard Distri                                                                                10 17        21         1                                                                    4oft
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               SrIE NUMBER - 74                              DESIGNATOR - PCLO

               SITE - LITTLE OYSTER, PANAMA CITY

               NOAM,AL SITE CENTER - 30015.19'N               85040.95'W

               LOCATED ON NOS CHART            11390

               SITE ACCESS - Access to the site is via boat launched at the public ramp at
               Richard Simpson Park. The park is located at the east end of the Hathaway
               Bridge (Highway 98) on the north side where it crosses West Bay. Cross the bridge
               then turn north on Moody Avenue to the old highway. Turn left and the ramps
               are on the right. In Year VII the boat was launched at the Leslie Port Wayside
               Park boat ramp which in on Highway 77 on the north side of Panama City.

               SITE DESCRIPTION - The site was not established until Year IV of the program
               and was located at Little Oyster Bar Point in North Bay. The site was on the edge
               of the U.S. Air Force Petroleum Depot. The oyster and sediment sites were
               separated across the bay by a distance of about 2 miles. The sediment site was
               located near the mouth of Mud Bayou and east of West Bay Point.

               OYSTER COLLECTIONS

               Years IV & V - Oysters were taken from the concrete bases of the high voltage
                     transmission towers crossing the bay at Little Oyster Bar Point. Station 1
                     was on the tower on the shoreline. Station 2 was the next tower from the
                     east shore (southeast of channel marker 6). Station 3 was the second tower
                     from the east shore (north of channel marker 6). Station 1 could be collected
                     as a walk-up station, but the other two stations had to be sampled from the
                     boat.

               Year  VI - All three stations were collected at the same locations previously
                     sampled. Station 1 was depleted of oysters and will have to be moved next
                     year. This was partially due to siltation occurring around the base of the
                     concrete pilings and at the mouth of the small pond which blocks the tidal
                     flow at this site.

               Year  VII - Station 1 was completely silted over and no oysters were present;
                     therefore, it was moved to the northwest side of the bay. The new site was
                     established at the second set of transmission piers out from the north
                     shoreline. The new site was located at 30"15.59 north and 85'41.13 west.
                     Stations 2 and 3 remained at the locations previously sampled.

               Year VIII - Collection stations remained the same as described in Year       Vii.

               Year IX - The previosly sampled stations could not be reached, due to adverse
                     weather conditions. Stations 1 & 2 were collected 50 m east of the old Station
                     1 (Years IV & V). The site was a small intertidal pond next to the


                                                       431









                      shoreline. Station 1 & 2 oysters were contiguous to one another. Station 3
                      oysters were collected from the electrical transmission pilings on the
                      northshore side of the bay. Oysters at both sites were all very small and
                      sparsely situated.

                   )UMEM COLLECTIONS

               Years IV & V - Sediments were collected at the mouth of Mud Bayou (30'14.27'N
                      and 85' 42.69'W). Station 1 was to the north of the Bayou. Station 2 was just
                      to the south of the mouth at the sandy beach. Station 3 was 300 meters
                      south of the Bayou mouth. All stations were sampled in 0.2 meters of water
                      approximately 25 meters from the shoreline. There were oysters scattered
                      in the grass beds and several small reefs in this area but oysters were too
                      few and scattered to be reliable for repeat sampling.

               Years V1, VII, VIII, & IX - Sediments were not sampled during these years.

               SAMPLING AffivMODS

                      Oysters - hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0 to 0.3 meters.

               POSS11BLE CONTAMINANTS - Possible contamination included a military fuel
               depot at Little Oyster Bar Point and a coal burning power plant across the bay.

               ENVIRONNEF,NTAL DATA

                                         Salinity           Temperature         Dates Sampled
                                            (%0)                 (0c)

                    YEAR IV                 30.3                 20.0           27 January 1989
                    YEAR V                  14.0                 15.0           28 January 1990
                    YEAR VI                 18.3                 13.2           28 January 1991
                    YEAR V11                25.0                 14.0           24 January 1992
                    YEAR VIII               18.7                 19.0           12 January 1993
                    YEAR IX                 10.0                 12.0           17 January 1994














                                                           432













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               SHE NUMBER 68                                   DESIGNATOR - PCMP

               SITE - MUNICIPAL PIER, PANAMA CITY, FL

               NONONAL SITE CENTER - 30009.00'N                85039.80V

               LOCATED ON NOS CELART # - 11390

               SITE ACCESS - This station is located at the Bay City Marina in Panama City. A
               boat is necessary to collect this site and is launched at the public boat ramp. All
               three stations are located at the marina.


               SITE DESCRIPTION - This site was not established until Year III and was
               located at the Bay City Marina. The stations were placed inside and at the
               entrance of the marina.


               OYSTER COLLECTIONS

               Year  III - Station 1 for both oysters and sediments was located inside the
                     breakwater on the east side of the marina. Oysters were collected along the
                     breakwater and were numerous at and to 0.5 m below the normal high tide
                     line. Station 2 was located opposite Station 1 on the outside of the
                     breakwater. Oysters 'Were slightly less abundant but were still numerous.
                     Oyster Station 3 was located north of Station 2 and outside the breakwater.
                     It was directly east of the parking lot which was north of the boat ramp.
                     Oysters were collected from a submerged concrete mass but were also
                     found below the tide line of the breakwater.

               Years IV & V - In both years, the collection sites were the same as was first
                     sampled in Year III. Station 1 was inside the harbor on the bulkhead
                     directly southeast of the boat ramp. Station 2 was outside the marina,
                     directly opposite Station 1. Station 3 was directly north of Station 2 along the
                     concrete bulkhead next to the parking lot at the boat ramp.

               Years VI & VII - Oysters were not sampled at this station during these years.

               Year VIII - Oysters were collected at the stations described for Year III.

               Year LX -The site was not scheduled for collection this year.

               SEDUvMW COLLECTIONS

               Year III - The only fine grained sediments were at the base of the bulkhead at
                     Station 1. No suitable sediments were found in the vicinity of Stations 2 and
                     3, nor in any location near the marina.

               Years IV & VI - No sediment samples were collected during these years.

               Year V - Sediment samples were collocated with each of the three oyster stations.



                                                        437










               Years VII, VIII, & IX - Sediments were not sampled during these years.

               SAMPLINGMETHODS

                      Oysters - tongs and by hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0 to 0.3 meter

               POSSIBLE CONTAMINANTS - Potential sources of contamination were likely
               from the boat traffic in the marina as well as run-off from the nearby streets of
               downtown Panama City.

               ENVIRONADMWAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (9010)               (OC)

                    'YEAR 111               33.0                   9.0         18 February 1988
                    YEAR IV                 33.3                 16.0           27 January 1989
                    YEAR V                  24.0                 15.0           27 January 1990
                    YEAR VIII               20.0                 19.3           12 January 1993




























                                                           438










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               SITE NUM33ER 40                                 DESIGNATOR - SAWB

               SITE - WATSON BAYOU, ST. ANDREW BAY, FL

               NONONAL SITE CENTER - 300 08.53'N               85037.92'W


               LORAN TD's - 14142.0             46990.4

               LOCATED ON NOS CHART             11389,11390

               SITE ACCESS - The station is located in Watson Bayou which is located east of the
               Bay City Marina in Panama City. After collection of the Municipal Pier site travel
               by east down the ICWW to Watson Bayou. The site is at the first point on the right
               (east side) after entering the Bayou.

               SITE DESCRIPTION - The oysters were attached to rocks on a point of land
               identified by a former shipyard, just west of the dockage for ships and boats on the
               channel that led up to the paper mill. Oysters were intertidal, small and sparse.

               OYSTER COLLECTIONS

               Years I, II, & III - All the oysters were collected at the same location in Years I,
                     II and III. No significant geographic distinction could be made between
                     oyster stations. Landmarks to locate the site were: 352' to the white oil
                     tanks; 78' to the tall smoke stack.

               Years rV, V, VI, VII, & VIII - The site was the same as in previous years. All
                     stations were taken from the rock rubble along the shore on the first point
                     on the east side of Watson Bayou.

               Year IX - The site was not  scheduled to be visited this year.

                  DINIENT COLLECTIONS

               Years I, II, & III - Sediments in the entire bay area were coarse sands, except for
                     the upper reaches of the slough leading to the paper mill. Sediments were
                     sampled just south of the small bridge at the paper mill (Station 1), across
                     the channel from the paper mill (Station 3), and slightly upstream (near the
                     outfall pipe) from the paper mill near the upper end of the slough (Station
                     2). The sediments were a black ooze with the consistency of pudding.
                     Sediments and the air had a peculiar and unpleasant odor.

               Years IV & VI - No sediment samples were collected.

               Year V - Sediment samples were in the same locations as sampled during the
                     first three years.

               Years VII, VIII, & IX - Sediments were not sampled during these years.



                                                        443











               SAMPLING METHOD

                      Oysters - hand
                      Sediments - box core

               WATER DEPTH - intertidal, 0 - 0.5 meters

               POSSIBLE CONTAMINANTS - Probable contamination would be expected from
               the dockage facilities around the comer from the site, fuel oil storage and transfer
               facilities around the embayment, and the effluents from the paper mill.

               ENVIRONMENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (%0)                 (0c)

                    YEAR 1                  23.0                 15.0            1 March 1986
                    YEAR 11                 18.0                 14.0           25 February 1987
                    YEAR 111                33.0                   9.0          18 February 1988
                    YEAR IV                 32.0                 17.0           27 January 1989
                    YEAR V                  20.0                 17.0           28 January 1990
                    YEAR VI                 14.0                 14.0           28 January 1991
                    YEAR VII                24.0                 14.0           24 January 1992
                    YEAR VIII               20.0                 18.0           12 January 1993




























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               SITE NUMER 41                                  DESIGNATOR - APDB

               SITE - DRY BAR, APALACHICOLA BAY, FL

               NON[INAL SITE CENTER - 29040.45'N              85004.40W

               LOCATED ON NOS CHART # - 114022 11404

               SITE ACCESS - The site is accessed by a 30 minute boat ride originating at the
               ramp in Apalachicola near the southwest end of the John Gorrie Memorial
               Bridge. Proceed west along the inside channel to the first channel going south
               into Apalachicola Bay. Run a compass course (bearing 2500) to the northeast end
               of St. Vincent Island at St. Vincent Point.

               SITE DESCRIPTION - The site was located on the northeast corner of St. Vincent
               Island at St. Vincent Point. Oysters were located north of the point on subtidal
               shell and sand bottom.


               OYSTER COLLECTIONS

               Year I - Stations were located in a line running south to north. Station 3 was at
                     bearing of 330' from St. Vincent Point, 105' from Green Point and 60' from
                     the Apalachicola Bay drawbridge.            During Year I stations were
                     submerged.

               Year  II - Year II samples were collected by hand from an abundant oyster
                     population. Stations were oriented in a west to east direction paralleling St.
                     Vincent Point. Station 1 was located on the west end of the bar, Station 2
                     was 200 m east of Station 1, and Station 3 was 200 m farther east.

               Year III - Year III oysters were collected at the same sites as Year II, but the
                     oysters were tonged due to high tidal conditions.

               Years IV, V, VI, VII, & VIII - Oysters were collected by hand at the same
                     location described for Year II.

               Year IX - The site was not scheduled for collection this year.

               SEDOWNT COLLECTIONS

               Year I - Sediments were obtained from one location 30 m to the east of oyster
                     Station 3.

               Year II - No sediments of an appropriately fine texture were found anywhere
                     near the oyster site. Sediments were taken from the mouth of the
                     Apalachicola River near St. Vincent Island which is south of the bridge.

               Years III, IV, V, VT VIT VIII, & IX - Sediments were not sampled during these
                     years.




                                                       449











               SAMPUNGMETHOD

                      Oysters - tongs and by hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0 to 1 meter

               POSSIBLE CONTAMINANTS - Contamination factors included the river, rusty
               iron dump near samples, and ship traffic.

               ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                 (OC)

                    YEAR 1                 13.0                 17.0           28 February 1986
                    YEAR     11              7.6                17.0           27 February 1987
                    YEAR     111           17.0                 12.0           18 February 1988
                    YEAR     IV            20.0                 18.0           28 January 1989
                    YEAR V                   3.0                16.0           27 January 1990
                    YEAR VI                10.0                 13.0           28 January 1991
                    YEAR VII               15.0                 13.0           24 January 1992
                    YEAR     VIII            5.0                20.0           13 January 1993




























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               SITE NUMBER 42                                  DESIGNATOR - APCP

               SITE - CAT PT. BAR, APALACHICOLA BAY, FL

               NONIINAL SITE CENTER - 29043.45'N               84053.05'W

               LOCATED ON NOS CHART # - 11404,11402

               SITE ACCESS - This is a walk-up site from the beach at low tide. It is accessed by
               boat when tides are high. To reach the site drive south on road GlA to the toll
               booth. Turn left on the dirt road to the east to East Point Beach. Park and walk to
               East Point Beach where oysters can be picked up by hand at low tide. If the tide is
               high the boat can be launched at one of the ramps on Highway 30, which is east of
               the site.

               SITE DESCRIPTION - The site was located at Cat Point. The reef was exposed at
               low tide and was the major source for oysters in eastern Apalachicola Bay. At
               high tide the oysters were collected by tonging.

               OYSTER COLLECTIONS

               Year I - Oysters were collected by hand along the exposed reef. Station 1 was 100
                     m off the beach, south of East Point. Station 2 was located 100 m south of
                     Station 1 -200 m offshore. Station 3 was east of Stations 1 and 2 -25 m
                     offshore.

               Year 11 - During Year II, the boat was. launched from the ramp in the boat basin
                     at the town of East Point (two blocks south of Highway 98). It was a five
                     minute ride to the tonging site on a large reef that parallels the telephone
                     poles crossing the bay. The oyster site was 200 m east of telephone pole #6
                     (bearing 260' to sixth pole from shore). Oysters were abundant but tonging
                     was required; dredges were not permitted in Apalachicola Bay. Oysters
                     were collected for all stations at one place in Year II.

               Years III, IV, V, & VI - Samples were taken at the Year II station locations.
                     However, since the tide was low, the oysters were collected by hand and
                     separate stations were established. Oysters stations were -100 m apart.
                     Station 1 was at the north end of the reef, Station 2 in the middle and Station
                     3 was at the south end.

               Years VII & VIII - Samples were taken at the same locations described for Year
                     III.

               Year IX - The site was not scheduled to be visited this year.

               SEDIMENT COLLECTIONS







                                                        455









               Year I - Sediments for the first year were located just off the reef (east and
                      northeast) collocated with each oyster station. The sandy mud sediments
                      were as fine a texture as could be found in the area.

               Year II - Sediment Station 1 was located 500 m east of #6 pole. Station 2 was 500 M
                      east of the 8th pole. Station 3 was 500 ni east of the 4th pole. The substrate
                      was sandy mud with small shell fragments.

               Year III - Sediments were collected 500 m east of the oyster stations. Sediments
                      were fine silt over sand.

               Years IV, V, VI, V11, VIII, & IX - Sediments were not sampled during these
                      years.

               SAMPLINGMETHOD

                      Oysters - tongs and by hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0 to 0.7 meters

               POSSIBLE CONTAAHNANTS - No sources of contamination were evident.


               ENVIRONMENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (%0)                  (OC)

                    YEAR 1                    7.0                 18.0          27 February 1986
                    YEAR     11             30.0                  15.0          26 February 1987
                    YEAR     111            15.0                  12.0          18 February 1988
                    YEAR     IV             33.0                  12.0          28 January 1989
                    YEAR V                    9.0                 20.0          28 January 1990
                    YEAR VI                 22.3                  14.0          28 January 1991
                    YEAR VII                11.3                  11.0          24 January 1992
                    YEAR     VIII           20.0                  19.0          13 January 1993
















                                                           456




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                                                           Site #42 (APCP), Apalachicola Bay Cat Point Bar.

  ODeLorme Mapping Company. Map Taken from Florida Atlas and Gazetteer. Reproduced with permission.













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               SrIE NUMBER 75                                  DESIGNATOR-AESP

               SITE - SPRING CREEK, APALACHEE BAY, FL

               NONEINAL SITE CENTER - 30003.75'N             84019.37V

               LOCATED ON NOS CHART # - 11405

               SM ACCESS - To reach the site turn off of U.S. Highway 98 onto 375 and proceed
               to Highway 365. Turn right and go to the end of the road. The Spears Seafood
               Company at the end of the road has a launch that can be used. Proceed out the
               marked channel to the collection site.

