Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification - GOVPUB-C13-bc65b90bbf79ed9e7fa930620199813b | Content Details | GovInfo

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Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

Summary

Publication Title: Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

Series Title: NIST Special Publications

Personal Author: Kralj, Jason.

Publication Number: NIST special pub; NIST SP; 260-225

Date Issued: 2022

Subject: Pathogens
Bacteria
DNA
Metagenomics

Government Author: Commerce Department, National Institute of Standards and Technology (NIST) .

Category: Executive Agency Publications

SuDoc Class Number: C 13.

Abstract: Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8 buffer. Each component consists of one skirted 0.5-mL tube containing approximately 100 uL of DNA solution. Each tube is labeled and sealed with a removable screwcap. Here, we have documented the production, analytical methods, and statistical evaluation of realizing this RM.