               SITE DESCRIPTION - This site, which was established in Year IV, was located
               on the west side of Apalachee Bay where Spring Creek enters Oyster Bay.
               Sampling stations were located in the midst of very prolific reefs where Spring
               Creek entered Oyster Bay. The site was located along an "S"-shaped reef 300 m
               south of channel.markers 31 and 32, and northwest to southwest of channel
               markers 29 and 30.


               OYSTER COLLECTIONS

               Year IV - Year IV oysters were sampled by hand from the intertidal parts of the
                     reef. Station 1 was at the north end of the exposed part of the reef which
                     extends as a subtidal portion farther north into the marsh. Station 3 was at
                     the south end of this reef. Station 2 was located on the east side of the reef,
                     mid-way between 1 and 3. Reef length was 300 m.

               Year V - Collection locations were the same as established last year. The number
                     of oysters were much reduced from last year. Most of the reef was
                     composed of dead shells.

               Year VI - Station locations were the same as last year. Oysters were more
                     abundant than last year.

               Years VII, VIII, & IX - Station locations were at the same sites as originally
                     established in Year IV.


                   1 1M]KW COLLECTIONS

               Year rV - Sediments for Station 1 were taken about 100 m from the reef, midway
                     and on a line from the north end of the reef to channel marker 29. Stations
                     2 and 3 sediments were located adjacent to the oyster stations about 25 and
                     50 m, respectively, from each station on the east side of the reef.

               Year V - Sediments were collected adjacent to each oyster station. Station 1 was
                     from the west side of the reef 10 m from the shoreline. Station 2 was also on
                     the west side of the reef 10 m from the shore adjacent to the oyster station.



                                                        461









                     Station 3 was 5 m from the reef on the west side. Sediments were composed
                     of fine grained black silt in pockets over shell and shell hash.

              Years VI, VII, VIII, & IX - Sediments were not sampled during these years.

              SAMPUNG METHODS

                     Oysters - hand
                     Sediment - box core

              WATER DEPTH - intertidal, 0 - 0.5 m.

              POSSIBLE CONTAMINANTS - There were no obvious contamination sources,
              although the site was located in waters which were closed and/or conditional to
              shellfish harvesting.

              ENVIRONMURNTAL DATA

                                        Salinity          Temperature         Dates Sampled
                                           (0/00)              (0c)

                   YEAR IV                 15.7                17.0           28 January 1989
                   YEAR V                   8.3                19.0           29 January 1990
                   YEAR VI                  1.0                15.0           29 January 1991
                   YEAR VII                22.0                15.0           30 January 1992
                   YEAR VIII                5.0                20.0           13 January 1993
                   YEAR EK                 13.3                15.0           18 January 1994

























                                                         462












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               SITE NUMBER 69                                 DESIGNATOR - SRVVT

               SITE - WEST PASS, SUWANNEE RIVER, FL

               NOXONAL SITE CENTER - 29019.75'N               83010.45W

               LORAN TD's - NA


               LOCATED ON NOS CHART            11408

               SITE ACCESS - Established in Year III, this site is accessible only by small boat.
               Access is from the Suwannee Marina located near the middle of the town. From
               the boat launch proceed southwest to the first channel to the north. Proceed
               slowly up the channel which passes through Suwannee. The channel passes
               under a bridge then bends left, passes under another bridge and then turns left
               and then right. Turn north at the last house on the right into another channel
               and proceed until it intersects a channel with markers. Proceed out to channel
               marker 20 and go north for -2 km to Cat Island.

               SITE DESCRIPTION - This site was established and sampled only in Year III.
               Oysters were collected along the margins of Cat Island. Reefs were numerous
               east of Cat Island and were exposed at low tide. The margins of the islands were
               covered with Spartina alterniflora.

               OYSTER COLLECTIONS

               Year  III - Oyster Station 1 was located on the northeast shore of Cat Island
                     between a topless palm and a pole in the water. At low tide the reef is
                     exposed and numerous small oysters are present. Oysters can be collected
                     by hand or tonged. Station 2 was a subtidal reef with an exposed intertidal
                     portion. It was located between Cat Island and a small island to the
                     northeast. Station 3 was -50 m north of Station 1 on the north side of the
                     same reef. Oysters at all three stations could be collected by hand at low tide
                     but had to be tonged at high tide.

               Years IV, V, VI, VII, VIII, & IX - The site was not sampled during these years.

               SEDIMENT COLLECTIONS

               Year III - Sediment Station 1 was collected along the intertidal marsh on the east
                     side of Cat Island. Station 2 was in a small inlet directly east of the second
                     oyster station. Sediment was a peaty/organic sandy mud. Station 3 was
                     collected several hundred meters away from the other station. This station
                     was collected when returning to the ramp, after a turn north at channel
                     marker 22 into a cove that ran west from the main channel. A sawgrass
                     marsh surrounded this site and the sediments were an organic peaty mud.

               Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                     years.




                                                       467










            SAMPLING METHODS

                 Oysters - tongs and by hand
                 Sediment - box core

            WATER DEPTH - intertidal, 0 - 1 meter

            POSSEBLE CONTAMINANTS - No contaminant sources were identified at this
            site.


            ENVIRONMENTAL DATA

                                 Salinity      Temperature     Dates Sampled
                                  (%0)             (0c)

                YEAR 111           13.0            15.0       19 February 1988


































                                               468


















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                                                  Site #69 (SRV;P), Suwanee River West Pass.



      CDeLAxme Mapping Company. Map Taken from Florida Atlas and Gazetteer, Reproduced with pemiission.












                                                                                                                                                HyarogrOpny ono t0P0Y1UIJ1iY uY L-00
                                                                                                                                                                          ervices with additional data from the Corps
                                       2                                                                                                       horting and Geodetic S
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                       3                 31                                                   or                                              of Engineers. Geological Survey and U.S. Coast Guard.
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                                       4                                             2                                                              Guard Light List and U.S, Coast Pifol for details.
                                                                           3
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                                                                                     2                                        M                          For Symbol$ and Abbreviations W Chart No. I
                                                                                                                               /   '114 COLREGS:         international Regulations for Preventing Collisions at Sea,       19
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                                                                                     0         me
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                                                                                                                                                                                        SUWANEE RIVER
                                             67              h             3
                                                                                                                                                                     The controlling centerlIne depth was 31, feM from a
                                                                                                                                                                   at 290 1 91001N; 83*071061W to Fanning; thence 3,1
                                                       3                               2                 4                                                         Ellaville.
                                                   5                                                                                                                                                               May-July
                                                 9                                     2               10
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                                                                                     A                                 CFO                                         Island and Ellaville. MINIMUM APPROX VERT
                                                               7                          3      3           0                                                     CL. 35 FT. SWING BRIDGES, FIXED BRIDGES
                            5                                                                  *be*                                            M                   BRIDGES UNDER CONSTRUCTION between Bra
                                                                           64        '--.1    1                                  q               Orah              Island and Ellaville. MINIMUM HOR. CL. 48
                          7 13      7      7            7                            5            4     3                                                          MINIMUM VERT. CL.         5 FT.

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                                                                           Site #69 (SRWP), Suwanee River West Pass (from chart 11408).


































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                                                                                            39











              SrIE NUMBER 43                                DESIGNATOR - CKBP

              SITE - BLACK POINT, CEDAR REY, FL

              NONIINAL SITE CENTER - 29-12.32'N             83004.25rW


              WCATED ON NOS CHART # - 11408

              SITE ACCESS - Access to the site in Years I and 11 was by boat launched at the
              city boat basin at the end of Highway 24 in Cedar Key. Shoals require that the
              channel markers be followed from the city marina to Derreck Key. The oyster site
              is located on the northeast side of a "U" shaped island adjacent to channel marker
              2 at Derreck Key. Approximate run time to the site is an hour. In Year III, the
              boat was launched at a boat ramp north of Cedar Key at the end of Highway 326 off
              of Highway 347. The ramp is very shallow and launching at low tide is difficult,
              but the site can be reached in less than 10 minutes. From the boat ramp go south
              - 1 km.

              SITE DESCRIPTION - Oysters were collected from the subtidal perimeter of the
              small island. At low tide there were numerous exposed oyster reefs. The oyster
              site was located at a crescent-shaped reef with smooth cordgrass on the west side,
              west of an island with three palm trees and one topless palm. The stations were
              located inside the crescent.

              OYSTERCOLLECTIONS

              Year I - Year I oysters were collected from the perimeter of the small island in
                    subtidal and intertidal water. Station 1 was at the mouth of a creek 0.75
                    miles from Black Point on a bearing of 125'. Other bearings to locate the
                    site were 245' from the radio tower, 285' from the bridge center, and 345'
                    from the water tower. Station 2 was an exposed reef 220' from Station 1.
                    Local landmarks had a bearing of 2500 from the radio tower, 284' from the
                    bridge and 350' from the water tower. Station 3 was 300 m from Station 1 on
                    a bearing of 120'.

              Year II - Significant geographic differentiation of the oyster stations was not
                    possible this year. Bearings to local landmarks were 262' to channel
                    marker 2, 149" to the Cedar Key water tower, and 317' to green channel
                    marker 5. The oysters were collected by hand from subtidal and intertidal
                    clumps which made up the island.

              Year  III - In Year III, samples were collected in approximately the same
                    location as Year II. The site was a crescent shaped reef covered with
                    smooth cordgrass. An obvious landmark to locate the site was the three
                    palm trees due east of the site. All three station were located from inside
                    the crescent on the east side Station 1 was in the middle. Station 2 was on
                    the north end. Station 3 was at the south end. Oysters were collected by
                    hand and tongs.



                                                      473











              Year IV - Year IV stations were collected in the same locations as Year 111.
                    Oysters were very small in large clumps and the shells were very thin.

              Year  V - Oyster stations were in the same location as previously collected.
                    Oysters were much less numerous this year.

              Year  VI - Collection sites remained the same as those sampled last year
                    (29010.25'N, 83003.00'W), but oysters were smaller and less numerous.

              Year VII - Due to extremely low tidal conditions the boat could not be launched
                    this year; therefore, the site was moved approximately 1 km to the north
                    (29'12.28 north 83"04.34 west), adjacent to the boat launch area. There are
                    numerous reefs in the vicinity of the launch area. All three stations were
                    located on the south side of the launch area. Station 1 was 50 m south of the
                    launch, Station 2 was 50 m farther south , and Station 3 was another 50 m
                    to the south.

              Year VIII, & IX - Oysters were collected at the same stations as described for
                    Year VIL

                 )DIENTCOLLEMONS

              Year I - Sediments were collocated with each of the oyster stations.

              Year II - Sediments were taken from isolated pockets of sandy, shelly mud on the
                    inside curve of the island and were adjacent to each oyster station. Many
                    shell fragments were present over the entire bottom. A box core and
                    wading was required to locate and retrieve the sediment samples.

              Year III - Sediments in Year III were located within 5 m of the oyster stations
                    and were sandy mud with some shells.

              Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                    years.

              SAMPLINGMETHOD

                    Oysters - tongs and by hand
                    Sediment - box core

              WATER DEPTH - intertidal, 0.2 - 0.4 meters

              POSSEBLE CONTANONANTS - The site was mainly open water and no sources of
              contamination were observed. The site was miles from any habitation, industry
              or port facility of any size. Effluents from Suwannee River may have influenced
              the site.






                                                      474










                 ENVIRONhUM14TAL DATA

                                              Salinity           Temperature           Dates Sampled
                                                (700)                   (OC)

                      YEAR 1                    14.3                    23.0           27 February 1986
                      YEAR 11                   23.0                    20.0           28 February 1987
                      YEAR 111                  31.0                    14.0           19 February 1988
                      YEAR IV                   26.0                    18.0           28 January 1989
                      YEAR V                    25.0                    18.0           29 January 1990
                      YEAR VI                   23.0                    16.0           29 January 1991
                      YEAR VII                  24.0                    17.0           30 January 1992
                      YEAR VIII                 27.0                    22.0           13 January 1993
                      YEAR IEK                  19.0                    4.0            19 January 1994



































                                                                 475















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                                                           Site #43 (CKBP), Cedar Key Black Point.
    IeDeLmme Mapping Company. Map Taken from Florida Atlas and Gazetteer. Reproduced with permission.













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              SrIE NUMBER - 70                               DESIGNATOR - TBOT

              SITE - OLD TAMPA BAY, TAMPA BAY, FL

              NON[INAL SITE CENTER - 28001.48'N              82037.95W

              LOCATED ON NOS CHART            11413

              SITE ACCESS - To reach the site, take Highway 275 to 92 west and then take
              Hillsborough Avenue toward Tampa Bay Downs. Turn south on Double Branch
              Road and proceed to State Street, turn left and go to the boat ramp at the end of the
              street.

              SITE DESCRIPTION - This site, established in Year III, produced the most
              abundant oysters collected in the Tampa Bay area. Substrate was primarily
              sandy mud with pockets of oozy mud scattered around the shoreline.

              OYSTERCOLLECTIONS

              Year III - Oysters at all three stations were well developed and sorted. They were
                    of moderate size and looked healthy. They were plentiful, were easily
                    picked by hand and required little sorting. All three reefs were clearly
                    visible and partially exposed at low tide, but were still easily accessible at
                    high tide by wading. Oyster Station 1 was located - 50 m north of the boat
                    ramp. The reef extended across water from east to west and was exposed at
                    low tide. Station 2 was a small subtidal reef - 10 m east of the end of the
                    boat ramp. Station 3 was 50-m southeast of the boat ramp where the creek
                    forks on the east side of the channel.

              Year  IV - The same three stations were sampled in Year IV as were first
                    established in Year III. The site remained closed to harvesting, and the
                    oysters from external appearance and abundance appeared in good shape.

              Years V & VI - The same stations were sampled again these year. Oysters were
                    mostly alive and were in better condition than those at sampling sites to the
                    north.

              Year VII - This site was not sampled this year.

              Years VIII & IX - Oysters were collected at the same stations as described for
                    Year III.


              SEDDMENT COLLECTIONS

              Year III - Sediments were collocated with the oysters. Sediments were collected
                    with the teflon scoop directly from the bottom substrate.

              Year IV - No sediments were sampled.



                                                       481










               Year V - Sediment samples were taken adjacent to each of the oyster collection
                      stations. Sediment was a thin silt layer over hard sand found along the
                      edge of the mangroves. Station 1 was in a small cove on the west end of the
                      reef. Station 2 was 20 m northwest of the oyster site along the shore.
                      Station 3 was 20 m to the east in the small cove on the north side of the
                      channel.

               Year VI - Sediments (NIST) were collected in the same locations described in
                      Year V.

               Years VII, VIII, & IX - No sediments were sampled during these years.

               SAMPLING METHOD

                      Oysters - hand
                      Sediment - hand

               WATER DEPTH - intertidal, 0 - 0.3 meters

               POSSIBLE CONTAMINANTS - Contamination at the site was not obvious, but
               mobile home and housing developments were present upstream from the
               collection site. No industrial contaminant sources were observed.


               ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                  (OC)

                    YEAR 111               10.0                  14.0          22 February 1988
                    YEAR IV                  -                   20.0          29 January 1989
                    YEAR V                 16.7                  22.0          30 January 1990
                    YEAR VI                  7.3                 16.7          30 January 1991
                    YEAR VIII              14.0                  22.0          14 January 1993
                    YEAR IX                  2.0                 13.0          20 January 1994

















                                                           4&










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  )eLmme Mapping Company. Map Taken ftont Florida Atlas and Gazetteer. Peproduced with permission.










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             SrIE NUMBER 44                               DESIGNATOR - TBPB

             SITE - PAPYS BAYOU, TAMPA BAY, FL

             NOM[INAL SYIE CENTER - 27050.53'N            82036.62W


             LOCATED ON NOS CHART # - 11413

             SITE ACCESS - The site is accessed by boat or by driving to the site. By boat,
             depart the city marina in St. Petersburg and cross the west side of Tampa Bay to
             Papys Bayou (-30 minutes). Access to the site for sampling by wading is
             accomplished by driving to the Weeden Island Wildlife Refuge via Weeden Drive.
             In Year VII a boat was launched at Gandy Bridge Marina. The site was reached
             by traveling out the channel parallel to the highway to Tampa Bay and then south
             to channel marker 15, then turn west to the entrance to Papys Bayou. This ride
             takes appro-3dmately 20 minutes.

             SITE DESCRIPTION - The oyster site in Year I was a small intertidal reef (found
             by following channel markers to where reef almost blocked channel) at the back
             end of the bayou and the mangrove roots along the adjacent shoreline. A wood
             and steel pier-type of structure was at the end of the bayou where samples were
             taken. In Year II, the station was moved to within 100 m of the old bridge at the
             end of Weeden Drive.


             OYSTERCOLLECTIONS,

             Year I - Oysters were collected on a submerged oyster reef surrounding an old
                   wooden and metal structure on the east side of the bayou in a mangrove
                   area. Landmarks to locate the site were 1400 from the tall radio tower and
                   195' from the smoke stacks at the power plant. The sites were separated by
                   100 m.

             Year  II - Year II collections were made in the same location. Oyster stations
                   were not differentiated geographically this year.

             Year III - In Year III, we drove to the site and collected the oysters by hand. A
                   large healthy reef was located 100 m from the wooden pier at the end of
                   Weeden Pond. Oysters were numerous but distinct stations were not
                   recognized.

             Years IV, V, & VI - The site was in the same position as sampled in Year III. No
                   station distinctions were made. The stations were on the first large reef
                   south of the pier at the end of Weeden Road. The site was best accessed by
                   wading from the pier. The Year III and IV site was approidmately 100-200
                   m north of the site sampled in Year II. Oysters were very abundant and
                   accessible. In Year VI the oysters were very small and many had died
                   recently.





                                                    487









              Year VII - The same location was sampled again this year. Oysters were
                     numerous but were very small.
              Year VIII - Oysters were not abundant on the reef, so the @ite was moved 30 m to
                     the fishing pier.

              Year IX - Station 1 oysters were collected from the mangroves at the shore end of
                     the pier, Station 3 oysters from the distal end of the pier, and Station 2
                     oysters from the midpoint of the pier between Stations 1 & 3.

                 'T IDMENT COLLECTIONS

              Years I & II - Sediments from the oyster site were all sand and shell. Sediments
                     were collected on Weeden Island north of Harbor Isle. Sediments were
                     intertidal sands with some humic material.

              Year III - Sediments were collected along the mangrove-lined shoreline. Station
                     I was at the small boat ramp. Station 2 was 50 m to the north around the
                     point. Station 3 was another 30 rn north in a small cove. The majority of
                     the bottom was sand except along the mangrove tree margin (1-2 m zone)
                     where fine-grained silt was found.

              Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                     years.

              SAWLWG METHOD

                     Oysters - hand
                     Sediment - box core

              WATER DEPTH - intertidal, 0 - 0.5 meters
              POSSIBLE CONTAMINANTS - Sources of contamination included heavy
              recreational boating in the restricted embayment, and an electric power
              generation plant near the bay.

              ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (9010)               (0c)

                   YEAR 1                  28.0                 27.0           23 February 1986
                   YEAR 11                 21.0                 24.0           28 February 1987
                   YEAR 111                30.0                 17.0           21 February 1988
                   YEAR IV                 21.0                 23.0           29 January 1989
                   YEAR V                  25.0                 23.0           30 January 1990
                   YEAR VI                 30.0                 19.0           31 January 1991
                   YEAR VII                28.0                 18.0           28 January 1992
                   YEAR VIII               15.0                 21.0           15 January 1993
                   YEAR IX                 28.0                 14.0           20 January 1994


                                                          488









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                                                         Site #44 (TBPB), Tampa Bay Papys Bayou.
  OeLorlne Mapping Company. Map Taken from Florida Adas and Gazetteer. Reproduced with permission-
















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              SITE N1JJNMM 45                                DESIGNATOR - TBHB

              SITE - HILLSBOROUGH BAY, TAMPA BAY, FL

              NO51INAL SITE CENTER - 27051.28N               82023.75V

              LOCATED ON NOS CE[ART # - 11413

              SITE ACCESS - The boat was launched at the ramp on the northwest corner of the
              Highway 41 bridge over the Alafia River.

              SITE DESCRIPTION - This site was located on the north bank of the Alafia River.
              The sites were established along the riprap shoreline on the east side of channel
              marker 15.


              OYSTERCOLLECTIONS

              Years I & II - The only oysters in the area were attached to rock rubble and tree
                    roots at the shell hash beach on the north bank of the river next to the
                    phosphate plant (downstream from their dock). The bank was steep and
                    quickly became too deep for sampling. Oysters were very sparse, and this
                    site may not provide sufficient oysters for extended study. No geographic
                    distinction could be made in the oyster collection sites. The three stations
                    were contiguous to each other. Station 1 was on the west end and Station 3
                    was on the east end of the site.

              Year III - In Year III, there were only enough oysters for one station. This site
                    should be dropped because of the lack of oysters. There were not likely to be
                    other sources for oysters in the Hillsborough area, since this was the best
                    we could locate in Year I.

              Years 1V, V, VI, & VII - The site was not sampled during these years.

              Year VIII - Oysters were collected at the same stations as described in Year III.
                    Oysters were numerous but small.

              Year IX - The only oysters to be found this year were directly across the river on
                    the south side, attached to mangrove roots. The three stations were
                    contiguous to one another and were not distinguished.

                  WIENTCOLLECTIONS

              Year I - Sediments of fine texture in close proximity to and representative of the
                    site were not possible. Therefore, a site was selected approximately 0.5
                    miles east. Station 1 was between the boat launch and oyster site. Station 2
                    was from the boat basin across the river from the boat launch. Station 3
                    from upstream past the railroad bridge to the south side of the river in front
                    of Captain D's Marina.



                                                       493









              Years H & III - Sediments for Years II and III were at the same sites, except
                     that no sediments were located for Station 1.

              Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                     years.

              SAMPLINGMETHOD

                     Oysters - hand
                     Sediment - box core

              WATER DEPTII - subtidal, 0.6 meters

              POSSIBLE CONTAMINANTS - Obvious sources of contamination included the
              phosphate plant which was directly adjacent to the oyster collection site. In the
              case of two sediment samples, the boat basin/marinas were sources of
              contamination.


              ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           NCO                  (0c)

                   YEAR 1                  22.0                 22.0          24 February 1986
                   YEARII                  21.0                 20.0            1 March 1987
                   YEAR III                  -                  23.0          20 February 1988
                   YEAR VIII               15.0                 20.0           15 January 1993
                   YEAR IX                 15.0                 15.0           21 January 1994
























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                                                                                            Site #45 (TBHB), Tampa Bay Hillsborough Bay.

   leLorme Mapping Company. Map Taken from Florida Ades ad Gazenea. Peproduced with permission.










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             SITE NUMBER 46                                DESIGNATOR - TBCB

             SITE - COCKROACH BAY, TAMPA BAY, FL

             NOM[INAL SITE CENTER - 27040.55'N             82030.56'W


             LOCATED ON NOS CHART # - 11414

             SITE ACCESS - The site is accessed by driving to Highway 41 and taking the
             Cockroach Bay Road to the west. The launch ramp is at the end of the road.
             Shallow draft boats with tilt motors are required. Access to the site requires
             winding around many small islands to the open bay to the east. Upon entering
             the open bay, proceed east to a very small island (mostly subtidal) near the
             southeast shore.

             SITE DESCRIPTION - The site was located on an exposed reef on the south side of
             Cockroach Bay. Oysters were collected by hand from the subtidal portions of the
             reef. Neither the sediments or oyster collection stations could be differentiated
             geographically around the small island. All were composites of random samples
             from around the island.


             OYSTER COLLECTIONS

             Year I - Oysters were collected from an exposed reef on the south side of the bay.
                    Station 1 was on the reef which is 195' from the tall radio tower. Station 2
                    was along mangrove roots -100 m east of Station 1. Station 3 was -100 m
                    east of Station 1 and 30 m north of Station 2.

             Years II, III, IV, V, VI, VII, & VIII - In these years, all stations were taken
                    from the same very small reef in the back (south side) of Cockroach Bay. No
                    station distinctions could be made due to the small area from which oysters
                    were available. However, Cockroach Bay has many other sites suitable for
                    oyster collection, both from the roots of mangroves and other reefs nearer
                    the mouth of the bay.

             Year IX - The site was not scheduled to be sampled this year.

             SEDINM'qT COLLECTIONS

             Years I, II, & III - Sediments were found in isolated pockets around this reef and
                    were limited to the extreme upper layer.

             Years 1-V & V - No sediments were sampled during these years.

             Year VI - Sediments (NIST) were collected from around the small island       where
             the oysters were collected.

             Years VII, VIII, & IX - No sediments were sampled during these years.




                                                     499











               SAMPLINGMETHOD

                      Oysters - hand
                      Sediment - Sediments were sampled by hand with the teflon scoop, and
                             required compositing samples from many scoops in different pockets
                             to acquire sufficient material.

               WATER DEPTH - intertidal, 0 to 0.6 meters

               POSSIBLE CONTAMINANTS - There were no obvious sources of contamination
               in this very secluded site, although the surrounding area appeared to be vegetable
               and fruit farms.


               ENVIRONMENTAL DATA

                                          Salinity          Temperature         Dates Sampled
                                            (%0)                 (0c)

                    YEAR     1              28.0                 25.0           24 February 1986
                    YEAR     11             24.0                 20.0            1 March 1987
                    YEAR     111            30.0                 24.0           20 February 1988
                    YEAR     IV             22.0                 23.0           31 January 1989
                    YEAR V                  26.0                 22.0           1 February 1990
                    YEAR     VI             30.0                 27.0           30 January 1991
                    YEAR     VII            29.3                 20.0           29 January 1992
                    YEAR     VIII           14.0                 22.0           15 January 1993


























                                                           500






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   @DeLorrne Mapping Company. Map Taken from Florida Atlas and Gazetteer. Reproducedwith permission.









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             SITE NUMBER 47                                 DESIGNATOR - TBMK

             SITE - MULLET KEY BAYOU, TAMPA BAY, FL

             NOMINAL SHE CENTER - 27037.28'N                82043.62W


             LOCATED ON NOS CHART # - 11414

             SITE ACCESS - The site is accessed by automobile from Fort DeSoto County Park
             on Mullet Key. To reach the site, travel out Highway 679 turn right on Anderson
             Blvd. Turn right (north) on the a small shell road north of the pier parking lot.
             Travel until the road ends at the water.

             SITE DESCRIPTION - The site was located in the southwest corner of Mullet Key
             Bayou north of Family Fishing Pier 1.

             OYSIERCOLLECTIONS

             Year I - Oysters were collected at the edge of shore in intertidal waters on the
                    sand bottom and on mangrove roots at three locations roughly 200 m apart
                    Station 1 was 50 m east of Fort Road just offshore from mangrove roots.
                    Station 2 was 50 m to the right of Fort Road. Station 3 was at the end of the
                    Fort Road and west of Station 2. Oysters were small and not plentiful.

             Years II, III, IV, & V - Station 1 was located 30 m from the ramp, just south of
                    the three poles and by the mangroves. Station 2 was located at and to the
                    east of the ramp, approximately 150 m east of Station 1. Station 3 was 100 m
                    east of Station 2, at the second point of mangroves where there is a small
                    reef.

             Years  VI, VII, VIII, & IX - The same stations described for Years II-V were
                    sampled again these years.

             SEDIMENT COLLECTIONS

             Years I, II, & III - Sediment samples were taken 10 m away from each of the
                    oyster station.

             Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                    years.

             SAMPLING METHOD

                    Oysters - hand
                    Sediment - box core

             WATER DEPTH - intertidal, 0 to 0.5 meters

             POSSIBLE CONTAMINANTS - There were no observable contamination sources.



                                                      505











              ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           ( t7oo )             (OC)

                   YEAR     1              34.0                 24.0           25 February 1986
                   YEAR     11             20.0                 19.0            1 March 1987
                   YEAR     111            37.0                 29.0           19 February 1988
                   YEAR     IV             32.0                 21.0           31 January 1989
                   YEAR V                  31.0                 23.0           31 January 1990
                   YEAR VI                 35.0                 22.0           30 January 1991
                   YEAR VII                35.0                 16.0           28 January 1992
                   YEAR     VIII           33.0                 22.0           14 January 1993
                   YEARIX                  33.0                 15.0           21 January 1994


















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                                                                                      Site #47 (TBMK), Tampa Bay Mullet Key Bayou (from chart 11411).

















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              SITE NUMBER 77                                   DESIGNATOR - TBKA

              SITE - KNIGHT AIRPORT, TAMPA BAY, FL

              NOM091AL SITE CENTER - 27054.46N                 82027.29V

              LOCATED ON NOS CHART              11413

              SITE ACCESS - The stations were established on a jetty which extends from the
              end of the runway into the bay. The site is located on the south end of the Davis
              Islands, adjacent to the Peter 0. Knight airport and the Davis Islands Yacht
              Club. To reach this site from St. Petersburg, take 1-275 east and take exit 24, go
              south on Armenia to Swann Ave., then east to join Bayshore Blvd. Cross the
              bridge to the Davis Islands. Stay on west Davis Blvd. and then exit right on
              Airport to Marinique which follows the seawall.

              SITE DESCRIPTION - The site was not established and sampled until Year IV.
              Oysters were intertidal and were attached to the rocks of the jetty. The sampling
              site was located along the seawall and jetties.

              OYSTER COLLECTIONS

              Years TV, V, VI, VII, VIII, & IX - Station 1 was located along the seawall
                     beginning at the junction of the seawall and jetty on the east side of the jetty.
                     Station 2 was at the bay end of the jetty, and Station 3 was on the west side of
                     the jetty beginning at the seawall.

              SEDIM[ENT COLLECTIONS

              Years IV & V - Suitable fine grained sediments were difficult to obtain near the
                     site because the bottom was mostly sand. Isolated pockets of fine black
                     muds with some sand were located along the seawall some distance from
                     the oysters sites. Station 1 was about midway down the seawall to the the
                     east, and was sampled by boxcore from the seawall. Station 3 was about
                     200 m to the west of the seawall/jetty junction, at the first joint in the
                     concrete wall (from the west). Station 2 sediments were from the far
                     eastern end of the seawall, at the corner closest to the airport terminal
                     building.

              Year VI, VII, VIII, & IX - No sediments were sampled during these years.

              SAMPLINGM[ETHOD

                     Oysters - hand
                     Sediments - box core

              WATER DEPTH - intertidal, 0.1 meters

              POSSIBLE CONTAMINANTS - No sources of contaminants were identified.



                                                         511










               ENVIRONMENTAL DATA

                                         Salinity          Temperature          Dates Sampled
                                            (%o)                 (0c)

                    YEAR IV                 20.7                 24.0           31 January 1989
                    YEAR V                  24.0                 22.0           31 January 1990
                    YEAR VI                 27.0                 22.0           29 January 1991
                    YEAR VII                28.0                 18.0           29 January 1992
                    YEAR VIII               27.0                 22.0           14 January 1993
                    YEAR IX                 30.0                 14.0           20 January 1994







































                                                           512














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                                                                           Site #77 (TBKA), Tampa Bay K. Airport.

  'IT)el nrme Mapping Company. Map Taken from Florida Atlas and Gazetteer. Peproduced with permission.















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              SrIE NUMBER 76                                 DESIGNATOR - TBNP

              SITE - NAVAREZ PARK, TAMPA BAY, FL

              NONIINAL SITE CENTER - 27047.28'N              82c45.28V

              LOCATED ON NOS CELART # - 11413

              SITE ACCESS - The site, which was established in Year IV, is located at the boat
              ramp in Navarez Park which is located on the east side of Boca Ciega Bay north of
              the Treasure Island Causeway. To reach the site, travel west on 5th Ave. north
              and turn north on Park Street. Turn off of Park street to the west onto Elbow
              Lane.

              SITE DESCRJ[PTION - This site has an old boat basin and sand ramp that was no
              longer used and two concrete ramps and a fishing pier. All three stations were
              located south of the new boat ramp.

              OYSTER COLLECTIONS,

              Years IV, V, & VI - Oysters were abundant at all three stations. Station 1 was
                     along the. north and east side of the boat basin. Oysters were attached to the
                     concrete bulkhead, on rocks and debris on the bottom, and on the sandy
                     bottom. Station 2 was 50 m to the southwest across the boat basin on a point
                     that jutted to the north. The site began at the northernmost point of land
                     and continued south for approximately 50 m. Oysters were attached to
                     concrete rubble and on the sandy bottom. Station 3 was 100 m south of
                     Station 2 on a small point of land that jutted out to the west. Oysters were
                     attached to the concrete rubble.

              Years VII & VIII - The same stations were sampled again these years.

                 I)IMENT COLLECTIONS


              Year rV - All three sediment stations were located within the small boat basin.
                     Sediments outside of the basin were very sandy. Station 1 was on the north
                     side of the basin along the concrete bulkhead. Station 2 was at the east end
                     of the basin. Station 3 was on the south side of the basin just inside the
                     mouth and directly west of the pole with the tidal gauge. (This station was
                     collocated with oyster Station 2). Sediments were fine grained soft black-
                     gray silty sand.

              Year V - Sediments were not sampled this year.

              Year VI - Sediments (NIST) were collected at the same locations described for
                     Year IV.

              Years VII, VIII, & IX - Sediments were not sampled during these years.



                                                       517











              SAMPLINGMETHOD

                      Oysters - hand
                      Sediments - box core

              WATER DEPTH - intertidal, 0 to 0.3 meters

              POSSIBLE CONTAMINANTS - Several sources for contamination were possible
              such as urban runoff and pollution from marine boat traffic.

              ENVIRONMENTAL DATA

                                         Salinity           Temperature         Dates Sampled
                                            (700)                (0c)


                    YEAR IV                 31.3                 24.0           31 January 1989
                    YEAR V                  31.0                 20.0           1 February 1990
                    YEAR VI                 34.0                 19.5           31 January 1991
                    YEAR VII                33.0                 17.0           28 January 1992
                    YEAR VIII               'U. 0                22.0           14 January 1993





























                                                           518











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                                                                                          Site #76 (TBNP), Tampa BaY Navarez Park.

   QDeLorme Mapping Company.                   M81) Taken from Florida Atlas and Gazetteer. Reproduced -with permission.









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                                                                                     Site #76 (TBNP), Tampa Bay Navarez Park (from chart 11411).


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              SITE NUMBER 48                                DESIGNATOR - CBBI

              SITE - BIRD ISLAND, CHARLOTTE HARBOR, FL

              NOMP4AL SITE CENTER - 26030.73N               82002.18V


              LOCATED ON NOS CHART # - 11427

              SITE ACCESS - Boat launch is at the marinalbait shop next to St. James
              Restaurant on the main road which runs the length of Pine Island. Proceed
              down the canal, turn left and enter the bay. Join the channel markers and
              proceed to Bird Island After the second year, the launch site was moved to the
              public ramp at Red Fish Point in Ft. Meyers. To reach the site proceed down the
              Calooshatchee River along the ICWW past Big Shell Island. Turn north at
              channel marker 2 and 2A. This will take you to the west of Bird Island. An
              alternate launch site is at the Punta Rassa Ramp.

              SITE DESCRIPTION - The site was located in San Carlos Bay in the Matlacha
              Pass National Wildlife Refuge. The site was on a reef which was directly north of
              Bird Island and northeast of Merwin Key. Oysters were plentiful on the subtidal
              portions of the reef and could be collected either by hand or with tongs.

              OYSTER COLLECUONS

              Year I - Collections were made on the submerged reef approximately 150 m from
                    Bird Island. Bearings to locate Station 1 were 305' from Bird Island, 325'
                    from the end of large bridge, and 700 from the north end of Merwin Key.
                    Station 2 was 250 m north of Bird Island at 210' and 325' from the large
                    bridge, and 600 from the north end of Merwin Key. Station 3 was about 200
                    m from Bird Island at 290', 3250 from the bridge, and 700 from the north
                    end of Merwin Key.

              Year II - Year II stations were in approximately the same locations. Station 1
                    was on the east side of Bird Island. Station 2 was north of Bird Island, and
                    Station 3 was on the west side of Bird Island.

              Years III & IV - Years III and IV oyster stations were collected on the large reef
                    north of Bird Island. Bearing to Bird Island were 110' and 1300 to the high
                    rise condo.

              Years V & VI - Oysters were collected approximately 500 m northwest of Bird
                    Island at the second reef. This was the same general location as last year.
                    Station 1 was on the east end of the reef, Station 2 was in the middle 100 m
                    west of #1, and Station 3 was on the west end of the reef 150 m from the last
                    station.

              Years VII, V111, & IX - The same stations described for Year V were sampled
                    again these years.



                                                      523











                   )IMENT COLLECTIONS

              Year I - Sediments were collected adjacent to the oyster stations. No fine
                     sediments were found anywhere near the oyster site.

              Year II - Sediments were sampled west of Bird Island -50-100 m from the oyster
                     Station 3 (Station 1) and then again 200 m farther west (Station 2). These
                     sediments were silty sand at best and contained significant amounts of sea
                     grasses. The third sediment was collected at the mouth of the dredged
                     channel which led to the boat launch. This site was several miles from
                     Bird Island and about 0.5-1 mile from the boat launch.

              Year III - Sediments were collected approximately 30 m south of each oyster
                     station.

              Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                     years.

              SAMPLINGMETHOD

                     Oysters - tongs and by hand
                     Sediment - box core

              WATER DEPTH - intertidal, 0 to 0.8 meters

              POSSIBLE CONTAMINANTS - There were no obvious sources of contamination
              at the Bird Island site. The site was in the open in a large bay. Station 3
              sediments would not be considered representative of contaminant exposure at the
              oyster site. However, it was the only source of sediments which clearly met the
              size criteria, and the sediments would have been subject to contamination from
              residential and recreational boating areas which surrounded the canal.

              ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                 (0c)

                    YEAR 1                 34.0                 27.0           21 February 1986
                    YEAR 11                31.0                 20.0            2 March 1987
                    YEAR 111               31.0                 20.0           22 February 1988
                    YEAR IV                33.0                 22.0           30 January 1989
                    YEAR V                 30.3                 25.0           31 January 1990
                    YEAR VI                30.0                 26.0           31 January 1991
                    YEAR VII               33.0                 19.0           28 January 1992
                    YEAR VIII              24.0                 20.0           18 January 1993
                    YEAR IX                26.0                 16.0           21 January 1994






                                                           524





















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                                                                                                                                                                                               hydrography north of Sanibel Island Into
                                                                                                                                                                                               k4allacha Pass Is from surveys made prior
                                      P I N E                                                                    (.2                                                                           to 1900.
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                                                                                                Site #48 (CBBI), Charlotte Harbor Bird Island (from chart 11427).








































































































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              SITE NUMBER 71                                   DESIGNATOR - CBFM

              SITE - PORT MEYERS, CHARLOTTE HARBOR, FL

              NOMINAL SITE CENTER - 26033.50'N                 81055.37V

                          ON NOS CE[ART         11427

              SITE ACCESS - This site, established in' Year III, is reached by boat launched at
              the public boat ramp at Redfish Point. Proceed up the Calooshatchee River to the
              Cape Coral Bridge.. The site is on.the east end of the bridge.

              SITE DESCRIPTION - -The site was located on the east end of the bridge on both
              sides of the highway. Small oyster clumps       along with clams and barnacles were
              attached along the base of the bridge and were in clumps on the sand and shell
              bottom.


              OYSTER COLLECTIONS

              Year III - Oyster Station I was on the east end of the bridge on' the northeast side.
                     Oyster'Station 2 was on the south side of the Cape Coral,Bridge. Station 3
                     was 100 m to the south along the bulkhead.

              Years IV, V, Vil Vii, & VIII - Oyster collections stations     'were the same sites as
                     sampled in Year III.
              Year IX - The site was not scheduled to be sampled this year.-

                  ODMENTC011ECTIONS;

              .Year III - Sediments were, collected 50 to 100 m offshore from the oyster stations.
                     Sediments were primarily sand. with some mud.. Sediment Station 1 was
                     collected about 50 m north of the oyster collection site in I to 1.5 ni of water.
                     Sediment. Station 2 was located 75 m south-. of the oyster collection site.
                     Sediment Station 3 was in i.5'm of water -100 m to the northwest of the
                     oyster collection area.

              Year TV - Sediments were not sampled this year.

              Year   V - Sediment     samples were    collected adjacent    to each   of the oysters
                     collection sites. Suitable sediment occurred as a thin layer of silt in small
                     depressions. Most of, the Tecent sediment appeared        to be associated with
                     recent road construction.

              Year VI - Sediments were not collected this year.

              Years VII, VIII, & IX     No sediments were collected during these years.




                                                        529











               SAMPLINGMETHODS

                      Oysters - hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0.1 - 0.6 m

               POSSIBLE. CONTAMINANTS - Contamination was present from highway
               drainage and possibly from septic systems.

               ENVIRONMEN'r.4L DATA

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                 (OC)

                    YEAR 111               24.0                                22 February 1988
                    YEAR IV                20.3                 20.0           30 January 1989
                    YEAR V                 24.0                 23.0           31 January 1990
                    YEAR VI                19.0                 25.0           31 January 1991
                    YEAR VII               26.0                 18.0           28 January 1992
                    YEAR VIII              14.0                 20.0           18 January 1993

















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                                              Site #71 (CBFM), Charlotte Harbor Fort Meyers (from chart 11427).






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              SrIE NUMBER 49                                DESIG   NATOR - NBNB

              SITE - NAPLES BAY, NAPLES BAY, FL.
              NOMINAL SITE CENTER - 26066.78'N               81-47..15'W

              LOCATED ON NOS CHART            11430,11429

              SITE ACCESS - The boat is launched from Bayview Park on the south side of
              Naples. It is accessible almost directly from the interstate via the exit for Marco
              Island. Proceed south on,Highway 951 to Rattlesnake Hammock Road (Highway
              864). Go across Highway 41 to Thommason Road and proceed to the end of the
              road at Bayview Park. Proceed by boat north. from the park up the channel to
              channel marker 24.

              SITE DESCRIPTION - The oyster re        Iefs were located to the east and adjacent to
              channel marker 24. The old reefs were primarily consolidated and cemented old
              shell fragments.

              OYSTER COLLECTIONS

              Year  I - Year I oysters were collected   by hand.   Station 1  was at a reef in a
                     residential area with,bearing of 185' to channel marker'24; 268' to channel
                     marker, 26. Station 1 was at the first reef adjacent to channel marker "Red
                     24". Station 2 was at.the second reef with bearing of 112' to channel marker
                     22; 298' to channel marker 24. Station 3 was 100 m east of Station 2 at the
                     s
                       all reef with a tree.
                     m

              Year II - The, oysters during Year II were collected with tongs from the subtidal
                     portions of the reef. . Stations were not differentiated, but rather were
                     composites of random ly collected oysters   around the small, mostly     dead
                     reef.

              Year III - Year III oysters were collected in the same area but, the stations were
                     not distinguished.

              Years  IV, V, VI, V11, & VIII - The site was the same as sampled previously, a
                     submerged reef adjacent to channel marker 24. Station I was the
                     submerged reef with the single mangrove tree growing on it. Station 2 was
                     on the east side of the'reef; Station 3 was sampled from clumps of oysters by
                     the point to the east of the Station 2.

              Year IX - The site was not scheduled to be sampled this year.

                  )DOMW COLLECTIONS

              Year I - Sediments from the area were almost non-existent, due to the nature of
                     the consolidated shell bottom. Fine sediments were located only in very
                     small isolatedpockets that were found bychance and in boating channels



                                                      535









                      that were dredged to the homesites. Year I sediments were collected along
                      the shore east of the respective oyster stations.

               Year II - Sediment Station 1 was among the oyster stations. Sediment Station 2
                      was north of the oyster site in to the channel east and north of channel
                      marker 26 & 27 (channel identified as Tarpon Road and Kingfisher Road).
                      Sediment #3 was from the entrance to the boat basin at the launch ramp in
                      Bayland Park.

               .Years III, IV, & V - Sediments were located in the same areas as sampled in
                      Year II.

               Years VI, VII, VIII, & IX - No sediments were sampled during these years.

               SAMIPLING ME`I1IOD

                      Oysters'- tongs and by hand
                      Sediment - box core

               WATER DEPTH - intertidal, 0.2 to 1 meter

               POSSIBLE CONTAMINANTS - Sources of contamination would be from very.
               heavy recreational boating and'dense residential development along and farther
               upstream on the waterfront.

               ENVIRONMENTAL DATA

                                         Salinity          Temperature         Dates Sampled
                                            (0/00)               (0c)

                    YEAR 1                  27.6                 23.0          21 February 1986
                    YEAR 11                 25.0                 19.0           2 March 1987
                    YEAR 111                23.0                 20.0          20 February 1988
                    YEAR IV                  -                   23.0          29 January 1989
                    YEAR V                  34.0                 22.0          29 January 1990
                    YEAR VI                 32.0                 25.0          1 February 1991
                    YEAR VII                36.0                 19.0          27 January 1992
                    YEAR VIII               30.0                 23.0          18 January 1993














                                                           536

















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                                                                                             Site #49 (NBNB), Naples Bay Naples

   DeL,orme Mapping Company. Map Taken from Florida Atlas and Gazencer. Reproduced with permission.








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               SITE NUMBER'60                                   DESIGNATOR - RBHC

               SITE - HENDERSON CREEK, ROOKERY BAY, FL

               NOMINAL SITE CENTER - 26001.50'N                 81044.20V

               LOCATED ON NOS CHART              11430,11429

               SITE ACCESS'-- The site was accessed from 1-75 by driving west.(or south) on
               Florida 951, @- 2.6 miles past the intersection of U.S. Highway 41, Turn right on
               Shell Island Road. Shell Island Road is marked by a sign        for the Briggs Nature
               Conserancy and Marine Research Lab. Proceed to the Rookery Monument, past
               the Rookery Headquarters and to the boat ramp at the end of the road.

               SITE DESCRIPTION - The site was located in Rookery Bay Aquatic Preserve in
               Henderson Creek. . AR,three sites were near the mouth of the creek, in the vicinity
               of the Childrens, Monument,

               OYSTER COLLECT, IONS

               Year. I  The oyster population At 'this site was     not large but was    sufficient for
                     repeated. sampling.     Oysters were collected by1and from a        subtidal reef
                     along the.shbre and from mangrove roots. Station I was a submerged bed
                     adjacent to mangrove clump, where mangroves constrict Henderson Creek
                     at Rookery Bay.- Station I was to the south. Station 2 was -250 m southeast
                     of, Station, 110" cated. at 82' from the monument. Station 3 was -10 m west of
                     monument...

               Years II, III, IV, V, VI, VII, &  VIII - In these years the site was located in the
                     same area around the       monument along the shore line. Oysters were
                     located on clumps on the mangrove roots. Station 1 was south of the
                     monument; Station 2 was north of the monument; and Station 3 was
                     approximately. 1.00 to-the northeast.
               Year IX - The site was'the same as previously designated, at the monument.
                     Station@ I was on the same shoreline as the monument, approx. 150 m to the
                     south at the first bare reef patch in the mangroves. Station 2 was located on
                     the shore opposite the monument, at a small cove in the mangroves, next to
                     the manatee sign. Station 3 was located on the shoreline opposite the
                     monument, approx. .200 m to the northeast at the bare patch in the
                     mangroves, where the channel begins to constrict from the passage out of
                     'Henderson Reef into Rookery Bay.

               SEDMIENT COLLECTIONS

               Years 4 11, & III  Sediments were taken from among the mangrove roots at each
                     station.






                                                         541









              Years IV, V, VI, VII, VIII, & IX - Sediments were not sampled during these
                     years.

              SAMPLING METHOD

                     Oysters - hand
                     Sediment - hand.

              WATER DEPM - intertidal, 0.2 to 0.5 meters

              POSSEBLE CONTAAHNANTS - No observable contamination factors were noted.

              ENVIRONMENTAL DAT

                                         Salinity          Temperature         Dates Sampled
                                           (%0)                 (OC)

                   YEAR. 1                 34.0                 23.0           20 February 1986
                   YEAR 11                 16.6                 220             2 March 1987
                   YEAR 111                16.0                 21*0           21 February 1988
                   YEAR IV                                      23.0           30 January 1989
                   YEAR V                  35.0                 24.0           30 January 1990
                   YEAR VI                 34.0                 25.0           2 February 1991
                   YEAR VII                37.0                 20.0           27 January 1992
                   YEAR VIII               35.0                 210            18 January 1993
                   YEAR IX                 34.0                 18*0           21 January 1994

















                                                           154
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                                                                        Site #60 (RBHC), Rookery Bay Henderson Creek.

    Lorme Mapping Company. Map Taken ftom Florida Atlas and Gazetteer. Reproduced with permission.












                                                                                                            "of the District Engineer, Corps of Engineers
                           TION                                                                             /           in Jacksonville, Florida.
                           d stay clear of laMe                                                                         Anchorage regulations may be obtained at
                           ornment vessiels ovm                          CL a                                           ft Office of the Commander, 7th Coast Guard
                           I* right-of-way.                                                                             District in Miami, Florida.
                           void arecis where the                                                                        Refer to section numbers shown with area
                           square With a " 0 -                                                                          designation.
                           yed.                                        IW21
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                                        Site #50 (RBHC), Rookery Bay Henderson Creek (from chart 11430).












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               SrIE NUMBER 51                                     DESIGNATOR -EVFU

               SITE    FAKA UNION B       AY EVERGLADES, FL

               NOAHNAL-SITE CENTER- 25054@08!N
                                                                  81030.78W-

               LOCATED ON NOS CHART                11430,11429
               SITE ACCESS - The site is access       .ed by driving to Port. of the Isl  ds Re ort south
                                                                                       an       s
               of Naples on U.S. @Highway 41 and by launching at the resort. Proceedsouth along
               a man-made channel        to Fak.a Union Bay.      The   sample   site is on a mangrove
               island near channel marker' 53.

               SITE    DESCRIPTION - Faka Unio,n* Bay is located in the Cape Romano-Ten
               Thousand Islands Aquatic Preserve. An intertiaal reef surrounds a mangrove
               island.  The oysters were abundant, and no observable contamination factors were
               noted.

               OYSTER COLLEM- ONS

               Ye a r I - Year   I, oyster  collections  were   made   on  a'small exposed reef with
                      mangroves. . Station. 1 was. located      30 m    from channel marker 48 on a
                      bearing of 180'. Station 2 was 100 m to the north of Station 1 and 25 m from
                      channel marker 50.     Station 3  -was 100 m southeast of Station L.

               Year IT- Year II,stations were moved slightly to-the s       outh to the submerged reef
                      on the, island at the'end of the channel near. marker 53. Station 1 was at the
                      west end. of the reef.- Station 2 was in. the middle.. Station, 3 was on the east
                      end of the island.

               Years III,  IV, V, -VI, VIT, &   viii   Oyster   collection  stations were the same as
                      those described for Year II.

               Year IX    The site was.not'scheduled to be sampled this year.

                   )IM]ENTCOLLECTIONS

               Years 1, 11, 111, & X -.Sediments' were collected ateach oyster-station among the
                      mangrove Toots.

                                   -V       -I    Sediment  s -were not sampled during these years.
               Years, V, VI, VII, III, &     X

               SAMPLING METHOD

                      Oysters, - hand
                      Sediment - hand

               WATER DEPM - intertidal, 0 to 0.3 meters.




                                                           547









              POSSIBLE CONT        AAIINANTS - No sources of contaminants were identified.

              ENVIRONMENTAL DATA

                                        Salinity          Temperature         Dates Sampled
                                           (1700)               (OC)
                   YEAR 1                  27.3                 27.0          20 February 1986
                   YEAR 11                 27.3                 27.0           2 March 1987
                   YEAR 111                31.0                 21.0          21 February 1988
                   YEAR IV                  -                   25.0          30 January 1989
                   YEAR V                  29.0                 23.0          30 January 1990
                   YEAR VI                 15.0                 24.0          3 February 1991
                   YEAR VII                32.0                 19.0          .27 January 1992
                   YEAR VIII               13.0                 22.0          18 January 1993














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                                                                                       Site #51 (EVFU), Everglades Faka Union Bay.


   DeLorme Mamins Company.               MOP Taken twin Morida Atlas and Gazetteer. Reproduced ith perntission.














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              SITE NUMBER 87                                 DESIGNATOR - FBFO

              SITE - FLAMINGO, FLORIDA BAY, FL

              NOAENAL SITE CENTER - 25008.27N                80055.25V

              L40CATED ON NOS CHART            11451
              SITE ACCESS - The boat can be launched at. the Flamingo Marina boat ramp in
              Flamingo City, in the Everglades National Park. A special collecting permit is
              required from the National Park for this site, and must be obtained.- 2 months in
              advance. This was a new site for Year IX.

              SITE DESCRIPTION - The site. is located within and just outside the small boat
              basin. The stations. are all within 100 m of each other, and can be collected by
              .hand at low tide.

              OYSTERPOLLECTIONS
              Year  IX - Station 1 wa's located "within the boat basin next to the boat ramp. The
                    oysters were collected by hand from the boat - along the concrete bulkheads
                    and wooden pilings. Station. 2 was located - 100 m south of Station 1, in a
                    small boat basin surrounded by tall concrete walls. The oysters were taken
                    by hand from the NE wall by the Visitors Center. Station 3 was located - 80
                    m southwest of Station 2,'along the opposing wall and rock area. The
                    oysters were attached to the rocks and wall. Note - the oysters may be hard
                    to spot, due to the heavy algae growth. along the rocks and concrete walls.

                  04ENT COLLECTIONS

              Years IX - The sediments were of a silty nature, and were collected using a small
                    box-core. The sediments for Stations 2.& 3 were taken next to the oyster
                    stations. Station 1 sediments    were collected from the middle of the boat
                    basin, near the oyster station.

              SAMPLINGMETHOD

                    Oysters   hand
                    Sediment - Box-core

              WATER DEPTH - Intertidal, 0 - 1.0 meters

              POSSIBLE CONTAMINANTS - No sources of contaminants were identified, other
              than from passing boats and ground water run-off from the Visitors Center and
              Restaurant.








                                                       553










           ENVIRONMENTAL DATA

                                Salinity      Temperature    Dates Sampled
                                 (9010)          .(oc)

               YEAR EK           35.0             20.0       25 January 1994







































                                             &54














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              SITE NUMBER 88                                 DESIGNATOR - FBJB

              SITE - JOE BAY, FLORIDA BAY, FL

              NQM[INAL SITE CENTER - 25.'12.53'N             80032.OOV

              LOCATED ON NOS CHART            11451

              SITE ACCESS - The boat can be launched in Florida Bay at the Key Largo Ranger
              Station. To reach the Ranger Station, proceed down, US Hwy I to mile marker
              (MM) 98.7. The Ranger Station is on the right hand side of the road, and the boat
              ramp is behind the station. The ramp is a rough coral one, which ia adequate for
              small boats. Check with the Rangers for an update on.local conditions. The
              o ster. stations are approx. 10 miles awa , at a heading, of 330' at a marked
              @y                                          y
              channel into Joe Bay.

              SITE DESCRIPTION - The site is situated along the cut into Joe Bay, and along
              the'eastern side of Joe Bay.

              OYSTER COLLECTIONS

              Year IX - Stationj1 was located within the cut into Joe 'Bay, Station 2 was located
                    within Joe Bay along the eastern shoreline - 50 m north of Station 1, and
                    Station 3 was. located approx. 50 m north of Station 2 along the same
                    shoreline.   The oysters were all attached to mangrove roots.              The
                    landmarks are the "Closed Area',' signs and the channel marker poles with
                    arrows.


              SEDIMENT COLLECTIONS

              Year IX - -The sediments were collected using a small box-core, near the oyster
                    stations within Joe Bay. The sediments were a silty sand with sea-grass.

              SAMPLING METHOD-

                    Oysters   hand
                    Sediment - box-core.

              WATER DEPTH - Intertidal, 0 - 1.0 meters.

              POSSIBLE CONTAMINANTS - No sources of contaminants were identified, ot           her
              than that from passing boat traffic and fishermen.

              ENVIRONMENTAL DATA
                                      Salinity        Temperature        Dates Sampled
                                        (700)              (0c)

                  YEAR    IX            30.0               23.0         25 January 1994



                                                      559


















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                                    negulations for Ocean Dumping Sit" are
                                 contained In 40 GFR, Parts 220-229. Additional                                                                                           SHOALS AND PASSES
                                 Information concerning the regulations and re-
                                 quiireftients for use of the sites may be obtained                                                         Mormon are advised to use caution. The shoals (dark blue areas L
                                 from Environmental Protection Agency (EPA).                                                                posses (heavy dolled lin*sl were obtained from reports and have
                                                                                                                                            verified by field survey%. Stakes and pilot, marking posses, are not a
                                 Sise U.S. Coast Pilots appendix for addresses
                                 of EPA offices                                                                                             to their frequent change in position.
                                                      NOTE J                                   Marsh                                                                     CALMdN
                                         (protected area: 15    CFR 929)                                                                              Improved channels shown by broW lines                        EVER
                              The following activities are      prohibited within    Key                                                              are subject to shoaling, particularly at the edgft
                                                                                                                                                                                                               (protecte
                           Lorgo National Marine Sanctuary*
                                        Damaging or removing coral;                                                                                                                             Allorsh
                                        Anchoring on coral;
                                        Specirlishing;
                                        Removing historical artifacts:                                                       0
                              The 'Divers Down' flog must be displayed during diving
                           octivhies. Peter to 15 CFR 929 for details of        sanctuary
                            regulations.
                                                                                                                                     J00 Bay                                                         %

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              SITE NUMBER 81                                    DESIGNATOR - BHEF

              SITE - BAHIA HONDA KEY, FL

              NOMINAL SIM CENTER - 24039.52'N                   81016.43'W

              LOCATM ON NOS CELART               11445

              SITE'ACCESS - The bivalve collection site is located directly north of the entrance
              to the Bahia Honda Recreation Area and can be reached by driving to the site and
              walking to the shoreline. The sediment collection site'must be accessed by a boat.

              SITE DESCRIPTION - This site was established in Year VI.. There were no
              oysters present but an alternate bivalve, the smooth-edge jewel box (Chama
              sinuosa) of the family Chamidae, was collected for analysis. The bivalves were
              attached, to the edge of the Yertical face of, a hard parbonate wall which is on the
              northeast side of a U shaped cove.

              OYSTMCOLLECTIONS

              Year   VI - All three stations were collected along the carbonate wall. Station          I
                     was in the center of the. northeast shoreline. Station. 2 was seaward (west)
                     of Station 1. Station 3 was to the inside of the cove tow' ard the road. The site
                     was accessible from the shore and was best sampled using a mask and
                     snorkle.

              Year VII. - The same stations were collected again this year.

              'Year VIII - The site was not scheduled to be sampled this year.

              Year IX -   T.he same stations were sampled as in Years V.I & VIL

                  IDMENT COLLECTIONS

              Year. VI  - Sediments were collected along the       mangrove    shoreline   east of the
                     bivalve sites at latitude 24"40.17'N and longitude 81'1.5.90'W. The site could
                     only be reached by boat traveling through shallow water, This was 0.65 mi
                     east of the bivalve collection sites at the first point of land extending into the
                     bay. Station 1 was at the point and Station 2. was 100 m to the east. Station 3
                     was 100 m to the west. Sediments at the site were primarily detrital.
                     material (mostly, sea . grasses) overlaying fine carbonate sand. After
                     removing the overburden of,decomposing organic material to get to the oozy
                     carbonate/detrital mixture which comprised the "surficial sediment",
                     sediments were obtained at a water depth of 0.2 m.

              -Years  VII, VIII, IX   Sediments were not sampled during these years.





                                                         565











             SAMPLING ME`IHOD

                    Oysters - hand
                    Sediment - hand

             'WATER DEPTH - Subtidal, 0.5, meters

             POSSIBLE CONTANEINANTS - No sources of contaminants were identified.

             ENVIRONMENTAL DATA

                                       Salinity         Temperature        Dates Sampled
                                         0700)               (OC)

                  YEAR VI                40.3                25.0          22 January 1991
                  YEAR VII               36.0                20.0          27 January 1992
                  YEARIX                 35.0                20.0          19 January 1994

































                                                        566



































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                                              PUERTO RICO SITES

              Sr    NUMER 84                                  DESIGNATOR - PRBB

              .-SITE - BAHIA DE BOQUERON, PUERTO RICO, PR

              NONOOKAL SITE CENTER - 18000.44'N               67.010.72W

              LOCATED.ON NOS CHART             25675,25671

              SITE ACCESS - The site is accessible only by boat.    A boat and operator is available
              for r,ent in the town of Boqueron. To reach the site trave    'I from the boat dock in
              Boqueron across Bahia de Boqueron (Boqueron Bay) on, a course of 1500 to- the
              entrance of a smaller bay. Pass through. mouth of the,unnamed bay, turn to a
              heading of 500, and proceed to.the east side of the bay. All three stations are
              located in the small bay system on the, south side of Boqueron Bay

            ..SITE DESCRIPTION - Station 1 was'located along the'east shoreline across from
              the marine police boat dock (2950 back tothe dock). Station 2 was located on the'
              north side of the small island at the near the mouth of the bay. The island was
              located south of -a small channel running,, to the west the A nd west of the main
              channel which runs'. to the southeast. Landmarks from' the site were the
              Boqueron. pier (55') And the beach'cotta,gps (900).. Station 3'Was further west down
              the channel at the end of, the cove.. There were  no visible reference landmarks.

              @OYSTER COLLECTIONS
              Years Vii, VIM, & IX - Oysters at all three stations were collected from the roots
                     of red mangrove trees. Many dead oysters were foundinteirspersed with the
                     live oysters, presumably from     recent  fresh  water inflow. Stations are
                     described in@ the site description section..

              SEDIMENT COLLECITONS

              Year VII - Sediments were located. with. the oysters and were collected among the
                     mangrove roots. in 0. 1 to 1 in of water. Sediments were soft anaerobic black
                     mud over hard reddish brown clay.

              Years VIII & IX -'No sediments were collected during these years.

              SAMPLING

                     Oysters - hand'
                                 ha d or box corer
                     Sediment      n

              WATER DEPTH - intertidal, 0.2 - 0.6 in




                                                        571









             POSSIBLE CONTAMINANTS                 A possible source of contamination was runoff
             from a nearby dump.

             ENVIRONMENTAL DATA

                                       Salinity         Temperature        Dates Sampled
                                         (0/00)              (0c)

                  YEAR VII               .35.0               28.0          11 February 1992
                  YEAR    VIII           36.0                28.7          19 February 1993
                  YEAR IX                36.0                25.0          14 February 1994





































                                                        572














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              SrIE NUMBER 85                                 DESIGNATOR - PRBM

              SITE     BAHIA MONTALVA, PUERTO RICO, PR

              NOAHNAL SITE CENTER - 17058.23rN               66059.43rW

              LOCATED ON NOS CELART            25671

              SITE ACCESS        This site is a walkup site and is reached by auto 'mobile. Travel
              from the La Pa'rguera area north on Highway 304 to Highway 324. Take Highway
              324 toward Ensenada, travel east to the Highway 323 turn off, continue on for
              approximately 200 m on Highway 324 to the first dirt road past the white house
              with a pink roof Turn right and follow the dirt road past the pole barn with the
              ru sty roof and across the salt pond-tothe edge of Bahia Montalva (Montalva Bay).

              SITE DESCRIPTION - All three stations were located along the north shoreline.
              Station 1 was directly south of the pole barn adjacent to the boat anchoring area.
              Station 2.was 50 M@ east of Station 1. - Station 3, was 50 m west, of Station 1.

              OYSTER COLLECTIONS

              Years VII, VIII, & IX - Oysters were collected at the stations -described in the site
                     description. Station 1 oysters- were attached to red mangrove rootsi rubble,
                     PVC and wood pilings'along the shoreline. Station 2 and 3 oysters were all
                     attached to. mangrove roots. Oysters were small and very thin shelled.

              SEDEVIENT COLLECTIONS
             *Year VII - Sediments were      collocated with the oysters. Station 1 was located
                     approximately 5 m west of the oysters at a small boat dock. The sediment
                     was black mud. Station 2 was at the end of the eastern most small boat
                     dock. Sediments were black sandy mud. Station 3 was 2-3 in from the
                     oysters and was collected from around the mangrove roots and in open
                     Patches among the turtle grass beds. Sediments at this site were much
                     sandier and contained some broken shell.
              Years VIII & IX - No sediments were collected. these years'.

                              YMOD
              SAMPLING M[E

                     Oysters -hand
                     Sediment - hand

              WATER DEPT11 - intertidal, .0.2 meter

              POSSIBLE CONTAMINANTS -             There were no obvious sources of contamination,
              the area is very. rural with no, industry nearby.





                                                       577











              ENVIRONMENTAL DATA

                                        Salinity         Temperature        Dates Sampled
                                                              (OC)
                                          (10/00)

                   YEAR VII               36.0                30.3          13 February 1992
                   YEAR VIII              37.0                28.0         20 February 1993
                   YEARIX                 36.0                26.0          13 February 1994










































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                                                                                                                                                                                                                  HYDROGRAPHY
                                                                                                                                                                                      The most recent             bosiC hydrographic surveys used                In
                                                                                                                                                                                   the compilation of this chart were accomplished by 1he
                                                                                                                                                                                   NOAA Ship WHITING, commanded by CDR. Wayne L.
                                                                                                                                                                                   Mobleyin 1968 and 1969. and NOAA ShipMT. MITCHELL,
                                                                                                                                                                                   commanded by CDR.. Wesley V, Hull in 1976.






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              Sm rNTUrVIBER 86                                DESIGNATOR - PRBJ

              SITE - BAHIA DE JOBOS, PUERTO RICO, PR

              NOMINAL SITE CENTER - 17756.33'N                66010.95V

              LOCATED ON NOS CHART             2.56

              SITE ACCESS        This site is located in the east end of Bahia de Jobos (4obos Bay)
              and can only be reachedby boat. -There are no boats for lease in the area so
              arrang Iements must be ma:de' with a local fisherman. - To reach the boat launch
              area travel from Jobos by automobile on Highway 7710 toward Pozuelo, go to the
              second small bay area where boat are anchored. .-By boat go through the
              mangroves into the bay and turn east. Proceed to the east end of the bay to the
              inlet into Laguna de las Mareas.
              SITE DESCRIPTION,-        All three stations were located at"the east end of Bahia de
              Jobbs. Station 1 was approximately 100 m west of. the inlet into Laguna de las
              Mareas on the south shoreline. Station 2 was at the inlet into Laguna de las
              Ma-reas'. Station 3 was 100 m north of the inlet in a small cove. An obvious
              landmark is the electric generation station at the opposite end of the bay near
              Aguirre.

              OYSTER COLLECMONS

              Years VII, VIII, & IX - Oysters were collected at the stations described in the site
                    descriptions.. Oysters at all three stations. were found on the roots of red
                    mangrove trees. Oysters were not abundant.,

              SEDIMENT COLLEMONS

              Year VII -. Sediments sampling stations were collocated with the oysters in 1 to 2
                    in of water andwere collected within 2 to 3 in of the edge of the mangrove
                    roots. StationA sediments were fine grain black silt with some shell hash.
                    Station 2 sediments were fine grained black.silty sand. Sediments from
                    Station 3 were fine grained -black silt. Turtle and manatee grass was
                    abundant throughout the bay starting near the edge of the mangrove roots.

              Years VIII & IX - No sediments were collected during these years.

              SAMPLINGMETHOD

                    Oysters - hand
                    Sediment - box corer

              WATER DEPTH - intertidal, 0. 1 to 0.2 meter

              POSSIBLE CONTAMINANTS - There were no              obvious sources of contamination. -
              Contamination    is possible  from  the  opposite end of the bay from the power









              generation plant. The fisherman who took us to the site said that there had been a
             mercury spill in the bay, but did not remember when.

             ENVIRONMENTAL DATA

                                       Salinity         Temperature        Dates Sampled
                                         (1700)              (OC)

                  YEAR     VII           36.0                27.0          14 February 1992
                  YEAR     VIII          38.0                26.7          22 February 1993
                  YEAR IX                36.0                26.0          15 February 1994









































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                                                                                        Site #86 (PRBJ), Puefto Rico Bahia De Jobos.













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             I
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 I             Field Station Data
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                                                                                               MUSSEL WATCH PROJECT YEAR 9 - FIELD STATION DATA



                                                                             . .....     ..... .....                                                                                  .....
                                                      . ...... . .....
                                                                                             X, a:::,-
                                               . ... .. . ... . ....                                                                                                                                      ...... ......      id
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                                                                                                                                                                                ... ............................




          LMSB     SOUTH BAY LOWER LAGUNA MADRE                               26002.77       97*10.48'      B       12)9/93           H           N          Fay       C. virginica         0.3       19        31           15:27          6:00         PL Isabel       0.34
                                                                              26002.7T       97'10.48'      B       12j9/93           H           N          Fay       C. uirginica         0.3       19        @1           15:27          6-00         Pt. Isabel      0.34
                                                                              26-02.7T       97-10.48'      B       12f9/93           H           N          Fay       C virginica          0.3       .19       31           15:@7          6-00         Pt. Isabel      0.34
          LMPI     PORT ISABEL LOWER LAGUNA MADRE                             26*04.6Z       97*12.OW       B       12tM3             H           N          Fay       C. virginica       o-o.5       21        29           14:09          *5:13        PL Isabel       0.34
                                                                              2.6*04.6Z      97*12.05'      B       121W              H           N          Fay       C. virginica       0-0.5       21        M            14:09.         5:13         PL Isabel       0.34
                                                                              26*04-67       97*12.05'      B       IWM3              H           N          Fay.      C. uirgtnics       0.0.5       21        29           14:09          6:13         PL Isabel       0.34
          LMAC     ARROYO COLORADO LAGUNA MADRE                               26*16.W        97*17.W        B       12tM              H           HNG        FAY       C. uirginica         0.3       22        30           22:49          4:1  0       Padre Is.       0.34
                                                                              261*16.W       97*17.30'      B       12tM              H           HNG        Fay       C. uirginica'        0.3                 30           22:49          4:10         Padre Is.       0.34
                                                                              26*16.W        97*17.30'      B       1218=             H           HNG        Fay       C. virginica,        o.3       22        30           22:49'         4.10         Padre Is.       0.34
          CCNB     NEUCES BAY CORPUS CHRIS71                                                 97*21.W        B       1211093           H           SM       Wilson      C. virginim        0-0.2       13        32.          18:50          .10-38       Aransas P.      0.65
                                                                              27*51AT        97*21.55'      B       12114t93          H           SM       Wilson      C. virginica       0.0.2       13        32           18:50          10:38        Aransas P.      0.65
                                                                              217*51.lr      97M.55'        B       1244(93           H           SM       Wilson      C. virginica       0-0.2       13        32           18:50          10:38        Aransas P.      0.65
          ABLR     LONG REEF ARANSAS BAY                                      28002-96'      96*56.7T       B       12(16(93          D           MNSG     Jobling     C. virginica       0-0.5       16        23           20:06          12.07        Aransas P.      0.5
                                                                              28*02.W        96056.7T'      B       12(16/93          D           MNSG     Jobling     C. uirginica       0.0.5       16        .23          20:06          12-07        Aransas P.      0.5
                                                                              28*02.96'      96*56.77'      B       12(16(93          D           MNSG     Jobling     C. virginica       0-0.5       16        23           20:06          12:07        Aransas P.      0.5
                                                                                                                                                                                   sca           .1   13        24
          MBAR     AYRES REEF MESQUITE BAY                                    28010.16       96049.9W       B       12116/93          H                    Wilson      C. virgin'         0-0,                               19:30          11:23        Aransas P.      0.58
                                                                              28*10.  *15'   96*49.96'      B       12115M3           H                    Wilson      C. uirginica       04.1        13        24           19:30          11:23        Aransas P.      0.58
                                                                              28*10.15'      [email protected]       B       12(IM3            H                    Wilson      C. virginica       0-0.1       13        24           19:30          11:23        Aransas P.      0.58
          SAPP     PANTHER Pr. REEF SAN ANTONIO BAY                           28*14.W        96-42.W        B       12fist93          D           8        Jcb1ing     C. viqinica        0.2-1.5     15        24           19:30          11:23        Pt. O'Con.      0.2
                                                                              28114.W        96-42.W        B       1245M3            D           S        Jobling     C. viyXinica       0.2-1.5     15        24           19:30          11:23        Pt. O'Con.      0.2
                                                                              28*14.W        96042.W        11      12(1"3            D           S        Jobling     C. virginico;      O.Z1.5      Ili       24           19:30          11:23        Pt. OrCon.      0.2
          ESSP     SOUTH PASS REEF ESPIRITU SANTO                             28*17.W        96*37.33'      B       12(16(93         RD           R        Wilson      C. virginica       .0.1-0.5    17        24           20:06          12-07        Pt. O'Con.      0.1'7
                                                                              28018.90'      96*37.33'      B       124610           HD           R        Wilson      C. uirginica       Q.1-0.5     17        27.          20:06          12:07        Pt. O'Con.      0.17
                                                                              28*19.W        96*37.33'      B       l2fl6/93         HD           R        Wilson      C. virginica       0.145@      17        24           20:06          12:07        Pt. OrCon.      0.17
          ESBD     BILL DAYS REEF ESPIRITU SANTO                              28024.86       96*26.2r       B       1216193.          If          SNA      Wilson      C. virginica       0-0.3       16        30           20:06          12:07        Pt. O'Con.      0.17
                                                                              28124.W        96*26.2r       B       12116/93          H           SNA      Wilson      C. virginica       0-0.3       16        30           20:06,         12:07        Pt. OCon.       0.17
                                                                              28*24.W        96@Aff         B       12(16/93          H           SNA      Wilson      C. virginwa        0-0.3       16        30           20.06          12:07        Pt. OCon,       0.17
          MBGP`    GALLINIPPER, PT., MATAGORDA BAY                            28*35.25'      96*34.lr       B       12114(93          D           SMH      Jobling     C. virginica         2.0       15        25           18:50          1068         Pt. Lavaca      0.32
                                                                              28*35.2S       ï¿½6'34.IT       B       12(14M3           D           SMH      Jobling     C. virNinica         2.0       15        25           18.50          10:38        Pt. Lavaca      0.32
                                                                              28-35.26       96-34.1 r      B       121IN93           D           8MH      Jobling     C. uirginica         2.0       15        25           18:50          10:38        Pt. Lavaca      0.32
          MBCB     CARANCAHUA BAY MATAGORDA BAY                              28-39-40'       96-M.18'       B       124"3             H           SR       Jobling     C. virginica       OL_ 0.3     15        25           imb            10:38        Pt. Lavaca      0.32
                                                                             28*39.40'       96*=.18'       B       12(14(93          H           911.     Jobling     C. uirginica       0-0.3       15        25           18:50          10:38        Pt. Lavaca      0.32
                                                                             28139-40'       961M.18'       B       12(14M3           H           SR       Jobling     C. virginica       0-0.3       15        25           1&50           10:38        Pt. Lavaca      0.32
          BRCL     CEDAR LAKE BRAZOS RIVER                                    28*51.W        95*27.W.       B       12t7/93           H           R        Wilson      C. virginica       0-0.3       18        22           22:01          4:06         Freeport        0.34
                                                                              28051.W        [email protected]         B       12J7/93           H           R        Wilson      C. virginica       0-0.3       18        22           22:01          4-06         Freeport        0.34
                                                                                                                                                           Wilson      C. viryinica       0-0.3       18        22           22:01          4:06
                                                                              28*51.W        95 27.83'      13      12t7/0            H           C                                                                                                      Freeport        0.34
          GBOB     OFFATS BAYOU GALVESTON BAY                                 27*17.W        94*50.15'      B       izwm              H           B        Wilson      C. virginica       0-0.5       17        21           23:13          5.55         Galveston       0.35
                                                                              27*17.09       94050.15'      B       IZIM3             H           B        Wilson      C. virginica       0-0.5       17        21           0.13           5.55         Galveston       0.35
                                                                              27*17.C6       94*50.15'      B       12t8/93           H           B        Wilson      C. virginica       0-0.5       17        21           1:13"          5.56         Galveston       0.35
          GBTD     TODD`8 DUMP GALVESTON BAY                                  29*30-W        94153.8T       B       12IM3             D           SM       Jobling     C. virginica         1.5       16        13           23:46          6-36         Texas City      0.35
                                                                              29*30.06'      94*53.82'-     B       1218M             D           SM       Jobling     C. virginica         1.5       16        13           23:46          6:36         Texas City      0.35
                                                                              29*30.W        94153.82'      B       I ZWO             D           SM       Jobling     C. virginica         1.6       16        13           23:46          6:36         Texas City      0.35
          GBSC     SHIP CHANNEL GALVESTON BAY                                 29*42.27       94*59.58'      B       12/7/93           D           SM.      Jobling     C. virginica         1.5       16        12           23-16          5:49         Texas City      0.25
          SLBB     BLUE BUCK POINT SABINE LAKE                                29-47.W        93-r>4.42,     B       i2mm              D           SM       Wilson      C. virginica         1.5'      16        11           13:26          5:27         Sabine          0.64
                                                                              29*47.W        93*54.42'      B       12t9/93           D           SM       Wilson      C. virginic'a        1.5       16        11           13:26          5:27         Sabine          0.64
                                                                              29*47.50;      93*54.42'      B       12/9/93           D           SM       Wilson      C. virginica         1.5       16        11           13:25          5:27         Sabine          0.64




                                                                                                                                                         al,&=0N MMMD1. HW1f&D.T.MNQ5j3-13FwmoFJt-Rmm                  5AKKX1TM     11-81VALVE.19-SMIORW








                                                                                          MUSSEL WATCH PROJECT YEAR 9 - FIELD STATION DATA





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         CLLC    LAKE CHARLES CALCASIEU LAKE                               30'03.4T     93*18.42'      B       1219/93         H           SM       Wilson     C. virginica        0.2-0.5     18        12       12:31          5:43         Mernientau.    0.85
                                                                           30003.42'    93*18.42'      B       12tg/93         H           SM       Wilson     C. uiqinica         0.2-0.5     18        12       12:31          5:43         Mermentam      0.85
                                                                           30103.42'    93*18.42'      B       1219/93         H           SM       Wilson     C. uirginica        0.2-0.5     18        12       12:31          5.43         Mermentau.     0.85
         VBSP    SOUTHWEST PASS VERMILLION BAY                             29*34.W      92102.7V       B       1/11/94         D           SMH      Jobling    C. virginica        2.0         8         3        17:09          9:09         Sw Pass        0.71
                                                                           29*34.60'    92*02.76'      B       1/11/94         D           SMH      Jobling    C. virginiom        2.0         8         3        17:09          9:09         SW Pass        0.71
                                                                           29*34.W      92*02.75'      B       1/11/94         D           SMH      Jobling    C. virginica        2.0         8         3        17:09          9:09         SW Pass        0.71
         CDCL    CAILLOU LAEE CAILLOU LAKE                                 29115.2.T    90155.80'      B       I/12f94         D           SM       Jobling    C. virginica        2.0         10        10       22:11          9:31         Caillou        0.55
                                                                           29*15.25'    90*55.80'      B       1/12/94         D           SM       Jobling    C. uirginica        2.0         10        10       22.11          9:31         Caillou        0.55
                                                                           29*15.25     90*55.W        B       1/12/94         D           SM       Jebling    C. virginica        10          10        10       22:11          9:31         Callon         0.55
         TBLF    LAKE FELICITY TERREBONNE BAY                              29015.W      90124.40'      B       1/11/94         H           SM       Wilson     C. virginica        0-0,5       15        15       21:07          8:26         Timbalier      -0.65
                                                                           29*15.W      90024.40'      B       1/11/94         H           M        Wilson     C. virginica        0-0.5       15        15       21:07          8:26         Timbalier      0.55
                                                                           29*15.W      90*24.40'      B       1/11/94         H           MG       Wilson     C. virginica        0-0.5       15        16       21:07          8:26         Timbalier      0.55
         MRPL    PASS A LIDUTRE MISSISSIPPI RIVER                          29*04.87'    89005.53'      B       1/14/94         D           MS       Jobling    C. virginica        1-1.5       8         2        19:40          7:49         P-a-Loutre     0.34
                                                                           29*04.8T     89*05.5T       B       1/14/94         D           MS       Jobling    C. uirginim         1-1.5       8         2        19-40          7:49         P-a-Loutre     0.34
                                                                           29*04.87     89*05.53'      B       1/14/94         D           MS       Jobling    C. virginica        1-1.5       8         2        19:40          7:49         P-a-Loutre     0.34
         BSSI    SABLE ISLAND BRETON SOUND                                 29*24.11'    89*29.10'      B       1/13/94         D           MS       Jobling    C. viqinica         1.0         10        1        23:33          9:60         Breton S.      0.42
                                                                           29*24.11'    89129.10'      B       1/13/94         D           MS       Jobling    C. virginica        1.0         10        1        23:33          9:50         Breton S.      0.42
                                                                           29*24.11'    89*29.10'      B       1/13/94         D           MS       Jabling    C. virginica        1.0         10        1        23:33          9:50         Breton S.      0.42
         LBMP    MALHEUREUX POINT LAKE BORGNE                              29*52.02'    89*40.70'      B       1/14/94         H           SM       Wilson     C. virginica        0-0.4       10        6        20-40          9:36         N. Orleans     0.22
                                                                           29*51.9W     89*40.71'      B       1/14194         H           SM       Wilson     C. uirginica        0-0.4       10        6        20:40          9:36         N. Orleans     0.22-
                                                                           29*51.9Y     89*40.71'      B       1/14/94         H           SM       Wilson     C. virginica        0-0.4       10        6        20:40          9:36         N. Orleans     0.22
         LBNO    NEW ORLEANS LAKE BORGNE                                   29156.W      89050.10'      B       1/14/94         H           SMP      Wilson     C. virginim         0.2         9         7        20.40          9:36         N. Orleans     0.22
                                                                           29*56.W     .89*50.10'      B       1/14/94         H           SMP      Wilson     C. virginica        0.2         9         7        20:40          9:36         N. Orleans     0.22
                                                                           29*56.60'    89,50.10'      B       I/l4f94         H           SMP      Wilson     C. virginica        0.2         9         7        20:40          9:36         N. Orleans     0.22
         MSPC    PASS CHRISTIAN MISSISSIPPI SOUND                          30*18.IT     89019.6T       B       1/15/94         D           NSH      Jobling    C. virginica        2.5         6         18       11:02          22:09        Bay S. Lou     0.46
                                                                           30*18.12'    89119.67'      B       1/15/94         D           NSH      Jobling    C. vironica         2.5         6         18       11:02          22:09        Bay S. Lou     0.46
                                                                           30*18.12'    89*19.67'      B       1115/94         D           NSH      Jobling    C. virginica        2.5         6         18       11:02          22:09        Bay S. I@au    0.46
         MSPB    PASCAGOULA BAY MISSISSIPPI SOUND                          30*20.W      88*36.10'      B       1/15/94         D           SR       Jobling    C. virginica        1.5         11        18       9-29           19:57        Pascagoula     0.42
                                                                           30*20.03'    88*36.10'      B       1/15/94         D           MS       Jobling    C. viMinica         1.5         11        18       9:29           19:57        Pascagoula     0.42
                                                                           30020.03'    88136.10'      B       1/16/94         D           SR       Jobling    C. virginica        1.5         11        18       9:29           19:57        Pascagoula     0.42
         MBCP    CEDAR POINT REEF MOBILE BAY                               30*18.70'    88*08.00'      B       1/15/94         H           NS       Wilson     C. virginica        .0.2-1.2    11        20       1:59           11:28        Mobile         0.3
                                                                           30*18."      88*08.15'      B       1/15/94         H           NS       Wilson     C. virginica        0.2-1.2     11        20       1:69           11:28        Mobile         0.3
                                                                           30*18.64!    88*08.15'      B       1/15/94         H           NS       Wilson     C. virginica        0.2-1.2     11        20       1:59           11:28        Mobile         0.3
         MBHI    HOLLINGERS ISLAND MOBILE BAY                              30033.W      88*04.50'      B       1/15/94         D           MSN      Wilson     C. virginica        2.0         9         7        1:59           11:28        Mobile         0.3
                                                                           30*33.W      88004.50'      B       1/1&"           D           MSN      Wilson     C. virginica        2.0         9         7        1:59           11:28        Mobile         0.3
                                                                           30*33.W      88*04.60'      B       1/15/94         D           MSN      Wilson     C. virginica        2.0         9         7        1:69           11.28        Mobile         0.3
         PBPH    PUBLIC HARBOR PENSACOLA BAY                               3002A.6Y     87*11.42'      B       1/16t94         H           N        Wilson     C. uirginica        0.5         10        24       0-52           10:07        Pensacola      0.52
                                                                           30*24.6S     87011.42'      B       1/16/94         H           N        Wilson     C. virginica        0.5         10        24       0:52           10-07        Pensacola      0.52
                                                                           30*24.6T     87*11.42'      9       1/16/94         H           N        Wilson     C. virginica        0.5         10        24       0:52           10-07        Pensacola      0.52
         PBIB    INDIAN BAYOU, PENSAC01A BAY                               30131.W      87*06.70'      B       I A 6f94        D           SM       Wilson     C. virginica        2.0         11        23       0:52           10-07        Pensacola      0.52
                                                                           30*31.W      87006.7W       B       1/16/94         D           SM       Wilson     C. virginica        2.0         11        23       0:52           10-07        Pensacola      0.52
                                                                           30*31.W      87*06.7W       B       1116/94         D           SM       Wilson     C. virginica        2.0         it        23       0:52           10:07        Pensacola      0.52
         CBJB    JOE'S BAY CHOCTAWATCHEE BAY                               30*24.6X     86129.45'      B       1/17/94         H       SBRMN        Wilson     C. virginica        0.1-0.5     11        24       16:64          10:26        Destin         0.13
                                                                           30*24.67     86*29.45'      B       i/17/94         H       SBRMN        Wilson     C. virginica        0.1-0.5     11        24       16:64          10:26        Destin         0.13
                                                                           30*24.6T     86*29.45'      B       1/17194         H       SBRMN        Wilson     C. virginica        0.1-0.5     11        24       16:54          10:26        Destin         0.13
         CBBL    BEN'S BAYOU CHOCTAWAHATCHEE BAY                           30-27.15'    86-28.73'      B       1/17/94         H           CB       Wilson     C. virginica        0.1-0.5     13        25       16:54          10,26        Destin         0.13
                                                                           30*27.15'    86*28.73'      B       1/17/94         H           CB       Wilson     C. virginica        0.1-0.5     13        25       16:54          10:26        Destin         0.13
                                                                           30*27.11T    86*28.73'      B       1/17/94         H           CB       Wilson     C. virginica        0.1-0.5     13        25       16:54          10:26        Destin         0.13



                          BorrOft [email protected],Et-CONCRE7Yj4.SAND,G.GRASS.HtVSKC.MAYR.RMW.B-RUBBMK.MPAALGAET.ROUISYN.PFAT COUECITONWIliolk. H-HMD.TdT0No8XwD=GKA.RA= SAM"ITM: B-81VALVE,9-MMENT
                                'm            m                            m              m            m             m                            m             M                                          'm









                                                                                               MUSSEL WATCH PROJECT YEAR 9 - FIELD STATION DATA





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          CBPP     POSTIL POINT CHOCTAWHATICHEE BAY                            30-M.W        86-&.73'        B       1116A)4          H            B        Jobling     C. virginica       0.0.3       6         24          0:25            11:27         Destin         0.24
                                                                               30*28.85' 86*28.73'           B       Ih6,94           H            B        Jobling     C virginica        0-0.3       6         24          0.25            11:27         Destin         0.24
                                                                               30*28.W      .96128.137       B       1116194          H            B        Jcb1ing     C. virginica       0-0.3       6         24          0:25            11:27         Destin         0.24
          CBBB     BOGGY BAYOU CHOCTAWAHATCHEE BAY                             30-30.19      86-a.65'        B       Ih6/94           H            E        Jobling     C. virginica        0.5        7         17'         0:25            11:27         Destin         0.24
                                                                               30030.18!     86029.65'       B       1/16194          H            E        Jobling     C. virgin'          0.5
                                                                                                                                                                                   wa                  7         17          0:25            11:27         Destin         0.24
                                                                               30*30.18'     86*29.65'       B       1/16/94          H            E        Jobling     C. virginica        0.5        7         17          0:25           .11:27         Destin         0.24
          PCIA)    LrITLE OYSTER PANAMA CITY                                   *15.19'       85-40.95-       B       1/17/94          H            M        Jobling     C. virginica        0.2        12        10          17:15           9:26          Lyn Haven      0.34
                                                                               30015.19'     85*40.95'       B       1/17/94          H            M        Jobling     C. virginica        0.3,       12        25          17:15           9:26          Lyn Haven      0.34
                                                                               30*15.IW      85040.95'       B       1/17/94          H            M        Jobling     C. virginim         0.2        12        10          17:15           9:26          Lyn Haven      0.34
          AESP     SPRING CREEK APPALACHEE BAY                                 30*03.76      84*19.37'       B       1/18/94          H            SR       Wilson      C. virginica        0.5        15        13          17:68           0:15          Bald Pt.       0.57
                                                                               30*03.75!     84119.37'       B       1/18/94          H            SR    '  Wilson      C. vi i ica
                                                                                                                                                                              rgin          0.5        15   ,    13.         17:58           0.15          Bald Pt.       0.57
                                                                               30*03.75'     84*19.37        B       1/18/94          H            SR       Wilson      C. virginica        0.5        15        13          17:58           0:15          Bald Pt.       0.57
          CKBP     BLACK POINT CEDAR KEY                                       29*12.28'     83*04.34!       B       1/19/94          H            MSR      Wilson      C. virginica        1.0        4         19          17:47           0:26          Cedar Key      0.69
                                                                               29*12.28'     83*04.U         B       1/19/94          H            MSR      Wilson      C. virginica        1.0        4         19          17:47           0:20          Cedar Key      0.69
                                                                               29*12.28'     83*04.34'       B       1/19/94          H            MSR      Wilson      C. virginica,       1.0        4.        19,         17:47           0:20          Cedar Key      0.69
          TBPB     PAPYS BAYOU TAMPA BAY                                       27*50.6T      82*36.62'       B       If20194          H            SR       Wilson      C. virginica        0.5        14        28          19:65           2:50          'St. Peters.   0.55
                                                                               27*60.5T,     82136.62'       H       1/20/94          H            SR       Wilson      C. virginica        0.5        14        28          i9:55           2:60          St. Peters.    0.55
                                                                               27*50.5T      82.*36.62'      B       1/20/94          H            SR       Wilson      C. virginica        0.5        14        28          19:55           2:50          St. Peters.    0.55
          TBCrT    OLD TAMPA BAY TAMPA BAY                                     28401.49      82137.9V        B       1=194            H            NSR      Wilson      C virginica         0.2        13        2           21:33           4:45          Safety H.      0.67
                                                                                                                                                   SR       Wilson      C. virgin'                                                           4:45          Safety H.      0.67
                                                                               28*01.48'     8T37.95'        B       14@0/94          H                                            ica      0.2        13        2           21:33
                                                                               .28*01.48'    82*37.95'       B       1/20194          H            SR       Wilson      C. virginica        0.2        13        11          21:33           4:45          Safety H.      0.67
   >      TBKA     KNIGHT AIRPORT TAMPA BAY                                    27*54.52'     82*27.27'       B       1/20/94          H            NBH       Fay        C. virginica        @0.2       14        30          20:02           3:16          Hillebrgh.     0.67
                                                                               27*64.52'     82*27.27'       B       1/20/94          H            NBH       Fay        C virginica         6.2        14        30          20:02           3:16          Hillabrgh.     0.67
                                                                               27164.5Z      82*27.27'       B       1/20/94          H            NBH       'Fay       C. virginica        0.2        14        30          2D:02           @16           Hillebrgh.     0.67
          TBNP     NAVAREZ PARK TAMPA BAY                                      27147.28'     82*45.28'       B       1120/94          H            N        Wilson      C. virginica        0.3        12        32          18:15           1:32          Madeira B.     0.65
                                                                               .27*47.29,    82045.28'       B       It20/94          H            N        Wilson      C. virginica        0.3        12        32          18:15           1:32          Madeira B.     0.55
                                                                               27*47.2W      82*45.28'       B       1/20/94          H            N        Wilson      C. virginica        0.3        12        32          18:16           1:32          Madeira B.     0.55
          TBHB     HILLSBOR      OUGH BAY TAMPA BAY                            27051.28'     82*23.75'       B       IM/94            H
                                                                                                                                                            Wilson      C. virginica       0-0.6       15        16          20.53           4:30          Hillebrgh.     0.75
                                                                               27*51.2W      82123.75'       B       1/21/94          H                     Wilson      C. virginica       0-0.6       15        15          20:53           4-30,         Hillebrgh.     0.75
                                                                               27*51.29      8=35'           B       1/21/94          H                     Wilson      C. virginim        0-0.6       15        15          20:53           4:30          Hillsbrgh.     0.75
          TBMK     MULLET KEY BAYOU TAMPA BAY                                  27*37.2W      82*43.62'       B       1121/k           H                     Wilson      C. uirginica       0-0.5       15        33          18:37           3:20          SL Pete B.     0.52
                                                                               27*37.2W      82*43.62'       B       If21/94          H                     Wilson      C. uirginica       0-0.5       15        33          18:31           3:20.         St. Pete B.    0.52
                                                                               27*37.28'     82143.62'       B       1/21/94          H                     Wilson      C. virginica       6-0.5       15        33          18:37           3:20          St. Pete B.    0.52
          CBBI     BIRD ISLAND CHARL07TE HARBOR                                26*30.79      82*02.18'       B       1/21/64          D            SR        Fay        C. virginica        0.8        16        26          20:27           4.30          Pineland       0.5
                                                                               26,30.71      82102.19        B       IM/94            D            SR        Fay        C. virginica        0.8        16        26          20-27           4:30          Pineland       0.5
                                                                               26*30.73'     82002.18'       B       IM/94            D            SR        Pay        C. virginica        0.8        16        26          26:27           4-30          Pineland       0.5
          RBHC     HENDERSON CREEK ROOKERY BAY                                 26*01.W       81 *44.2D'      B       IM/94            H            SN        Fay '      C. virginica       0-0.4       18        34          19:23           2:34          Marco          0.51
                                                                               26*01.W       81*44.20'       B       1/21194          H            SN        Fay        C. virginica       0-0.4       18        34          19:23           2:34          Marco          0.51
                                                                               .26001.50'    810".20'        B       1/21/94          H            SN        Fay        C. virginica       0-0.4       18        34          19:23           1-34          Marco          0.51
          FBFO     FLAMINGO FLORIDA BAY                                        25*08.2T      80*55.25'       SB      1 NNN            H            N        Wilson      C. uirginiba       0-1.0       20        35          1-02            9-49          Flamingo       0.88
                                                                               25*08.27      80*55.25'       SB      1/26/94          H            N        Wilson      C. virginica       0-1.0       20        35          1-02            9:49          Flamingo       0.88
                                                                               25*08.27      80*65.25'       SB      1/26194          H            N        Wilson      C. uirginica       0.1.0       20        35          1:02            9:49          Flamingo       0.88
          FBJB     JOE BAY FLORIDA BAY                                         25112.ST      80*32.00'       SB      1/25/94          H            NG       Wilson      C. uirginica       0-1.0       23        3D          1-02            9:49          Flamingo       0.88
                                                                               25*12.53'     80*32.00'       SB      1/2"4            H            NG       Wilson      C. virginica       0-1.0       23        30          1:02            9:49          Flamingo       0.88
                                                                               25*12.53'     80*32.00'       SB      Ir25194          H            NG       Wilson      C. virginica       0-1.0       23        30          1:02            9:49          Flamingo       0.88
          BHKF     BAHIA HONDA KEY FLORIDA                                     24039.52'     81*16.43'       B       1119/94          H            NGP       Fay        C. uirginica        0.5        20        35          14:00           7:42          B.Honda X      0.29
                                                                               24*39.5Z      81*16.43'       B       1/19/94          H            NGP       Fay        C. virginica        0.5        20        35          14:00           7:42          B.Honda K      0.29
                                                                               24*39.5T      81*16.43'       B       1/19/94          H            NGP       Pay        C. virginica        0.5        20        35          14:00           7:42          B.Honda K      0.29


                             BOTMbL [email protected]@'MCWM.N-SAM,G.GRAMX.HAM.C-OAY.It.REEVJ3-RUBBLZJE-PaMPA.ALME.T.RDaTSYI-PFAT COUZZWN hMMC,. H.WXoTwT01Mj)-DRWGEJ7^RNM SAKRAWM: B-MVALVR.5.SMNFNr








                                                                  MUSSEL WATCH PROJECT YEAR 9 - FIELD STA71ON DATA





                                                     ..............                                                                ......

                                                                               . ....  . . . . . .



      PRBB BAHIA DE BOQUERON PUERTO RICO               18*00.4@f 67*10.72'  B     2114194     H        m      Wils6n  C vir&ica     0-0.3   26     35       10,50       17:16   San Joan     0.33
                                                       18100."   67010.7Z   B     2/14194     H        m      Wilson  C. virginica  0-0.3   25     35       10-60       17:16   San Juan     0.33
                                                       18*00."   67*10.72'  B     2114/94     H        m      Wilson  C. virginica  0-0.3   25     35       10,50       17:16   San Juan     .0.33
      PRBM BAHIA MONTALVA PUERTO RICO                  17*58.2Z  66159.437  B     2A 3194     H        TB     Wilson  C. virginks   0-0.3   26     36       2:58        6:67    San Juan     0.37
                                                       17*68.=   66*59.43'  B     2/13/94     H        TH     Wilson, C. virginica  0-0.3   26     36       2:58        6:67    San Juan     0.37
                                                       17*158.2Z 66*59.43'  B     2JI3/94     H        TB     Wilson  C virginica   0-0.3   26     36       2:58        6:57    San Juan,    0.37
      PRBJ BAHIA DE JOBOS PUERTO RICO                  17056.=   66110.W    B     V16t%       14       TN     Wilson  C. virginica  0-0.3   26     36       0:23        7:66    San Juan     0.3
                                                       17*56.3T  66110.95'  B     2/16/94     H        TN     Wilson, C. virginica  0-0.3   26     36       0:23        7:66    San Juan     0.3
                                                       17*56.&T  66*10.95'  B     2115/94     H        TN     Wilson  C. virginica  0-0.3   26     36       0:23        7:56    San Juan     0.3

















































                   BOMM S-MMI bt-MM.E.CONCWMN-SMD.Q.GRASS.H-HASKC-aAY.R.REW.El.RUBBLEX-XMPA.M.GAE.T.RDUMX.PEAT ODUZCROM MMM&. H.WM.T-rV0NG&[).DF=QEJt-RAPZ NkMATM! B-181VALM64-Mm,




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 I                  Appendix B
 I                Final Positions
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           1    LMSB       SOUTH BAY                           LOWER LAGUNA MADRE                      TX         26002.77'        97010.48'        26002.58          97010.49
                           PORTISABELL                         LAGUNA MADRE                            TX         26004.62'        97012.05'
           78-             ARROYO COLORADO                     LAGUNA MADRE                            TX         26'16.80'        97017.30'
           2    CCN        NEUCESBAY                           CORPUS CHRISTI                          TX_        27051.17'        97021.55'                                           27051.24'        97021.47'
           3    CCIC       INGLESIDE COVE                      CORPUS CHRISTI                          TX         27050.28         97014.28         27050.22'         97014.24'        27050.31".       97014.28'
          .53   CCBH       BOAT HARBOR                         CORPUS CHRISTI                          TX         27*50.17'        97022.72'
           4    ABLR       LONG REEF                           ARANSAS BAY                             TX         28002.96'        96056.77'        28003.38'         96054.40'        .218003.96'      96057.79'
           54   ABHI       HARBORISLAND                        ARANSAS BAY                             TX         27050.33'        97004.52'
           5    CBCR       COPANO REEF                         COPANO BAY                              TX         28008.47'        97*07.67'        28*08.50'         97007.68'        28008.57'        97007.68'
           6    MBAR       AYRESREEF                           MESQUITE BAY                            TX         28,010.15'       96049.95'        28010.10'         .96049*.90'      28009.82'        96048.96'
           7    SAPP       PANTHER PT. REEF                    SAN ANTONIO BAY                         TX         28014.00'        96042.55'        28014.10'         96042.50'        28*13.95'        96042.54'
           8    SAMP.      MOSQUITO POINT                      SAN ANTONIO BAY                         TX         28*20.65'        96042.78'        28020.57'         96042.69'        28.'20.64'       96042.77'
           9    ESSP       SOUTH PASS REEF                     ESPIRITU SANTO                          TX         28017.90'        96037.33'        28017.83'         96037.50'        28017.90'        96037.30'
           10   ESBD       BILL DAYS REEF                      ESPIRITU SANTO                          TX         28024.85'        96026.27'        28024.66'         96026.65'        28025.02'        96025.86'
          .11   MBLR       LAVACA RIVER MOUTH                  MATAGORDA BAY                           TX         29039.80'        96034.83'        29039'.62'        96034.72'        29039.79'.       96034.891
           12   MBGP       GALLINIPPER PT.                     MATAGORDA BAY                           TX         28035.25'        9603417          28035.13'         96034.23'        28035.20'        96*34.21'
           13   MBTP       TRES PALACIOS BAY                   MATAGORDA BAY                           TX         28039.50'        96'13.45'        28039.43'         96*13.25'        28039.66'        96013.33'
  t@0    1-4    MBEM       EAST MATAGORDA                      MATAGORDA BAY                           TX         28042.67'        95053.00'        28042.64'         95052.96'        28042.67'        95053.04'
           55   MBDI       DOG ISLAND                          MATAGORDA BAY.                          TX         28038.28'        96000.15,
           56   MBCB       CARANCAHUA BAY                      MATAGORDA BAY                           TX         28039.40'        96023.18'
           72   BRCL       CEDAR LAKES                         BRAZOS RIVER                            TX         28051.50'        95027.83'        28051.46'         95027,75'        28051.50'        95027.75'
           57   BRFS       FREEPORT SURFSIDE                   BRAZOS RIVER                            TX         28055.25'        95020.33'        28055.31'         95o2O.56'        28055.25'        95'20.35'
           18   GBCR       CONFEDERATE REEF                    GALVESTON BAY                           TX         29015.75'        94054.88'        29015.71'         94'54.76'        29015.74'        94054.94'
           17   GBHR       HANNA REEF                  -GALVESTON BAY                                  TX         29028.85'        94044.00'        29028.58'         94043.22'        29028.86'        94044.34'
           58   GBOB       OFFATTS BAYOU                       GALVESTON BAY                           TX         29017.0.8'       94050.15'--      29016.96'         94050.08'        29017.07'        94050.15'
           59   GBSC       SHIP CHANNEL                        GALVESTON BAY                           Tx.        29042.27'        94059.58'        29041.97'         94059.37'        29042.42'        94059.59'
           16   GBTD       TODUS DUMP                          GALVESTON BAY                           TX         29030.06'        94053.82'        29030.06'         94653.82'        29030.13'        94053.86'
           15   JGBYC      YACHT CLUB                          GALVESTON BAY                           TX         29037.30'                         29037.39'         94059.35'        29037.36'        94059.73'

           19   SLBB       BLUE BUCK POINT                     SABINE LAKE                           TX-LA        29047.50'        93054.42'                                           29047.09'        93054.36'
           20   CLSJ       ST. JOHNS ISLAND                    CALCASIEU LAKE                          LA         29049.83'        93023.00'        29049.85'                          29049.65'        93023.04'
           60   CLLC       LAKE CHARLES                        CALCASIEU LAKE                          LA         30*03.42'        93018.42'                                           30'03.51'        93018.43'
           21   JHJH       JOSEPH HARBOR BAYOU                 J. HARBOR BAYOU                         LA         29037.75'        92045.75'                                           29037.96'        92045.78'
           22   VBSP       !SOUTHWEST PASS                     VERMILLION BAY                          LA         29034.60'        92002.75'        29034.45'        .92002.91-
           23   ECSP       SOUTH POINT                         EAST COTE BLANCHE                                  29028.50'-1      91048.00'
                !
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          24   ABOB       OYSTER BAYOR                     ATCHAFALAYA BAY                       LA       29014.40'       91008.10'       29014.51'       91008.13'       29014.40'        91*08.08'
          25   CLCL       CAILLOU LAKE                     CAILLOU LAKE                          LA       29015.25'       90155.80'       29015.30'       90056.02'
          26   TBLB,      LAKE BARRE                       TERREBONNE BAY                        LA       29015.60'       90035.70'       29015.56'       90035.77'       29015.60'        90035.76'
          27   TBLF       LAKE FELICITY                    TERREBONNE BAY                        LA       29015.80'       90024.40'       29015.84'       90024.06'       29015.81'        90023.88'
          28   BBSD       BAYOU ST. DENIS                  BARATARIA BAY                         LA       29024.18'       89059.75'       29024.15'       89059.85'       @29024.05'       89059.82'
          29   BBMB,      MIDDLE BANK                      BARATARIA BAY                         LA       29ol6.55'       89056.53'       29016.56'       89056.64'       29016.55'        89056.49'
          61   BBTB       TURTLE BAY                       BARATARIA BAY                         LA       29030.67'       90005.00'
          30   BSBG       BAY GARDERNE                     BRETON SOUND                          LA       29008.69'       89025.67'       29008.64'       89025.66'       29008.68'        89025.69'
          31   BSSI       SABLEISLAND                      BRETON SOUND                                   29*04.87'       89*05.53'       29004.90'       89005.54'       29004.87'        89005.53'
          32   -LBMP      MALHEUREUX POINT                 LAKE BORGNE                           LA       29*24.11'       89029.10'       29024.12'       89029.21'       29024.11'        89029. 10'
          62   LBNO       NEW ORLEANS                      LAKE BORGNE                           LA       29035.90'       89037.25'       29035,94'       89037.40'       29*35.90'        89*37.27
          63   LPGO       GULF OUTLET                      LAKE PONCHARTRAIN                     LA       29*52.02'       89040.70'       29052.02'       89040.88'       29051.99'        89040.71'
          64   MRPL       PASS A LOUTRE                    MISSISSIPPI RIVER                     LA       29056.60'       89050.10'
          65   MRTP       TIGER PASS                       MISSISSIPPI RIVER                     LA       30002.44'       89002.57'


          33   MSPC       PASS CHRISTIAN                   MISSISSIPPI SOUND                     Ms       30018.87'       89018.05'       30*18.13'       89019.70'
          34   MSBB       BILOXI BAY                       MISSISSIPPI SOUND                     Ms       30023.55'       88051.45'       30023.57'       88051.50'       30023.55'        88051.46'
          36   MSPB       PASCAGOULA BAY                   MISSISSIPPI SOUND                     Ms       30020.03'       88036.10'       30020.44'       88035.55'       30020.70'        88035.45'


          36   MBCP       CEDAR POINT REEF                 MOBILE BAY                            AL       30018.70'       88008.00'       30018.62'       88'08.24'       30018.64'        88008.15'
          66   MBHI       HOLLINGERS ISLAND                MOBILE BAY                            AL       30033.80'       88004.50'       30033.73'       88004.58'       30033.93'        88'04.59'
          79   MBDR       DOG RIVER                        MOBILE BAY                            AL       30035.50'       88002.72'


          67   PBPH       PUBLIC HARBOR                    PENSACOLA BAY                         FL       30024.63'       87011.42'       30*24.39'       87011.41'       30024.62'        87011.43'
          37   JPBIB      INDIAN BAYOU                     PENSACOLA BAY                         FL       30031.00'       87006.70'       30030.86'       87006.66'       30031.06'        87006.68'
          80   PBSP       SABINE POINT                     PENSACOLA BAY                         FL       30020.80'       87009.10'       30020.59'       87009.12'       30020.67'        87009.03'
          73   CBJB       JOESBAYOU                        CHOCTAWHATCHEE BAY                    FL       30024.62'       86029.45'       30024.63'       86029.43'       30024.63'        86-i9.46'
          38   CBSR       OFF SANTA ROSA                   CHOCTAWHATCHEE BAY                    FL       30024.78'       86012.25'       30024.83'       86012.25'       30024.70'        86012.26'
          82   CBBL       BENS LAKE                        CHOCTAWHATCHEE BAY                    FL       30027.16'       86032.45'       30027.16'       86032.34'
          39   CBPP*      POSTIL POINT                     CHOCTAWHATCHEE BAY                    FL       30028.85'       86028.73'       30028.76'       86028.62'       30*28.74'        86028.75'
          83   CBBB       BOGGYBAYOU                       CHOCTAWHATCHEE BAY                    FL       30030.18'       86029.650       30030.16'       86029.46'       30030.11'        8629.65'-
          74   PCLO       L17TLE OYSTER BAY                PANAMA CITY                           FL       30015.19'       85040.95'       30015.22'       85040.84'       30015.17'        85040.92'
          68   PCMP       MUNICIPAL PIER                   PANAMA CITY                           FL       30009.00'       85039.80'       30009.05'       85039.84'       30009.05'        85039.80'
          40   SAWB       WATSON BAYOU                     ST. ANDREW BAY                        FL       30008.53'       85037.92'       30008.51'       85037.84'       30*08.48'        85037.91'
          4
               ---D--r-                                    i                                I    FL -T974-0-.45@                                                                             5004.51'
            1  AP B DRYBAR                                 APALACHICOLA BAY                                               85004.40'       29040.40'       85004.51'       29040.44'        8

















          42   APCP        CAT PT. BAR                        APALACHICOLA BAY                       FL         29043.45'        84053.05'        29043.35'        84053.32'        29*43.46'        84063.22'
          75   AESP        SPRING CREEK                       APALACHEE BAY                          FL         30003.75'        84019.37'        30003.67'        84019.47'        30003.75'        84019.37'
          69.  SRWP        WEST PASS                          SUWANEE RIVER                          FL         29019.75'        83010.45'
          43   CKBP        BLACKPOINT                         CEDAR KEY                              FL         29012.32'        .83*04.25'       29012.28'        83004.34'        29012.32'        83004.28'
          70   TBOT        OLD TAMPA BAY                      TAMPABAY                               FL         28001.48'        82037.95'
          44   TBPB        PAPYS BAYOU                        TAMPABAY-,                             FL         27050.53'        82036.62'        27050.46'        82036.74'
          45   TBHB        HILLSBOROUGH BAY                   TAMPABAY                               FL         27051.28'        82023.75'        .27051.15'       82023.80'        27051.31'        82023.7V
          46   TBCB        COCKROACH BAY                      TAMPABAY                               FL         27040.55'        .82030.56'       .27040.44'       82030.70'.       27040.53'        82030.62'
          47   TBMK        MULLET KEY BAYOU                   TAMPABAY                               FL         27037.28'        82043.62'        27037.24!        82043.59'        27037.31'        82043.57'
          77   TBKA        KNIGHT AIRPORT                     TAMPABAY                               FL         27054.46'        87027.29'        27054.42'        87027.38'        27054.52'        82027.27'
          76   TBNP        NAVAREZPARK                        TAMPABAY                               FL         27647.28,        82046.28                                           27047.33'        82045.19'
          48   CBBI.       BIRD ISLAND                        CHARLOTTE HARBOR                       FL         26030.73'        82*02.18'        26030.74'        82002.19'
          71   CBFM        FORT MEYERS                        CHARLOTTE HARBOR                       FL         26033.50'        81055.37'        26033.55'        81055.751        26633.19'        81055.75'
          49   NBNB        NAPLES BAY                         NAPLES BAY                             FL         26006.78'        81047.15'        26006.61'        81047.14'        26006.75'        81047.14'
          50   RBHC        HENDERSON CREEK.                   ROOKERYBAY                             FLi        26001.50'        81044.20,        26001.40,        81044.15'        26'01.48'        81044.26'
          87   FBFO        FLAMINGO.                          FLORIDA BAY                            FL         25 08.27'        80 55.25'
               FBJB;       JOE BAY                            FLORIDA BAY                            FL         25 12.53'        80 32.00'
          51   EVFU        FAKA UNION BAY                     EVERGLADES                             FL         25054.08'        81030.78'        25053.'84'       81030.80'        25054.12'        81030.877
          81   BHKF        BAHIA HONDA KEY.                   FLORIDA KEYS                           FL         24039,52         81016-43         24'39.52         81o16.28         -


          84   PRBB        BAHIA DE BOQUERON                  PUERTO RICO                            PR            000.44'       67010.72'                                          18000.44'
          85   PRBM        BAHIA MONTALVA                     PUERTO RICO                            PR            *58.28'       66'59.43'
          86   PRBJ        BAHIA DE JOBOS                     PUERTO RICO                            'PR        17056.33'        66010.95'


           name change in 1992
                                                                                                            @
                                                                                                                27050' 53'
                                                                                                                27-51. 28'
                                                                                                                27. 0. 55,
                                                                                                                27 3 7 228
                                                                                                            ME




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